scholarly journals Rational nanotoolbox with theranostic potential for medicated pro-regenerative corneal implants

2019 ◽  
Author(s):  
Hirak K. Patra ◽  
Mohammad Azharuddin ◽  
Mohammad M. Islam ◽  
Georgia Papapavlou ◽  
Suryyani Deb ◽  
...  
Keyword(s):  
Disease Burden ◽  
Unmet Need ◽  
Host Environment ◽  
Over Expression ◽  
Epithelial Regeneration ◽  
Non Invasive ◽  
Artificial Corneas ◽  
Successful Transplantation ◽  
Implant Degradation

AbstractCornea diseases are a leading cause of blindness and the disease burden is exacerbated by the increasing shortage around the world for cadaveric donor corneas. Despite the advances in the field of regenerative medicine, successful transplantation of laboratory made artificial corneas has not been fully realised in clinical practice. The causes of failure of such artificial corneal implants are multifactorial and include latent infections from viruses and other micorbes, enzyme over-expression, implant degradation, extrusion or delayed epithelial regeneration. Therefore, there is an urgent unmet need for developing customized corneal implants to suit the host environment, counter the effects of inflammation or infection and that are able to track early signs of implant failurein situ. In the present work, we describe a nano toolbox comprising tools for drug release and in addition capable of being infection responsive, promoting regeneration including non-invasive monitoring ofin situcorneal environment. These nano constructs can be incorporated within pro-regenerative biosynthetic implants, transforming them into theranostic devices able to respond to biological changes following implantation.

Efficiency of diagnosis and treatment non-invasive and microinvasive sgamous cell cervical cancer

2020 ◽  
pp. 71-74
Author(s):  
M.M. Melnyk ◽  
◽  
S.V. Nespradko ◽  
I.V. Goncharuk ◽  
M.V. Marchenko ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Key Words ◽  
National Cancer Institute ◽  
Reproductive Function ◽  
Diagnosis And Treatment ◽  
Non Invasive ◽  
Corpus Uteri ◽  
Complex Program ◽  
Early Cervical Cancer

The objective: analyse the effectiveness of diagnosis and treatment for early cervical cancer. Materials and methods. Analysed 107 cases of women’s disease on CIN ІІІ, cancer in situ, they were on treatment in National cancer institute and Kyiv dictrict cancer dispensary from 2010 till 2015 years. Results. Diagnosed percent relapse CIN ІІІ, cancer in situ contain 4.57% uninvasive and invasive form – 0.94%. Conclusion. According diagnostic CIN ІІ and CIN ІІІ is recommended to do treatment conization and dynamic dispensary observation. Are making complex program of infection HPV16, 18. In appering of margins resection some elements of tumor after wider conization by forms of cancer in situ. Many of expansive burns in cervical glands, in making of reproductive function, going disease (nodel leiomyoma of corpus uteri etc). In perspective is accept the notion of looking after and screening research of considering infection HPV16, 18 on CIN І, CIN ІІ. Key words: cervical cancer, сancer in situ, CIN І–ІІІ, diagnostic, treatment, conization.

scholarly journals Prognostic and Clinicopathological Significance of MiR-155 in Breast Cancer: A Systematic Review

2020 ◽  
Vol 21 (16) ◽  
pp. 5834
Author(s):  
Anna Maria Grimaldi ◽  
Silvia Nuzzo ◽  
Gerolama Condorelli ◽  
Marco Salvatore ◽  
Mariarosaria Incoronato
Keyword(s):  
Breast Cancer ◽  
Systematic Review ◽  
Prognostic Biomarker ◽  
Survival Rates ◽  
Prognostic Significance ◽  
Unmet Need ◽  
Non Invasive ◽  
Clinicopathological Significance ◽  
Present Systematic Review

There is an unmet need for novel non-invasive prognostic molecular tumour markers for breast cancer (BC). Accumulating evidence shows that miR-155 plays a pivotal role in tumorigenesis. Generally, miR-155 is considered an oncogenic miRNA promoting tumour growth, angiogenesis and aggressiveness of BC. Therefore, many researchers have focused on its use as a prognostic biomarker and therapeutic target. However, its prognostic value for BC patients remains controversial. To address this issue, the present systematic review aims to summarize the available evidence and give a picture of a prognostic significance of miR-155 in BC pathology. All eligible studies were searched on PubMed and EMBASE databases through various search strategies. Starting from 289 potential eligible records, data were examined from 28 studies, comparing tissue and circulating miR-155 expression levels with clinicopathological features and survival rates in BC patients. We discuss the pitfalls and challenges that need to be assessed to understand the power of miR-155 to respond to real clinical needs, highlighting the consistency, robustness or lack of results obtained to sate in translating this molecule to clinical practice. Our paper suggests that the prognostic role of miR-155 in the management of BC needs to be further verified.

