Optimal sample size calculation for null hypothesis significance tests

Traditional study design tools for estimating appropriate sample sizes are not consistently used in ecology and can lead to low statistical power to detect biologically relevant effects. We have developed a new approach to estimating optimal sample sizes, requiring only three parameters; a maximum acceptable average of α and β, a critical effect size of minimum biological relevance, and an estimate of the relative costs of Type I vs. Type II errors.This approach can be used to show the general circumstances under which different combinations of critical effect sizes and maximum acceptable combinations of α and β are attainable for different statistical tests. The optimal α sample size estimation approach can require fewer samples than traditional sample size estimation methods when costs of Type I and II errors are assumed to be equal but recommends comparatively more samples for increasingly unequal Type I vs. Type II errors costs. When sampling costs and absolute costs of Type I and II errors are known, optimal sample size estimation can be used to determine the smallest sample size at which the cost of an additional sample outweighs its associated reduction in errors. Optimal sample size estimation constitutes a more flexible and intuitive tool than traditional sample size estimation approaches, given the constraints and unknowns commonly faced by ecologists during study.