scholarly journals Facilitating Replication and Reproducibility in Team Science: The ‘projects’ R Package

2019 ◽  
Author(s):  
Nikolas I. Krieger ◽  
Adam T. Perzynski ◽  
Jarrod E. Dalton
Keyword(s):  
R Package ◽  
Study Data ◽  
Reproducible Research ◽  
Data Sets ◽  
Management Tools ◽  
Individual Project ◽  
Shared File ◽  
Database File ◽  
Title Pages ◽  
Free Open Source

AbstractThe contemporary scientific community places a growing emphasis on the reproducibility of research. The projects R package is a free, open-source endeavor created in the interest of facilitating reproducible research workflows. It adds to existing software tools for reproducible research and introduces several practical features that are helpful for scientists and their collaborative research teams. For each individual project, it supplies an intuitive framework for storing raw and cleaned study data sets, and provides script templates for protocol creation, data cleaning, data analysis and manuscript development. Internal databases of project and author information are generated and displayed, and manuscript title pages containing author lists and their affiliations are automatically generated from the internal database. File management tools allow teams to organize multiple projects. When used on a shared file system, multiple researchers can harmoniously contribute to the same project in a less punctuated manner, reducing the frequency of misunderstandings and the need for status updates.

scholarly journals MUREN: a robust and multi-reference approach of RNA-seq transcript normalization

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yance Feng ◽  
Lei M. Li
Keyword(s):  
Biological Significance ◽  
Housekeeping Genes ◽  
R Package ◽  
Data Sets ◽  
Statistical Regression ◽  
Rna Seq ◽  
Least Trimmed Squares ◽  
Standard Data ◽  
Wide Range ◽  
Multiple References

Abstract Background Normalization of RNA-seq data aims at identifying biological expression differentiation between samples by removing the effects of unwanted confounding factors. Explicitly or implicitly, the justification of normalization requires a set of housekeeping genes. However, the existence of housekeeping genes common for a very large collection of samples, especially under a wide range of conditions, is questionable. Results We propose to carry out pairwise normalization with respect to multiple references, selected from representative samples. Then the pairwise intermediates are integrated based on a linear model that adjusts the reference effects. Motivated by the notion of housekeeping genes and their statistical counterparts, we adopt the robust least trimmed squares regression in pairwise normalization. The proposed method (MUREN) is compared with other existing tools on some standard data sets. The goodness of normalization emphasizes on preserving possible asymmetric differentiation, whose biological significance is exemplified by a single cell data of cell cycle. MUREN is implemented as an R package. The code under license GPL-3 is available on the github platform: github.com/hippo-yf/MUREN and on the conda platform: anaconda.org/hippo-yf/r-muren. Conclusions MUREN performs the RNA-seq normalization using a two-step statistical regression induced from a general principle. We propose that the densities of pairwise differentiations are used to evaluate the goodness of normalization. MUREN adjusts the mode of differentiation toward zero while preserving the skewness due to biological asymmetric differentiation. Moreover, by robustly integrating pre-normalized counts with respect to multiple references, MUREN is immune to individual outlier samples.

scholarly journals COVID-19 in patients undergoing chronic kidney replacement therapy and kidney transplant recipients in Scotland: findings and experience from the Scottish renal registry

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Samira Bell ◽  
◽  
Jacqueline Campbell ◽  
Jackie McDonald ◽  
Martin O’Neill ◽  
...  
Keyword(s):  
Public Health ◽  
Replacement Therapy ◽  
Study Data ◽  
Data Sets ◽  
Kidney Transplant Recipients ◽  
The Public ◽  
Transplant Patients ◽  
Kidney Replacement Therapy ◽  
Observational Cohort ◽  
Respiratory Coronavirus

Abstract Background Infection with the severe acute respiratory coronavirus 2 (SARS-CoV-2) has led to a worldwide pandemic with coronavirus disease 2019 (COVID-19), the disease caused by SARS-CoV-2, overwhelming healthcare systems globally. Preliminary reports suggest a high incidence of infection and mortality with SARS-CoV-2 in patients receiving kidney replacement therapy (KRT). The aims of this study are to report characteristics, rates and outcomes of all patients affected by infection with SARS-CoV-2 undergoing KRT in Scotland. Methods Study design was an observational cohort study. Data were linked between the Scottish Renal Registry, Health Protection Scotland and the Scottish Intensive Care Society Audit Group national data sets using a unique patient identifier (Community Health Index (CHI)) for each individual by the Public Health and Intelligence unit of Public Health, Scotland. Descriptive statistics and survival analyses were performed. Results During the period 1st March 2020 to 31st May 2020, 110 patients receiving KRT tested positive for SARS-CoV-2 amounting to 2% of the prevalent KRT population. Of those affected, 86 were receiving haemodialysis or peritoneal dialysis and 24 had a renal transplant. Patients who tested positive were older and more likely to reside in more deprived postcodes. Mortality was high at 26.7% in the dialysis patients and 29.2% in the transplant patients. Conclusion The rate of detected SARS-CoV-2 in people receiving KRT in Scotland was relatively low but with a high mortality for those demonstrating infection. Although impossible to confirm, it appears that the measures taken within dialysis units coupled with the national shielding policy, have been effective in protecting this population from infection.

