scholarly journals Complete genome sequence and evolution analysis of Psychrobacter sp. YP14 from Gammaridea Gastrointestinal Microbiota of Yap Trench

2019 ◽  
Author(s):  
Wei Jiaqiang ◽  
Gao Zhiyuan ◽  
Liu Whenfeng ◽  
Chen Hao
Keyword(s):  
Stress Response ◽  
Complete Genome ◽  
Genome Structure ◽  
Gc Content ◽  
Rrna Genes ◽  
Trna Genes ◽  
Psychrophilic Bacterium ◽  
Putative Open Reading Frame ◽  
Gastrointestinal Microbiota ◽  
Circular Chromosome

AbstractPsychrobacter sp. YP14, a moderately psychrophilic bacterium belonging to the class Gammaproteobacteria, was isolated from Gammaridea Gastrointestinal Microbiota of Yap Trench. The strain has one circular chromosome of 2,895,311 bp with a 44.66% GC content, consisting of 2333 protein-coding genes, 53 tRNA genes and 9 rRNA genes. Four plasmids were completely assembled and their sizes were 13,712 bp, 19711 bp, 36270 bp, 8194 bp, respectively. In particular, a putative open reading frame (ORF) for dienelactone hydrolase (DLH) related to degradation of chlorinated aromatic hydrocarbons. To get an better understanding of the evolution of Psychrobacter sp. YP14 in this genus, six Psychrobacter strains (G, PRwf-1, DAB_AL43B, AntiMn-1,P11G5, P2G3), with publicly available complete genome, were selected and comparative genomics analysis were performed among them. The closest phylogenetic relationship was identified between strains G and K5 based on 16s gene and ANI (average nucleotide identity) values. Analysis of the pan-genome structure found that YP14 has fewer COG clusters associated with transposons and prophage which indicates fewer sequence rearrangements compared with PRwf-1. Besides, stress response-related genes of strain YP14 demonstrates that it has less strategies to cope with extreme environment, which is consistent with its intestinal habitat. The difference of metabolism and strategies coped with stress response of YP14 are more conducive to the study of microbial survival and metabolic mechanisms in deep sea environment.

scholarly journals Complete Genome Sequence of Megamonas funiformis Strain 1CBH44, Isolated from Human Feces

2021 ◽  
Vol 10 (48) ◽  
Author(s):  
Yusuke Ogata ◽  
Mitsuo Sakamoto ◽  
Naveen Kumar ◽  
Moriya Ohkuma ◽  
Masahira Hattori ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gc Content ◽  
Rrna Genes ◽  
Trna Genes ◽  
Human Feces ◽  
Circular Chromosome ◽  
Protein Coding ◽  
Content Type

Here, we report the complete genome sequence of Megamonas funiformis strain 1CBH44, which was isolated from the feces of a healthy Japanese person. The genome consists of a circular chromosome (2,310,709 bp, with a GC content of 31.5%) and possesses 2,170 putative protein-coding genes, 18 rRNA genes, and 54 tRNA genes.

scholarly journals Complete Genome Sequence of Thiohalobacter sp. Strain COW1, Isolated from Activated Sludge Treating Coke Oven Wastewater

2021 ◽  
Vol 10 (7) ◽  
Author(s):  
Kentaro Miyazaki ◽  
Hikaru Suenaga ◽  
Mamoru Oshiki ◽  
Shuichi Kawano ◽  
Toshikazu Fukushima
Keyword(s):  
Activated Sludge ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Coke Oven ◽  
Rrna Genes ◽  
Trna Genes ◽  
Circular Chromosome ◽  
Protein Coding ◽  
Degrading Bacterium

ABSTRACT A thiocyanate-degrading bacterium, Thiohalobacter sp. strain COW1, was isolated from activated sludge treating coke oven wastewater, and the complete genome sequence was determined. COW1 contained a single circular chromosome (3.23 Mb; G+C content, 63.4%) in which 2,788 protein-coding genes, 39 tRNA genes, and 3 rRNA genes were identified.

scholarly journals Complete genome sequence of Chryseobacterium sp. ZHDP1 with protease activity

