scholarly journals Histopathological study of different organs of charles foster strain rat under the exposure of Pueraria tuberosa

2019 ◽  
Author(s):  
Harsh Pandey ◽  
Shivani Srivastava ◽  
Surabhi Singh ◽  
Yamini BhusanTripathi
Keyword(s):  
Adrenal Gland ◽  
Water Extract ◽  
Morphological Alteration ◽  
Histopathological Study ◽  
Morphological Alterations ◽  
Pueraria Tuberosa ◽  
Heart Size ◽  
Selection Of ◽  
Cardiac Fibers

AbstractObjectiveThe present study was undertaken to investigate the safe doses of Pueraria tuberosa water extract (PTWE) on different organs.MethodologyHaematoxylin and eosin staining was used to study the morphological alterations in heart, intestine, testis, adrenal gland and spleen. Followed the OECD guidelines 407 of repeated toxicity with respect to the selection of dose and days for different organs. The selected doses of PTWE were 250, 500, 1000 and 2000 mg/kg bw and the durations selected were 7, 14, 21 and 28 days.ResultAccording to the obtained results, we have found no any types of adverse alteration in cardiac fibers of the heart, size and shapes in crypts and villi of intestine, semeniferous tubules and spermatozoa count were normal in testis, all three zones of adrenal gland were normally identified and no any adverse sign of pulps in spleen was seen in all treated groups of PTWEConclusionThere was no any types of adverse morphological alteration found in any organs. The drug PTWE are safe at 1000 mg/kg bw upto 28 days and 2000 mg/ kg bw upto 21 days respectively.

scholarly journals A novel general approach to eucaryotic mutagenesis functionally identifies conserved regions within the adenovirus 13S E1A polypeptide.

1986 ◽  
Vol 6 (5) ◽  
pp. 1487-1496 ◽  
Author(s):  
D Kimelman
Keyword(s):  
Shuttle Vector ◽  
Point Mutations ◽  
Mutant Gene ◽  
Adenovirus Type ◽  
Cellular Morphology ◽  
Morphological Alteration ◽  
Morphological Alterations ◽  
E1a Gene ◽  
Adenovirus Type 5

A new approach to the isolation of mutations in mammalian genes was developed which permits both the selection of infrequently occurring mutants that alter the cellular morphology of recipient cells and the rapid reisolation of the mutant gene. The adenovirus type 5 13S early region 1a (E1a) gene was mutagenized in vitro with sodium bisulfite and then efficiently transferred into cells with a retrovirus shuttle vector. Three classes of mutants of the 13S E1a gene product were isolated, each of which induced a distinct morphological alteration. The mutant E1a gene was reisolated from each cell line, and the precise nucleotide changes were determined. The E1a-induced morphological alterations were further examined by the construction of single and double point mutations within different regions of the polypeptides by utilizing the amino acid substitutions obtained from the original mutants. The results suggest that each of the three regions of highly conserved amino acids within the E1a 13S polypeptide has a distinct role in the alteration of cellular morphology and the activation of gene expression.

scholarly journals IMPORTANCE OF CLASSICAL MORPHOLOGY IN THE DIAGNOSIS OF MYELODYSPLASTIC SYNDROME

2015 ◽  
Vol 7 ◽  
pp. e2015035 ◽  
Author(s):  
Rosangela Invernizzi ◽  
Federica Quaglia ◽  
Matteo Giovanni Della Porta
Keyword(s):  
Molecular Techniques ◽  
Classification Systems ◽  
World Health ◽  
Hematopoietic Stem ◽  
Morphological Alterations ◽  
Diagnosis And Classification ◽  
The Moment ◽  
Health Organization ◽  
Selection Of

Myelodysplastic syndromes (MDS) are hematopoietic stem cell disorders characterized by dysplastic, ineffective, clonal and neoplastic hematopoiesis. MDS represent a complex hematological problem: differences in disease presentation, progression and outcome  have necessitated the use of classification systems to improve diagnosis, prognostication and treatment selection. However, since a single biological or genetic reliable diagnostic marker has not yet been discovered for MDS, quantitative and qualitative dysplastic morphological alterations of bone marrow precursors and of peripheral blood cells are still fundamental for diagnostic classification. In this paper World Health Organization (WHO) classification refinements and current minimal diagnostic criteria proposed by expert panels are highlighted and related problematic issues are discussed. The recommendations should facilitate diagnostic and prognostic evaluations in MDS and selection of patients for new effective targeted therapies. Although in the future morphology should be supplemented with new molecular techniques, the morphological approach, at least for the moment, is still the cornerstone for the diagnosis and classification of these disorders.

