scholarly journals Comparative transcriptome analysis of Powdery mildew Resistance between two Melon (Cucumis melo L) with Different Thickness Peel

2019 ◽  
Author(s):  
Cheng Hong ◽  
Kong Wei-ping ◽  
Lü Jun-Feng
Keyword(s):  
Powdery Mildew ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Rna Seq ◽  
Mildew Resistance ◽  
The World ◽  
Major Producer ◽  
Cucumis Melo L ◽  
Different Thickness ◽  
Powdery Mildew Infection

AbstractMelon (Cucumis melo L.) is wildly planted in the world and China is a major producer of muskmelon. Powdery mildew is one of the most common fungal diseases in the world and this disease frequently affects melon (Cucumis melo L.) and due to the reduction of melon yield. In this study, one material GanTianmi with thin peel and another material XueLianHua with thick peel were selected. After inoculating the powdery mildew, both materials were used to do the RNA-Seq. In total two RNA-seq libraries were constructed and sequenced separately. The reads per kilobase per Million mapped reads (RPKM) values of all the genes in the two materials were calculated and there were 13828 genes were expressed in the material G and 13944 genes were expressed in the material S (RPKM>1). The differentially expression gene (DEG) analysis result suggested that total 769 the DEGs between the two materials were identified. All the DEGs were annotated with several database and the transcript factors (TFs) that related to disease resistance such as MYB, ERF and WRKY among the DEGs were also identified. This research could not only provide the information about understanding the mechanism of powdery mildew infection but also help researchers breed the varieties with powdery mildew resistance.

scholarly journals Identification and Analysis of Powdery Mildew Resistance in Melon (Cucumis melo L.) Cultivar Meloni

BIOEDUSCIENCE ◽  
2020 ◽  
Vol 4 (1) ◽  
pp. 1-10
Author(s):  
Muhammad Alif Ishak ◽  
Budi Setiadi Daryono
Keyword(s):  
Powdery Mildew ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Morphological Characters ◽  
Lateral Part ◽  
Tolerance Level ◽  
Melon Seeds ◽  
Cucumis Melo L ◽  
Powdery Mildew Infection ◽  
Level Resistance

Background: A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. Meloni is a superior melon cultivar bred through a crossing between ♀ SL-3 and ♂ PI 371795, resulted by the Laboratory of Genetics and Breeding, Faculty of Biology, UGM. This study aimed to determine the level resistance of Meloni to powdery mildew infection and to identify the powdery mildew species that infected Meloni based on morphological characters. Methods: Meloni seeds were germinated and planted in the greenhouse of PIAT UGM. Powdery mildew spores were inoculated into the leaves after ±2 weeks of age. Leaf infected were scored using the gridline every 3 days for 6 weeks. Scoring results were converted to the diseases index score. Furthermore, powdery mildew species was identified using morphological characters. Results: Meloni had a tolerance level of resistance to the powdery mildew infection. Based on the morphological characters with fibrosin bodies, conidia ovoid-shape and the position of the germ tube in the lateral part of the conidia, powdery mildew that infected Meloni was expected as P. xanthii. Conclusions: Meloni can be expected as an alternative to superior melon seeds resistant to pest and disease infections especially powdery mildew.  

Codominant PCR-based markers and candidate genes for powdery mildew resistance in melon (Cucumis melo L.)

2011 ◽  
Vol 122 (4) ◽  
pp. 747-758 ◽  
Author(s):  
Fernando J. Yuste-Lisbona ◽  
Carmen Capel ◽  
María L. Gómez-Guillamón ◽  
Juan Capel ◽  
Ana I. López-Sesé ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Candidate Genes ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L

scholarly journals Identification and Expression Analysis of Fragaria Vesca MLO Genes Involved in Interaction with Powdery Mildew (Podosphaera Aphanis)

2017 ◽  
Vol 1 (1) ◽  
pp. 40-54 ◽  
Author(s):  
Shridhar Jambagi ◽  
Shridhar Jambagi ◽  
Jim M. Dunwell ◽  
Jim M. Dunwell
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Resistance Locus ◽  
Fragaria Vesca ◽  
Rna Seq ◽  
Mildew Resistance ◽  
Yield And Quality ◽  
Powdery Mildew Infection ◽  
Strawberry Cultivars ◽  
Diploid Strawberry

