scholarly journals The ecological and epidemiological consequences of reproductive interference between the vectors Aedes aegypti and Aedes albopictus

2019 ◽  
Author(s):  
Robert S Paton ◽  
Michael B Bonsall
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Reproductive Interference ◽  
Larval Competition ◽  
Vector Ecology ◽  
Functional Responses ◽  
Vector Borne Diseases ◽  
Competitive Mechanisms ◽  
Vector Borne ◽  
Mosquito Population Dynamics

AbstractVector ecology is integral to understanding the transmission of vector-borne diseases, with processes such as reproduction and competition pivotal in determining vector presence and abundance. The arbovirus vectors Aedes aegypti and Aedes albopictus compete as larvae, but this mechanism is insufficient to explain patterns of coexistence and exclusion. Inviable interspecies matings - known as reproductive interference - is another candidate mechanism. Here, we analyse mathematical models of mosquito population dynamics and epidemiology which include two Aedes-specific features of reproductive interference. First, as these mosquitoes use hosts to find mates, reproductive interference will only occur if the same host is visited. Host choice will, in turn, be determined by functional responses to host availability. Second, females can become sterilised after mis-mating with heterospecifics. We find that a species with an affinity for a shared host will suffer more from reproductive interference than a less selective competitor. Costs from reproductive interference can be “traded-off” against costs from larval competition, leading to competitive outcomes difficult to predict from empirical evidence. Sterilisations of a self-limiting species can counter-intuitively lead to higher densities than a competitor suffering less sterilisation. We identify that functional responses and reproductive interference mediate a concomitant relationship between vector ecological dynamics and epidemiology. Competitors with opposite functional responses can maintain disease where human hosts are rare, due to vector coexistence facilitated by a reduced cost from reproductive interference. Our work elucidates the relative roles of the competitive mechanisms governing Aedes populations and the associated epidemiological consequences.

scholarly journals The ecological and epidemiological consequences of reproductive interference between the vectors Aedes aegypti and Aedes albopictus

2019 ◽  
Vol 16 (156) ◽  
pp. 20190270 ◽  
Author(s):  
Robert S. Paton ◽  
Michael B. Bonsall
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Reproductive Interference ◽  
Larval Competition ◽  
Vector Ecology ◽  
Vector Borne Diseases ◽  
Behavioural Responses ◽  
Competitive Mechanisms ◽  
Vector Borne ◽  
Mosquito Population Dynamics

Vector ecology is integral to understanding the transmission of vector-borne diseases, with processes such as reproduction and competition pivotal in determining vector presence and abundance. The arbovirus vectors Aedes aegypti and Aedes albopictus compete as larvae, but this mechanism is insufficient to explain patterns of coexistence and exclusion. Inviable interspecies matings—known as reproductive interference—is another candidate mechanism. Here, we analyse mathematical models of mosquito population dynamics and epidemiology which include two Aedes -specific features of reproductive interference. First, as these mosquitoes use hosts to find mates, reproductive interference will only occur if the same host is visited. Host choice will, in turn, be determined by behavioural responses to host availability. Second, females can become sterilized after mis-mating with heterospecifics. We find that a species with an affinity for a shared host will suffer more from reproductive interference than a less selective competitor. Costs from reproductive interference can be ‘traded-off’ against costs from larval competition, leading to competitive outcomes that are difficult to predict from empirical evidence. Sterilizations of a self-limiting species can counterintuitively lead to higher densities than a competitor suffering less sterilization. We identify that behavioural responses and reproductive interference mediate a concomitant relationship between vector ecological dynamics and epidemiology. Competitors with opposite behavioural responses can maintain disease where human hosts are rare, due to vector coexistence facilitated by a reduced cost from reproductive interference. Our work elucidates the relative roles of the competitive mechanisms governing Aedes populations and the associated epidemiological consequences.

scholarly journals Analyses of Insecticide Resistance Genes in Aedes aegypti and Aedes albopictus Mosquito Populations from Cameroon

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 828
Author(s):  
Borel Djiappi-Tchamen ◽  
Mariette Stella Nana-Ndjangwo ◽  
Konstantinos Mavridis ◽  
Abdou Talipouo ◽  
Elysée Nchoutpouen ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
Aedes Albopictus ◽  
Expression Profiles ◽  
Resistance Mechanisms ◽  
Vector Borne Diseases ◽  
Aedes Mosquitoes ◽  
Control And Prevention ◽  
Vector Borne ◽  
First Time

