scholarly journals Shotgun proteomics analysis of nanoparticle-synthesisingDesulfovibrio alaskensisin response to platinum and palladium

2019 ◽  
Author(s):  
Michael J. Capeness ◽  
Lisa Imrie ◽  
Lukas F. Mühlbauer ◽  
Thierry Le Bihan ◽  
Louise E. Horsfall
Keyword(s):  
Resistance Mechanism ◽  
Shotgun Proteomics ◽  
Small Subunit ◽  
Industrial Applications ◽  
Metal Resistance ◽  
Platinum Group Metals ◽  
System A ◽  
Genetic Components ◽  
Stress Response System ◽  
Contaminant Metals

AbstractPlatinum and palladium are much sought-after metals of global critical importance in terms of abundance and availability. At the nano-scale these metals are of even higher value due to their catalytic abilities for industrial applications.Desulfovibrio alaskensisis able to capture ionic forms of both of these metals, reduce them, and synthesize elemental nanoparticles. Despite this ability very little is known about the biological pathways involved in the formation of these nanoparticles. Proteomic analysis ofD. alaskensisin response to platinum and palladium has highlighted those proteins involved in both the reductive pathways and the wider stress-response system. A core set of 13 proteins was found in both treatments and consisted of proteins involved in metal transport and reduction. There were also 7 proteins specific to either platinum or palladium. Over-expression of one of these platinum-specific genes, a NiFe hydrogenase small subunit (Dde_2137), resulted in the formation of larger nanoparticles. This study improves our understanding of the pathways involved in the metal resistance mechanism ofDesulfovibrioand informs how we can tailor the bacterium for nanoparticle production, enhancing its application as a bioremediation tool and as way to capture contaminant metals from the environment.ImportanceBacteria, in particularlyD. alaskensis, represent a biological and greener way to capture high value metals such as platinum group metals from environmental and industrial waste streams. The recovery of these metals in nanoparticle forms adds extra value to this process as they can be used in a variety of different industrial applications as they have exceptional catalytic capabilities.D. alaskensisability to do this, has been widely reported, though very little is understood about the underlying protein and genetic components. It is by understanding the biological basis of this capability that we can further improve and adapt this bacterium to be better at bioremediation and to control its ability to do so.

scholarly journals Genome-Driven Discovery of Enzymes with Industrial Implications from the Genus Aneurinibacillus

2021 ◽  
Vol 9 (3) ◽  
pp. 499
Author(s):  
Majid Rasool Kamli ◽  
Nada A. Y. Alzahrani ◽  
Nahid H. Hajrah ◽  
Jamal S. M. Sabir ◽  
Adeel Malik
Keyword(s):  
Heavy Metal ◽  
Enzyme Activities ◽  
Heavy Metal Resistance ◽  
Industrial Applications ◽  
Metal Resistance ◽  
The Family ◽  
Antiviral Activities ◽  
Genome Level ◽  
Genome Analyses ◽  
Unique Species

Bacteria belonging to the genus Aneurinibacillus within the family Paenibacillaceae are Gram-positive, endospore-forming, and rod-shaped bacteria inhabiting diverse environments. Currently, there are eight validly described species of Aneurinibacillus; however, several unclassified species have also been reported. Aneurinibacillus spp. have shown the potential for producing secondary metabolites (SMs) and demonstrated diverse types of enzyme activities. These features make them promising candidates with industrial implications. At present, genomes of 9 unique species from the genus Aneurinibacillus are available, which can be utilized to decipher invaluable information on their biosynthetic potential as well as enzyme activities. In this work, we performed the comparative genome analyses of nine Aneurinibacillus species representing the first such comprehensive study of this genus at the genome level. We focused on discovering the biosynthetic, biodegradation, and heavy metal resistance potential of this under-investigated genus. The results indicate that the genomes of Aneurinibacillus contain SM-producing regions with diverse bioactivities, including antimicrobial and antiviral activities. Several carbohydrate-active enzymes (CAZymes) and genes involved in heavy metal resistance were also identified. Additionally, a broad range of enzyme classes were also identified in the Aneurinibacillus pan-genomes, making this group of bacteria potential candidates for future investigations with industrial applications.

scholarly journals Inorganic Polyphosphate, Exopolyphosphatase, andPho84-Like Transporters May Be Involved in Copper Resistance inMetallosphaera sedulaDSM 5348T

