scholarly journals Design of a comprehensive microfluidic and microscopic toolbox for the ultra-wide spatio-temporal study of plant protoplasts development and physiology

2019 ◽  
Author(s):  
K. Sakai ◽  
F. Charlot ◽  
T. Le Saux ◽  
S. Bonhomme ◽  
F. Nogué ◽  
...  
Keyword(s):  
Cell Wall ◽  
Physcomitrella Patens ◽  
Low Cost ◽  
Plant Cells ◽  
Single Layer ◽  
Experimental Setup ◽  
Plant Protoplasts ◽  
Temporal Study ◽  
Custom Made ◽  
Spatio Temporal

ABSTRACTBackgroundOne of the main features of plant cells is their strong plasticity, and their propensity to regenerate an organism from a single cell. Plant protoplasts are basic plant cells units in which the pecto-cellulosic cell wall has been removed, but the plasma membrane is intact. One of the main features of plant cells is their strong plasticity, which in some species, can be very close from what is defined as cell totipotency. Methods and differentiation protocols used in plant physiology and plant biology usually involve macroscopic vessels and containers that make difficult, for example, to follow the fate of the same protoplast all along its full development cycle, but also to perform continuous studies of the influence of various gradients in this context. These limits have hampered the precise study of regeneration processes.ResultsHerein, we present the design of a comprehensive, physiologically relevant, easy-to-use and low-cost microfluidic and microscopic setup for the monitoring of Physcomitrella patens (P. patens) growth and development on a long-term basis. The experimental solution we developed is made of two parts (i) a microfluidic chip composed of a single layer of about a hundred flow-through microfluidic traps for the immobilization of protoplasts, and (ii) a low-cost, light-controlled, custom-made microscope allowing the continuous recording of the moss development in physiological conditions.We validated the experimental setup with three proofs of concepts: (i) the kinetic monitoring of first division steps and cell wall regeneration, (ii) the influence of the photoperiod on growth of the protonemata, and (iii) finally the induction of leafy buds using a phytohormone, cytokinin.ConclusionsWe developed the design of a comprehensive, physiologically relevant, easy-to-use and low-cost experimental setup for the study of P. patens development in a microfluidic environment. This setup allows imaging of P. patens development at high resolution and over long time periods.

scholarly journals Design of a comprehensive microfluidic and microscopic toolbox for the ultra-wide spatio-temporal study of plant protoplasts development and physiology

Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Kaori Sakai ◽  
Florence Charlot ◽  
Thomas Le Saux ◽  
Sandrine Bonhomme ◽  
Fabien Nogué ◽  
...  
Keyword(s):  
Plant Protoplasts ◽  
Temporal Study ◽  
Spatio Temporal

Crystalline order to a resolution of 4.7 A in the sheath of Methanospirilium hungatii: A cross-beta structure

1984 ◽  
Vol 42 ◽  
pp. 214-215
Author(s):  
Murray Stewart ◽  
T.J. Beveridge ◽  
D. Sprott
Keyword(s):  
Cell Wall ◽  
Single Layer ◽  
Uranyl Acetate ◽  
Optical Diffraction ◽  
Tube Axis ◽  
Basic Subunit ◽  
Beta Structure ◽  
Diffraction Patterns ◽  
Protein Treatment ◽  
General Appearance

The archaebacterium Methanospirillum hungatii has a sheath as part of its cell wall which is composed mainly of protein. Treatment with dithiothreitol or NaOH released the intact sheaths and electron micrographs of this material negatively stained with uranyl acetate showed flattened hollow tubes, about 0.5 μm diameter and several microns long, in which the patterns from the top and bottom were superimposed. Single layers, derived from broken tubes, were also seen and were more simply analysed. Figure 1 shows the general appearance of a single layer. There was a faint axial periodicity at 28.5 A, which was stronger at irregular multiples of 28.5 A (3 and 4 times were most common), and fine striations were also seen at about 3° to the tube axis. Low angle electron diffraction patterns (not shown) and optical diffraction patterns (Fig. 2) from these layers showed a complex meridian (as a result of the irregular nature of the repeat along the tube axis) which showed a clear maximum at 28.5 A, consistent with the basic subunit spacing.

