scholarly journals Drought Affects the Antioxidant System and Stomatal Aperture in Zanthoxylum bungeanum Maxim

2019 ◽  
Author(s):  
Xitong Fei ◽  
Haichao Hu ◽  
Jingmiao Li ◽  
Yulin Liu ◽  
Anzhi Wei
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Antioxidant System ◽  
Expression Patterns ◽  
Stomatal Aperture ◽  
Drought Duration ◽  
Altered Gene Expression ◽  
Altered Gene ◽  
Zanthoxylum Bungeanum ◽  
The Relationship

AbstractWhen under drought, plants activate a range of self-protective responses. Among these are activation of the antioxidant system and changes in stomatal aperture. The antioxidant system can remove the reactive oxygen species produced under drought conditions and so mitigate oxidative damage. Water becomes a severely limiting resource for plants suffering drought stress, so they generally close their stomata to reduce water loss. We examined changes in the activities of the antioxidant enzymes and altered gene expression patterns in Zanthoxylum bungeanum plants exposed to drought by irrigation with 20% PEG6000. We also recorded changes in stomatal aperture as the drought persisted. Relationships between the antioxidant system and stomatal aperture were analyzed in relation to gene expression. The results indicate that under drought stress, POD, CAT, APX, proline, MDA and related genes all show positive responses to drought, while SOD and its genes showed a negative response. The relationship between drought duration and stomatal aperture was considered. Stomatal aperture declines exponentially as drought duration increases.

Altered gene expression patterns in ART offspring

2004 ◽  
Vol 82 ◽  
pp. S292 ◽  
Author(s):  
H.J. Kang ◽  
Y. Katagiri ◽  
Q.V. Neri ◽  
R. Baergen ◽  
Z. Rosenwaks ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Altered Gene Expression ◽  
Altered Gene

scholarly journals Altered gene expression patterns in muscle ring finger 1 null mice during denervation- and dexamethasone-induced muscle atrophy

2013 ◽  
Vol 45 (23) ◽  
pp. 1168-1185 ◽  
Author(s):  
J. David Furlow ◽  
Monica L. Watson ◽  
David S. Waddell ◽  
Eric S. Neff ◽  
Leslie M. Baehr ◽  
...  
Keyword(s):  
Gene Expression ◽  
Muscle Atrophy ◽  
Intracellular Signaling ◽  
Expression Patterns ◽  
Ring Finger ◽  
Altered Gene Expression ◽  
Catabolic State ◽  
Altered Gene ◽  
Multiple Conditions

Muscle atrophy can result from inactivity or unloading on one hand or the induction of a catabolic state on the other. Muscle-specific ring finger 1 (MuRF1), a member of the tripartite motif family of E3 ubiquitin ligases, is an essential mediator of multiple conditions inducing muscle atrophy. While most studies have focused on the role of MuRF1 in protein degradation, the protein may have other roles in regulating skeletal muscle mass and metabolism. We therefore systematically evaluated the effect of MuRF1 on gene expression during denervation and dexamethasone-induced atrophy. We find that the lack of MuRF1 leads to few differences in control animals, but there were several significant differences in specific sets of genes upon denervation- and dexamethasone-induced atrophy. For example, during denervation, MuRF1 knockout mice showed delayed repression of metabolic and structural genes and blunted induction of genes associated with the neuromuscular junction. In the latter case, this pattern correlates with blunted HDAC4 and myogenin upregulation. Lack of MuRF1 caused fewer changes in the dexamethasone-induced atrophy program, but certain genes involved in fat metabolism and intracellular signaling were affected. Our results demonstrate a new role for MuRF1 in influencing gene expression in two important models of muscle atrophy.