scholarly journals Proteins and microRNAs are differentially expressed in tear fluid from patients with Alzheimer’s disease

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Aidan Kenny ◽  
Eva M. Jiménez-Mateos ◽  
María Ascensión Zea-Sevilla ◽  
Alberto Rábano ◽  
Pablo Gili-Manzanaro ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Unmet Need ◽  
Cost Effective ◽  
Specific Protein ◽  
Tear Fluid ◽  
Initiation Factor ◽  
Non Invasive ◽  
A Genome ◽  
Potential Biomarker

Abstract Alzheimer’s disease (AD) is characterized by a progressive loss of neurons and cognitive functions. Therefore, early diagnosis of AD is critical. The development of practical and non-invasive diagnostic tests for AD remains, however, an unmet need. In the present proof-of-concept study we investigated tear fluid as a novel source of disease-specific protein and microRNA-based biomarkers for AD development using samples from patients with mild cognitive impairment (MCI) and AD. Tear protein content was evaluated via liquid chromatography-mass spectrometry and microRNA content was profiled using a genome-wide high-throughput PCR-based platform. These complementary approaches identified enrichment of specific proteins and microRNAs in tear fluid of AD patients. In particular, we identified elongation initiation factor 4E (eIF4E) as a unique protein present only in AD samples. Total microRNA abundance was found to be higher in tears from AD patients. Among individual microRNAs, microRNA-200b-5p was identified as a potential biomarker for AD with elevated levels present in AD tear fluid samples compared to controls. Our study suggests that tears may be a useful novel source of biomarkers for AD and that the identification and verification of biomarkers within tears may allow for the development of a non-invasive and cost-effective diagnostic test for AD.

scholarly journals Optical nanomanipulation on solid substrates via optothermally-gated photon nudging

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jingang Li ◽  
Yaoran Liu ◽  
Linhan Lin ◽  
Mingsong Wang ◽  
Taizhi Jiang ◽  
...  
Keyword(s):  
Colloidal Particles ◽  
Solid Substrate ◽  
Optical Scattering ◽  
Functional Nanostructures ◽  
Non Invasive ◽  
Solid Substrates ◽  
All Optical ◽  
Scattering Force ◽  
Reconfigurable Assembly

AbstractConstructing colloidal particles into functional nanostructures, materials, and devices is a promising yet challenging direction. Many optical techniques have been developed to trap, manipulate, assemble, and print colloidal particles from aqueous solutions into desired configurations on solid substrates. However, these techniques operated in liquid environments generally suffer from pattern collapses, Brownian motion, and challenges that come with reconfigurable assembly. Here, we develop an all-optical technique, termed optothermally-gated photon nudging (OPN), for the versatile manipulation and dynamic patterning of a variety of colloidal particles on a solid substrate at nanoscale accuracy. OPN takes advantage of a thin surfactant layer to optothermally modulate the particle-substrate interaction, which enables the manipulation of colloidal particles on solid substrates with optical scattering force. Along with in situ optical spectroscopy, our non-invasive and contactless nanomanipulation technique will find various applications in nanofabrication, nanophotonics, nanoelectronics, and colloidal sciences.

Non-invasive in-situ analysis of a wreath of gold leaves from the National Archaeological Museum of Taranto, Italy

Measurement ◽  
2018 ◽  
Vol 126 ◽  
pp. 164-167 ◽  
Author(s):  
Alessandro Buccolieri ◽  
Eva Degl'Innocenti ◽  
Roberto Cesareo ◽  
Alfredo Castellano ◽  
Giovanni Buccolieri
Keyword(s):  
In Situ Analysis ◽  
Non Invasive

Żelazna Nowa, stanowisko 2. Cmentarzysko kultury przeworskiej z Zapilcza na południowym Mazowszu

2020 ◽  
Author(s):  
Małgorzata Cieślak-Kopyt ◽  
Dorota Pogodzińska
Keyword(s):  
Material Culture ◽  
Archaeological Heritage ◽  
Non Invasive ◽  
Vistula River ◽  
Animal Bones ◽  
The Subject ◽  
Metal Detectors ◽  
Przeworsk Culture ◽  
Small Finds