The emergence of insecticide resistance in Aedes mosquitoes could pose major challenges for arboviral-borne disease control. In this paper, insecticide susceptibility level and resistance mechanisms were assessed in Aedes aegypti (Linnaeus, 1762) and Aedes albopictus (Skuse, 1894) from urban settings of Cameroon. The F1 progeny of Aedes aegypti and Aedes albopictus collected in Douala, Yaoundé and Dschang from August to December 2020 was tested using WHO tube assays with four insecticides: deltamethrin 0.05%, permethrin 0.75%, DDT 4% and bendiocarb 0.1%. TaqMan, qPCR and RT-qPCR assays were used to detect kdr mutations and the expression profiles of eight detoxification genes. Aedes aegypti mosquitoes from Douala were found to be resistant to DDT, permethrin and deltamethrin. Three kdr mutations, F1534C, V1016G and V1016I were detected in Aedes aegypti populations from Douala and Dschang. The kdr allele F1534C was predominant (90%) in Aedes aegypti and was detected for the first time in Aedes albopictus (2.08%). P450s genes, Cyp9J28 (2.23–7.03 folds), Cyp9M6 (1.49–2.59 folds), Cyp9J32 (1.29–3.75 folds) and GSTD4 (1.34–55.3 folds) were found overexpressed in the Douala and Yaoundé Aedes aegypti populations. The emergence of insecticide resistance in Aedes aegypti and Aedes albopictus calls for alternative strategies towards the control and prevention of arboviral vector-borne diseases in Cameroon.

scholarly journals Dengue and Chikungunya Fever: Oral Manifestations

2019 ◽  
Vol 3 (4) ◽  
pp. 141-143
Author(s):  
Indrajeet Singh ◽  
Naresh Sharma
Keyword(s):  
Aedes Aegypti ◽  
Incubation Period ◽  
South Asian ◽  
Signs And Symptoms ◽  
Oral Manifestations ◽  
Female Mosquito ◽  
Asian Countries ◽  
Breeding Sites ◽  
Vector Borne Diseases ◽  
Vector Borne

The vector borne diseases of dengue and chikungunya mainly affect the African and South Asian countries. Its prevalence increases in the summer and monsoon seasons due to an  increase in breeding sites and External Incubation Period (EIP) of the virus. Primarily caused by an infectious bite of the Aedes aegypti female mosquito, this disease has a variety of systemic signs and symptoms. However, the oral manifesatations might be ignored. This review aims to enlightens the healthcare researchers with knowledge regarding the oral manifestations of dengue and chikungunya.

scholarly journals Regulation of midgut cell proliferation impacts Aedes aegypti susceptibility to dengue virus

2018 ◽  
Author(s):  
Mabel L. Taracena ◽  
Vanessa Bottino-Rojas ◽  
Octavio A.C. Talyuli ◽  
Ana Beatriz Walter-Nuno ◽  
José Henrique M. Oliveira ◽  
...  
Keyword(s):  
Virus Infection ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Denv Infection ◽  
Pathogen Transmission ◽  
Intestinal Lumen ◽  
Midgut Cell ◽  
Vector Borne Diseases ◽  
Cellular Processes ◽  
Vector Borne

AbstractAedes aegypti is the vector of some of the most important vector-borne diseases like Dengue, Chikungunya, Zika and Yellow fever, affecting millions of people worldwide. The cellular processes that follow a blood meal in the mosquito midgut are directly associated with pathogen transmission. We studied the homeostatic response of the midgut against oxidative stress, as well as bacterial and dengue virus (DENV) infections, focusing on the proliferative ability of the intestinal stem cells (ISC). Inhibition of the peritrophic matrix (PM) formation led to an increase in ROS production by the epithelial cells in response to contact with the resident microbiota, suggesting that maintenance of low levels of ROS in the intestinal lumen is key to keep ISCs division in balance. We show that dengue virus infection induces midgut cell division in both DENV susceptible (Rockefeller) and refractory (Orlando) mosquito strains. However, the susceptible strain delays the activation of the regeneration process compared with the refractory strain. Impairment of the Delta/Notch signaling, by silencing the Notch ligand Delta using RNAi, significantly increased the susceptibility of the refractory strains to DENV infection of the midgut. We propose that this cell replenishment is essential to control viral infection in the mosquito. Our study demonstrates that the intestinal epithelium of the blood fed mosquito is able to respond and defend against different challenges, including virus infection. In addition, we provide unprecedented evidence that the activation of a cellular regenerative program in the midgut is important for the determination of the mosquito vectorial competence.

