scholarly journals Multi-allelic APRR2 Gene is Associated with Fruit Pigment Accumulation in Melon and Watermelon

2019 ◽  
Author(s):  
Elad Oren ◽  
Galil Tzuri ◽  
Lea Vexler ◽  
Asaf Dafna ◽  
Ayala Meir ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Qualitative Difference ◽  
Fruit Color ◽  
Color Variation ◽  
Consumer Acceptance ◽  
Haplotypic Diversity ◽  
Allelism Tests ◽  
Pigment Accumulation ◽  
Light Green

AbstractColor and pigment content are important aspects of fruit quality and consumer acceptance of cucurbit crops. Here, we describe the independent mapping and cloning of a common causative APRR2 gene regulating pigment accumulation in melon and watermelon. We initially show that the APRR2 transcription factor is causative for the qualitative difference between dark and light green rind in both crops. Further analyses establish the link between sequence or expression level variations in the CmAPRR2 gene and pigments content in the rind and flesh of mature melon fruits. GWAS of young fruit rind color in a panel composed of 177 diverse melon accessions did not result in any significant association, leading to an earlier assumption that multiple genes are involved in shaping the overall phenotypic variation at this trait. Through resequencing of 25 representative accessions and allelism tests between light rind accessions, we show that multiple independent SNPs in the CmAPRR2 gene are causative for the light rind phenotype. The multi-haplotypic nature of this gene explain the lack of detection power obtained through GBS-based GWAS and confirm the pivotal role of this gene in shaping fruit color variation in melon. This study demonstrates the power of combining bi- and multi-allelic designs with deep sequencing, to resolve lack of power due to high haplotypic diversity and low allele frequencies. Due to its central role and broad effect on pigment accumulation in fruits, the APRR2 gene is an attractive target for carotenoids bio-fortification of cucurbit crops.

scholarly journals The multi-allelic APRR2 gene is associated with fruit pigment accumulation in melon and watermelon

2019 ◽  
Vol 70 (15) ◽  
pp. 3781-3794 ◽  
Author(s):  
Elad Oren ◽  
Galil Tzuri ◽  
Lea Vexler ◽  
Asaf Dafna ◽  
Ayala Meir ◽  
...  
Keyword(s):  
Genome Wide Association Study ◽  
Qualitative Difference ◽  
Genotyping By Sequencing ◽  
Color Variation ◽  
Nucleotide Polymorphisms ◽  
Genome Wide ◽  
Haplotypic Diversity ◽  
A Genome ◽  
Allelism Tests ◽  
Pigment Accumulation

Abstract Color and pigment contents are important aspects of fruit quality and consumer acceptance of cucurbit crops. Here, we describe the independent mapping and cloning of a common causative APRR2 gene regulating pigment accumulation in melon and watermelon. We initially show that the APRR2 transcription factor is causative for the qualitative difference between dark and light green rind in both crops. Further analyses establish the link between sequence or expression level variations in the CmAPRR2 gene and pigment content in the rind and flesh of mature melon fruits. A genome-wide association study (GWAS) of young fruit rind color in a panel composed of 177 diverse melon accessions did not result in any significant association, leading to an earlier assumption that multiple genes are involved in shaping the overall phenotypic variation in this trait. Through resequencing of 25 representative accessions and allelism tests between light rind accessions, we show that multiple independent single nucleotide polymorphisms in the CmAPRR2 gene are causative of the light rind phenotype. The multi-haplotypic nature of this gene explains the lack of detection power obtained through genotyping by sequencing-based GWAS and confirms the pivotal role of this gene in shaping fruit color variation in melon. This study demonstrates the power of combining bi- and multi-allelic designs with deep sequencing, to resolve lack of power due to high haplotypic diversity and low allele frequencies. Due to its central role and broad effect on pigment accumulation in fruits, the APRR2 gene is an attractive target for carotenoid bio-fortification of cucurbit crops.

scholarly journals Phytoene Synthase 2 Can Compensate for the Absence of Psy1 in Pepper Fruit (Capsicum annuum)

2019 ◽  
Author(s):  
So-Jeong Jang ◽  
Hyo-Bong Jeong ◽  
Ayoung Jung ◽  
Min-Young Kang ◽  
Suna Kim ◽  
...  
Keyword(s):  
Fruit Color ◽  
Color Variation ◽  
Phytoene Synthase ◽  
Pcr Analysis ◽  
Virus Induced Gene Silencing ◽  
Knockout Mutant ◽  
Color Development ◽  
Yellow Color ◽  
Pepper Fruit

AbstractPhytoene synthase 1 (PSY1) and Capsanthin-capsorubin synthase (CCS) are two major genes responsible for fruit color variation in pepper (Capsicum spp.), although fruit colors cannot be explained by variations in these two genes alone. Furthermore, the role of PSY2 in fruit color development in pepper is unknown. Here, we used a systemic approach to discover the genetic factors responsible for the yellow fruit color of C. annuum ‘MicroPep Yellow’ (MY) and to reveal the role of PSY2 in fruit color. We detected a complete deletion of PSY1 and a retrotransposon insertion in CCS in MY. Despite the loss of PSY1 and CCS function, the MY and mutant F2 plants from a cross between MY and the MicroPep Red (MR) accumulated basal levels of carotenoids, indicating that other PSY genes may complement the loss of PSY1. A qRT-PCR analysis demonstrated that PSY2 is constitutively expressed in both MR and MY fruits, and a color complementation assay using Escherichia coli revealed that PSY2 is capable of biosynthesizing a carotenoid. Virus-induced gene silencing of PSY2 in MY resulted in white fruits. These findings suggest that PSY2 can compensate for the absence of PSY1 in fruit, resulting in the yellow color of MY fruits.HighlightWe reveal the novel function of PSY2 in the development of yellow pepper fruit coloration using a psy1 knockout mutant. This gene function was not previously identified in solanaceous crops.

