scholarly journals MicroRNA-transcriptome networks in whole blood and monocytes of women undergoing preterm labor

2019 ◽  
Author(s):  
Alison G. Paquette ◽  
Oksana Shynlova ◽  
Xiaogang Wu ◽  
Mark Kibschull ◽  
Kai Wang ◽  
...  
Keyword(s):  
Gestational Age ◽  
Mirna Expression ◽  
Whole Blood ◽  
Preterm Labor ◽  
Interleukin 2 ◽  
Neonatal Morbidity ◽  
Small Rna Sequencing ◽  
Immune Pathways ◽  
Mirna Concentration ◽  
Shed Light

ABSTRACTPreterm birth is attributed to neonatal morbidity as well as cognitive and physiological challenges. We have previously identified significant differences in mRNA expression in whole blood and monocytes, as well as differences in miRNA concentration in blood plasma, extracellular vesicles (EV) and EV-depleted plasma in women undergoing spontaneous preterm labor (sPTL). The goal of this analysis was to identify differences in miRNA expression within whole blood (WB) and peripheral monocytes (PM) from the same population of women undergoing sPTL compared to nonlaboring controls matched by gestational age. We performed single end small RNA sequencing in whole blood and peripheral monocytes from women undergoing sPTL with active contractions(24-34 weeks of gestation, N=15) matched for gestational age to healthy pregnant non laboring controls (>37 weeks gestation, N=30) who later delivered at term as a part of the Ontario Birth Study (Toronto, Ontario CA). We identified significant differences in expression of 16 miRNAs in PMs and 9 miRNAs in WB in women undergoing sPTL. In PMs, these miRNAs were predicted targets of 541 genes, including 28 previously associated with sPTL. In WB, miRNAs were predicted to target 303 genes, including 9 previously associated with sPTL. These genes were involved in a variety of immune pathways, including interleukin 2 signaling. This study is the first to identify changes in miRNA expression in WB and PMs of women undergoing sPTL. Our results shed light on potential mechanisms by which miRNAs may play a role in mediating systemic inflammatory response in pregnant women that deliver prematurely.

scholarly journals KARAKTERISTIK PERSALINAN PREMATUR DI RSUP PROF. DR. R. D. KANDOU MANADO

e-CliniC ◽  
2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Siva Oroh ◽  
Eddy Suparman ◽  
Hermie M. M. Tendean
Keyword(s):  
High School ◽  
General Hospital ◽  
Gestational Age ◽  
Premature Birth ◽  
Preterm Labor ◽  
Neonatal Morbidity ◽  
Descriptive Study ◽  
High School Education ◽  
Age Group ◽  
History Of

Abstract: Preterm labor is labor that occurs at 20 - <37 weeks gestational age calculated from the first day of the last menstrual period. Preterm labor is one cause of neonatal morbidity and mortality, which is 60-80% worldwide. This was a retrospective and descriptive study using the medical record of the Department of Obstetrics & Gynecology/Prof. Dr. R.D. Kandou General Hospital Manado period of January 1st to December 31st 2013. There were 151 premature labors during that period. The most frequent characteristics were age group 21-34 years, high school education, and house wife. Based on the parity status, the highest number was P0. History of abortion was found only in a small part of samples. The highest number of diseases/complications was rupture of membrane. The most frequent type of labor was spontaneous labor with back of the head position.Keywords: premature birth, premature babyAbstrak: Persalinan prematur adalah persalinan yang berlangsung pada usia kehamilan 20 - <37 minggu dihitung dari hari pertama haid terakhir. Persalinan prematur merupakan salah satu penyebab utama morbiditas dan mortalitas neonatal, yaitu 60-80% di seluruh dunia. Penelitian ini bersifat deskriptif retrospektif dengan memanfaatkan data sekunder berupa catatan medik di Bagian Obstetri Ginekologi/BLU RSUP Prof. Dr. R. D. Kandou Manado periode 1 Januari-31 Desember 2013. Hasil penelitian mendapatkan 151 sampel kasus persalinan prematur. Karakteristik berdasarkan usia ibu terbanyak pada usia 21-34 tahun, pendidikan ibu terbanyak SMA, dan pekerjaan IRT. Berdasarkan paritas didapatkan jumlah tertinggi pada P0, riwayat abortus hanya sebagian kecil ibu, penyakit/penyulit tersering ialah ketuban pecah dini, dan jenis persalinan tersering ialah spontan letak belakang kepala.Kata kunci: persalinan prematur, bayi prematur

Neonatal Outcomes at Extreme Prematurity by Gestational Age Versus Birth Weight in a Contemporary Cohort

2021 ◽  
Author(s):  
Elizabeth B. Ausbeck ◽  
Phillip Hunter Allman ◽  
Jeff M. Szychowski ◽  
Akila Subramaniam ◽  
Anup Katheria
Keyword(s):  
Birth Weight ◽  
Umbilical Cord ◽  
Gestational Age ◽  
Predictive Value ◽  
Neonatal Death ◽  
Controlled Trial ◽  
Neonatal Morbidity ◽  
Mortality And Morbidity ◽  
Severe Morbidity ◽  
Multicenter Randomized Controlled Trial

