scholarly journals Ecological and microbiological diversity of chigger mites, including vectors of scrub typhus, on small mammals across stratified habitats in Thailand

2019 ◽  
Author(s):  
Kittipong Chaisiri ◽  
A. Christina Gill ◽  
Alexandr A. Stekolnikov ◽  
Soawapak Hinjoy ◽  
John W. McGarry ◽  
...  
Keyword(s):  
Species Richness ◽  
Scrub Typhus ◽  
Bacterial Pathogen ◽  
Febrile Illness ◽  
Asia Pacific ◽  
Habitat Types ◽  
Widespread Species ◽  
Human Land Use ◽  
Β Diversity ◽  
Α Diversity

AbstractScrub typhus, caused by a bacterial pathogen (Orientia spp.), is a potentially life-threatening febrile illness widely distributed in the Asia-Pacific region and is emerging elsewhere. The infection is transmitted by the larval stage of trombiculid mites (“chiggers”) that often exhibit low host specificity. Here, we present an analysis of chigger ecology for 38 species sampled from 11 provinces of Thailand and microbiomes for eight widespread species. In total, >16 000 individual chiggers were collected from 1 574 small mammal specimens belonging to 18 species across four horizontally-stratified habitat types. Chigger species richness was positively associated with higher latitudes, dry seasonal conditions, and host maturity; but negatively associated with increased human land use. Human scrub typhus incidence was found to be positively correlated with chigger species richness. The bacterial microbiome of chiggers was highly diverse, with Sphingobium, Mycobacterium, Neisseriaceae and various Bacillales representing the most abundant taxa. Only Leptotrombidium deliense was found to be infected with Orientia. β-diversity, but not α-diversity, was significantly different between chigger species and geographic regions, although not between habitat types. This first field survey of the chigger microbiome provides a framework for future studies on interactions between pathogens and other symbionts in these understudied vectors.

scholarly journals Ecological and microbiological diversity of chigger mites, including vectors of scrub typhus, on small mammals across stratified habitats in Thailand

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Kittipong Chaisiri ◽  
A. Christina Gill ◽  
Alexandr A. Stekolnikov ◽  
Soawapak Hinjoy ◽  
John W. McGarry ◽  
...  
Keyword(s):  
Species Richness ◽  
Scrub Typhus ◽  
Bacterial Pathogen ◽  
Febrile Illness ◽  
Asia Pacific ◽  
Habitat Types ◽  
Widespread Species ◽  
Human Land Use ◽  
Β Diversity ◽  
Α Diversity

Abstract Background Scrub typhus, caused by a bacterial pathogen (Orientia spp.), is a potentially life-threatening febrile illness widely distributed in the Asia-Pacific region and is emerging elsewhere. The infection is transmitted by the larval stage of trombiculid mites (“chiggers”) that often exhibit low host specificity. Here, we present an analysis of chigger ecology for 38 species sampled from 11 provinces of Thailand and microbiomes for eight widespread species. Results In total, > 16,000 individual chiggers were collected from 1574 small mammal specimens belonging to 18 species across four horizontally-stratified habitat types. Chigger species richness was positively associated with higher latitudes, dry seasonal conditions, and host maturity; but negatively associated with increased human land use. Human scrub typhus incidence was found to be positively correlated with chigger species richness. The bacterial microbiome of chiggers was highly diverse, with Sphingobium, Mycobacterium, Neisseriaceae and various Bacillales representing the most abundant taxa. Only Leptotrombidium deliense was found to be infected with Orientia and another potential pathogen, Borrelia spp., was frequently detected in pools of this species. β-diversity, but not α-diversity, was significantly different between chigger species and geographic regions, although not between habitat types. Conclusion Our study identified several key environmental and host-derived correlates of chigger species richness across Thailand, which in turn impacted on human scrub typhus incidence. Moreover, this first extensive field survey of the chigger microbiome revealed species- and province-level variation in microbial β-diversity across the country, providing a framework for future studies on interactions between pathogens and other symbionts in these understudied vectors.

