scholarly journals GWAS hints at pleiotropic roles for FLOWERING LOCUS T in flowering time and yield-related traits in canola

2019 ◽  
Author(s):  
Harsh Raman ◽  
Rosy Raman ◽  
Yu Qiu ◽  
Avilash Singh Yadav ◽  
Sridevi Sureshkumar ◽  
...  
Keyword(s):  
Grain Yield ◽  
Flowering Time ◽  
Shoot Biomass ◽  
Oilseed Crop ◽  
Flowering Locus T ◽  
Dh Population ◽  
Plant Emergence ◽  
Flowering Locus ◽  
The Right ◽  
Genomic Regions

AbstractTransition to flowering at the right time is critical for local adaptation and to maximize seed yield in canola, which is an important oilseed crop. There is extensive variation among canola varieties in flowering time. However, our understanding of underlying genes and their role in canola productivity is still limited. We reveal natural variation in flowering time and response to photoperiod in a diverse GWAS panel (up to 368 accessions) of canola and identify associated SNPs across multiple experiments. Complementary QTL and eQTL mapping studies were also conducted in an Australian doubled haploid (DH) population for flowering time and other grain yield related traits. We show that several associations that were repeatedly detected across experiments map in the vicinity of FLOWERING LOCUS T (FT) paralogues and its known transcriptional regulators. QTL mapping study in a DH population detected consistent genomic regions close to FT paralogs. FT sequences vary between accessions and FT expression in field and controlled environment grown plants was correlated with flowering time. FT paralogs displayed association not only with flowering time, but also with plant emergence, shoot biomass and grain yield. Our findings suggest that FT paralogs not only modulate flowering time but also modulate yield-related productivity traits in canola.HighlightThe genetic association, eQTL and expression analyses suggest that FT paralogs have multifaceted roles in canola flowering time, plant development and productivity traits.

scholarly journals A novel deletion in FLOWERING LOCUS T modulates flowering time in winter oilseed rape

2021 ◽  
Author(s):  
Paul Vollrath ◽  
Harmeet S. Chawla ◽  
Sarah V. Schiessl ◽  
Iulian Gabur ◽  
HueyTyng Lee ◽  
...  
Keyword(s):  
Association Mapping ◽  
Flowering Time ◽  
Oilseed Rape ◽  
Major Effect ◽  
Structural Variants ◽  
Winter Oilseed Rape ◽  
Flowering Locus T ◽  
Winter Type ◽  
Long Read ◽  
Flowering Locus

Abstract Key message A novel structural variant was discovered in the FLOWERING LOCUS T orthologue BnaFT.A02 by long-read sequencing. Nested association mapping in an elite winter oilseed rape population revealed that this 288 bp deletion associates with early flowering, putatively by modification of binding-sites for important flowering regulation genes. Abstract Perfect timing of flowering is crucial for optimal pollination and high seed yield. Extensive previous studies of flowering behavior in Brassica napus (canola, rapeseed) identified mutations in key flowering regulators which differentiate winter, semi-winter and spring ecotypes. However, because these are generally fixed in locally adapted genotypes, they have only limited relevance for fine adjustment of flowering time in elite cultivar gene pools. In crosses between ecotypes, the ecotype-specific major-effect mutations mask minor-effect loci of interest for breeding. Here, we investigated flowering time in a multiparental mapping population derived from seven elite winter oilseed rape cultivars which are fixed for major-effect mutations separating winter-type rapeseed from other ecotypes. Association mapping revealed eight genomic regions on chromosomes A02, C02 and C03 associating with fine modulation of flowering time. Long-read genomic resequencing of the seven parental lines identified seven structural variants coinciding with candidate genes for flowering time within chromosome regions associated with flowering time. Segregation patterns for these variants in the elite multiparental population and a diversity set of winter types using locus-specific assays revealed significant associations with flowering time for three deletions on chromosome A02. One of these was a previously undescribed 288 bp deletion within the second intron of FLOWERING LOCUS T on chromosome A02, emphasizing the advantage of long-read sequencing for detection of structural variants in this size range. Detailed analysis revealed the impact of this specific deletion on flowering-time modulation under extreme environments and varying day lengths in elite, winter-type oilseed rape.