2021 ◽  
Author(s):  
Huanshuai Zhao ◽  
Jianxin Wang ◽  
Jiamao Huang ◽  
Yuncheng Ma ◽  
Yunfei Chen ◽  
...  
Keyword(s):  
Complete Genome ◽  
Protease Activity ◽  
Fermentation Broth ◽  
Gc Content ◽  
Rrna Genes ◽  
Trna Genes ◽  
Rrna Gene ◽  
The Novel ◽  
Maximum Similarity ◽  
Insight Into

Abstract This study reported a complete genome of Chryseobacterium sp. ZHDP1 isolated from the soils of a seafood market. The ZHDP1 genome with a size of 4,917,748 bp and a GC content of 35.95% possessed 4,478 coding genes, 5 rRNA genes, 26 sRNA genes, and 89 tRNA genes. The 16S rRNA gene sequence of ZHDP1 had a maximum similarity of 99.07% with that of C. gambrini 5-1St1a. The maximum values of average nucleotide identity and DNA-DNA hybridization of ZHDP1 genome were 91.39 and 47.8, respectively, which were lower than the thresholds for a new genome. Different protease genes were annotated in the genome of ZHDP1, and the protease activity was also detected in the fermentation broth of ZHDP1. Furthermore, the activity of protease in the fermentation broth was optimized through temperature, pH, and metal irons, and the results showed that 60°C and pH 7.0 were the optimum conditions and Fe3+ could positively increase the protease activity of ZHDP1. This study provides the first insight into the novel genomic information of Chryseobacterium sp. ZHDP1 and its protein-degrading ability, thereby broadening our knowledge of the industrial potentials in genus Chryseobacterium strains.

scholarly journals Complete genome sequence analysis of a novel granaticin producer, Streptomyces sp. A1013Y

2020 ◽  
Author(s):  
Shenglan Zheng ◽  
Yunping Zhu ◽  
Kaifeng Liu ◽  
Jinlong Li
Keyword(s):  
Complete Genome ◽  
Deletion Mutant ◽  
Gc Content ◽  
Rrna Genes ◽  
Trna Genes ◽  
Genome Sequence Analysis ◽  
Streptomyces Sp ◽  
Fermentation Liquid ◽  
Complete Genome Sequence Analysis ◽  
Genome Information

Abstract Granaticin is a kind of antibiotics with the function of anticancer, antibacterial, anticoccidial. Streptomyces sp. A1013Y is a new strain isolated from soil with granaticin-producing ability. The genome information was analyzed for the further study of granaticin. The present work reported the complete genome of S. sp. A1013Y, which contains a 7,646,296 bp chromosome with an average GC content of 71.59%. A total of 7361 CDSs, including 66 tRNA genes, 18 rRNA genes and 23 clusters, were identified in the genome. With homologous recombination, an in-frame deletion mutant was constructed to confirm the granaticin cluster. The fermentation liquid of the mutant did not contain granaticin based on HPLC and LC-MS analysis, indicating that S. sp. A1013Y is a granaticin producer. Comparing the granaticin biosynthesis cluster of S. sp. A1013Y with Streptomycesviolaceoruber Tü22 and Streptomyces vietnamensis GIMV4.0001T, which were sequenced and produce granaticin, the similarity between the granaticin clusters was 78% and 83% respectively, but some genes still weren’t identified in the granaticin biosynthesis cluster. The phylogenetic analysis and ANI value between S. sp. A1013Y and other Streptomyces species, including S. vietnamensis GIMV4.0001T, were all below 85%, which showed that S. sp. A1013Y was probably a novel streptomyces strain that produced granaticin. The whole genome information of S. sp. A1013Y provides a valuable foundation for future granaticin analyses as well as biomedicine applications.

scholarly journals Complete Genome Sequence of Blautia producta JCM 1471T

2020 ◽  
Vol 9 (17) ◽  
Author(s):  
Dieter M. Tourlousse ◽  
Mitsuo Sakamoto ◽  
Takamasa Miura ◽  
Koji Narita ◽  
Akiko Ohashi ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Rrna Genes ◽  
Trna Genes ◽  
Circular Chromosome ◽  
Protein Coding ◽  
Coding Sequences ◽  
Content Type ◽  
Single Circular Chromosome

