scholarly journals Genetic analysis ofTP53gene mutations in exon 4 and exon 8 among esophageal cancer patients in Sudan

2019 ◽  
Author(s):  
Sulafa Mohamed Eltaher ◽  
Abeer Babiker Idris ◽  
A. H Mahmoud ◽  
Mawadah Yousif Mohamed Yousif ◽  
Nouh Saad Mohamed ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Cancer Patients ◽  
P53 Protein ◽  
Periodic Acid ◽  
Silent Mutation ◽  
Large Sample Size ◽  
P53 Codon 72 ◽  
Periodic Acid Schiff ◽  
Fixed Tissue ◽  
Exon 4

AbstractBackgroundEsophageal carcinoma (EC) represents the 1strank among all gastrointestinal cancers in Sudan. Despite little publications, there is a deep absence of literature about the molecular pathogenesis of EC considering TP53 gene from Sudanese population.AimsIn this study, we performed the expression analysis on p53 protein level by immunohistochemical staining and examined its overexpression with p53 mutations in exons 4 and 8 among esophageal cancer patients in Sudan.Material and MethodsFixed tissue with 10% buffered formalin was stained by Hematoxlin and Eosin (H&E), Alcian blue-Periodic Acid Schiff (PAS) and Immunohistochemistry stain. PCR-RFLP was used to study the frequencies of p53 codon 72 R/P polymorphism. Conventional PCR and sanger sequencing were applied for exon 4 and exon 8. Then detection and functional analysis of SNPs and mutations were performed using various in bioinformatics tools.ResultNuclear accumulations for p53 protein was detected in all of the esophageal carcinomas examined while no accumulations were observed in normal control sections. Four patients with immune-positive for p53 showed no mutations in p53 gene (exon4 and exon8). The incidence of the homozygous mutant variant Pro/Pro was higher in esophageal cancerous patients comparing to healthy control subject 20(71. 4%) vs. 1(10%), respectively (p=0.0026). In exon 4, no mutation was detected other than NG_017013.2:g. 16397C>G. While in exon 8, g.18783-18784AG>TT, g.18803A>C, g.18860A>C, g.18845A>T and g.18863_ 18864 InsT were observed.Conclusionwe found a significant association between the overexpression of TP53 protein and mutation in exon 4 and 8. A silent mutation P301P was detected in all of examined cases. Two patients who diagnosed with small cell sarcoma have shared the same mutations in exon8. Further studies with large sample size are required to demonstrate the usefulness of these mutations in the screening of EC especially SCCE.

scholarly journals FACTORS AFFECTING ENZYMATIC REMOVAL OF GLYCOGEN FROM TISSUE SECTIONS AND FROM ENTIRE RAT LIVER BLOCKS

1965 ◽  
Vol 13 (6) ◽  
pp. 488-497 ◽  
Author(s):  
RONALD D. PAEGLE
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rat Liver ◽  
Sodium Phosphate ◽  
Periodic Acid ◽  
Distilled Water ◽  
Periodic Acid Schiff ◽  
Fixed Tissue ◽  
Factors Affecting ◽  
Tissue Sections ◽  
Different Types

The rates of enzymatic glycogen removal from two different types of rat liver preparations were determined histochemically, employing the periodic acid-Schiff method to visualize glycogen. The first preparation consisted of elongated liver blocks (approximately 2 x 2 x 10 mm) which were fixed, then exposed to the alpha amylases before dehydration and embedding. The second type of preparation consisted of 5-8 µ thick, routinely prepared sections. The glycogen was removed considerably slower from the formaldehyde fixed tissue blocks than from the sections. The rate of glycogen hydrolysis was enchanced in the liver blocks by brief periods of autolysis, by removal of lipids and by washing before exposure to the enzyme. Hydrolysis by amylase was slower when 0.18 M phosphate was used with the formaldehyde during fixation than when veronal acetate or 0.018 M phosphate buffers were used at the same pH 7.2-7.4. Many peripherally located cells retained glycogen even after incubation of the entire blocks for 24 hours in alpha amylase when the buffer during fixation was 0.18 M sodium Phosphate. The contaminating ribonuclease present in malt diastase preparations penetrated readily into the cells of the elongated liver blocks but the protease affected only the periphery during the 24 hour incubation. The differences in resistance to enzymatic hydrolysis were not confined to the tissue blocks. The type of buffer and fixative used for tissue fixation determined the subsequent susceptibility of glycogen to hydrolysis in sections also. The list is arranged in order of progressively decreasing resistance to enzymatic hydrolysis: 3.8% formaldehyde or 3% glutaraldehyde with 0.18 M phosphate, 3.8% formaldehyde with 0.018 M phosphate or 0.18 M veronal acetate, 3% glutaraldehyde with distilled water, 3.8% formaldehyde with 0.129 M veronal acetate or distilled water, and 70-95% ethanol. In addition, glycogen was removed slower from the peripheral zones of the sections than from the central portions.