scholarly journals Arthropod community structure in pastures of an island archipelago (Azores): looking for local–regional species richness patterns at fine-scales

2004 ◽  
Vol 94 (2) ◽  
pp. 111-121 ◽  
Author(s):  
P.A.V. Borges ◽  
V.K. Brown
Keyword(s):  
Species Richness ◽  
Regional Scale ◽  
Local Scale ◽  
Local Species Richness ◽  
Β Diversity ◽  
Arthropod Species ◽  
Α Diversity ◽  
Local Species ◽  
Richness Patterns

AbstractThe arthropod species richness of pastures in three Azorean islands was used to examine the relationship between local and regional species richness over two years. Two groups of arthropods, spiders and sucking insects, representing two functionally different but common groups of pasture invertebrates were investigated. The local–regional species richness relationship was assessed over relatively fine scales: quadrats (= local scale) and within pastures (= regional scale). Mean plot species richness was used as a measure of local species richness (= α diversity) and regional species richness was estimated at the pasture level (= γ diversity) with the ‘first-order-Jackknife’ estimator. Three related issues were addressed: (i) the role of estimated regional species richness and variables operating at the local scale (vegetation structure and diversity) in determining local species richness; (ii) quantification of the relative contributions of α and β diversity to regional diversity using additive partitioning; and (iii) the occurrence of consistent patterns in different years by analysing independently between-year data. Species assemblages of spiders were saturated at the local scale (similar local species richness and increasing β-diversity in richer regions) and were more dependent on vegetational structure than regional species richness. Sucking insect herbivores, by contrast, exhibited a linear relationship between local and regional species richness, consistent with the proportional sampling model. The patterns were consistent between years. These results imply that for spiders local processes are important, with assemblages in a particular patch being constrained by habitat structure. In contrast, for sucking insects, local processes may be insignificant in structuring communities.

scholarly journals Scrub typhus: a hospital-based study in the northern districts of West Bengal, India

2019 ◽  
Vol 7 (6) ◽  
pp. 2403
Author(s):  
Sanjay Kumar Mallick ◽  
Santanu Hazra ◽  
Tanmoy Nandi ◽  
Arunabha Sarkar
Keyword(s):  
Case Fatality Rate ◽  
West Bengal ◽  
Scrub Typhus ◽  
Case Fatality ◽  
Febrile Illness ◽  
Asia Pacific ◽  
Enzyme Linked Immunosorbent Assay ◽  
Acute Febrile Illness ◽  
Fatality Rate ◽  
Serological Tests

Background: Scrub typhus caused by Orientia tsutsugamushi, is a mite-borne zoonotic acute febrile illness. Geographically, it is confined to the Asia-Pacific region and important re-emerging infection in India. Clinical diagnosis of scrub typhus from other acute febrile illness is very difficult due to nonspecific symptoms and the relative absence of eschar in the Indian population. Case fatality rate varies from 30-70% depending on the clinical suspicion, delay in diagnosis and treatment. Antibody-based serological tests are the mainstay of diagnosis. IgM enzyme-linked immunosorbent assay (ELISA) against O. tsutsugamushi is helpful for the diagnosis of scrub typhus within the first week of illness.Methods: The aim of the study was to determine the prevalence of the disease in Northern districts of West Bengal, India using IgM ELISA.Results: Out of 577 serum samples tested 10.05% were positive for IgM antibodies. Majority of cases were below 40 years of age with higher prevalence in female patients. The disease showed a seasonal trend with a peak during the monsoon and later months. The case fatality rate among ELISA positive cases was 32.76%.Conclusions: Significant seropositivity against scrub typhus among cases of acute febrile illness with relatively higher mortality indicates that scrub typhus should be included in the differential diagnosis and confirmed by IgM ELISA.

scholarly journals Increases in local richness (α-diversity) following invasion are offset by biotic homogenization in a biodiversity hotspot

2019 ◽  
Vol 15 (5) ◽  
pp. 20190133 ◽  
Author(s):  
Alessandra R. Kortz ◽  
Anne E. Magurran
Keyword(s):  
Invasive Species ◽  
Species Richness ◽  
Biodiversity Hotspot ◽  
Contemporary World ◽  
Local Species Richness ◽  
Β Diversity ◽  
Α Diversity ◽  
Local Species ◽  
Biodiversity Change ◽  
Null Expectation