Archaea ◽  
2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Matías Rivero ◽  
Constanza Torres-Paris ◽  
Rodrigo Muñoz ◽  
Ricardo Cabrera ◽  
Claudio A. Navarro ◽  
...  
Keyword(s):  
Present Report ◽  
Resistance Mechanism ◽  
Metal Resistance ◽  
Copper Resistance ◽  
Site Directed Mutagenesis ◽  
Pcr Analysis ◽  
Linear Polymers ◽  
Inorganic Polyphosphate ◽  
Metallosphaera Sedula

Polyphosphates (PolyP) are linear polymers of orthophosphate residues that have been proposed to participate in metal resistance in bacteria and archaea. In addition of having a CopA/CopB copper efflux system, the thermoacidophilic archaeonMetallosphaera sedulacontains electron-dense PolyP-like granules and a putative exopolyphosphatase (PPXMsed,Msed_0891) and four presumedpho84-like phosphate transporters (Msed_0846,Msed_0866,Msed_1094, andMsed_1512) encoded in its genome. In the present report, the existence of a possible PolyP-based copper-resistance mechanism inM. sedulaDSM 5348Twas evaluated.M. sedulaDSM 5348Taccumulated high levels of phosphorous in the form of granules, and its growth was affected in the presence of 16 mM copper. PolyP levels were highly reduced after the archaeon was subjected to an 8 mM CuSO4shift. PPXMsedwas purified, and the enzyme was found to hydrolyze PolyPin vitro. Essential residues for catalysis of PPXMsedwere E111 and E113 as shown by a site-directed mutagenesis of the implied residues. Furthermore,M. sedula ppx,pho84-like, andcopTMAgenes were upregulated upon copper exposure, as determined by qRT-PCR analysis. The results obtained support the existence of a PolyP-dependent copper-resistance system that may be of great importance in the adaptation of this thermoacidophilic archaeon to its harsh environment.

Condenser Tube Examination Using Acoustic Pulse Reflectometry

2008 ◽  
Author(s):  
Noam Amir ◽  
Oded Barzelay ◽  
Amir Yefet ◽  
Tal Pechter
Keyword(s):  
Acoustic Pulse ◽  
Large Degree ◽  
Industrial Applications ◽  
Detection Methods ◽  
Internal Diameter ◽  
Condenser Tube ◽  
Indirect Methods ◽  
Wave Separation ◽  
System A ◽  
Propagating Waves

Acoustic Pulse Reflectometry (APR) has been applied extensively to tubular systems in research laboratories, for purposes of measuring input impedance, bore reconstruction, and fault detection. Industrial applications have been mentioned in the literature, though they have not been widely implemented. Academic APR systems are extremely bulky, often employing source tubes of six meters in length, which limits their industrial use severely. Furthermore, leak detection methods described in the literature are based on indirect methods, by carrying out bore reconstruction and finding discrepancies between the expected and reconstructed bore. In this paper we describe an APR system designed specifically for detecting faults commonly found in industrial tube systems: leaks, increases in internal diameter caused by wall thinning, and constrictions. The system employs extremely short source tubes, on the order of 20cm, making it extremely portable, but creating a large degree of overlap between forward and backward propagating waves in the system. A series of algorithmic innovations enable the system to perform the wave separation mathematically, and then identify the above faults automatically, with a measurement time on the order of 10 seconds per tube. We present several case studies of condenser tube inspection, showing how different faults are identified and reported.

scholarly journals Tuning and Feasibility Analysis of Classical First-Order MIMO Non-Linear Sliding Mode Control Design for Industrial Applications

Machines ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 10 ◽  
Author(s):  
Alessandro Palmieri ◽  
Renato Procopio ◽  
Andrea Bonfiglio ◽  
Massimo Brignone ◽  
Marco Invernizzi ◽  
...  
Keyword(s):  
Feedback Linearization ◽  
Sliding Mode ◽  
Parameter Tuning ◽  
Industrial Applications ◽  
Single Input Single Output ◽  
First Order ◽  
Control Techniques ◽  
Single Output ◽  
System A ◽  
Single Input

Model-based control techniques have been gaining more and more interest these days. These complex control systems are mostly based on theories, such as feedback linearization, model predictive control, adaptive and robust control. In this paper the latter approach is investigated, in particular, sliding mode (SM) control is analyzed. While several works on the description and application of SM control on single-input single-output systems can easily be found, its application on multi-input multi-output systems is not examined in depth at the same level. Hence, this work aims at formalizing some theoretical complements about the necessary conditions for the feasibility of the SM control for multi-input-multi-output systems. Furthermore, in order to obtain the desired performance from the control system, a method for parameter tuning is proposed in the particular case in which the relative degree of the controlled channels is equal to one. Finally, a simple control problem example is shown with the aim of stressing the benefits derived from the application of the theoretical complements described here.