scholarly journals Integrated management of vector borne diseases in conditions of humanitarian crisis with emphasis on vector control

2020 ◽  
Author(s):  
Εμμανουήλ-Αλέξανδρος Φωτάκης
Keyword(s):  
Vector Control ◽  
Aedes Albopictus ◽  
Culex Pipiens ◽  
Leishmania Donovani ◽  
Integrated Management ◽  
Phlebotomus Papatasi ◽  
Humanitarian Crisis ◽  
Vector Borne Diseases ◽  
Vector Borne

Οι εντομομεταδιδόμενες ασθένειες αποτελούν ένα από τα σημαντικότερα προβλήματα που αντιμετωπίζει η ανθρωπότητα πλήττοντας δυσανάλογα φτωχούς και περιθωριοποιημένους πληθυσμούς. Κατά την τελευταία δεκαετία, η Ελλάδα και γείτονες χώρες είναι αντιμέτωπες με την εμφάνιση/επανεμφάνιση σημαντικών ασθενειών που μεταδίδονται από κουνούπια και φλεβοτόμους - θέτοντας σημαντικές προκλήσεις για τη δημόσια υγεία. Μέσα σε αυτό το έντονο επιδημιολογικό περιβάλλον, χιλιάδες πρόσφυγες διαμένουν επί του παρόντος σε προσωρινές δομές υπό ανεπαρκείς συνθήκες διαβίωσης, ενδεχομένως αντιμετωπίζοντας αυξημένο κίνδυνο έκθεσης στα εν λόγω νοσήματα. Η προστασία της δημόσιας υγείας έναντι των εντομεταδιδόμενων ασθενειών βασίζεται σε μεγάλο βαθμό σε προγράμματα καταπολέμησης των φορέων με την χρήση εντομοκτόνων. Ωστόσο, ένα παγκόσμιο πρόβλημα που σχετίζεται με την εκτεταμένη χρήση περιορισμένου αριθμού διαθέσιμων εντομοκτόνων είναι η ανάπτυξη ανθεκτικότητας στα εντομοκτόνα σε πληθυσμούς φορέων, απειλώντας την αποτελεσματικότητα των παρεμβάσεων. Η ανάπτυξη κατάλληλων προγραμμάτων καταπολέμησης (βασιζόμενα σε δεδομένα επιτήρησης) και η διαχείριση της ανθεκτικότητας στα εντομοκτόνα αποτελούν επιτακτική ανάγκη για τον αποτελεσματικό έλεγχο των φορέων και των ασθενειών που μεταδίδουν. Βασικές προϋποθέσεις για αυτό περιλαμβάνουν, ο εντοπισμός μοριακών μηχανισμών που προσδίδουν ανθεκτικότητα, η ανάπτυξη ολοκληρωμένων συστημάτων επιτήρησης με έμφαση σε περιοχές αυξημένης επιδημιολογικής σημασίας και η μετατροπή των δεδομένων επιτήρησης σε επιχειρησιακή γνώση/πληροφορία. Η διδακτορική μου μελέτη πραγματοποιήθηκε σε τρία ερευνητικά μέρη. Στο 1ο μέρος, αναλύθηκαν κουνούπια Culex pipiens – που είναι ο κύριος φορέας του ιού του Δυτικού Νείλου, για μεταλλαγές που παρέχουν ανθεκτικότητα στο diflubenzuron (DFB), ένα από τα πιο ευρέως χρησιμοποιούμενα προνυμφοκτόνα. Αναλύσεις αλληλούχισης σε πληθυσμούς από την Ιταλία αποκάλυψαν την παρουσία μιας νέας μεταλλαγής, της I1043F, στο γονίδιο συνθάσης της χιτινής (CHS) των κουνουπιών. H συγκεκριμένη μεταλλαγή (η οποία έχει βρεθεί επίσης σε έντομα γεωργικής σημασίας και έχει συσχετιστεί με πολύ υψηλά επίπεδα ανθεκτικότητας στο DFB) βρέθηκε να είναι σημαντικά υψηλότερη σε Cx. pipiens κουνούπια που επιβίωσαν σε υψηλές δόσεις DFB. Αναλύσεις για την παρουσία/συχνότητα της I1043F και των μεταλλαγών I1043L και I1043M