The world's ecosystems are experiencing unparalleled rates of biodiversity change, with invasive species implicated as one of the drivers that restructure local assemblages. Here we focus on the processes leading to biodiversity change in a biodiversity hotspot, the Brazilian Cerrado. The null expectation that invasion leads to increase in local species richness is supported by our investigation of the grass layer in two key habitats (campo sujo and campo úmido). Our analysis uncovered a linear relationship between total richness and invasive richness at the plot level. However, because the invasive species—even though few in number—are widespread, their contribution to local richness (α-diversity) is offset by their homogenizing influence on composition (β-diversity). We thus identify a mechanism that can help explain the paradox that species richness is not declining in many local assemblages, yet compositional change is exceeding the predictions of ecological theory. As such, our results emphasize the importance of quantifying both α-diversity and β-diversity in assessments of biodiversity change in the contemporary world.

scholarly journals Use of Multiplex Real-Time PCR To Diagnose Scrub Typhus

2017 ◽  
Vol 55 (5) ◽  
pp. 1377-1387 ◽  
Author(s):  
Wiwit Tantibhedhyangkul ◽  
Ekkarat Wongsawat ◽  
Saowaluk Silpasakorn ◽  
Duangdao Waywa ◽  
Nuttawut Saenyasiri ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Scrub Typhus ◽  
Febrile Illness ◽  
Asia Pacific ◽  
Diagnostic Tools ◽  
Pcr Assay ◽  
Content Type ◽  
Serologic Tests

ABSTRACTScrub typhus, caused byOrientia tsutsugamushi, is a common cause of acute undifferentiated febrile illness in the Asia-Pacific region. However, its nonspecific clinical manifestation often prevents early diagnosis. We propose the use of PCR and serologic tests as diagnostic tools. Here, we developed a multiplex real-time PCR assay using hydrolysis (TaqMan) probes targetingO. tsutsugamushi47-kDa,groEL, and human interferon beta (IFN-β gene) genes to improve early diagnosis of scrub typhus. The amplification efficiency was higher than 94%, and the lower detection limit was 10 copies per reaction. We used a human gene as an internal DNA quality and quantity control. To determine the sensitivity of this PCR assay, we selected patients with confirmed scrub typhus who exhibited a clear 4-fold increase in the level of IgG and/or IgM. The PCR assay result was positive in 45 of 52 patients, indicating a sensitivity of 86.5% (95% confidence interval [CI]: 74.2 to 94.4). The PCR assessment was negative for all 136 non-scrub typhus patients, indicating a specificity of 100% (95% CI: 97.3 to 100). In addition, this test helped diagnose patients with inconclusive immunofluorescence assay (IFA) results and using single blood samples. In conclusion, the real-time PCR assay proposed here is sensitive and specific in diagnosing scrub typhus. Combining PCR and serologic tests will improve the diagnosis of scrub typhus among patients presenting with acute febrile illness.

Holy or Not, Israel is a Palaearctic Biodiversity Hotspot

2011 ◽  
Vol 57 (3) ◽  
pp. 207-211
Author(s):  
Yoni Gavish
Keyword(s):  
Species Richness ◽  
Species Turnover ◽  
Grid Cell ◽  
Biodiversity Hotspot ◽  
Statistical Prediction ◽  
Β Diversity ◽  
Α Diversity ◽  
Species Area ◽  
Prediction Limits ◽  
Global Biodiversity