scholarly journals Cathaemasia hians infection in Black stork in Slovakia: morphological and histopathological study

2015 ◽  
Vol 52 (4) ◽  
pp. 316-322 ◽  
Author(s):  
A. Königová ◽  
G. Hrčková ◽  
L. Molnár ◽  
P. Major ◽  
M. Várady
Keyword(s):  
Inflammatory Response ◽  
Lamina Propria ◽  
Histopathological Examination ◽  
Fibrous Tissue ◽  
Mechanical Damage ◽  
Histopathological Study ◽  
Intermediate Hosts ◽  
Tissue Samples ◽  
Morphological Alterations ◽  
Black Stork

SummaryCathaemasia hians is an obligate trematode parasite of Black storks that are on the List of protected birds in Europe. In the present study, adult trematodes were isolated from the Black stork post mortem and morphological study revealed C. hians species. In total, 10 worms were found in the oesophagus and the ventriculus of the bird. Histopathological examination of the tissue samples of oesophagus, proventriculus and ventriculus was performed on paraffin sections using a set of staining procedures. The sporadic lesions were seen in the tela submucosa of oesophagus containing connective tissue mast cells, eosinophils and heterophils and some foci were surrounded by the fibrous tissue. In addition, a few inflammatory nodules had larval-like material inside, probably being of the same species. There were no visible morphological alterations in the epithelial layer of lamina propria mucosae of proventriculus, rich in goblet cells as well as in the tela submucosa. Majority of trematodes were localized in the ventriculus, where the lamina propria mucosae was damaged or disrupted sporadically. In these sites, in the tela submucosa, a various food-originated inorganic/organic material and eggs of C. hians were deposited, stimulating a weak inflammatory response. Nodules containing larvae were not observed in any of ventriculus tissue layers. This study demonstrated, for the first time, infection with adults of C. hians trematode in the Black stork nesting in Slovakia. The presence of larvae and eggs in the tissues of the upper gastrointestinal tract of bird was associated with mild inflammatory response but feeding behaviour of adult worms in the ventriculus probably contributed to the enhanced susceptibility of the lamina propria mucosae to mechanical damage by inorganic material. Although larval stages have not yet been documented in the intermediate hosts in Slovakia, our report indicates that the life cycle of C. hians might occur in Central Europe.

Structural, functional, and metabolic correlates in ischemic hearts: effects of substrates

1981 ◽  
Vol 240 (3) ◽  
pp. H391-H398
Author(s):  
D. Feuvray
Keyword(s):  
Morphological Alteration ◽  
Mechanical Function ◽  
Morphological Alterations ◽  
Metabolic Substrates ◽  
Heart Ischemia ◽  
Initial Reduction ◽  
Rat Hearts ◽  
Chain Acyl ◽  
Whole Heart ◽  
Severe Deterioration

The ultrastructure, function, and metabolism of isolated rat hearts perfused under control or ischemic conditions were investigated. Either both glucose (11 mM) or glucose and palmitic acid (1.5 mM) were used as metabolic substrates. A 60-min period of whole-heart ischemia, i.e., a 60% initial reduction in coronary flow, resulted in a more dramatic morphological alteration in those hearts receiving palmitate compared to those receiving glucose as the only substrate. In ischemic hearts receiving palmitate, intramitochondrial osmiophilic amorphous densities of both rounded and elongated types were observed. These amorphous densities did not develop in ischemic hearts receiving glucose alone over the same period of ischemia. Such morphological alterations were associated with a more severe deterioration of mechanical function in the presence of palmitate. Both ischemic conditions resulted in increased tissue levels of acyl esters of CoA and carnitine, but the rise in levels of long-chain acyl carnitine was about two times greater in those ischemic hearts receiving palmitate.