Objective The aim of the study is to describe the rates of neonatal death and severe neonatal morbidity in a contemporary cohort, as well as to evaluate the predictive value of birth gestational age (GA) and birth weight, independently and combined, for neonatal mortality and morbidity in the same contemporary cohort. Study Design We performed a secondary analysis of an international, multicenter randomized controlled trial of delayed umbilical cord clamping versus umbilical cord milking in preterm infants born at 23 0/7 to 31 6/7 weeks of gestation. The current analysis was restricted to infants delivered <28 weeks. The primary outcomes of this analysis were neonatal death and a composite of severe neonatal morbidity. Incidence of outcomes was compared by weeks of GA, with planned subanalysis comparing small for gestational age (SGA) versus non-SGA neonates. Multivariable logistic regression was then used to model these outcomes based on birth GA, birth weight, or a combination of both as primary independent predictors to determine which had superior ability to predict outcomes. Results Of 474 neonates in the original trial, 180 (38%) were included in this analysis. Overall, death occurred in 27 (15%) and severe morbidity in 139 (77%) neonates. Rates of mortality and morbidity declined with increasing GA (mortality 54% at 23 vs. 9% at 27 weeks). SGA infants (n = 25) had significantly higher mortality compared with non-SGA infants across all GAs (p < 0.01). There was no difference in the predictive value for neonatal death or severe morbidity between the three prediction options (GA, birth weight, or GA and birth weight). Conclusion Death and severe neonatal morbidity declined with advancing GA, with higher rates of death in SGA infants. Birth GA and birth weight were both good predictors of outcomes; however, combining the two was not more predictive, even in SGA infants. Key Points

Premature birth (Clinical lecture)

2018 ◽  
pp. 9-12
Author(s):  
I.B. Ventskovskaya ◽  
◽  
V.V. Bila ◽  
O.S. Countryside ◽  
◽  
...  
Keyword(s):  
Magnesium Sulfate ◽  
Key Words ◽  
Gestational Age ◽  
Evidence Based Medicine ◽  
Premature Birth ◽  
Preterm Labor ◽  
Evidence Based ◽  
Advantages And Disadvantages ◽  
Based Medicine ◽  
Tocolytic Agents

The article presents modern views on the pathogenesis of preterm labor, their relevance and classification. From the perspective of evidence-based medicine methods of prevention are considered. A comparison of the main tocolytic agents, their advantages and disadvantages is presented. Key words: premature birth, perinatal and infantile mortality, tocolysis, magnesium sulfate, gestational age.

Three-dimensional ultrasound first trimester fetal volume measurement and its relation to pregnancy outcome

2017 ◽  
Vol 45 (9) ◽  
Author(s):  
Haitham A. Torky ◽  
Asem A. Moussa ◽  
Ali M. Ahmad ◽  
Osama Dief ◽  
Manar A. Eldesoouky ◽  
...  
Keyword(s):  
Birth Weight ◽  
Low Birth Weight ◽  
Pregnancy Outcome ◽  
Gestational Age ◽  
Preterm Labor ◽  
Three Dimensional ◽  
First Trimester ◽  
3D Ultrasound ◽  
Secondary Outcome ◽  
Crown Rump Length

AbstractAim of work:To determine whether fetal volume (FV) measured by three-dimensional (3D) ultrasound was able to detect fetuses at risk of low birth weight (primary outcome) and/or preterm labor (secondary outcome).Methods:One hundred pregnant women carrying a singleton living pregnancy who were sure of dates, and had a dating scan, with gestational age between 11 weeks and 13 weeks+6 days coming for routine first trimester nuchal translucency (NT) were examined by both two-dimensional (2D) and 3D ultrasound (Vocal System) for crown-rump length (CRL) and FV then followed up regularly every 4 weeks until 28 weeks then biweekly until 36 weeks then weekly until delivery both clinically and by ultrasound biometry.Findings:Eighty-seven cases had a normal outcome, while the remaining 13 cases had either preterm labor (four cases) or low-birth weight (nine cases). FV positively correlated with CRL (P=0.026), gestational age in weeks (P=0.002), neonatal body weight in grams (P=0.018) and neonatal body length at birth (P=0.04). A mean FV of 8.3 mmConclusion:3D assessment of FV in the first trimester provides an accurate method for predicting pregnancy outcome namely low birth weight and neonatal complications, however, it is a better positive predictor than a negative one.

scholarly journals Pregnancy Outcome of Twin Pregnancy at Gandaki Medical College Teaching Hospital, Pokhara, Nepal

2018 ◽  
Vol 11 (02) ◽  
pp. 1-4
Author(s):  
M Tripathi ◽  
R Shrestha
Keyword(s):  
Preterm Delivery ◽  
Teaching Hospital ◽  
Gestational Age ◽  
Twin Pregnancy ◽  
College Teaching ◽  
Neonatal Morbidity ◽  
Maternal Complications ◽  
Cross Sectional ◽  
Neonatal Complications ◽  
Twin Pregnancies