In their rebuttal to my comment, Roll et al. (2011) defend their original conclusion, by questioning the theoretical framework on which I based my analysis. They stress the importance of the statistical prediction limits and the treatment of latitudinal location as a covariate. They also add an additional grid-cell-based analysis. Here, I claim that even if provincial species-area relationships (SPAR) are not parallel, they are still different. While relying on Roll et al.'s (2011) analyses, I show that for each taxon there is at least one other provincial SPAR that lies considerably above the Palaearctic SPAR, making Palaearctic countries less favorable to be identified as a global biodiversity hotspot. I further claim that prediction limits should not be used to answer the question in focus and that adding latitude as a covariate does not alter the results. Finally, I address the grid-cell analyses of Roll et al. (2011), claiming that Israel's diversity lies mainly in the species turnover between cells (i.e., β diversity) and not on the average species richness within cells (α diversity). Therefore I hold on to my former conclusion that at least for three taxa—birds, mammals, and reptiles—Israel is indeed a Palaearctic provincial hotspot.

scholarly journals α- and β-Diversity Patterns of Macrophytes and Freshwater Fishes are Driven by Different Factors and Processes in Lakes of the Unexplored Southern Balkan Biodiversity Hotspot

Water ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1984
Author(s):  
Anthi Oikonomou ◽  
Konstantinos Stefanidis
Keyword(s):  
Species Richness ◽  
Species Turnover ◽  
Biodiversity Hotspot ◽  
Environmental Drivers ◽  
Conservation Strategies ◽  
Compositional Variation ◽  
Related Factors ◽  
Diversity Patterns ◽  
Β Diversity ◽  
Α Diversity

Disentangling the main drivers of species richness and community composition is a central theme in ecology. Freshwater biodiversity patterns have been poorly explored; yet, it has been shown that different freshwater biota have different, often contrasting responses to environmental gradients. In this study, we investigated the relative contribution of geographical and environmental (habitat-, climate- and water quality-related) factors/gradients in shaping the α- and β-diversity patterns of macrophytes and fish in sixteen natural freshwater lakes of an unexplored Balkan biodiversity hotspot, the Southern Balkan Peninsula. We employed generalized linear modeling to identify drivers of α-diversity, and generalized dissimilarity modeling to explore commonalities and dissimilarities of among-biota β-diversity. Species richness of both biota was significantly associated with lake surface area, whereas macrophytes had an inverse response to altitude, compared to fish. Both species turnover and nestedness significantly contributed to the total β-diversity of macrophytes. In contrast, species turnover was the most significant contributor to the total fish β-diversity. We found that the compositional variation of macrophytes is primarily limited by dispersal and ultimately shaped by environmental drivers, resulting in spatially structured assemblages. Fish communities were primarily shaped by altitude, highlighting the role of species sorting. We conclude that among-biota diversity patterns are shaped by different/contrasting factors, and, thus, effective/sustainable conservation strategies should encompass multiple aquatic biota.

scholarly journals Variation of plant species richness at different spatial scales

2018 ◽  
Vol 11 ◽  
pp. 49-62 ◽  
Author(s):  
Khem Raj Bhattarai
Keyword(s):  
Species Richness ◽  
Spatial Scales ◽  
Local Scale ◽  
Explanatory Variables ◽  
Local Species Richness ◽  
Β Diversity ◽  
Historical Processes ◽  
Α Diversity ◽  
Local Species ◽  
Broad Scale

 It is now realized that the variation in species richness is influenced by spatial and temporal scales. Pattern and scale are a central focus in ecology and biogeography. The species richness relationship depends on the scale of study and their correlated factors. The broad objective of this review is to elucidate how different scales are correlated with different explanatory variables to generate patterns of species richness. Addressing the problem of scale has both fundamental and applied importance in understanding variation in species richness along gradients. The understanding of pattern, its causes, and consequences is central to our understanding of processes such as succession, community development, and the spread and persistence of species. According to the hierarchical theory of species diversity there are mainly three categories of scales: local, landscape and regional. The local species richness or α-diversity is the diversity of individual stands. The β-diversity or species change is turnover between two elevational bands or between two plots or two sites. The regional or γ-diversity is the total richness of whole mountains or study systems and it has a combined influence from α- and β-diversity. The local species richness is affected by both local-scale processes (e.g., internal interactions) and broad-scale processes (e.g., evolutionary). Different explanatory variables according to the scales of study are necessary to explain variation at different spatial scales. Local factors (e.g., disturbance, grazing and tree cover) have been used to detect variation at a local scale. Generally, topographical factors are used to detect variation in species richness at a landscape scale; whereas climate, water-energy dynamics and historical processes are used to detect variation at a regional scale. However, it is not easy to separate strictly one scale from other because there is no clear boundary between them. The study of the whole elevation gradient from tropical to alpine zone or long latitude is a broad-scale study. The intermediate scale is a study on a local mountain, which covers the subtropical to warm temperate zones. To explain patterns of species richness, a pluralistic body of hypotheses, which incorporates historical, biological and climatic factors, is needed. This is depicted by the strong relationship between climate, biological interactions, and historical processes in influencing variation in species richness at different spatial scales.Botanica Orientalis – Journal of Plant Science (2017) 11: 49–62