A novel general approach to eucaryotic mutagenesis functionally identifies conserved regions within the adenovirus 13S E1A polypeptide

1986 ◽  
Vol 6 (5) ◽  
pp. 1487-1496
Author(s):  
D Kimelman
Keyword(s):  
Shuttle Vector ◽  
Point Mutations ◽  
Mutant Gene ◽  
Adenovirus Type ◽  
Cellular Morphology ◽  
Morphological Alteration ◽  
Morphological Alterations ◽  
E1a Gene ◽  
Adenovirus Type 5

A new approach to the isolation of mutations in mammalian genes was developed which permits both the selection of infrequently occurring mutants that alter the cellular morphology of recipient cells and the rapid reisolation of the mutant gene. The adenovirus type 5 13S early region 1a (E1a) gene was mutagenized in vitro with sodium bisulfite and then efficiently transferred into cells with a retrovirus shuttle vector. Three classes of mutants of the 13S E1a gene product were isolated, each of which induced a distinct morphological alteration. The mutant E1a gene was reisolated from each cell line, and the precise nucleotide changes were determined. The E1a-induced morphological alterations were further examined by the construction of single and double point mutations within different regions of the polypeptides by utilizing the amino acid substitutions obtained from the original mutants. The results suggest that each of the three regions of highly conserved amino acids within the E1a 13S polypeptide has a distinct role in the alteration of cellular morphology and the activation of gene expression.

scholarly journals Morphological alterations of the parotid gland of rats maintained on a liquid diet

2003 ◽  
Vol 14 (3) ◽  
pp. 172-176 ◽  
Author(s):  
Soraya Coelho Leal ◽  
Orlando Ayrton de Toledo ◽  
Ana Cristina Barreto Bezerra
Keyword(s):  
Parotid Gland ◽  
Liquid Diet ◽  
Morphological Alteration ◽  
Control Group ◽  
Parotid Glands ◽  
Experimental Animals ◽  
Morphological Alterations ◽  
Gland Weight ◽  
Solid Diet ◽  
Experimental Group

The purpose of this study was to analyze the morphological alterations that occurred in the parotid glands of rats maintained on a liquid diet compared to a solid diet. Thirty-six animals were randomly divided into two groups. The control group received a solid diet, and the experimental group received a liquid diet. The animals were killed after 8, 15 and 30 days. The glands were prepared for inclusion in paraffin and analyzed with a light microscope. The results showed a statistically significant reduction of the parotid gland weight of the animals from the experimental group compared to the control group at 15 and 30 days. The strongest morphological alteration displayed was the presence of cytoplasm vacuoles on the parotid glands of the animals maintained on the liquid diet. Specific stain techniques for glycoproteins and mucopolysaccarides could not identify the substances inside the vacuoles observed in the experimental animals. We conclude that a liquid diet caused atrophy of the parotid gland after 15 and 30 days.

PRELIMINARY PHYTOCHEMICAL SCREENING OF POLYSACCHARIDES CONTENT FOR SELECTION OF HIGH QUALITY PLANTING MATERIALS: Eurycoma longifolia

2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Mohd Zaki, A. ◽  
Nor Fadilah, W. ◽  
Abdul Rashid, L. ◽  
Nurnadiah, R. ◽  
Mohd Radzi, A. ◽  
...  
Keyword(s):  
Water Extract ◽  
Provenance Trial ◽  
Phytochemical Screening ◽  
High Quality ◽  
Actual Weight ◽  
Eurycoma Longifolia ◽  
Polysaccharide Content ◽  
Root Part ◽  
Planting Materials ◽  
Selection Of

Provenance trial of Eurycoma longifolia is currently being carried out in order to provide necessary information for breeding strategies, which is crucially needed for the production of high quality planting materials. Assessment of polysaccharides content in the root part of the plant is taken among the important characteristics in the selection of the best provenance. Polysaccharides compound is chosen as bioactive marker due to the non-toxicity nature of the compound and potential pharmacological properties possessed. Polysaccharides compound of the root part is extracted by using water extract procedure from nine selected provenances (Johor, Melaka, Pahang A, Pahang B, Pahang D, Perak, Pulau Pinang, Selangor and Terengganu) of E. longifolia collected throughout forest reserves of Peninsular Malaysia. The analysis of the actual weight of the compound is conducted using phenol-sulfuric acid method by UV-VIS and High Performance Liquid Chromatography (HPLC) is used for the detection of structural components of the polysaccharides compound. The assessment showed that polysaccharides content in the root part of Terengganu provenance significantly has the highest with 1.06 % actual weight of polysaccharides (out of 30 g extracted dry roots). Meanwhile, there are four provenances showed undetectable range of polysaccharide content which were Melaka, Pahang A, Pulau Pinang and Selangor. In a nutshell, based on the preliminary assessment of polysaccharide content alone, Terengganu provenance has the potential to be declared among the best provenances that has high quality. The provenance trial also has taken into considerations several important parameters such as the growth performances variation and the tolerance of the plants to pests and diseases besides the phytochemical screening. Provenance trial of E. longifolia is still ongoing, data for various parameters are still being collected, and thus it is still too early to derive a conclusion of which is the best provenance.