scholarly journals Isolation and Functional Characterization of MsFTa, a FLOWERING LOCUS T Homolog from Alfalfa (Medicago sativa)

2019 ◽  
Vol 20 (8) ◽  
pp. 1968 ◽  
Author(s):  
Junmei Kang ◽  
Tiejun Zhang ◽  
Tao Guo ◽  
Wang Ding ◽  
Ruicai Long ◽  
...  
Keyword(s):  
Flowering Time ◽  
Functional Characterization ◽  
Flowering Locus T ◽  
Over Expression ◽  
Long Day ◽  
Gfp Fusion Protein ◽  
Intron Structure ◽  
Flowering Locus ◽  
Flowering Regulation

The production of hay and seeds of alfalfa, an important legume forage for the diary industry worldwide, is highly related to flowering time, which has been widely reported to be integrated by FLOWERING LOCUS T (FT). However, the function of FT(s) in alfalfa is largely unknown. Here, we identified MsFTa, an FT ortholog in alfalfa, and characterized its role in flowering regulation. MsFTa shares the conserved exon/intron structure of FTs, and MsFTa is 98% identical to MtFTa1 in Medicago trucatula. MsFTa was diurnally regulated with a peak before the dark period, and was preferentially expressed in leaves and floral buds. Transient expression of MsFTa-GFP fusion protein demonstrated its localization in the nucleus and cytoplasm. When ectopically expressed, MsFTa rescued the late-flowering phenotype of ft mutants from Arabidopsis and M. trucatula. MsFTa over-expression plants of both Arabidopsis and M. truncatula flowered significantly earlier than the non-transgenic controls under long day conditions, indicating that exogenous MsFTa strongly accelerated flowering. Hence, MsFTa functions positively in flowering promotion, suggesting that MsFTa may encode a florigen that acts as a key regulator in the flowering pathway. This study provides an effective candidate gene for optimizing alfalfa flowering time by genetically manipulating the expression of MsFTa.

scholarly journals Dehydroabietinal promotes flowering time and plant defense in Arabidopsis via the autonomous pathway genes FLOWERING LOCUS D, FVE, and RELATIVE OF EARLY FLOWERING 6

2020 ◽  
Vol 71 (16) ◽  
pp. 4903-4913 ◽  
Author(s):  
Zulkarnain Chowdhury ◽  
Devasantosh Mohanty ◽  
Mrunmay K Giri ◽  
Barney J Venables ◽  
Ratnesh Chaturvedi ◽  
...  
Keyword(s):  
Flowering Time ◽  
Systemic Acquired Resistance ◽  
Defense Mechanism ◽  
Acquired Resistance ◽  
Early Flowering ◽  
Negative Regulator ◽  
Flowering Locus T ◽  
Autonomous Pathway ◽  
Abietane Diterpenoids ◽  
Flowering Locus

Abstract Abietane diterpenoids are tricyclic diterpenes whose biological functions in angiosperms are largely unknown. Here, we show that dehydroabietinal (DA) fosters transition from the vegetative phase to reproductive development in Arabidopsis thaliana by promoting flowering time. DA’s promotion of flowering time was mediated through up-regulation of the autonomous pathway genes FLOWERING LOCUS D (FLD), RELATIVE OF EARLY FLOWERING 6 (REF6), and FVE, which repress expression of FLOWERING LOCUS C (FLC), a negative regulator of the key floral integrator FLOWERING LOCUS T (FT). Our results further indicate that FLD, REF6, and FVE are also required for systemic acquired resistance (SAR), an inducible defense mechanism that is also activated by DA. However, unlike flowering time, FT was not required for DA-induced SAR. Conversely, salicylic acid, which is essential for the manifestation of SAR, was not required for the DA-promoted flowering time. Thus, although the autonomous pathway genes FLD, REF6, and FVE are involved in SAR and flowering time, these biological processes are not interdependent. We suggest that SAR and flowering time signaling pathways bifurcate at a step downstream of FLD, REF6, and FVE, with an FLC-dependent arm controlling flowering time, and an FLC-independent pathway controlling SAR.

scholarly journals GWAS hints at pleiotropic roles for FLOWERING LOCUS T in flowering time and yield-related traits in canola

