scholarly journals Ligands, receptors and transcription factors that mediate inter-cellular and intra-cellular communication during ovarian follicle development

2019 ◽  
Author(s):  
Beatriz Peñalver Bernabé ◽  
Teresa Woodruff ◽  
Linda J Broadbelt ◽  
Lonnie D Shea
Keyword(s):  
Transcription Factors ◽  
Ovarian Follicle ◽  
Somatic Cells ◽  
Follicle Development ◽  
Paracrine Signaling ◽  
Oocyte Growth ◽  
Ovarian Follicle Development ◽  
Ovarian Folliculogenesis ◽  
Follicle Maturation

SUMMARYReliably producing a competent oocyte entails a deeper comprehension of ovarian follicle maturation, a very complex process that includes meiotic maturation of the female gamete, the oocyte, together with the mitotic divisions of the hormone-producing somatic cells. In this report, we investigate mice ovarian folliculogenesis in vivo using publically available time-series microarrays from primordial to antral stage follicles. Manually curated protein interaction networks were employed to identify autocrine and paracrine signaling between the oocyte and the somatic cells (granulosa and theca cells) and the oocyte and cumulus and mural cells at multiple stages of follicle development. We established protein binding interactions between expressed genes that encoded secreted factors and expressed genes that encoded cellular receptors. Some of computationally identified signaling interactions are well established, such as the paracrine signaling from the oocyte to the somatic cells through the secreted oocyte growth factor Gdf9; while others are novel connections in term of ovarian folliculogenesis, such as the possible paracrine connection from somatic secreted factor Ntn3 to the oocyte receptor Neo1. Additionally, we identify several of the likely transcription factors that might control the dynamic transcriptome during ovarian follicle development, noting that the YAP/TAP signaling is very active in vivo. This novel dynamic model of signaling and regulation can be employed to generate testable hypotheses regarding follicle development, guide the improvement of culture media to enhance in vitro ovarian follicle maturation and possibly as novel therapeutic targets for reproductive diseases.

scholarly journals Correction to: Synergy of Paracrine Signaling During Early-Stage Mouse Ovarian Follicle Development In Vitro

2018 ◽  
Vol 11 (6) ◽  
pp. 537-537
Author(s):  
Hong Zhou ◽  
Joseph T. Decker ◽  
Melissa M. Lemke ◽  
Claire E. Tomaszewski ◽  
Lonnie D. Shea ◽  
...  
Keyword(s):  
Early Stage ◽  
Ovarian Follicle ◽  
Follicle Development ◽  
Paracrine Signaling ◽  
Ovarian Follicle Development ◽  
Development In Vitro

scholarly journals Synergy of Paracrine Signaling During Early-Stage Mouse Ovarian Follicle Development In Vitro

2018 ◽  
Vol 11 (5) ◽  
pp. 435-450 ◽  
Author(s):  
Hong Zhou ◽  
Joseph T. Decker ◽  
Melissa M. Lemke ◽  
Claire E. Tomaszweski ◽  
Lonnie D. Shea ◽  
...  
Keyword(s):  
Early Stage ◽  
Ovarian Follicle ◽  
Follicle Development ◽  
Paracrine Signaling ◽  
Ovarian Follicle Development ◽  
Development In Vitro

scholarly journals Ablation of TGFBR3 (betaglycan) in oocytes does not affect fertility in female mice

Reproduction ◽  
2020 ◽  
Author(s):  
Yining Li ◽  
Yeu-Farn Lin ◽  
Xiang Zhou ◽  
Hugh J Clarke ◽  
Daniel J. Bernard
Keyword(s):  
Protein Expression ◽  
Ovarian Follicle ◽  
Cell Types ◽  
Follicle Development ◽  
Cell Type ◽  
Ovarian Cell ◽  
Functional Roles ◽  
Ovarian Follicle Development ◽  
Tgfβ Superfamily

Ovarian follicle development is regulated by locally produced TGFβ superfamily members. The TGFβ type III receptor (TGFBR3, or betaglycan), which regulates the actions of diverse TGFβ ligands, including inhibins, is expressed in different ovarian cell types. However, its functional roles in the ovary have not been investigated in vivo. Here, we ablated Tgfbr3 in murine oocytes using the Cre-loxP system. Oocyte-specific Tgfbr3 knockout (cKO) females were fertile, producing litters of similar size and frequency as controls. Their ovarian weights and histology were also normal. Though we confirmed efficient recombination of the floxed alleles, we did not detect Tgfbr3 mRNA in purified oocytes from superovulated cKO or control mice. These results challenge earlier observations of betaglycan protein expression in this cell type. Regardless, Tgfbr3 in the murine oocyte is clearly dispensable for female fertility.