Strawberry powdery mildew, caused by Podosphaeraaphanis is a major fungal disease that affects strawberry yield and quality. In the model plant species Arabidopsis and the crop plants barley, tomato and pea, the Mildew resistance locus O (MLO) proteins have been found to be required for powdery mildew susceptibility. The present study, based on the sequence of a wild plum (Prunus americana) MLO protein, identified 16 MLO genes within the genome of woodland strawberry, Fragaria vesca and examined their expression pattern in response to powdery mildew infection in three diploid strawberry cultivars. Phylogenetic analysis showed that the FvMLO genes can be classified into six clades. Four FvMLO genes were grouped into clade III, which comprises MLO genes from Arabidopsis, tomato and grapevine that mediate powdery mildew susceptibility. A RNA-seq analysis of two diploid strawberry cultivars, F. vescassp. vesca accession Hawaii 4 (HW) and F. vesca f. semperflorens line “Yellow Wonder 5AF7” (YW) at 1 d (1 DAI) and 8 d (8 DAI) after infection showed the expression of 12 out of the 16 FvMLO genes. The comparison of Fragments Per Kilobase of transcript per Million mapped reads (FPKM values) detected by RNA-seq and expression values of qRT-PCR for FvMLO genes showed substantial agreement. The FvMLO3 gene, which was grouped in clade III and orthologous to the Arabidopsis,tomato and grapevine genes, was highly expressed in YW compared to other FvMLO genes across varieties. The results showed that FvMLO genes can be used as potential candidates to engineer powdery mildew resistance in strawberry based on MLO suppression or genome editing.

Mapping of powdery mildew resistance genes in melon ( Cucumis melo L.) by bulked segregant analysis

2017 ◽  
Vol 220 ◽  
pp. 160-167 ◽  
Author(s):  
Bing Li ◽  
Yulong Zhao ◽  
Qianglong Zhu ◽  
Zhipeng Zhang ◽  
Chao Fan ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Genes ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Mildew Resistance ◽  
Cucumis Melo L

Genetic linkage map of melon (Cucumis melo L.) and localization of a major QTL for powdery mildew resistance

2010 ◽  
Vol 27 (2) ◽  
pp. 181-192 ◽  
Author(s):  
Fernando J. Yuste-Lisbona ◽  
Carmen Capel ◽  
Emilio Sarria ◽  
Rocío Torreblanca ◽  
María L. Gómez-Guillamón ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Linkage Map ◽  
Cucumis Melo ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L ◽  
Major Qtl

Application of comparative genomics in developing markers tightly linked to the Pm-2F gene for powdery mildew resistance in melon (Cucumis melo L.)

Euphytica ◽  
2012 ◽  
Vol 190 (2) ◽  
pp. 157-168 ◽  
Author(s):  
Chunqiu Zhang ◽  
Yi Ren ◽  
Shaogui Guo ◽  
Haiying Zhang ◽  
Guoyi Gong ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Powdery Mildew ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L

Identification of QTLs for resistance to powdery mildew and SSR markers diagnostic for powdery mildew resistance genes in melon (Cucumis melo L.)

2008 ◽  
Vol 118 (1) ◽  
pp. 165-175 ◽  
Author(s):  
Nobuko Fukino ◽  
Takayoshi Ohara ◽  
Antonio J. Monforte ◽  
Mitsuhiro Sugiyama ◽  
Yoshiteru Sakata ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L

scholarly journals Development of KASP Markers and Identification of a QTL Underlying Powdery Mildew Resistance in Melon (Cucumis melo L.) by Bulked Segregant Analysis and RNA-Seq

2021 ◽  
Vol 11 ◽  
Author(s):  
Yanyan Cao ◽  
Qiannan Diao ◽  
Youyuan Chen ◽  
Haijun Jin ◽  
Yongping Zhang ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Candidate Gene ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Target Area ◽  
Rna Seq ◽  
Mildew Resistance ◽  
Specific Pcr ◽  
Polymorphic Snps ◽  
Kasp Markers

Powdery mildew (PM), caused by Podosphaera xanthii (Px), is one of the most devastating fungal diseases of melon worldwide. The use of resistant cultivars is considered to be the best and most effective approach to control this disease. In this study, an F2 segregating population derived from a cross between a resistant (wm-6) and a susceptible cultivar (12D-1) of melon was used to map major powdery mildew resistance genes using bulked segregant analysis (BSA), in combination with next-generation sequencing (NGS). A novel quantitative trait locus (QTL) named qCmPMR-12 for resistance to PM on chromosome 12 was identified, which ranged from 22.0 Mb to 22.9 Mb. RNA-Seq analysis indicated that the MELO3C002434 gene encoding an ankyrin repeat-containing protein was considered to be the most likely candidate gene that was associated with resistance to PM. Moreover, 15 polymorphic SNPs around the target area were successfully converted to Kompetitive Allele-Specific PCR (KASP) markers (P < 0.0001). The novel QTL and candidate gene identified from this study provide insights into the genetic mechanism of PM resistance in melon, and the tightly linked KASP markers developed in this research can be used for marker-assisted selection (MAS) to improve powdery mildew resistance in melon breeding programs.

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