που έχουν εντοπιστεί παλαιότερα, σε Cx. pipiens κουνούπια από διάφορες χώρες της Μεσογείου κατέγραψαν υψηλές συχνότητες μεταλλαγών σε πληθυσμούς από την Ιταλία, φτάνοντας το 93,3% για την I1043M, το 64,8% για την I1043L και το 10% για την I1043F, εγείροντας μεγάλη ανησυχία για τα προγράμματα καταπολέμησης κουνουπιών στην Ευρώπη και επισημαίνοντας την αναγκαιότητα ανάπτυξης κατάλληλων προγραμμάτων διαχείρισης της ανθεκτικότητας. Επίσης ανέλυσα σκνίπες-φορείς λεϊσμανίασης που συλλέχθηκαν από την Ελλάδα και την Τουρκία για την παρουσία και τη συχνότητα μεταλλαγών kdr που σχετίζονται με ανθεκτικότητα στα πυρεθροειδή (τα πιο ευρέως χρησιμοποιούμενα ακμαιοκτόνα). Η μεταλλαγή L1014F (η οποία σε ομοζυγωτία έχει συσχετιστεί με ανθεκτικούς φαινοτύπους στα πυρεθροειδή σε πολλά έντομα) ανιχνεύθηκε σε δείγματα Phlebotomus papatasi από την Sanliurfa, Τουρκία σε αλληλική συχνότητα 48% με τα L1014F/L1014F ομόζυγα άτομα να αντιπροσωπεύουν το 32% του πληθυσμού. Αυτή είναι η πρώτη αναφορά μεταλλαγών ανθεκτικότητας στον κύριο φορέα λεϊσμανίασης P. papatasi, προκαλώντας ιδιαίτερη ανησυχία για την καταπολέμηση της λεϊσμανίασης. Στο 2ο μέρος: Αναπτύχθηκε ένα δίκτυο επιτήρησης φορέων / παθογόνων σε τέσσερα κέντρα φιλοξενίας προσφύγων στην Ελλάδα. Καταγράφηκαν σημαντικά είδη κουνουπιών και σκνιπών - φορείς παθογόνων καθώς και υψηλά ποσοστά μόλυνσης από Leishmania donovani και L. tropica σε δείγματα σκνιπών, δείχνοντας πως οι τοπικοί προσφυγικοί πληθυσμοί αντιμετωπίζουν αυξημένο κίνδυνο μόλυνσης από εντομομεταδιδόμενες ασθένειες. Επιπλέον, αναλύσεις στα συλλεχθέντα άτομα κουνουπιών/σκνιπών για την παρουσία/συχνότητα μεταλλαγών kdr και ανθεκτικότητας στο DFB, αποκάλυψε την παρουσία μεταλλαγών kdr σε τοπικούς πληθυσμούς Cx. pipiens και Aedes albopictus, αποτελώντας πιθανή απειλή κατά της αποτελεσματικότητας πυρεθροειδών εντομοκτόνων στα υπό μελέτη κέντρα. Η καθιέρωση προγραμμάτων επιτήρησης / καταπολέμησης εντομομεταδιδόμενων ασθενειών σε οικισμούς προσφύγων και η πραγματοποίηση στοχοθετημένων δράσεων για την διασφάλιση επαρκών συνθηκών διαβίωσης, αποτελούν απαραίτητες ενέργειες για την προστασία των προσφυγικών πληθυσμών. Στο 3ο μέρος : Σχεδίασα το VectorMap-GR: ένα ανοιχτής πρόσβασης επιχειρησιακό εργαλείο διαχείρισης εντομολογικών δεδομένων, που υποστηρίζει την παραγωγή επιχειρησιακών GIS χαρτών και γραφημάτων. Το σύστημα επιτρέπει επίσης συγκρίσεις και συσχετίσεις των εντομολογικών δεδομένων με σύνολα επιδημιολογικών δεδομένων. Η ενσωμάτωση του νέου εργαλείου σε προγράμματα καταπολέμησης μπορεί να βοηθήσει στην ιεράρχηση περιοχών σε σχέση με την ευπάθεια μετάδοσης ασθενειών, την ανάπτυξη έγκαιρων και κατάλληλων αποκρίσεων ελέγχου/καταπολέμησης καθώς και την αξιολόγηση τρεχόντων και προηγούμενων προγραμμάτων καταπολέμησης.