scholarly journals Initial and Cyclic Recruitment of Ovarian Follicles*

2000 ◽  
Vol 21 (2) ◽  
pp. 200-214 ◽  
Author(s):  
Elizabeth A. McGee ◽  
Aaron J. W. Hsueh
Keyword(s):  
Ovarian Follicle ◽  
Ovarian Follicles ◽  
Follicle Development ◽  
Ovarian Follicle Development ◽  
Branching Points ◽  
Reproductive Techniques ◽  
Recruitment And Selection ◽  
Cyclic Recruitment

Abstract Mammalian ovaries consist of follicles as basic functional units. The total number of ovarian follicles is determined early in life, and the depletion of this pool leads to reproductive senescence. Each follicle develops to either ovulate or, more likely, to undergo degeneration. The dynamics of ovarian follicle development have interested endocrinologists and developmental biologists for many years. With the advent of assisted reproductive techniques in humans, the possibility of regulating follicle development in vivo and in vitro has gained clinical relevance. In this review, we focus upon key branching points during the development of ovarian follicles as well as factors involved in determining the eventual destiny of individual follicles. We discuss inconsistencies in the literature regarding the definitions of follicle recruitment and selection and propose to name the two major steps of follicle development as initial and cyclic recruitment, respectively. Because some of these disparities have arisen due to differences in the animal systems studied, we also compare the development of the ovarian follicles of both humans and rats. We also review the status of knowledge of several puzzling clinical issues that may provide important clues toward unlocking the mechanisms of follicle development.

Effects of active immunization against inhibin and androstenedione, separately or together, on hormone profiles, ovarian follicle populations and ovulation rate in heifers

1991 ◽  
Vol 1991 ◽  
pp. 17-17
Author(s):  
S.M. Rhind ◽  
S.R. Schemm ◽  
B.D. Schanbacher
Keyword(s):  
Ovarian Follicle ◽  
Pulse Frequency ◽  
Active Immunization ◽  
Follicular Phase ◽  
Ovulation Rate ◽  
Follicle Development ◽  
Ovarian Follicle Development ◽  
Endocrine Status ◽  
Ovulatory Follicles ◽  
Follicle Maturation

It was postulated that the induction of twin ovulations in cattle may require a combination of two appropriate stimuli. The first putative requirement is an increase in the circulating FSH concentrations and/or a decrease in inhibin concentrations to induce the development of additional potentially ovulatory follicles. The second putative requirement is an increase in LH pulse frequency during the follicular phase of the cycle to enhance the stimulus for follicle maturation and ovulation. On the basis of studies reported in the literature, it was suggested that such stimuli may be provided by immunization against inhibin and against a steroid hormone such as androstenedione, respectively. This experiment was designed to determine the effects of immunization against androstenedione and inhibin, either separately or together on the endocrine status of heifers and on the associated patterns of ovarian follicle development and ovulation rate.

scholarly journals Cellular basis for paracrine regulation of ovarian follicle development

Reproduction ◽  
2001 ◽  
pp. 647-653 ◽  
Author(s):  
DF Albertini ◽  
CM Combelles ◽  
E Benecchi ◽  
MJ Carabatsos
Keyword(s):  
Granulosa Cells ◽  
Assisted Reproductive Technologies ◽  
Developmental Regulation ◽  
Ovarian Follicle ◽  
Somatic Cells ◽  
Reproductive Technologies ◽  
Follicle Development ◽  
Paracrine Factors ◽  
Primordial Follicles ◽  
Ovarian Follicle Development

Paracrine factors secreted by oocytes and somatic cells regulate many important aspects of early ovarian follicle development in mammals. From activation of dormant primordial follicles to selection of secondary follicles, locally acting factors have been identified that appear to exert important effects on the growth and differentiation of oocytes and granulosa cells. This article summarizes evidence to support a model for bi-directional paracrine communication that is based on developmental regulation of the delivery and reception of paracrine factors at the oocyte-granulosa cell interface. Transzonal projections that originate from granulosa cells and terminate at the oocyte plasma membrane provide a polarized means to orient the secretory organelles of somatic cells. Characterization of transzonal projections in follicles from normal and genetically modified mice reveals dynamic changes in the density and stability of transzonal projections. On the basis of new data analysing the orientation and cytoskeletal content of transzonal projections in mammalian oocytes, a model is proposed for regulation of paracrine growth factor secretion by follicle-stimulating hormone. These findings have immediate implications for ovarian hyperstimulation protocols and follicle culture models as related to the production of mammalian embryos by assisted reproductive technologies.