Incidence of a split alpha 2-glycoprotein band in the electrophoretic pattern for serum of adenocarcinoma patients.

1980 ◽  
Vol 26 (3) ◽  
pp. 392-395 ◽  
Author(s):  
G B Dermer ◽  
L M Silverman ◽  
S J Gendler ◽  
Z A Tökés
Keyword(s):  
Cancer Patients ◽  
Serum Proteins ◽  
Acute Phase Proteins ◽  
Systemic Disease ◽  
Periodic Acid ◽  
Electrophoretic Pattern ◽  
Control Group ◽  
Periodic Acid Schiff ◽  
Cellulose Acetate Membranes ◽  
Schiff Reagent

Abstract We electrophoresed serum samples on Mylar-backed cellulose acetate membranes and stained for glycoproteins with the periodic acid—Schiff reagent. The samples were from untreated adenocarcinoma patients, adenocarcinoma patients receiving chemotherapy, and patients with other malignancies, and also from patients with benign proliferative diseases, inflammatory diseases, and other non-malignant conditions. Forty-five per cent of the sera from untreated adenocarcinoma patients and 80% of those from adenocarcinoma patients with progressive systemic disease exhibited a splitting of the alpha 2-glycoproteins into a fast and slow band. Such a pattern was seen in only 4% of the non-adenocarcinoma cancer patients and 4% of the control group. Serial studies indicated that electrophoretic patterns of alpha 2-glycoproteins change with clinical status. Non-cancer patients with high concentrations of acute-phase proteins in their serum did not exhibit two alpha 2-glycoprotein bands. Further characterization of serum proteins from the fast alpha 2 region suggest that alpha 1-acid glycoprotein and haptoglobin beta chains are the principal components staining with periodic acid—Schiff reagent. These components are markedly less apparent in, or are absent from, the fast alpha 2 region of normal sera.

Incidence of a split alpha 2-glycoprotein band in the electrophoretic pattern for serum of adenocarcinoma patients.

1980 ◽  
Vol 26 (3) ◽  
pp. 392-395 ◽  
Author(s):  
G B Dermer ◽  
L M Silverman ◽  
S J Gendler ◽  
Z A Tökés
Keyword(s):  
Cancer Patients ◽  
Serum Proteins ◽  
Acute Phase Proteins ◽  
Systemic Disease ◽  
Periodic Acid ◽  
Electrophoretic Pattern ◽  
Control Group ◽  
Periodic Acid Schiff ◽  
Cellulose Acetate Membranes ◽  
Schiff Reagent

Abstract We electrophoresed serum samples on Mylar-backed cellulose acetate membranes and stained for glycoproteins with the periodic acid—Schiff reagent. The samples were from untreated adenocarcinoma patients, adenocarcinoma patients receiving chemotherapy, and patients with other malignancies, and also from patients with benign proliferative diseases, inflammatory diseases, and other non-malignant conditions. Forty-five per cent of the sera from untreated adenocarcinoma patients and 80% of those from adenocarcinoma patients with progressive systemic disease exhibited a splitting of the alpha 2-glycoproteins into a fast and slow band. Such a pattern was seen in only 4% of the non-adenocarcinoma cancer patients and 4% of the control group. Serial studies indicated that electrophoretic patterns of alpha 2-glycoproteins change with clinical status. Non-cancer patients with high concentrations of acute-phase proteins in their serum did not exhibit two alpha 2-glycoprotein bands. Further characterization of serum proteins from the fast alpha 2 region suggest that alpha 1-acid glycoprotein and haptoglobin beta chains are the principal components staining with periodic acid—Schiff reagent. These components are markedly less apparent in, or are absent from, the fast alpha 2 region of normal sera.

scholarly journals Clinico-morphological Profile of Cutaneous Fungal Infections: An Experience from a Tertiary Care Government Hospital in North India

2021 ◽  
Vol 8 (2) ◽  
pp. A56-62
Author(s):  
Ekta Jain ◽  
Rajpal Singh Punia ◽  
Jagdish Chander ◽  
Mala Bhalla
Keyword(s):  
Fungal Infections ◽  
Periodic Acid ◽  
Critical Role ◽  
Tertiary Care ◽  
Rapid Identification ◽  
Histochemical Staining ◽  
North India ◽  
Fungal Culture ◽  
Periodic Acid Schiff ◽  
Fixed Tissue