scholarly journals Larvicidal Activity of Elytraria acaulis against Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae)

2020 ◽  
Author(s):  
Soorya Sukumaran ◽  
Rajan Maheswaran
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Culex Quinquefasciatus ◽  
Probit Analysis ◽  
Human Beings ◽  
Vector Borne Diseases ◽  
Blood Sucking ◽  
Leaf Powder ◽  
Vector Borne ◽  
Chemical Pesticides

Background: Mosquitoes are blood sucking arthropods and serve as vectors of many diseases causing serious health problems to human beings. Culex quinquefasciatus and Aedes aegypti were responsible for Filariasis and Dengue. Syn­thetic pesticides were effective against mosquitoes as well as main sources of environmental pollution and most of them are immunosuppressant. Botanicals were widely used as insecticides, growth disruptors, repellents, etc. The aim of this research was to determine larvicidal properties of powdered leaf, Elytraria acaulis against late third or early fourth in­star larvae of Cx. quinquefasciatus and Ae. aegypti. Methods: Larvae of Cx. quinquefasciatus and Ae. aegypti were tested at various concentrations of 100, 120, 140, 160, 180 and 200mg/100ml and mortality was recorded after 24h. The LC50 values of the E. acaulis leaf powder were calcu­lated by Probit analysis. Results: The plant powder exhibited strong larvicidal activity against Cx. quinquefasciatus with LC50 value of 116.07mg/100ml against Ae. aegypti 124.25mg/100ml respectively. The result indicated that the plant powder of E. acaulis showed potential larvicidal activity against Cx. quinquefasciatus and Ae. aegypti. Conclusion: The overall findings of the present investigation suggested that the E. acaulis highly effective against Cx. quinquefasciatus and Ae. aegypti larvae. Elytraria acaulis may be used as an alternative to synthetic chemical pesticides for control of vectors to reduce vector borne diseases and did not harm to total environment.

scholarly journals Yellow fever and dengue: a threat to Europe?

2010 ◽  
Vol 15 (10) ◽  
Author(s):  
P Reiter
Keyword(s):  
Natural History ◽  
Yellow Fever ◽  
Aedes Albopictus ◽  
Vector Borne Diseases ◽  
History Of ◽  
Vector Borne ◽  
Broad Overview

The introduction and rapidly expanding range of Aedes albopictus in Europe is an iconic example of the growing risk of the globalisation of vectors and vector-borne diseases. The history of yellow fever and dengue in temperate regions confirms that transmission of both diseases could recur, particularly if Ae. aegypti, a more effective vector, were to be re-introduced. The article is a broad overview of the natural history and epidemiology of both diseases in the context of these risks.

scholarly journals Young and Old Sugar Apple (Annona squamosa Linn) Leaf Extracts As an Aedes aegypti Larva Insecticide

2019 ◽  
Author(s):  
Abdul Khair ◽  
Noraida Noraida
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Hemorrhagic Fever ◽  
Control Group ◽  
Leaf Extracts ◽  
Control Group Design ◽  
Vector Borne Diseases ◽  
Apple Leaves ◽  
Significant Difference ◽  
Vector Borne

Vector-borne diseases are still a health problem in some tropical countries. One vector-borne disease is Dengue Hemorrhagic Fever (DHF). DHF never decreases and even tends to continue to increase, and many cause deaths in children, 90% of them attack children under 15 years. DHF cases in 2018 amounted to 65,602 cases, with 467 deaths (CFR = 0.71%). The behavior of holding water in various places such as tubs and tendons indirectly creates a breeding place for Aedes aegypti mosquitoes. The use of chemicals as insecticides can cause the death of non-target animals, environmental pollution, and the occurrence of vector resistance to insecticides. Therefore it is necessary to do other methods including the use of vegetable insecticides. Sugar apple leaves can be used to kill Aedes aegypti larvae. Sugar apple leaves will obtain throughout the year. The purpose of this study is to know the ability of young and old sugar apple leaves to kill Aedes aegypti larvae. This type of research is a posttest only control group design. The study sample was part of the Aedes aegypti larvae on the final instar III. The experiment to kill Aedes aegypti larvae use two types of sugar apple leaf extract, namely young and old sugar apple leaves with nine treatments four replications. Each treatment consisted of 25 larvae. The research results show There was no significant difference in the number of dead larvae using either extracts from young sugar apple leaves or old sugar apple leaves. LC90 of sugar apple leaf extract was between 0.05632 to 0.08324% and the effective residual age at LC90 (0.06568%) with the death of Aedes aegypti larvae was 92% over 24 hours (1 day).