Enrichment of vitronectin- and fibronectin-like proteins in NaCI-adapted plant cells and evidence for their involvement in plasma membrane-cell wall adhesion

1993 ◽  
Vol 3 (5) ◽  
pp. 637-646 ◽  
Author(s):  
Jian-Kang Zhu ◽  
Jun Shi ◽  
Utpal Singh ◽  
Sarah E. Wyatt ◽  
Ray A. Bressan ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Plant Cells ◽  
Membrane Cell

Bamboo Waste-based Bio-composite Substance: An application for Low-cost Construction Materials

2020 ◽  
Vol 4 (1) ◽  
pp. 41-48
Author(s):  
Teodoro Astorga Amatosa ◽  
Michael E. Loretero
Keyword(s):  
Composite Materials ◽  
Sea Water ◽  
Raw Materials ◽  
Low Cost ◽  
Construction Materials ◽  
Single Layer ◽  
High Strength ◽  
Green Technology ◽  
Experimental Medium ◽  
Natural Treatment

Bamboo is a lightweight and high-strength raw materials that encouraged researchers to investigate and explore, especially in the field of biocomposite and declared as one of the green-technology on the environment as fully accountable as eco-products. This research was to assess the technical feasibility of making single-layer experimental Medium-Density Particleboard panels from the bamboo waste of a three-year-old (Dendrocalamus asper). Waste materials were performed to produce composite materials using epoxy resin (C21H25C105) from a natural treatment by soaking with an average of pH 7.6 level of sea-water. Three different types of MDP produced, i.e., bamboo waste strip MDP (SMDP), bamboo waste chips MDP (CMDP) and bamboo waste mixed strip-chips MDP (MMDP) by following the same process. The experimental panels tested for their physical-mechanical properties according to the procedures defined by ASTM D1037-12. Conclusively, even the present study shows properties of MDP with higher and comparable to other composite materials; further research must be given better attention as potential substitute to be used as hardwood materials, especially in the production, design, and construction usage.

A Novel Wideband transition from Substrate Integrated Waveguide to Rectangular Waveguide

2020 ◽  
Vol 10 ◽  
Author(s):  
Keyur Mahant ◽  
Hiren Mewada ◽  
Amit Patel ◽  
Alpesh Vala ◽  
Jitendra Chaudhari
Keyword(s):  
Rectangular Waveguide ◽  
Printed Circuit Board ◽  
Low Cost ◽  
Single Layer ◽  
Circuit Board ◽  
Substrate Integrated Waveguide ◽  
Ka Band ◽  
Better Than ◽  
Millimeter Wave Circuits ◽  
Wideband Transition

Aim: In this article, wideband substrate integrated waveguide (SIW) and rectangular waveguide (RWG) transition operating in Ka-band is proposed Objective: In this article, wideband substrate integrated waveguide (SIW) and rectangular waveguide (RWG) transition operating in Ka-band is proposed. Method: Coupling patch etched on the SIW cavity to couple the electromagnetic energy from SIW to RWG. Moreover, metasurface is introduced into the radiating patch to enhance bandwidth. To verify the functionality of the proposed structure back to back transition is designed and fabricated on a single layer substrate using standard printed circuit board (PCB) fabrication technology. Results: Measured results matches with the simulation results, measured insertion loss is less than 1.2 dB and return loss is better than 3 dB for the frequency range of 28.8 to 36.3 GHz. By fabricating transition with 35 SRRs bandwidth of the proposed transition can be improved. Conclusion: The proposed transition has advantages like compact in size, easy to fabricate, low cost and wide bandwidth. Proposed structure is a good candidate for millimeter wave circuits and systems.

scholarly journals Short-Time Recurrences of Plasmodium vivax Malaria as a Public Health Proxy for Chloroquine-Resistance Surveillance: A Spatio-Temporal Study in the Brazilian Amazon

2021 ◽  
Vol 18 (10) ◽  
pp. 5061
Author(s):  
Antonio A. S. Balieiro ◽  
Andre M. Siqueira ◽  
Gisely C. Melo ◽  
Wuelton M. Monteiro ◽  
Vanderson S. Sampaio ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Multilevel Models ◽  
Malaria Incidence ◽  
Chloroquine Resistance ◽  
Spatial Clusters ◽  
Temporal Study ◽  
Plasmodium Vivax Malaria ◽  
Spatio Temporal ◽  
Spatially Varying ◽  
Short Time