scholarly journals Spatial scale changes the relationship between beta diversity, species richness and latitude

2018 ◽  
Vol 5 (9) ◽  
pp. 181168 ◽  
Author(s):  
Rachakonda Sreekar ◽  
Masatoshi Katabuchi ◽  
Akihiro Nakamura ◽  
Richard T. Corlett ◽  
J. W. Ferry Slik ◽  
...  
Keyword(s):  
Species Richness ◽  
Spatial Scale ◽  
Large Scale ◽  
Spatial Scales ◽  
Asia Pacific ◽  
Β Diversity ◽  
Sample Representativeness ◽  
Tree Communities ◽  
Tree Data ◽  
The Relationship

The relationship between β-diversity and latitude still remains to be a core question in ecology because of the lack of consensus between studies. One hypothesis for the lack of consensus between studies is that spatial scale changes the relationship between latitude and β-diversity. Here, we test this hypothesis using tree data from 15 large-scale forest plots (greater than or equal to 15 ha, diameter at breast height ≥ 1 cm) across a latitudinal gradient (3–30 o ) in the Asia-Pacific region. We found that the observed β-diversity decreased with increasing latitude when sampling local tree communities at small spatial scale (grain size ≤0.1 ha), but the observed β-diversity did not change with latitude when sampling at large spatial scales (greater than or equal to 0.25 ha). Differences in latitudinal β-diversity gradients across spatial scales were caused by pooled species richness (γ-diversity), which influenced observed β-diversity values at small spatial scales, but not at large spatial scales. Therefore, spatial scale changes the relationship between β-diversity, γ-diversity and latitude, and improving sample representativeness avoids the γ-dependence of β-diversity.

scholarly journals Diversity Patterns of Different Life Forms of Plants along an Elevational Gradient in Crete, Greece

Diversity ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 200 ◽  
Author(s):  
Maria Lazarina ◽  
Athanasios Charalampopoulos ◽  
Maria Psaralexi ◽  
Nikos Krigas ◽  
Danai-Eleni Michailidou ◽  
...  
Keyword(s):  
Species Richness ◽  
Species Composition ◽  
Large Scale ◽  
Regional Scale ◽  
Elevational Gradient ◽  
Life Forms ◽  
Species Pool ◽  
Diversity Patterns ◽  
Β Diversity ◽  
Α Diversity

Elevational gradients provide a unique opportunity to explore species responses to changing environmental conditions. Here, we focus on an elevational gradient in Crete, a climate-vulnerable Mediterranean plant biodiversity hotspot and explore the diversity patterns and underlying mechanisms of different plant life forms. We found that the significant differences in life forms’ elevational and environmental ranges are reflected in α- diversity (species richness at local scale), γ-diversity (species richness at regional scale) and β-diversity (variation in species composition). The α- and γ-diversity decreased with elevation, while β-diversity followed a hump-shaped relationship, with the peak varying between life forms. However, β-deviation (deviation from null expectations) varied significantly with elevation but was life formindependent. This suggests that species composition is shaped by the size of the available species pool which depends on life form, but also by other deterministic or stochastic processes that act in a similar way for different life forms. The strength of these processes varies with elevation, with hotter–drier conditions and increased human activities filtering species composition at lowlands and large-scale processes determining the species pool size overriding local ecological processes at higher elevations.

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