Objectives: To evaluate maternal and neonatal complications and pregnancy outcomes of twin pregnancies. Methods: The cross sectional study was conducted using retrospective data on the twin pregnancies with more than 28 weeks of gestational age. The study used data over a period of five years, from March 10, 2010 to March 9, 2015 in the Department of Obstetrics and Gynecology, GMC Teaching Hospital Pokhara. Results: Of the 50 twin pregnancies, the most common maternal complication was preterm delivery (40%). Other maternal complications were anemia (36%), pregnancy induced hypertension (14%), premature rupture of membranes (14%), postpartum hemorrhage (12%) and antepartum hemorrhage (6%). Median gestational age at delivery was 37 weeks. Most common route of delivery was cesarean section (66%). Most common neonatal complication was low birth weight (48%) births first twin and second twin 56%. Conclusion: Twin pregnancy has high maternal and neonatal complications, especially preterm delivery that increases the risk of significant neonatal morbidity and mortality.

scholarly journals xMD-miRNA-seq to generate near in vivo miRNA expression estimates in colon epithelial cells

2018 ◽  
Author(s):  
Avi Z. Rosenberg ◽  
Carrie Wright ◽  
Karen Fox-Talbot ◽  
Anandita Rajpurohit ◽  
Courtney Williams ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Small Rna ◽  
Mirna Expression ◽  
Low Cost ◽  
Expression Patterns ◽  
Small Rna Sequencing ◽  
Rna Seq ◽  
Cell Type

AbstractAccurate, RNA-seq based, microRNA (miRNA) expression estimates from primary cells have recently been described. However, this in vitro data is mainly obtained from cell culture, which is known to alter cell maturity/differentiation status, significantly changing miRNA levels. What is needed is a robust method to obtain in vivo miRNA expression values directly from cells. We introduce expression microdissection miRNA small RNA sequencing (xMD-miRNA-seq), a method to isolate cells directly from formalin fixed paraffin-embedded (FFPE) tissues. xMD-miRNA-seq is a low-cost, high-throughput, immunohistochemistry-based method to capture any cell type of interest. As a proof-of-concept, we isolated colon epithelial cells from two specimens and performed low-input small RNA-seq. We generated up to 600,000 miRNA reads from the samples. Isolated epithelial cells, had abundant epithelial-enriched miRNA expression (miR-192; miR-194; miR-200b; miR-200c; miR-215; miR-375) and overall similar miRNA expression patterns to other epithelial cell populations (colonic enteroids and flow-isolated colon epithelium). xMD-derived epithelial cells were generally not contaminated by other adjacent cells of the colon as noted by t-SNE analysis. xMD-miRNA-seq allows for simple, economical, and efficient identification of cell-specific miRNA expression estimates. Further development will enhance rapid identification of cell-specific miRNA expression estimates in health and disease for nearly any cell type using archival FFPE material.

scholarly journals Whole Blood Holding Time Prior to Plasma Processing Alters microRNA Expression Profile

2022 ◽  
Vol 12 ◽  
Author(s):  
Sung Hye Kim ◽  
David A. MacIntyre ◽  
Lynne Sykes ◽  
Maria Arianoglou ◽  
Phillip R. Bennett ◽  
...  
Keyword(s):  
Mirna Expression ◽  
Whole Blood ◽  
Biomarker Discovery ◽  
Expression Profiles ◽  
Holding Time ◽  
Circulating Mirnas ◽  
Sample Collection ◽  
Aberrant Expression ◽  
Discovery Research ◽  
Mirna Expression Profiles

MicroRNAs (miRNAs) can exhibit aberrant expression under different physiological and pathological conditions. Therefore, differentially expressed circulating miRNAs have been a focus of biomarker discovery research. However, the use of circulating miRNAs comes with challenges which may hinder the reliability for their clinical application. These include varied sample collection protocols, storage times/conditions, sample processing and analysis methods. This study focused on examining the effect of whole blood holding time on the stability of plasma miRNA expression profiles. Whole blood samples were collected from healthy pregnant women and were held at 4°C for 30 min, 2 h, 6 h or 24 h prior to processing for plasma isolation. Plasma RNA was extracted and the expression of 179 miRNAs were analyzed. Unsupervised principal component analysis demonstrated that whole blood holding time was a major source of variation in miRNA expression profiles with 53 of 179 miRNAs showing significant changes in expression. Levels of specific miRNAs previously reported to be associated with pregnancy-associated complications such as hsa-miR-150-5p, hsa-miR-191-5p, and hsa-miR-29a-3p, as well as commonly used endogenous miRNA controls, hsa-miR-16-5p, hsa-miR-25-3p, and hsa-miR-223-3p were significantly altered with increase in blood holding time. Current protocols for plasma-based miRNA profiling for diagnostics describe major differences in whole blood holding periods ranging from immediately after collection to 26 h after. Our results demonstrate holding time can have dramatic effects on analytical reliability and reproducibility. This highlights the importance of standardization of blood holding time prior to processing for plasma in order to minimize introduction of non-biological variance in miRNA profiles.

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