The subject of the monograph, published as the 10th volume of the Saved Archaeological Heritage series, are the results of rescue excavations on a cemetery from the period of Roman influence on the Vistula River near Magnuszew in southern Mazovia (Poland), carried out several years ago at the initiative of the Museum in Radom. This necropolis, like many similar ones throughout the country, was systematically destroyed as a result of agricultural activities, and in recent years also through illegal prospection with the use of metal detectors. Archaeologists, with the cooperation of numerous volunteers, managed to protect against further destruction about 60 graves (urned and urnless) from the period between the 1st century BC and the 3rd century CE. These are an evidence of the settlement of the region by people whose material traces are referred to in the archaeological nomenclature as the Przeworsk culture (associated mainly with the Germanic tribes). The cinerary graves were equipped with ceramics, metal parts of clothing, tools, less often weapons, glass beads, imported vessels or dice. Among the forms of graves, the so-called groove object stands out: a kind of rectangular grave feature tied with survival to the beginnings of our era of Celtic traditions, arriving here from northern Małopolska. In addition to the standard catalogue with the description of graves, pottery and small finds, and very detailed illustration plates, the monograph includes an analysis of material culture and forms of burial, photographs of selected finds and very extensive specialist reports. The latter include both osteological materials (anatomo-anthropological analysis, analysis of animal bones placed in the graves), as well as other ecofacts and individual categories of furnishings (glass, faience, iron and bronze objects). The whole is complemented by clear plans with the location of graves and artifacts in the necropolises, as well as with the results of non-invasive research going far beyond the excavated area and of key importance for further in situ protection of this extremely valuable monument.

scholarly journals Engaging with and Shaping Nature: A Nature-Based Intervention for Those with Mental Health and Behavioural Problems at the Westonbirt Arboretum in England

2018 ◽  
Vol 15 (10) ◽  
pp. 2214 ◽  
Author(s):  
Liz O’Brien
Keyword(s):  
Mental Health ◽  
Disease Burden ◽  
Mental Health Problems ◽  
Well Being ◽  
Behavioural Problems ◽  
Supportive Environment ◽  
Wide Range ◽  
Causes Of Disease ◽  
Repeat Visits

Mental health problems have become one of the leading causes of disease burden worldwide. This study used qualitative mixed methods including in-situ ‘being and doing’ activities with participants, interviews, and participant observations to explore participant’s experiences of a multi-visit nature-based intervention at Westonbirt Arboretum in England. The research found that three engagement types: (1) social, (2) woodland craft, and (3) creative and sensory, provided a meaningful programme to engage those with mental health, addiction, autism and behavioural problems. These types of engagement conferred a wide range of well-being benefits on participants. The study highlights key elements of the programme that were effective including the importance of repeat visits to nature to enable familiarity, using creative, sensory and craft activities, creating a supportive environment, involving the volunteers, and understanding the needs of participants and the organisations that work with them. The research suggests that nature-based programmes can be designed to be flexible and adaptable to meet the needs of participants with mental health and behavioural problems. Small numbers of participants can be involved in an intensive and immersive way that encourages an emotional affinity with nature. Inclusive and supportive programmes are particularly important for those who are vulnerable, as they are less likely to engage with nature than the wider population.

scholarly journals Expression of Spred2 in the urothelial tumorigenesis of the urinary bladder

2021 ◽  
Author(s):  
Shinsuke Oda ◽  
Masayoshi Fujisawa ◽  
Li Chunning ◽  
Toshihiro Ito ◽  
Takahiro Yamaguchi ◽  
...  
Keyword(s):  
Urothelial Carcinoma ◽  
Cancer Progression ◽  
Mapk Pathway ◽  
Negative Regulator ◽  
Mitogen Activated Protein Kinase ◽  
Ki67 Index ◽  
Erk Activation ◽  
Non Invasive ◽  
Erk Mapk

Aberrant activation of the Ras/Raf/ERK (extracellular-signal-regulated kinase)-MAPK (mitogen-activated protein kinase) pathway is involved in the progression of cancer, including urothelial carcinoma; but the negative regulation remains unclear. In the present study, we investigated pathological expression of Spred2 (Sprouty-related EVH1 domain-containing protein 2), a negative regulator of the Ras/Raf/ERK-MAPK pathway, and the relation to ERK activation and Ki67 index in various categories of 275 urothelial tumors obtained from clinical patients. In situ hybridization demonstrated that Spred2 mRNA was highly expressed in high-grade non-invasive papillary urothelial carcinoma (HGPUC), and the expression was decreased in carcinoma in situ (CIS) and infiltrating urothelial carcinoma (IUC). Immunohistochemically, membranous Spred2 expression, important to interact with Ras/Raf, was preferentially found in HGPUC. Interestingly, membranous Spred2 expression was decreased in CIS and IUC relative to HGPUC, while ERK activation and the expression of the cell proliferation marker Ki67 index were increased. HGPUC with membranous Spred2 expression correlated significantly with lower levels of ERK activation and Ki67 index as compared to those with negative Spred2 expression. Thus, our pathological findings suggest that Spred2 negatively regulates cancer progression in non-invasive papillary carcinoma possibly through inhibiting the Ras/Raf/ERK-MAPK pathway, but this regulatory mechanism is lost in cancers with high malignancy. Spred2 appears to be a key regulator in the progression of non-invasive bladder carcinoma.

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