Altered gene expression patterns in intrauterine growth restriction: Potential role of hypoxia

2007 ◽  
Vol 196 (1) ◽  
pp. 70.e1-70.e6 ◽  
Author(s):  
Cathal McCarthy ◽  
Finbarr E. Cotter ◽  
Suzanne McElwaine ◽  
Anne Twomey ◽  
Eoghan E. Mooney ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intrauterine Growth Restriction ◽  
Potential Role ◽  
Expression Patterns ◽  
Growth Restriction ◽  
Gene Expression Patterns ◽  
Intrauterine Growth ◽  
Altered Gene Expression ◽  
Altered Gene

scholarly journals Identification of microRNA-target interactions for antioxidant defense response under drought stress by high-throughput sequencing in Zanthoxylum bungeanum Maxim

2019 ◽  
Author(s):  
Xitong Fei ◽  
Haichao Hu ◽  
Jingmiao Li ◽  
Yulin Liu ◽  
Anzhi Wei
Keyword(s):  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Antioxidant System ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Expression Patterns ◽  
Interaction Model ◽  
Normal Growth ◽  
Microrna Target ◽  
Zanthoxylum Bungeanum

ABSTRACTWhen the plant is in an unfavorable environment such as drought or high temperature, it will accumulate a large amount of active oxygen, which will seriously affect the normal growth and development of the plant. The antioxidant system can remove the reactive oxygen species produced under drought conditions and so mitigate oxidative damage. We examined the trends of antioxidant enzymes, miRNAs and their target genes in Zanthoxylum bungeanum under drought stress. According to the changes of antioxidant enzymes, miRNAs and their target genes expression patterns of Zanthoxylum bungeanum under drought stress, an interaction model was constructed to provide a reference for further understanding of plant antioxidant mechanism. The results indicate that under drought stress, POD, CAT, APX, proline, MDA and related genes all show positive responses to drought, while SOD and its genes showed a negative response. It is speculated that in the antioxidant process of Zanthoxylum bungeanum, POD, CAT, and APX play a major role, and SOD plays a supporting role. In addition, the expression levels of miRANs and their target genes were basically negatively correlated, indicating that miRNAs are involved in the regulation of the antioxidant system of Zanthoxylum bungeanum.

199 IN VITRO-DEVELOPED BOVINE BLASTOCYSTS ARE MARKED WITH ABERRANT HYPER- AND HYPO-METHYLATED GENOMIC REGIONS

2015 ◽  
Vol 27 (1) ◽  
pp. 190
Author(s):  
D. Salilew-Wondim ◽  
M. Hoelker ◽  
U. Besenfelder ◽  
V. Havlicek ◽  
F. Rings ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Expression Patterns ◽  
Proximal Promoter ◽  
Altered Gene Expression ◽  
Genome Wide ◽  
Altered Gene ◽  
Genomic Regions

Most often, in vitro produced embryos display poor quality and altered gene expression patterns compared to their in vivo counterparts. Aberrant DNA methylation occurring during in vitro embryo development is believed to be one of the multifaceted factors which may cause altered gene expression and poor embryo quality. Here, we investigated the genome-wide DNA methylation patterns of in vitro derived embryos using the recently developed Bovine EmbryoGENE Methylation Platform (BEGMP) array (Shojaei Saadi et al. BMC Genomics 2014 15, 451. doi: 10.1186/1471-2164-15-451) to unravel the aberrantly methylated genomic region in in vitro developed embryos. For this, in vitro and in vivo produced blastocysts were produced and used for genome-wide DNA methylation analysis. In vitro blastocysts were produced from oocytes retrieved from ovaries collected from the local abattoir and matured, fertilized, and cultured in vitro using SOF media. The in vivo blastocysts were produced by superovulation and AI of Simmental heifers followed by uterine flushing. Genomic DNA (gDNA) was then isolated from four replicates (each 10 blastocysts) of in vivo and in vitro derived blastocysts using Allprep DNA/RNA micro kit (Qiagen, Valencia, CA, USA) and the gDNA was then fragmented using the MseI enzyme. Following this, MseLig21 and MseLig were ligated to the MseI-digested genomic fragments in the presence of Ligase enzyme. Methyl-sensitive enzymes, HpaII, AciI, and Hinp1I, were used to cleave unmethlayted genomic regions within the MseI-MseI region of the fragmented DNA. The gDNA was subjected to two rounds of ligation-mediated polymerase chain reaction (LM-PCR) amplification. After removal of the adapters, the amplified gDNA samples from in vivo or in vitro groups were labelled either Cy-3 or Cy-5 dyes in dye-swap design using ULS Fluorescent gDNA labelling kit (Kreatech Biotechnology BV, Amsterdam, The Netherlands). Hybridization was performed for 40 h at 65°C. Slides were scanned using Agilent's High-Resolution C Scanner (Agilent Technologies Inc., Santa Clara, CA, USA) and features were extracted with Agilent's Feature Extraction software (Agilent Technologies Inc.). The results have shown that from a total of 414 566 probes harboured by the BEGMP array, 248 453 and 253 147 probes were detected in in vitro and in vivo derived blastocysts, respectively. Data analysis using the linear modelling for microarray (LIMMA) package and R software (The R Project for Statistical Computing, Vienna, Austria) revealed a total of 3434 differentially methylated regions (DMRs; Fold change ≥1.5, P-value <0.05), of which 42 and 58% were hyper- and hypo-methylated, respectively, in in vitro derived blastocysts compared to their in vivo counterparts. The DMRs were found to be localised in the intronic, exonic, promoter, proximal promoter, and distal promoter, and some of the probes did not have nearby genes. In addition, 10.8% of the DMRs were found to be stretched in short, long, or intermediate CpG islands. Thus, this study demonstrated genome-wide dysregulation in the epigenome landscape of in vitro-derived embryos by the time they reach to the blastocysts stage.