Internal kinematics in a planar granular column collapse

2020 ◽  
Author(s):  
Miguel Angel Cabrera ◽  
Gustavo Pinzón
Keyword(s):  
Complex Dynamics ◽  
Industrial Applications ◽  
Mass Movements ◽  
System A ◽  
Column Collapse ◽  
Fluid Systems ◽  
Gravity Driven ◽  
Deformation Patterns ◽  
Granular Column ◽  
Fluid Interactions

<p>The granular column collapse is a simplified system of the complex dynamics observed in gravity-driven natural mass-movements (i.e., landslides, debris flows, rock avalanches) and industrial applications (i.e., pharmaceutics, concrete, and food industry). In this system, a granular column is built with an initial height and initial width and then is allowed to collapse by self-weight onto a horizontal plane, while observing the variation in runout as a function of its initial geometry. Despite its wide use in the study of mass-movements mobility, either dry or with a liquid, little is known on the internal physics during collapse and its variation when immersed in an ambient fluid. This work presents a planar setup that allows the study of fully and partially immersed granular columns, with little disturbance at release [1]. The use of a planar configuration allows the monitoring of the moving mass and its deformation patterns, providing a unique insight into the particle-fluid interactions at release and during collapse that were not possible before. These observations are of great importance for the understanding of particle-fluid interactions at a mesoscale and can shed light into larger processes like a submarine and subaerial landslides. This work addresses these interactions by varying the geometry and measuring the mobility in dry and immersed conditions. The associated deformation patterns are observed both at the column-scale and at the particle-scale, reflecting in the velocity scaling of a deformable and moving granular mass and the occasional ejection of particles at its surface. We observed that the area of the released portion decreases during collapse and converges toward an equivalent portion of surface particles with little influence by the initial column geometry. These observations validate the planar setup for the study of granular columns, provides a novel interpretation in the momentum transfer in particle-fluid systems, and sets a validation case for future numerical simulations.</p><p>[1] Pinzon & Cabrera, Planar collapse of a submerged granular column. Physics of fluids, v31, 2019.</p>

CONTENT BASED IMAGE RETRIEVAL USING DISCRETE WAVELET TRANSFORM

2004 ◽  
Vol 18 (01) ◽  
pp. 19-32 ◽  
Author(s):  
SAEID BELKASIM ◽  
XIANYU HONG ◽  
O. BASIR
Keyword(s):  
Wavelet Transform ◽  
Image Retrieval ◽  
Discrete Wavelet Transform ◽  
Retrieval System ◽  
Industrial Applications ◽  
Discrete Wavelet ◽  
Retrieval Process ◽  
System A ◽  
Image Transforms ◽  
The Common

Image retrieval plays an important role in a broad spectrum of applications. Contentbased retrieval (CBR) is one of the popular choices in many biomedical and industrial applications. Discrete image transforms have been widely studied and suggested for many image retrieval applications. The Discrete Wavelet Transform (DWT) is one of the most popular transforms recently applied to many image processing applications. The Daubechies wavelet can be used to form the basis for extracting features in retrieving images based on the description of a particular object within the scene. This wavelet is widely used for image compression. In this paper we highlight the common features between compression and retrieval. Several examples are used to test the DWT retrieval system. A comparison between DWT and Discrete Cosine Transform (DCT) is also made. The retrieval system using DWT requires preprocessing and normalization of images, which might slow down the retrieval process. The accuracy of the retrieval using DWT has been significantly improved by incorporating efficient K-Neighbor Nearest Distance (KNND) measure in our system.

scholarly journals Modelling & Position Control for Electro-Hydraulic System

2019 ◽  
Vol 8 (4) ◽  
pp. 3841-3845 ◽  
Keyword(s):  
Control System ◽  
Hydraulic System ◽  
Position Control ◽  
Industrial Applications ◽  
Hydraulic Systems ◽  
System A ◽  
Complex Process ◽  
Highly Nonlinear ◽  
Back Stepping Control ◽  
And Control