scholarly journals Phytoene synthase 2 can compensate for the absence of PSY1 in the control of color in Capsicum fruit

2020 ◽  
Vol 71 (12) ◽  
pp. 3417-3427 ◽  
Author(s):  
So-Jeong Jang ◽  
Hyo-Bong Jeong ◽  
Ayoung Jung ◽  
Min-Young Kang ◽  
Suna Kim ◽  
...  
Keyword(s):  
Fruit Color ◽  
Color Variation ◽  
Phytoene Synthase ◽  
Pcr Analysis ◽  
Virus Induced Gene Silencing ◽  
Yellow Color ◽  
Pepper Fruit ◽  
Capsicum Spp ◽  
Complete Deletion

Abstract Phytoene synthase 1 (PSY1) and capsanthin-capsorubin synthase (CCS) are two major genes responsible for fruit color variation in pepper (Capsicum spp.). However, the role of PSY2 remains unknown. We used a systemic approach to examine the genetic factors responsible for the yellow fruit color of C. annuum ‘MicroPep Yellow’ (MY) and to determine the role of PSY2 in fruit color. We detected complete deletion of PSY1 and a retrotransposon insertion in CCS. Despite the loss of PSY1 and CCS function, both MY and mutant F2 plants from a cross between MY and the ‘MicroPep Red’ (MR) accumulated basal levels of carotenoids, indicating that other PSY genes may complement the loss of PSY1. qRT-PCR analysis indicated that PSY2 was constitutively expressed in both MR and MY fruits, and a color complementation assay using Escherichia coli revealed that PSY2 was capable of biosynthesizing a carotenoid. Virus-induced gene silencing of PSY2 in MY resulted in white fruits. These findings indicate that PSY2 can compensate for the absence of PSY1 in pepper fruit, resulting in the yellow color of MY fruits.

The role of transcription factor Egr‐1 in IL‐1 up‐regulation of tubular CD44 expression

Nephrology ◽  
2000 ◽  
Vol 5 (3) ◽  
pp. A92-A92
Author(s):  
Takazoe K ◽  
Foti R ◽  
Hurst La ◽  
Atkins Rc ◽  
Nikolic‐Paterson DJ.
Keyword(s):  
Transcription Factor ◽  
Cd44 Expression

Role of the transcription factor c-Jun/AP–1 in response to β-Catenin signaling in the liver

2012 ◽  
Vol 50 (01) ◽  
Author(s):  
C Trierweiler ◽  
K Willim ◽  
HE Blum ◽  
P Hasselblatt
Keyword(s):  
Transcription Factor ◽  
Factor C

1962-P: Role of Transcription Factor TCF21 in Adipocyte Progenitor Cells of Visceral Fat

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 1962-P
Author(s):  
TAKUYA MINAMIZUKA ◽  
YOSHIRO MAEZAWA ◽  
HARUHIDE UDAGAWA ◽  
YUSUKE BABA ◽  
MASAYA KOSHIZAKA ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Progenitor Cells ◽  
Visceral Fat

scholarly journals An R2R3-type myeloblastosis transcription factor MYB103 is involved in phosphorus remobilization

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Fangwei Yu ◽  
Shenyun Wang ◽  
Wei Zhang ◽  
Hong Wang ◽  
Li Yu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factor ◽  
Abiotic Stresses ◽  
Myb Transcription Factor ◽  
Ethylene Signaling ◽  
Biotic And Abiotic Stresses ◽  
P Deficiency ◽  
P Concentration ◽  
Increased Sensitivity

Abstract The members of myeloblastosis transcription factor (MYB TF) family are involved in the regulation of biotic and abiotic stresses in plants. However, the role of MYB TF in phosphorus remobilization remains largely unexplored. In the present study, we show that an R2R3 type MYB transcription factor, MYB103, is involved in phosphorus (P) remobilization. MYB103 was remarkably induced by P deficiency in cabbage (Brassica oleracea var. capitata L.). As cabbage lacks the proper mutant for elucidating the mechanism of MYB103 in P deficiency, another member of the crucifer family, Arabidopsis thaliana was chosen for further study. The transcript of its homologue AtMYB103 was also elevated in response to P deficiency in A. thaliana, while disruption of AtMYB103 (myb103) exhibited increased sensitivity to P deficiency, accompanied with decreased tissue biomass and soluble P concentration. Furthermore, AtMYB103 was involved in the P reutilization from cell wall, as less P was released from the cell wall in myb103 than in wildtype, coinciding with the reduction of ethylene production. Taken together, our results uncover an important role of MYB103 in the P remobilization, presumably through ethylene signaling.

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