scholarly journals Gene Ontology Analysis of GWA Study Data Sets Provides Insights into the Biology of Bipolar Disorder

2009 ◽  
Vol 85 (1) ◽  
pp. 13-24 ◽  
Author(s):  
Peter Holmans ◽  
Elaine K. Green ◽  
Jaspreet Singh Pahwa ◽  
Manuel A.R. Ferreira ◽  
Shaun M. Purcell ◽  
...  
Keyword(s):  
Bipolar Disorder ◽  
Gene Ontology ◽  
Study Data ◽  
Data Sets ◽  
Gene Ontology Analysis

The New Statistics with R

2021 ◽  
Author(s):  
Andy Hector
Keyword(s):  
Linear Model ◽  
Evolutionary Biology ◽  
Environmental Science ◽  
Research Training ◽  
Model Analysis ◽  
Scientific Data ◽  
Information Criteria ◽  
Reproducible Research ◽  
Data Sets ◽  
R Programming

Statistics is a fundamental component of the scientific toolbox, but learning the basics of this area of mathematics is one of the most challenging parts of a research training. This book gives an up-to-date introduction to the classical techniques and modern extensions of linear-model analysis—one of the most useful approaches in the analysis of scientific data in the life and environmental sciences. The book emphasizes an estimation-based approach that takes account of recent criticisms of overuse of probability values and introduces the alternative approach using information criteria. The book is based on the use of the open-source R programming language for statistics and graphics, which is rapidly becoming the lingua franca in many areas of science. This second edition adds new chapters, including one discussing some of the complexities of linear-model analysis and another introducing reproducible research documents using the R Markdown package. Statistics is introduced through worked analyses performed in R using interesting data sets from ecology, evolutionary biology, and environmental science. The data sets and R scripts are available as supporting material.

scholarly journals FORESEE: a tool for the systematic comparison of translational drug response modeling pipelines

2018 ◽  
Author(s):  
Lisa-Katrin Turnhoff ◽  
Ali Hadizadeh Esfahani ◽  
Maryam Montazeri ◽  
Nina Kusch ◽  
Andreas Schuppert
Keyword(s):  
Drug Response ◽  
Drug Efficacy ◽  
Response Prediction ◽  
R Package ◽  
Supplementary Information ◽  
Supplementary File ◽  
Data Sets ◽  
Training Algorithms ◽  
Model Training

Translational models that utilize omics data generated in in vitro studies to predict the drug efficacy of anti-cancer compounds in patients are highly distinct, which complicates the benchmarking process for new computational approaches. In reaction to this, we introduce the uniFied translatiOnal dRug rESponsE prEdiction platform FORESEE, an open-source R-package. FORESEE not only provides a uniform data format for public cell line and patient data sets, but also establishes a standardized environment for drug response prediction pipelines, incorporating various state-of-the-art preprocessing methods, model training algorithms and validation techniques. The modular implementation of individual elements of the pipeline facilitates a straightforward development of combinatorial models, which can be used to re-evaluate and improve already existing pipelines as well as to develop new ones. Availability and Implementation: FORESEE is licensed under GNU General Public License v3.0 and available at https://github.com/JRC-COMBINE/FORESEE . Supplementary Information: Supplementary Files 1 and 2 provide detailed descriptions of the pipeline and the data preparation process, while Supplementary File 3 presents basic use cases of the package. Contact: [email protected]

scholarly journals SpiderSeqR: an R package for crawling the web of high-throughput multi-omic data repositories for data-sets and annotation

2020 ◽  
Author(s):  
Anna M. Sozanska ◽  
Charles Fletcher ◽  
Dóra Bihary ◽  
Shamith A. Samarajiwa
Keyword(s):  
High Throughput ◽  
R Package ◽  
Data Reuse ◽  
Massively Parallel ◽  
Data Sets ◽  
Similar Data ◽  
Data Generation ◽  
Data Repositories ◽  
Public Data ◽  
Omic Data

AbstractMore than three decades ago, the microarray revolution brought about high-throughput data generation capability to biology and medicine. Subsequently, the emergence of massively parallel sequencing technologies led to many big-data initiatives such as the human genome project and the encyclopedia of DNA elements (ENCODE) project. These, in combination with cheaper, faster massively parallel DNA sequencing capabilities, have democratised multi-omic (genomic, transcriptomic, translatomic and epigenomic) data generation leading to a data deluge in bio-medicine. While some of these data-sets are trapped in inaccessible silos, the vast majority of these data-sets are stored in public data resources and controlled access data repositories, enabling their wider use (or misuse). Currently, most peer reviewed publications require the deposition of the data-set associated with a study under consideration in one of these public data repositories. However, clunky and difficult to use interfaces, subpar or incomplete annotation prevent discovering, searching and filtering of these multi-omic data and hinder their re-purposing in other use cases. In addition, the proliferation of multitude of different data repositories, with partially redundant storage of similar data are yet another obstacle to their continued usefulness. Similarly, interfaces where annotation is spread across multiple web pages, use of accession identifiers with ambiguous and multiple interpretations and lack of good curation make these data-sets difficult to use. We have produced SpiderSeqR, an R package, whose main features include the integration between NCBI GEO and SRA databases, enabling an integrated unified search of SRA and GEO data-sets and associated annotations, conversion between database accessions, as well as convenient filtering of results and saving past queries for future use. All of the above features aim to promote data reuse to facilitate making new discoveries and maximising the potential of existing data-sets.Availabilityhttps://github.com/ss-lab-cancerunit/SpiderSeqR

scholarly journals The R package “eseis” – a software toolbox for environmental seismology

