scholarly journals BDNF/TrkB.T1 signaling is a novel mechanism for astrocyte morphological maturation

2019 ◽  
Author(s):  
Leanne M. Holt ◽  
Natasha L. Pacheco ◽  
Raymundo Hernandez ◽  
Muhannah Hossain ◽  
Michelle L. Olsen
Keyword(s):  
Growth Factor ◽  
Neurotrophic Factor ◽  
Neuronal Morphology ◽  
Cns Development ◽  
Protein Coding ◽  
Morphological Complexity ◽  
Mature Astrocyte ◽  
Morphological Maturation ◽  
Critical Process

AbstractBrain derived neurotrophic factor (BDNF) is a critical growth factor involved in the maturation of neurons, including neuronal morphology and synapse refinement. Herein, we demonstrate astrocytes express high levels of BDNF’s receptor, TrkB (in the top 20 of protein-coding transcripts), with nearly exclusive expression of the truncated isoform, TrkB.T1 which peaks in expression during astrocyte morphological maturation. Using a novel culture paradigm, we show that astrocyte morphological complexity is increased in the presence of BDNF and is dependent upon BDNF/TrkB.T1 signaling. Deletion of TrkB.T1 in vivo revealed morphologically immature astrocytes with significantly reduced volume and branching, as well as dysregulated expression of perisynaptic genes associated with mature astrocyte functions, including synaptogenic genes. Indicating a role for functional astrocyte maturation via BDNF/TrkB.T1 signaling, TrkB.T1 KO astrocytes do not support normal excitatory synaptogenesis. These data suggest a significant role for BDNF/TrkB.T1 signaling in astrocyte morphological maturation, a critical process for CNS development.

scholarly journals Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Leanne M Holt ◽  
Raymundo D Hernandez ◽  
Natasha L Pacheco ◽  
Beatriz Torres Ceja ◽  
Muhannah Hossain ◽  
...  
Keyword(s):  
Growth Factor ◽  
Neurotrophic Factor ◽  
Neuronal Morphology ◽  
Cns Development ◽  
Protein Coding ◽  
Morphological Complexity ◽  
Mature Astrocyte ◽  
Bdnf Signaling ◽  
Morphological Maturation ◽  
Critical Process

Brain-derived neurotrophic factor (BDNF) is a critical growth factor involved in the maturation of the CNS, including neuronal morphology and synapse refinement. Herein, we demonstrate astrocytes express high levels of BDNF’s receptor, TrkB (in the top 20 of protein-coding transcripts), with nearly exclusive expression of the truncated isoform, TrkB.T1, which peaks in expression during astrocyte morphological maturation. Using a novel culture paradigm, we show that astrocyte morphological complexity is increased in the presence of BDNF and is dependent upon BDNF/TrkB.T1 signaling. Deletion of TrkB.T1, globally and astrocyte-specifically, in mice revealed morphologically immature astrocytes with significantly reduced volume, as well as dysregulated expression of perisynaptic genes associated with mature astrocyte function. Indicating a role for functional astrocyte maturation via BDNF/TrkB.T1 signaling, TrkB.T1 KO astrocytes do not support normal excitatory synaptogenesis or function. These data suggest a significant role for BDNF/TrkB.T1 signaling in astrocyte morphological maturation, a critical process for CNS development.

Vascular endothelial growth factor C acts as a neurotrophic factor for dopamine neurons in vitro and in vivo

Neuroscience ◽  
2011 ◽  
Vol 192 ◽  
pp. 550-563 ◽  
Author(s):  
M. Piltonen ◽  
A. Planken ◽  
O. Leskelä ◽  
T.T. Myöhänen ◽  
A.-L. Hänninen ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Neurotrophic Factor ◽  
Dopamine Neurons ◽  
Vascular Endothelial ◽  
Factor C ◽  
Endothelial Growth Factor
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