scholarly journals Short communication: New report of Aedes albopictus in Souk Ahras, Northeast Algeria

2021 ◽  
Vol 22 (7) ◽  
Author(s):  
Kaouther Hamaidia ◽  
Noreddine Soltani
Keyword(s):  
Aedes Albopictus ◽  
Short Communication ◽  
Asian Tiger Mosquito ◽  
Breeding Sites ◽  
Vector Borne Diseases ◽  
Asian Tiger ◽  
Mosquito Breeding ◽  
Vector Borne ◽  
The City ◽  
Residential Garden

Abstract. Hamaidia K, Soltani N. 2021. Short communication: New report of Aedes albopictus in Souk Ahras, Northeast Algeria. Biodiversitas 22: 2901-2906. The present paper reports the occurrence of Aedes albopictus (Skuse), an aggressive Asian tiger mosquito in the city of Souk-Ahras (Northeast of Algeria). A “26 April” estate’ inhabitants (Souk-Ahras province) have reported unusual daytime bites by a striped mosquito. On early September 2020, an intensive field inspection was carried out for potential mosquito breeding sites in the locations around the city. A total of 105 specimens (8 larvae, 24 pupae and 73 adults) of Ae. albopictus were collected in a pile of old tyres in a residential garden. After previous records of this species in Algeria, this is the first evidence of its presence in Souk-Ahras province, and the findings enhance combined public participation with professional validation in surveillance of vector borne-diseases programs with emphasis on the need for sensitising citizens about controlling this important vector.

scholarly journals ENTOMOLOGICAL PARAMETERS AND CHARACTERIZATION OF INSECTICIDE RESISTANCE IN DENGUE VECTOR AEDES AEGYPTI LARVAE FROM BENGKULU CITY, INDONESIA

2021 ◽  
Vol 21 (1) ◽  
pp. 96-102
Author(s):  
Dessy Triana ◽  
Fitaloca N Siregar ◽  
Eka Purnama Wulan Tri Utami ◽  
Tatik Suteky ◽  
Swandito Wicaksono
Keyword(s):  
Aedes Aegypti ◽  
Cross Sectional ◽  
Vector Borne Diseases ◽  
Control Programs ◽  
Vector Population ◽  
Who 1999 Criteria ◽  
Vector Borne ◽  
Dengue Control ◽  
Breeding Place

In Bengkulu, as in other areas of Indonesia, dengue is one of the most important vector-borne diseases. The primary strategy to control dengue is by reducing the vector population using insecticides. However, applications of insecticides becomes a contributing factor in the development of vector resistance. As there is no effective dengue drug or safe vaccine available, vector control remains the most effective intervention to reduce incidence and prevent transmission and outbreak. This study aimed to determine the entomological parameters and resistance status to temephos of Aedes aegypti larvae from Lingkar Barat village and Jalan Gedang village (dengue endemic areas). This study used cross-sectional and experimental with post-control only design. The sampling refers to the guidelines for dengue entomology surveys according to WHO 1999 criteria. Aedes aegypti eggs were collected by ovitraps and reared to larvae stage. Susceptibility test of larvae to the temephos was estimated using standard WHO. Mortality of larvae Ae. aegypti after 24 hours with multiple concentration of temephos indicative of highly resistant.  Entomological parameters for house index (HI), container index (CI) and container pupa index (CPI) in Jalan Gedang village and Lingkar Barat village were estimated: 7%, 12% for HI and 1.97%, 3.43% for CI, and 5%, 2% for CPI, respectively. The proportion of controllable sites is higher than disposable sites, it has a high risk as breeding places for mosquitoes indicates a high potential risk as breeding place and transmission of dengue. Awareness sanitation in the home and surrounding environment must be possessed by each individual community in order to prevent and overcome the incidence of dengue. These data should be of value in formulating dengue control programs.

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