Role of potential COVID-19 immune system associated genes and the potential pathways linkage with type-2 diabetes

2021 ◽  
Vol 24 ◽  
Author(s):  
Nawal Helmi ◽  
Dalia Alammari ◽  
Mohammad Mobashir
Keyword(s):  
Gene Expression ◽  
Type 2 Diabetes ◽  
Immune System ◽  
Expression Patterns ◽  
Osteoclast Differentiation ◽  
Gene Expression Pattern ◽  
Common Source ◽  
Altered Gene Expression ◽  
Altered Gene

Background: Coronavirus is an enveloped positive-sense RNA virus and is characterized by club-like spikes projecting from its surface which is commonly associated with acute respiratory infections in humans but its ability to infect multiple host species and multiple diseases brings it to a complex pathogen group. The frequent interactions of wild animals with humans it is more prevalent a common source of such infections and SARS—CoV and MERS—CoV are the zoonotic pathogens among the leading cause of severe respiratory diseases in humans. Aim: The major purpose of this study was to study the gene expression profiling for those human samples which are infected with coronavirus or uninfected and compare the differential expression patterns and its functional impact. Methods: For this purpose, the previously studied samples have been collected from public database and the study had been performed and it includes gene expression analysis, pathway analysis, and the network-level understanding. The analysis presents the data for the differentially expressed genes, enriched pathways and the networks for the potential genes and gene sets. In terms of gene expression and the linkage of COVID-19 with type-2 diabetes. Results: We observe that there are a large number of genes which show altered gene expression pattern than the normal for coronavirus infection while in terms of pathways it appears that there are few sets of functions which are affected due to altered gene expression and they infer to infection, inflammation, and the immune system. Conclusions: Based on our study, we conclude that the potential genes which are affected due to infection are NFKBIA, MYC, FOXO3, BIRC3, ICAM1, IL8, CXCL1/2/5, GADD45A, RELB, SGK1, AREG, BBC3, DDIT3/4, EGR1, MTHFD2, and SESN2 and the functional changes are mainly associated with these pathways TNF, cytokine, NF—kB, TLR, TCR, BCR, Foxo, and TGF signaling pathways are among them and there are additional pathways such as hippo signaling, apoptosis, estrogen signaling, regulating pluropotency of stem cells, ErbB, Wnt, p53, cAMP, MAPK, PI3K—AKT, oxidative phosphorylation, protein processing in endoplasmic reticulum, prolactin signaling, adipocytokine, neurotrophine signaling, and longevity regulating pathways. SMARCD3, PARL, GLIPR1, STAT2, PMAIP1, GP1BA, and TOX genes and PI3K-Akt, focal adhesion, Foxo, phagosome, adrenergic, osteoclast differentiation, platelet activation, insulin, cytokine-cytokine interaction, apoptosis, ECM, JAK-STAT, and oxytocine signaling appear as the linkage between COVID-19 and Type-2 diabetes.

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