scholarly journals Granulosa cell genes that regulate ovarian follicle development beyond the antral stage: the role of estrogen receptor β

2020 ◽  
Author(s):  
V. Praveen Chakravarthi ◽  
Shaon Borosha ◽  
Subhra Ghosh ◽  
Katherine F. Roby ◽  
Michael W. Wolfe ◽  
...  
Keyword(s):  
Target Genes ◽  
Ovarian Follicle ◽  
Female Rats ◽  
Spatiotemporal Pattern ◽  
Follicle Development ◽  
Ovarian Follicle Development ◽  
Gonadotropin Stimulation ◽  
Before And After ◽  
Induced Genes ◽  
Follicle Maturation

ABSTRACTFollicle development beyond the preantral stage is dependent on gonadotropins. FSH signaling is crucial for the advancement of preantral follicles to the antral stage, and LH signaling is essential for further maturation of preovulatory follicles. Estrogen is intricately tied to gonadotropin signaling during the advanced stages of folliculogenesis. We observed that Erβnull ovarian follicles fail to develop beyond the antral stage, even after exogenous gonadotropin stimulation. As ERβ is primarily expressed in the granulosa cells (GCs), we explored the gonadotropin-regulated GC genes that induce maturation of antral follicles. Synchronized follicle development was induced by administration of exogenous gonadotropins to wildtype 4-wk-old female rats. The GC transcriptome was analyzed via RNA-sequencing before and after gonadotropin stimulation. An Erβnull mutant model that fails to show follicle maturation was also included in order to identify the ERβ-regulated genes involved at this step. We observed that specific groups of genes were differentially expressed in response to PMSG or hCG administration in wildtype rats. While some of the PMSG or hCG-induced genes showed a similar expression pattern in Erβnull GCs, a subset of PMSG- or hCG-induced genes showed a differential expression in Erβnull GCs. These latter ERβ-regulated genes included previously known FSH or LH target genes including Lhcgr, Cyp11a1, Cyp19a1, Pgr, Runx2, Egfr, Kiss1, and Ptgs2, which are involved in follicle development, oocyte maturation, and ovulation. We also identified novel ERβ-regulated genes including Jaml, Galnt6, Znf750, Dusp9, Wnt16, and Mageb16 that failed to respond to gonadotropin stimulation in Erβnull GCs. Our findings indicate that the gonadotropin-induced spatiotemporal pattern of gene expression is essential for ovarian follicle maturation beyond the antral stage. However, expression of a subset of those gonadotropin-induced genes is dependent on transcriptional regulation by ERβ.

scholarly journals Anti-Müllerian Hormone Attenuates the Effects of FSH on Follicle Development in the Mouse Ovary

Endocrinology ◽  
2001 ◽  
Vol 142 (11) ◽  
pp. 4891-4899 ◽  
Author(s):  
Alexandra L. L. Durlinger ◽  
Maria J. G. Gruijters ◽  
Piet Kramer ◽  
Bas Karels ◽  
T. Rajendra Kumar ◽  
...  
Keyword(s):  
Ovarian Follicle ◽  
Inhibitory Effect ◽  
Ovarian Follicles ◽  
Follicle Development ◽  
Wild Type ◽  
Follicle Growth ◽  
Primordial Follicles ◽  
Ovarian Follicle Development

Abstract Although ovarian follicle growth is under the influence of many growth factors and hormones of which FSH remains one of the most prominent regulators. Therefore, factors affecting the sensitivity of ovarian follicles to FSH are also important for follicle growth. The aim of the present study was to investigate whether anti-Müllerian hormone (AMH) has an inhibitory effect on follicle growth by decreasing the sensitivity of ovarian follicles to FSH. Furthermore, the combined action of AMH and FSH on ovarian follicle development was examined. Three different experiments were performed. Using an in vitro follicle culture system it was shown that FSH-stimulated preantral follicle growth is attenuated in the presence of AMH. This observation was confirmed by an in vivo experiment showing that in immature AMH-deficient females, more follicles start to grow under the influence of exogenous FSH than in their wild-type littermates. In a third experiment, examination of the follicle population of 4-month-old wild-type, FSHβ-, AMH-, and AMH-/FSHβ-deficient females revealed that loss of FSH expression has no impact on the number of primordial and preantral follicles, but the loss of inhibitory action of AMH on the recruitment of primordial follicles in AMH-deficient mice is increased in the absence of FSH. In conclusion, these studies show that AMH inhibits FSH-stimulated follicle growth in the mouse, suggesting that AMH is one of the factors determining the sensitivity of ovarian follicles for FSH and that AMH is a dominant regulator of early follicle growth.

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