Background: Cutaneous fungal infections are predominantly seen in hot tropical countries like India. In the past decade, there has been an escalation in recurrent and chronic fungal infections. Skin biopsy may play a critical role in rapid identification of these infections. Methods: Fifteen cases of cutaneous fungal infections over a period of 6 years were included. Formalin-fixed tissue was subjected to hematoxylin and eosin and histochemical staining including Gomori Methanamine Silver and Periodic Acid Schiff . Results of KOH smear test and fungal culture were included wherever available. The clinico-morphological patterns in various cutaneous fungal infections was evaluated. Results: Most patients were between 25 to 40 years of age and commonly presented as skin ulceration, followed by nodular swelling and multiple discharging sinuses. Candidiasis and Mycetoma infections were the commonest infections. Others included: Cryptococcosis, Dermatophytosis, Chromoblastomycosis and Mucormycosis. The predominant histopathologic patterns were perivascular and interstitial inflammation. Eleven cases were confirmed by KOH examination and culture. Conclusion: The morphologic spectrum of cutaneous fungal infections is varied. Initial presentations of these fungal infections may be indicative of the onset of a life-threatening systemic mycoses. Thus, the histopathologic evaluation of skin tissue specimens is critical for their rapid and accurate diagnosis.

scholarly journals THE EFFECT OF SAPONIFICATION UPON CERTAIN HISTOCHEMICAL REACTIONS OF THE EPITHELIAL MUCINS OF THE GASTROINTESTINAL TRACT

1971 ◽  
Vol 19 (11) ◽  
pp. 654-662 ◽  
Author(s):  
C. F. A. CULLING ◽  
P. E. REID ◽  
W. L. DUNN
Keyword(s):  
Gastrointestinal Tract ◽  
Guinea Pig ◽  
Periodic Acid ◽  
Ileocecal Valve ◽  
Paraffin Sections ◽  
Periodic Acid Schiff ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Cryostat Sections ◽  
Formalin Fixed

An increase in periodic acid-Schiff reactivity after saponification has been demonstrated in the gastrointestinal tract mucins of man, rat, rabbit and guinea pig. Tins pH-dependent phenomenon is independent of fixation and processing since it occurred in cryostat sections of fresh and formalin-fixed tissue and in paraffin sections of tissues fixed in eight different fixatives. In man and rat this effect occurs at the level of the ileocecal valve and below; in guinea pig it is confined to the large intestine and rectum; while in rabbits it occurs in Brunner's glands, the ileocecal valve and below. In man the increase is found throughout the crypts whereas in rabbits the effect is seen mainly at the luminal end; in rats it is seen largely at the base of the crypts; and in guinea pigs it is variable. This effect, due to an increase in the number of 1:2 glycol groups, is accompanied in man, rat and guinea pig by an increase in basophilia due to the presence of sulfate and/or other highly acidic groups.

Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

1977 ◽  
Vol 35 ◽  
pp. 640-641
Author(s):  
J. R. Ruby
Keyword(s):  
Endoplasmic Reticulum ◽  
Parotid Gland ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Duct System ◽  
Parotid Glands ◽  
Dasypus Novemcinctus ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

Detection of Carriers in Glanzmann's Thrombasthenia

1983 ◽  
Vol 49 (03) ◽  
pp. 182-186
Author(s):  
G T E Zonneveld ◽  
E F van Leeuwen ◽  
A Sturk ◽  
J W ten Cate
Keyword(s):  
Bleeding Time ◽  
Periodic Acid ◽  
Type I ◽  
Clot Retraction ◽  
Periodic Acid Schiff ◽  
Glanzmann’S Thrombasthenia ◽  
Aggregation Response ◽  
Glanzmann's Thrombasthenia ◽  
Sds Page ◽  
Reduced State

SummaryQuantitative glycoprotein (GP) analysis of whole platelets or platelet membranes was performed by SDS-polyacrylamide gelelectrophoresis (SDS-PAGE) and periodic acid Schiff staining in the families of two unrelated Glanzmann’s thrombasthenia (GT) patients. Each family consisted of two symptom free parents, a symptom free daughter and a GT daughter. All symptom free members had a normal bleeding time, clot retraction and platelet aggregation response to adenosine 5’-diphosphate (ADP), collagen and adrenalin. Platelet Zw* antigen was normally expressed in these subjects. GT patiens, classified as a type I and II subject, showed reduced amounts of GP lib and of GP nia. Analysis of isolated membranes in the non-reduced state, however, showed that the amount of GP Ilia was also reduced in three of the four parents, whereas one parent (of the GT type I patient) and the two unaffected daughters had normal amounts of GP Ilia. Quantitative SDS-PAGE may therefore provide a method for the detection of asymptomatic carriers in GT type I and II.