We report a complete genome sequence of Blautia producta JCM 1471T. The genome consists of a single circular chromosome of 6,197,116 bp with a G+C content of 45.7%. The genome was annotated as containing 5 complete sets of rRNA genes, 70 tRNA genes, and 5,516 protein-coding sequences.

scholarly journals Complete Genome Sequence of Lactobacillus helveticus JCM 1004, an Aminopeptidase-Producing Lactic Acid Bacterium

2021 ◽  
Vol 10 (33) ◽  
Author(s):  
Kana Morinaga ◽  
Hiroyuki Kusada ◽  
Miho Watanabe ◽  
Hideyuki Tamaki
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Lactobacillus Helveticus ◽  
Rrna Genes ◽  
Trna Genes ◽  
Circular Chromosome ◽  
Content Type

We report the complete genome sequence of Lactobacillus helveticus JCM 1004, an aminopeptidase-producing lactic acid bacterium. The genome consists of a circular chromosome which comprises 2,261,280 bp, with a G+C content of 37.56%. The genome was predicted to harbor 13 rRNA genes, 64 tRNA genes, and 2,462 protein-coding sequences.

scholarly journals Complete genome sequence analysis of a novel granaticin producer, Streptomyces sp. A1013Y

2019 ◽  
Author(s):  
Shenglan Zheng ◽  
Yunping Zhu ◽  
Kaifeng Liu ◽  
Jinlong Li
Keyword(s):  
Complete Genome ◽  
Deletion Mutant ◽  
Gc Content ◽  
Rrna Genes ◽  
Trna Genes ◽  
Streptomyces Species ◽  
Streptomyces Sp ◽  
Fermentation Liquid ◽  
Complete Genome Sequence Analysis ◽  
Genome Information

Abstract Granaticin is a kind of antibiotics with the function of anticancer, antibacterial, anticoccidial. Streptomyces sp. A1013Y is a new strain isolated from soil with granaticin-producing ability. The genome information was analyzed for the further study of granaticin. The present work reported the complete genome of S. sp. A1013Y, which contains a 7,646,296 bp chromosome with an average GC content of 71.59%. A total of 7361 CDSs, including 66 tRNA genes, 18 rRNA genes and 23 clusters, were identified in the genome. With homologous recombination, an in-frame deletion mutant was constructed to confirm the granaticin cluster. The fermentation liquid of the mutant did not contain granaticin based on HPLC and LC-MS analysis, indicating that S. sp. A1013Y is a granaticin producer. Comparing the granaticin biosynthesis cluster of S. sp. A1013Y with Streptomycesviolaceoruber Tü22 and Streptomyces vietnamensis GIMV4.0001T, which were sequenced and produce granaticin, the similarity between the granaticin clusters was 78% and 83% respectively, but some genes still weren’t identified in the granaticin biosynthesis cluster. The phylogenetic analysis and ANI value between S. sp. A1013Y and other Streptomyces species, including S. vietnamensis GIMV4.0001T, were all below 85%, which showed that S. sp. A1013Y was probably a novel streptomyces strain that produced granaticin. The whole genome information of S. sp. A1013Y provides a valuable foundation for future granaticin analyses as well as biomedicine applications.

scholarly journals Complete Genome Sequence of Flavonifractor plautii JCM 32125T

2020 ◽  
Vol 9 (17) ◽  
Author(s):  
Dieter M. Tourlousse ◽  
Mitsuo Sakamoto ◽  
Takamasa Miura ◽  
Koji Narita ◽  
Akiko Ohashi ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Rrna Genes ◽  
Trna Genes ◽  
Circular Chromosome ◽  
Protein Coding ◽  
Coding Sequences ◽  
Content Type ◽  
Single Circular Chromosome

We report the complete genome sequence of Flavonifractor plautii JCM 32125T (=VPI 0310T). The genome consists of a single circular chromosome of 3,985,392 bp (G+C content, 60.9%) and was predicted to contain 3 complete sets of rRNA genes, 63 tRNA genes, and 3,764 protein-coding sequences.