Electro-hydraulic systems (EHS) are widely used in industrial applications due to the high-power density and accuracy. However, EHS are highly nonlinear which makes its modelling and control aspects a complex process. In this paper, we present the modelling and position control for an electro-hydraulic system (EHS). The mathematical modelling is carried out considering the non-linearities like friction, discharge coefficient and load mass present in the system. A back-stepping control scheme is developed for maintaining the accuracy in the position control. The closed-loop stability of the proposed control system is analyzed with Lyapunov’s theory. The performance of the control system under the effect of bounded external uncertainties is validated with simulation study. The study indicates that the proposed controller gives an effective motion control in presence of the system uncertainties.

Condenser Tube Examination Using Acoustic Pulse Reflectometry

2009 ◽  
Vol 132 (1) ◽  
Author(s):  
N. Amir ◽  
O. Barzelay ◽  
A. Yefet ◽  
T. Pechter
Keyword(s):  
Acoustic Pulse ◽  
Large Degree ◽  
Industrial Applications ◽  
Detection Methods ◽  
Internal Diameter ◽  
Condenser Tube ◽  
Indirect Methods ◽  
Wave Separation ◽  
System A ◽  
Propagating Waves

Acoustic pulse reflectometry (APR) has been applied extensively to tubular systems in research laboratories for purposes of measuring input impedance, bore reconstruction, and fault detection. Industrial applications have been mentioned in the literature, though they have not been widely implemented. Academic APR systems are extremely bulky, often employing source tubes of 6 m in length, which limits their industrial use severely. Furthermore, leak detection methods described in the literature are based on indirect methods, by carrying out bore reconstruction and finding discrepancies between the expected and reconstructed bore. In this paper we describe an APR system designed specifically for detecting faults commonly found in industrial tube systems: leaks, increases in internal diameter caused by wall thinning, and constrictions. The system employs extremely short source tubes, in the order of 20 cm, making it extremely portable, but creating a large degree of overlap between forward and backward propagating waves in the system. A series of algorithmic innovations enable the system to perform the wave separation mathematically, and then identify the above faults automatically with a measurement time on the order of 10 s per tube. We present several case studies of condenser tube inspection, showing how different faults are identified and reported.

scholarly journals The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, Pollicipes pollicipes

2020 ◽  
Vol 21 (7) ◽  
pp. 2524
Author(s):  
Dany Domínguez-Pérez ◽  
Daniela Almeida ◽  
Josef Wissing ◽  
André M. Machado ◽  
Lothar Jänsch ◽  
...  
Keyword(s):  
Protein Composition ◽  
Industrial Applications ◽  
Rocky Shores ◽  
Label Free ◽  
System A ◽  
Goose Barnacle ◽  
Pollicipes Pollicipes ◽  
Transcriptomic Level ◽  
Adhesive Proteins ◽  
And Function

Adhesive secretion has a fundamental role in barnacles’ survival, keeping them in an adequate position on the substrate under a variety of hydrologic regimes. It arouses special interest for industrial applications, such as antifouling strategies, underwater industrial and surgical glues, and dental composites. This study was focused on the goose barnacle Pollicipes pollicipes adhesion system, a species that lives in the Eastern Atlantic strongly exposed intertidal rocky shores and cliffs. The protein composition of P. pollicipes cement multicomplex and cement gland was quantitatively studied using a label-free LC-MS high-throughput proteomic analysis, searched against a custom transcriptome-derived database. Overall, 11,755 peptide sequences were identified in the gland while 2880 peptide sequences were detected in the cement, clustered in 1616 and 1568 protein groups, respectively. The gland proteome was dominated by proteins of the muscle, cytoskeleton, and some uncharacterized proteins, while the cement was, for the first time, reported to be composed by nearly 50% of proteins that are not canonical cement proteins, mainly unannotated proteins, chemical cues, and protease inhibitors, among others. Bulk adhesive proteins accounted for one-third of the cement proteome, with CP52k being the most abundant. Some unannotated proteins highly expressed in the proteomes, as well as at the transcriptomic level, showed similar physicochemical properties to the known surface-coupling barnacle adhesive proteins while the function of the others remains to be discovered. New quantitative and qualitative clues are provided to understand the diversity and function of proteins in the cement of stalked barnacles, contributing to the whole adhesion model in Cirripedia.

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