In Brazil, malaria caused by Plasmodium vivax presents control challenges due to several reasons, among them the increasing possibility of failure of P. vivax treatment due to chloroquine-resistance (CQR). Despite limited reports of CQR, more extensive studies on the actual magnitude of resistance are still needed. Short-time recurrences of malaria cases were analyzed in different transmission scenarios over three years (2005, 2010, and 2015), selected according to malaria incidence. Multilevel models (binomial) were used to evaluate association of short-time recurrences with variables such as age. The zero-inflated Poisson scan model (scanZIP) was used to detect spatial clusters of recurrences up to 28 days. Recurrences compose less than 5% of overall infection, being more frequent in the age group under four years. Recurrences slightly increased incidence. No fixed clusters were detected throughout the period, although there are clustering sites, spatially varying over the years. This is the most extensive analysis of short-time recurrences worldwide which addresses the occurrence of P. vivax CQR. As an important step forward in malaria elimination, policymakers should focus their efforts on young children, with an eventual shift in the first line of malaria treatment to P. vivax.

scholarly journals Biomechanics of Trail Running Performance: Quantification of Spatio-Temporal Parameters by Using Low Cost Sensors in Ecological Conditions

2021 ◽  
Vol 11 (5) ◽  
pp. 2093
Author(s):  
Noé Perrotin ◽  
Nicolas Gardan ◽  
Arnaud Lesprillier ◽  
Clément Le Goff ◽  
Jean-Marc Seigneur ◽  
...  
Keyword(s):  
Heart Rate ◽  
Low Cost ◽  
Step Length ◽  
Measurement Unit ◽  
Ecological Conditions ◽  
Sports Science ◽  
Ground Contact Time ◽  
Spatio Temporal ◽  
Performance Results ◽  
Trail Running

The recent popularity of trail running and the use of portable sensors capable of measuring many performance results have led to the growth of new fields in sports science experimentation. Trail running is a challenging sport; it usually involves running uphill, which is physically demanding and therefore requires adaptation to the running style. The main objectives of this study were initially to use three “low-cost” sensors. These low-cost sensors can be acquired by most sports practitioners or trainers. In the second step, measurements were taken in ecological conditions orderly to expose the runners to a real trail course. Furthermore, to combine the collected data to analyze the most efficient running techniques according to the typology of the terrain were taken, as well on the whole trail circuit of less than 10km. The three sensors used were (i) a Stryd sensor (Stryd Inc. Boulder CO, USA) based on an inertial measurement unit (IMU), 6 axes (3-axis gyroscope, 3-axis accelerometer) fixed on the top of the runner’s shoe, (ii) a Global Positioning System (GPS) watch and (iii) a heart belt. Twenty-eight trail runners (25 men, 3 women: average age 36 ± 8 years; height: 175.4 ± 7.2 cm; weight: 68.7 ± 8.7 kg) of different levels completed in a single race over a 8.5 km course with 490 m of positive elevation gain. This was performed with different types of terrain uphill (UH), downhill (DH), and road sections (R) at their competitive race pace. On these sections of the course, cadence (SF), step length (SL), ground contact time (GCT), flight time (FT), vertical oscillation (VO), leg stiffness (Kleg), and power (P) were measured with the Stryd. Heart rate, speed, ascent, and descent speed were measured by the heart rate belt and the GPS watch. This study showed that on a ≤10 km trail course the criteria for obtaining a better time on the loop, determined in the test, was consistency in the effort. In a high percentage of climbs (>30%), two running techniques stand out: (i) maintaining a high SF and a short SL and (ii) decreasing the SF but increasing the SL. In addition, it has been shown that in steep (>28%) and technical descents, the average SF of the runners was higher. This happened when their SL was shorter in lower steep and technically challenging descents.

scholarly journals A single layer deposition of Li-doped mesoporous TiO2 beads for low-cost and efficient dye-sensitized solar cells

2021 ◽  
Vol 45 (5) ◽  
pp. 2470-2477
Author(s):  
P. Golvari ◽  
E. Nouri ◽  
N. Mohsenzadegan ◽  
M. R. Mohammadi ◽  
S. O. Martinez-Chapa
Keyword(s):  
Light Scattering ◽  
Low Cost ◽  
Dye Sensitized Solar Cells ◽  
Single Layer ◽  
Cost Effective ◽  
Mesoporous Tio2 ◽  
Trap States ◽  
Dye Sensitized ◽  
Layer Deposition ◽  
Sensitized Solar Cells

Cost-effective DSCs with superior electronic properties are gained by a reduction in electronic trap states and outstanding light scattering and harvesting.

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