scholarly journals Mapping and QTL analysis of the barley population Tallon × Kaputar

2003 ◽  
Vol 54 (12) ◽  
pp. 1155 ◽  
Author(s):  
M. Cakir ◽  
D. Poulsen ◽  
N. W. Galwey ◽  
G. A. Ablett ◽  
K. J. Chalmers ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Genetic Map ◽  
Qtl Analysis ◽  
Water Extract ◽  
Reference Points ◽  
Hot Water ◽  
Quality Traits ◽  
Software Packages ◽  
Hot Water Extract ◽  
Selection Of

A genetic map of barley with 224 AFLP and 39 simple sequence repeat (SSR) markers was constructed using a doubled haploid (DH) mapping population from a cross between the varieties Tallon and Kaputar. Linkage groups were assigned to individual barley chromosomes using the published map locations of the SSR markers as reference points. This genetic map was used to identify markers with linkage to agronomic, disease, and quality traits in barley. The population, which comprised 65 lines, was tested in a range of environments across Australia. Quantitative trait loci (QTLs) analyses were performed using software packages MapMaker, MapManager, and Qgene. Significant associations with markers were found for several traits. Grain yield showed significant association with regions on chromosomes 2H, 3H, and 5H over a range of sites throughout Australia. Regions on chromosomes 2H and 3H explained 30% and 26% of variation in lodging, respectively. Among quality traits, diastatic power was associated with regions on chromosomes 1H, 2H, and 5H (R2 = 37%). Hot water extract was associated with a region on chromosome 6H and a marker not assigned to a chromosome (R2 = 45%). There were also environment-specific QTLs for the traits analysed. The markers identified here present an opportunity for marker assisted selection of lines for these traits in barley breeding programs.Mapping and QTL analysis of Tallon × Kaputar

scholarly journals Herbal tablet of Pueraria tuberosa water extract suppresses the alloxan induced liver damage and hyperglycemia in rats

2019 ◽  
Author(s):  
Harsh Pandey ◽  
Shivani Srivastava ◽  
Yamini Bhusan Tripathi
Keyword(s):  
Blood Glucose ◽  
Morphological Changes ◽  
Water Extract ◽  
Liver Function Tests ◽  
Diabetic Control ◽  
Dependent Manner ◽  
Pueraria Tuberosa ◽  
Group 3 ◽  
Group 2 ◽  
Liver Tissues

AbstractAimTo study the protective response of herbal formulation (tablets) of Pueraria tuberosa water extract (PTAB) on alloxan induced rat diabetic model.MethodologyAlloxan (120 mg/kg bw) was injected intraperitonially. Rats were divided into three groups: group 1 as normal, group 2 as diabetic control and group 3 were given PTAB upto 14 days. Blood glucose and liver function tests were done using their respective kits. Hematoxylene and eosin staining was done to evaluate the morphological changes in liver tissues. Through immunohistochemistry, we have checked the protein expression of VEGF, MMP9 and ki67.ResultPTAB significantly decreases blood glucose level in a time dependent manner up to 14 days. As compared to diabetic control, PTAB decreases SGOT, SGPT and alkaline phosphates after 14 days of treatment. In diabetic control, the morphology of liver tissues were found damaged due to deformed hepatocytes and dilated lobules. Most of the hepatocytes after PTAB treatment were comparatively found similar to normal rats tissues, along with dilated blood vessels and normalized liver lobules. In addition to these results, PTAB suppresses the expressions of VEGF and MMP 9.ConclusionPTAB was found to act as an effective hypoglycemic agent. In addition to this, PTAB also found to inhibit inflammation and apoptosis of liver. Thus, can be taken as a potential drug in liver diseases.

scholarly journals Histopathological study of different organs of Charles foster rats under the exposure of Pueraria tuberosa

2021 ◽  
Vol 11 (1) ◽  
pp. 100
Author(s):  
Harsh Pandey ◽  
Shivani Srivastava ◽  
Mohan Kumar ◽  
Yamini Bhusan Tripathi
Keyword(s):  
Histopathological Study ◽  
Pueraria Tuberosa
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