scholarly journals Selection of a Malignant Subpopulation from a Colorectal Cancer Cell Line

2019 ◽  
Author(s):  
Pei-Lun Lai ◽  
Ting-Chun Chen ◽  
Chun-Yen Feng ◽  
Hsuan Lin ◽  
Ng Wu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Prognostic Biomarker ◽  
Differentially Expressed Gene ◽  
Cancer Cell Line ◽  
Experimental Models ◽  
Colorectal Cancer Cell Line ◽  
Selection Of

AbstractColorectal cancer (CRC) is a leading cause of death from cancer worldwide. Thus, there is an emerging need for new experimental models that allow identification and validation of biomarkers for CRC-specific progression. In this study, we propose a repeated sphere-forming assay as a strategy to select a malignant subpopulation from a CRC line, HCT116. We validated our assay by confirming that three canonical stemness markers, Nanog, Oct4, and Lgr5, were up-regulated in the sphere state at every generation of the selection assay. The resulting line, after eight rounds of selection, exhibited an increased sphere-forming capacityin vitroand tumorgenicityin vivo. Furthermore, dipeptidase 1 (DPEP1) was identified as the major differentially expressed gene in the selected clone, and depletion of DPEP1 suppressed the elevated sphere-forming capacityin vitroand tumorgenicityin vivo. Overall, we have established an experimental strategy for the isolation of a malignant subpopulation from a CRC cell line. Results from our model also suggested that DPEP1 can serve as a promising prognostic biomarker for CRC.

scholarly journals Antiproliferative Effect of Aspirin on Colorectal Cancer Cell Line

2018 ◽  
Vol 12 (5) ◽  
pp. 1-4
Author(s):  
Malihe Bagheri ◽  
◽  
Amir Reza Hesari ◽  
Parisa Zia Sarabi ◽  
Hamid Reza Rahimi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell Line ◽  
Sw480 Cell ◽  
Colorectal Cancer Cell Line ◽  
Range Analysis ◽  
Cytotoxic Effects

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) such as Aspirin may have anticancer properties, and can be effective as a novel strategy for the treatment of colorectal cancer (CRC). The aim of this study was to assess the cytotoxic effects of Aspirin drug in CRC cell lines compared with Oxaliplatin drug in vitro. Methods: Cell viability was assessed after treatment of SW742 and SW480 cells with Aspirin and Oxaliplatin by MTT assay, and the amount of IC50 was determined. Statistical analysis was performed through one-way ANOVA and Tukey multiple range analysis (SPSS 19.0 software (P <0.05). Results: Aspirin and Oxaliplatin considerably inhibited the growth of SW742 and SW480 cell lines. SW742 cell line was more sensitive to Aspirin than SW480 cell line. The cytotoxic effect of Oxaliplatin was higher than Aspirin in both cell lines. Conclusions: This study demonstrated that both Aspirin and Oxaliplatin have cytotoxic effects on SW742 and SW480 cell lines in vitro. Thus, Aspirin may be considered as a therapeutic agent in CRC, however, further in vivo investigations are required to fully establish this effect.

scholarly journals Antiproliferative activity and caspase enhancement properties of Annona muricata leaves extract against colorectal cancer cells

2016 ◽  
Vol 25 (3) ◽  
pp. 136-42
Author(s):  
Lili Indrawati ◽  
Purwantyastuti Ascobat ◽  
Budiman Bela ◽  
Murdani Abdullah ◽  
Ingrid S. Surono ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Water Extract ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Annona Muricata ◽  
Caspase 9

