scholarly journals De novo transcriptome sequencing of Serangium japonicum (Coleoptera: Coccinellidae) and application of two assembled unigenes

2019 ◽  
Author(s):  
Ya Hui Hu ◽  
Yong Liu ◽  
Lin Wei ◽  
Hao Tao Chen
Keyword(s):  
Transcriptome Sequencing ◽  
De Novo ◽  
Molecular Data ◽  
Gene Sequences ◽  
De Novo Transcriptome ◽  
Ladybird Beetle ◽  
Important Predator ◽  
Serangium Japonicum ◽  
Molecular Barcode ◽  
Paired End Sequencing

AbstractThe ladybird beetle Serangium japonicum is an important predator of whiteflies. Although some ecological and biological characteristics of this predator have been studied, the only molecular data for the ladybird beetle at the NCBI website has been submitted by us. To yield gene sequences of the ladybird beetle, paired-end sequencing was used to sequence its transcriptome. Real-time PCR was used to validate differences in the quantity of RNA expressed by the Krueppel homolog 1 (Kr-h1) gene in summer versus winter samples. To find the effective molecular barcode, the annotated cytochrome c oxidase subunit I (COX1) gene fragments were amplified from several ladybird beetle populations. Analysis yielded 191,246 assembled unigenes, of which 127,016 (66.4%) were annotated. The differential expression of Kr-h1 in summer versus winter suggests that S. japonicum can successfully overwinter because the adults enter diapause. The annotated COX1 gene can be used to distinguish S. japonicum from other ladybird species. These gene sequences are currently available in the National Center for Biotechnology Information (NCBI), and will facilitate the study of molecular mechanism in S. japonicum.

scholarly journals De Novo Transcriptome Sequencing of Serangium japonicum (Coleoptera: Coccinellidae) and Application of Two Assembled Unigenes

2019 ◽  
Vol 10 (1) ◽  
pp. 247-254
Author(s):  
Ya Hui Hu ◽  
Yong Liu ◽  
Lin Wei ◽  
Hao Tao Chen
Keyword(s):  
Gene Expression ◽  
Molecular Mechanisms ◽  
De Novo ◽  
Sequence Data ◽  
Gene Sequences ◽  
Methyl Farnesoate ◽  
Ladybird Beetle ◽  
Functional Annotations ◽  
Serangium Japonicum ◽  
Paired End Sequencing

The ladybird beetle Serangium japonicum is an important predator of whiteflies. Investigations of the molecular mechanisms of this predatory beetle have been hindered by the scarcity of gene sequence data. To obtain gene sequences for the ladybird beetle and determine differences in gene expression between the summer and winter seasons, paired-end sequencing was performed. Real-time PCR was used to validate differences in Krueppel homolog 1 gene (Kr-h1) mRNA expression in summer vs. winter samples. To determined the diversity of the population, annotated cytochrome c oxidase subunit I gene (COX1) gene fragments were amplified from several ladybird beetle populations. The analysis yielded 191,246 assembled unigenes, 127,016 of which (66.4%) were annotated. These functional annotations of gene sequences are currently available from the National Center for Biotechnology Information (NCBI), and will provide a basis for studying the molecular mechanisms underlying the biological characteristics of S. japonicum. We found a change in expression of ribosome-associated genes across seasons, and postulate that this change is because of seasonal variation in temperature and photoperiod. The differential expression of Kr-h1 suggests that S. japonicum can successfully overwinter because the adults enter diapause. To explain the effects of season on Kr-h1 gene expression, we hypothesize a model in which that a short photoperiod affects the density of Ca2+, the subsequent activity of methyl farnesoate epoxidase and the synthesis of JH, and in turn Kr-h1 gene expression. COX1 annotation was concordant with the morphological ID. The same COX1 sequence was found in the samples from several provinces in China. Therefore, the COX1 sequence is worth further study to distinguish beetle species and populations.

De novo transcriptome sequencing of a non-model polychaete species

2016 ◽  
Vol 29 ◽  
pp. 31-34 ◽  
Author(s):  
E. Cannarsa ◽  
G. Zampicinini ◽  
O. Friard ◽  
A. Santovito ◽  
P. Cervella
Keyword(s):  
Transcriptome Sequencing ◽  
De Novo ◽  
De Novo Transcriptome ◽  
Polychaete Species ◽  
De Novo Transcriptome Sequencing

De novo transcriptome sequencing and identification of genes related to salt and PEG stress in Tetraena mongolica Maxim

Trees ◽  
2019 ◽  
Vol 33 (6) ◽  
pp. 1639-1656 ◽  
Author(s):  
Ningmei Chen ◽  
Jinchao Feng ◽  
Buerbatu Song ◽  
Shuai Tang ◽  
Junqing He ◽  
...  
Keyword(s):  
Transcriptome Sequencing ◽  
De Novo ◽  
De Novo Transcriptome ◽  
De Novo Transcriptome Sequencing ◽  
Peg Stress
Sign in / Sign up

Export Citation Format

Share Document