2018 ◽  
Vol 6 (3) ◽  
pp. 669-686 ◽  
Author(s):  
Michael Dietze
Keyword(s):  
Data Science ◽  
Earth Science ◽  
Science Research ◽  
Worked Examples ◽  
R Package ◽  
Research Field ◽  
Data Sets ◽  
Research Fields ◽  
Scientific Disciplines ◽  
Analysis Environment

Abstract. Environmental seismology is the study of the seismic signals emitted by Earth surface processes. This emerging research field is at the intersection of seismology, geomorphology, hydrology, meteorology, and further Earth science disciplines. It amalgamates a wide variety of methods from across these disciplines and ultimately fuses them in a common analysis environment. This overarching scope of environmental seismology requires a coherent yet integrative software which is accepted by many of the involved scientific disciplines. The statistic software R has gained paramount importance in the majority of data science research fields. R has well-justified advances over other mostly commercial software, which makes it the ideal language to base a comprehensive analysis toolbox on. The article introduces the avenues and needs of environmental seismology, and how these are met by the R package eseis. The conceptual structure, example data sets, and available functions are demonstrated. Worked examples illustrate possible applications of the package and in-depth descriptions of the flexible use of the functions. The package has a registered DOI, is available under the GPL licence on the Comprehensive R Archive Network (CRAN), and is maintained on GitHub.

scholarly journals Decomposing the Apoptosis Pathway Into Biologically Interpretable Principal Components

2018 ◽  
Vol 17 ◽  
pp. 117693511877108 ◽  
Author(s):  
Min Wang ◽  
Steven M Kornblau ◽  
Kevin R Coombes
Keyword(s):  
Principal Components ◽  
Myeloid Leukemia ◽  
Principal Component ◽  
R Package ◽  
Biological Data ◽  
Data Sets ◽  
Proteomics Data ◽  
Data Set ◽  
Apoptosis Pathway ◽  
Biological Interpretation

Principal component analysis (PCA) is one of the most common techniques in the analysis of biological data sets, but applying PCA raises 2 challenges. First, one must determine the number of significant principal components (PCs). Second, because each PC is a linear combination of genes, it rarely has a biological interpretation. Existing methods to determine the number of PCs are either subjective or computationally extensive. We review several methods and describe a new R package, PCDimension, that implements additional methods, the most important being an algorithm that extends and automates a graphical Bayesian method. Using simulations, we compared the methods. Our newly automated procedure is competitive with the best methods when considering both accuracy and speed and is the most accurate when the number of objects is small compared with the number of attributes. We applied the method to a proteomics data set from patients with acute myeloid leukemia. Proteins in the apoptosis pathway could be explained using 6 PCs. By clustering the proteins in PC space, we were able to replace the PCs by 6 “biological components,” 3 of which could be immediately interpreted from the current literature. We expect this approach combining PCA with clustering to be widely applicable.

scholarly journals How to Automatically Document Data With the codebook Package to Facilitate Data Reuse

2019 ◽  
Vol 2 (2) ◽  
pp. 169-187 ◽  
Author(s):  
Ruben C. Arslan
Keyword(s):  
R Package ◽  
Data Reuse ◽  
Data Sets ◽  
Data Set ◽  
Psychological Scales ◽  
Rich Data ◽  
Data Documentation ◽  
Machine Readable ◽  
Basic Standards ◽  
Existing Data

Data documentation in psychology lags behind not only many other disciplines, but also basic standards of usefulness. Psychological scientists often prefer to invest the time and effort that would be necessary to document existing data well in other duties, such as writing and collecting more data. Codebooks therefore tend to be unstandardized and stored in proprietary formats, and they are rarely properly indexed in search engines. This means that rich data sets are sometimes used only once—by their creators—and left to disappear into oblivion. Even if they can find an existing data set, researchers are unlikely to publish analyses based on it if they cannot be confident that they understand it well enough. My codebook package makes it easier to generate rich metadata in human- and machine-readable codebooks. It uses metadata from existing sources and automates some tedious tasks, such as documenting psychological scales and reliabilities, summarizing descriptive statistics, and identifying patterns of missingness. The codebook R package and Web app make it possible to generate a rich codebook in a few minutes and just three clicks. Over time, its use could lead to psychological data becoming findable, accessible, interoperable, and reusable, thereby reducing research waste and benefiting both its users and the scientific community as a whole.

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