Background: The prevalence of colorectal cancer is rising in Asia including Indonesia. Annona muricata tea leaves, that is traditionally used for maintaining health, and lately being used by cancer patients. The objectives of this study is to investigate its effects in human colorectal cancer cell in vitro and ex vivo.Methods: Thirty patients with colorectal cancer (CRC) were enrolled in a randomized double-blind placebo-controlled trial. They were equally divided into two groups: those treated with 300 mg A. muricata leaf extract and placebo daily for 8 weeks. Serum from supplemented CRC patients of both groups was compared for caspase 9 and caspase 8 enhancement activity. Antiproliferative effect of water extract of A. muricata leaves and its fractions were evaluated against colorectal cancer cell line (DLD-1 and COLO 205) compared with 5-fluorouracil and placebo, the dose range was 62.5-2,000 µg/mL. Method used was 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Data were analyzed by Mann-Whitney U test. The p value was set at 0.05.Results: Ethanol-soluble fraction of A. muricata leaves extract water extract (ESFAM) leaves extract had cytotoxicity effects on DLD-1 as well as COLO 205 cell line, as shown by the lower IC50 compared to 5-fluorouracil and placebo, 20.59 μg/mL and 654.9μg/mL, respectively. Serum of subjects supplemented with extract significantly induced caspase 9 (p=0.001) activity of DLD-1 colorectal cancer cell line, but not for caspase 8 activity (p=0.372).Conclusion: The study's results suggest the cytotoxicity potential of  A. muricata  leaves extract  in in vitro and ex vivo studies.

scholarly journals Selection of a malignant subpopulation from a colorectal cancer cell line

2020 ◽  
Vol 20 (3) ◽  
pp. 2937-2945
Author(s):  
Pei‑Lun Lai ◽  
Ting‑Chun Chen ◽  
Chun‑Yen Feng ◽  
Hsuan Lin ◽  
Chi‑Hou Ng ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Selection Of

scholarly journals Development of a Potential Gallium-68-Labelled Radiotracer Based on DOTA-Curcumin for Colon-Rectal Carcinoma: From Synthesis to In Vivo Studies

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 644 ◽  
Author(s):  
Giulia Orteca ◽  
Federica Pisaneschi ◽  
Sara Rubagotti ◽  
Tracy Liu ◽  
Giacomo Biagiotti ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Human Lymphocytes ◽  
Cancer Cell Line ◽  
Cell Uptake ◽  
In Vivo Studies ◽  
Colorectal Cancer Cell Line ◽  
Preferential Uptake ◽  
Gallium 68

Colorectal cancer is the third most commonly occurring cancer in men and the second most commonly occurring cancer in women worldwide. We have recently reported that curcuminoid complexes labelled with gallium-68 have demonstrated preferential uptake in HT29 colorectal cancer and K562 lymphoma cell lines compared to normal human lymphocytes. In the present study, we report a new gallium-68-labelled curcumin derivative (68Ga-DOTA-C21) and its initial validation as marker for early detection of colorectal cancer. The precursor and non-radioactive complexes were synthesized and deeply characterized by analytical methods then the curcuminoid was radiolabelled with gallium-68. The in vitro stability, cell uptake, internalization and efflux properties of the probe were studied in HT29 cells, and the in vivo targeting ability and biodistribution were investigated in mice bearing HT29 subcutaneous tumour model. 68Ga-DOTA-C21 exhibits decent stability (57 ± 3% after 120 min of incubation) in physiological media and a curcumin-mediated cellular accumulation in colorectal cancer cell line (121 ± 4 KBq of radiotracer per mg of protein within 60 min of incubation). In HT29 tumour-bearing mice, the tumour uptake of 68Ga-DOTA-C21 is 3.57 ± 0.3% of the injected dose per gram of tissue after 90 min post injection with a tumour to muscle ratio of 2.2 ± 0.2. High amount of activity (12.73 ± 1.9% ID/g) is recorded in blood and significant uptake of the radiotracer occurs in the intestine (13.56 ± 3.3% ID/g), lungs (8.42 ± 0.8% ID/g), liver (5.81 ± 0.5% ID/g) and heart (4.70 ± 0.4% ID/g). Further studies are needed to understand the mechanism of accumulation and clearance; however, 68Ga-DOTA-C21 provides a productive base-structure to develop further radiotracers for imaging of colorectal cancer.

scholarly journals ERCC1 Induction after Oxaliplatin Exposure May Depend on KRAS Mutational Status in Colorectal Cancer Cell Line: In Vitro Veritas

2015 ◽  
Vol 6 (1) ◽  
pp. 70-81 ◽  
Author(s):  
A. Orlandi ◽  
M. Di Salvatore ◽  
C. Bagalà ◽  
M. Basso ◽  
A. Strippoli ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Mutational Status
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