Swim bladder mycosis in farmed rainbow trout Oncorhynchus mykiss caused by Phoma herbarum and experimental verification of pathogenicity

2020 ◽  
Vol 138 ◽  
pp. 237-246 ◽  
Author(s):  
J Řehulka ◽  
A Kubátová ◽  
V Hubka
Keyword(s):  
Rainbow Trout ◽  
Histopathological Examination ◽  
Periodic Acid ◽  
Bladder Wall ◽  
Swim Bladder ◽  
Cell Reactivity ◽  
Periodic Acid Schiff ◽  
Posterior Portion ◽  
Hepatic Congestion ◽  
Phoma Herbarum

In this study, spontaneous swim bladder mycosis was documented in a farmed fingerling rainbow trout from a raceway culture system. At necropsy, the gross lesions included a thickened swim bladder wall, and the posterior portion of the swim bladder was enlarged due to massive hyperplasia of muscle. A microscopic wet mount examination of the swim bladder contents revealed abundant septate hyphae, and histopathological examination showed periodic acid-Schiff-positive mycelia in the lumen and wall of the swim bladder. Histopathological examination of the thickened posterior swim bladder revealed muscle hyperplasia with expansion by inflammatory cells. The causative agent was identified as Phoma herbarum through morphological analysis and DNA sequencing. The disease was reproduced in rainbow trout fingerlings using intraperitoneal injection of a spore suspension. Necropsy in dead and moribund fish revealed extensive congestion and haemorrhages in the serosa of visceral organs and in liver and abdominal serosanguinous fluid. Histopathological examination showed severe hepatic congestion, sinusoidal dilatation, Kupffer cell reactivity, leukostasis and degenerative changes. Fungi were disseminated to the liver, pyloric caeca, kidney, spleen and heart. Although infections caused by Phoma spp. have been repeatedly reported in fish, species identification has been hampered by extensive taxonomic changes. The results of this study confirmed the pathogenicity of P. herbarum in salmonids by using a reliably identified strain during experimental fish infection and provides new knowledge regarding the course of infection.

Erdheim-Chester Disease Presenting with Histiocytic Colitis and Cytokine Storm

2017 ◽  
Vol 26 (2) ◽  
pp. 183-187
Author(s):  
George P. Christophi ◽  
Yeshika Sharma ◽  
Quader Farhan ◽  
Umang Jain ◽  
Ted Walker ◽  
...  
Keyword(s):  
Periodic Acid ◽  
Poor Response ◽  
Rare Type ◽  
Periodic Acid Schiff ◽  
Hematopoietic Stem ◽  
Hemodynamic Compromise ◽  
Erdheim Chester Disease ◽  
Erdheim Chester ◽  
Chester Disease ◽  
Segmental Colitis

Background: Non-Langerhans histiocytosis is a group of inflammatory lymphoproliferative disorders originating from non-clonal expansion of hematopoietic stem cells into cytokine-secreting dendritic cells or macrophages. Erdheim-Chester Disease (ECD) is a rare type of non-Langerhans cell histiocytosis characterized by tissue inflammation and injury caused by macrophage infiltration and histologic findings of foamy histiocytes. Often ECD involves the skeleton, retroperitoneum and the orbits. This is the first report documenting ECD manifesting as segmental colitis and causing cytokine-release syndrome.Case presentation: A 68-year old woman presented with persistent fever without infectious etiology and hematochezia. Endoscopy showed segmental colitis and pathology revealed infiltration of large foamy histiocytes CD3-/CD20-/CD68+/CD163+/S100- consistent with ECD. The patient was empirically treated with steroids but continued to have fever and developed progressive distributive shock.Conclusion: This case report describes the differential diagnosis of infectious and immune-mediated inflammatory and rheumatologic segmental colitis. Non-Langerhans histiocytosis and ECD are rare causes of gastrointestinal inflammation. Prompt diagnosis is imperative for the appropriate treatment to prevent hemodynamic compromise due to distributive shock or gastrointestinal bleeding. Importantly, gastrointestinal ECD might exhibit poor response to steroid treatment and other potential treatments including chemotherapy, and biologic treatments targeting IL-1 and TNF-alpha signaling should be considered.Abbreviations: AFB: acid-fast bacilli; ECD: Erdheim-Chester Disease; IBD: inflammatory bowel disease; PASD: periodic acid-Schiff with diastase; TB: tuberculosis

Sign in / Sign up

Export Citation Format

Share Document