scholarly journals A genomic perspective on the potential of termite-associated Cellulosimicrobium cellulans MP1 as producer of plant biomass-acting enzymes and exopolysaccharides

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11839
Author(s):  
Nguyen Thi-Hanh Vu ◽  
Tung Ngoc Quach ◽  
Xuan Thi-Thanh Dao ◽  
Ha Thanh Le ◽  
Chi Phuong Le ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Draft Genome ◽  
Gc Content ◽  
Rrna Genes ◽  
Trna Genes ◽  
Rrna Gene ◽  
Whole Genome ◽  
Potential Candidate ◽  
Circular Chromosome ◽  
Cellulosimicrobium Cellulans

Background Lignocellulose is a renewable and enormous biomass resource, which can be degraded efficiently by a range of cocktails of carbohydrate-active enzymes secreted by termite gut symbiotic bacteria. There is an urgent need to find enzymes with novel characteristics for improving the conversion processes in the production of lignocellulosic-based products. Although various studies dedicated to the genus Cellulosimicrobium as gut symbiont, genetic potential related to plant biomass-acting enzymes and exopolysaccharides production has been fully untapped to date. Methods The cellulolytic bacterial strain MP1 was isolated from termite guts and identified to the species level by phenotypic, phylogenetic, and genomic analysis. To further explore genes related to cellulose and hemicellulose degradation, the draft genome of strain MP1 was obtained by using whole-genome sequencing, assembly, and annotation through the Illumina platform. Lignocellulose degrading enzymes and levan production in the liquid medium were also examined to shed light on bacterial activities. Results Among 65 isolates obtained, the strain MP1 was the most efficient cellulase producer with cellulase activity of 0.65 ± 0.02 IU/ml. The whole genome analysis depicted that strain MP1 consists of a circular chromosome that contained 4,580,223 bp with an average GC content of 73.9%. The genome comprises 23 contigs including 67 rRNA genes, three tRNA genes, a single tmRNA gene, and 4,046 protein-coding sequences. In support of the phenotypic identification, the 16S rRNA gene sequence, average nucleotide identity, and whole-genome-based taxonomic analysis demonstrated that the strain MP1 belongs to the species Cellulosimicrobium cellulans. A total of 30 genes related to the degradation of cellulases and hemicellulases were identified in the C. cellulans MP1 genome. Of note, the presence of sacC1-levB-sacC2-ls operon responsible for levan and levan-type fructooligosaccharides biosynthesis was detected in strain MP1 genome, but not with closely related C. cellulans strains, proving this strain to be a potential candidate for further studies. Endoglucanases, exoglucanases, and xylanase were achieved by using cheaply available agro-residues such as rice bran and sugar cane bagasse. The maximum levan production by C. cellulans MP1 was 14.8 ± 1.2 g/l after 20 h of cultivation in media containing 200 g/l sucrose. To the best of our knowledge, the present study is the first genome-based analysis of a Cellulosimicrobium species which focuses on lignocellulosic enzymes and levan biosynthesis, illustrating that the C. cellulans MP1 has a great potential to be an efficient platform for basic research and industrial exploitation.

scholarly journals Complete Genome Sequence of Collinsella aerofaciens JCM 10188T

2020 ◽  
Vol 9 (16) ◽  
Author(s):  
Dieter M. Tourlousse ◽  
Mitsuo Sakamoto ◽  
Takamasa Miura ◽  
Koji Narita ◽  
Akiko Ohashi ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Rrna Genes ◽  
Trna Genes ◽  
Circular Chromosome ◽  
Content Type ◽  
Extrachromosomal Elements ◽  
Protein Encoding

We report a complete genome sequence of Collinsella aerofaciens JCM 10188T (=VPI 1003T). The genome consists of a circular chromosome (2,428,218 bp with 60.6% G+C content) and two extrachromosomal elements. The genome was predicted to contain 5 sets of rRNA genes, 58 tRNA genes, and 2,079 protein-encoding sequences.

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