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Harsh Raman ◽  
Rosy Raman ◽  
Yu Qiu ◽  
Avilash Singh Yadav ◽  
Sridevi Sureshkumar ◽  
...  
Keyword(s):  
Flowering Time ◽  
Flowering Locus T ◽  
Flowering Locus

scholarly journals Natural Variation of the RICE FLOWERING LOCUS T 1 Contributes to Flowering Time Divergence in Rice

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e75959 ◽  
Author(s):  
Eri Ogiso-Tanaka ◽  
Kazuki Matsubara ◽  
Shin-ichi Yamamoto ◽  
Yasunori Nonoue ◽  
Jianzhong Wu ◽  
...  
Keyword(s):  
Flowering Time ◽  
Natural Variation ◽  
Flowering Locus T ◽  
Flowering Locus

scholarly journals BBX19 Interacts with CONSTANS to Repress FLOWERING LOCUS T Transcription, Defining a Flowering Time Checkpoint in Arabidopsis

2014 ◽  
Vol 26 (9) ◽  
pp. 3589-3602 ◽  
Author(s):  
C.-Q. Wang ◽  
C. Guthrie ◽  
M. K. Sarmast ◽  
K. Dehesh
Keyword(s):  
Flowering Time ◽  
Flowering Locus T ◽  
Flowering Locus

Emergence, height, grain yield and oil content of camelina and canola grown in saline media

2010 ◽  
Vol 90 (1) ◽  
pp. 151-164 ◽  
Author(s):  
H. Steppuhn ◽  
K C Falk ◽  
R. Zhou
Keyword(s):  
Grain Yield ◽  
Salinity Tolerance ◽  
Oil Content ◽  
Salt Resistance ◽  
Shoot Biomass ◽  
Tolerance Index ◽  
False Flax ◽  
Testing Facility ◽  
Plant Emergence ◽  
Tolerance Testing

Crops of CS15 camelina and InVigor 9590 canola, grown under field conditions in Canada’s Salinity Tolerance Testing Facility, were evaluated for plant emergence, height, shoot biomass, grain yield, oil content and composition. The crops were seeded directly into sand tanks flushed four times daily with hydroponics consisting of nutrients and salts ranging in salinity from negligible to severe. Sulphate-based solutions averaging 1.4 (nutrients only), 3.0, 6.0, 10.0, 14.7, 19.9, and 27.0 dS m-1 in electrical conductivity (ECsol) resulted in respective cumulative emergence of 99.0 to 42.1% for the camelina and 99.3 to 79.6% for the canola and showed statistical differences only at the two highest salinity treatments. Plant height differences between the camelina and the canola increased as salinity increased: from 16% at 1.4 dS m-1 to 60% at 19.9 dS m-1. Grain yields under salinity relative to the salt-free yield decreased more for the camelina than for the canola at all ECsol-levels. The salinity tolerance index based on the ECsol -value at 50% of the maximum grain yield indicated that the camelina registered less than half (7.4) of that for the canola (18.0). The percentage oil content of the canola oilseed averaged 40% until salinity exceeded 20 dS m-1, while that of the camelina averaged 35% until 10 dS m-1, before declining. Key words: Camelina sativa, false flax, salinity tolerance, salt resistance, Brassica napus, canola

scholarly journals Evolution of the miR5200-FLOWERING LOCUS T flowering time regulon in the temperate grass subfamily Pooideae

2017 ◽  
Vol 114 ◽  
pp. 111-121 ◽  
Author(s):  
Meghan McKeown ◽  
Marian Schubert ◽  
Jill C. Preston ◽  
Siri Fjellheim
Keyword(s):  
Flowering Time ◽  
Flowering Locus T ◽  
Flowering Locus

Clock-Controlled and FLOWERING LOCUS T (FT)-Dependent Photoperiodic Pathway inLotus japonicusII: Characterization of a MicroRNA Implicated in the Control of Flowering Time

2013 ◽  
Vol 77 (6) ◽  
pp. 1179-1185 ◽  
Author(s):  
Takafumi YAMASHINO ◽  
Saori YAMAWAKI ◽  
Emi HAGUI ◽  
Kai ISHIDA ◽  
Hanayo UEOKA-NAKANISHI ◽  
...  
Keyword(s):  
Flowering Time ◽  
Flowering Locus T ◽  
Flowering Locus
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