scholarly journals Reference genome for the WHO reference strain for Mycobacterium bovis BCG Danish, the present tuberculosis vaccine

2019 ◽  
Author(s):  
Katlyn Borgers ◽  
Jheng-Yang Ou ◽  
Po-Xing Zheng ◽  
Petra Tiels ◽  
Annelies Van Hecke ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Vaccine Strain ◽  
Genome Analysis ◽  
Reference Strain ◽  
Reference Genome ◽  
Bacillus Calmette Guerin ◽  
Genome Duplications ◽  
Analysis Workflow ◽  
Derivatives Of ◽  
Reference Strains

ABSTRACTMycobacterium bovis bacillus Calmette-Guérin (M. bovis BCG) is the only vaccine available against tuberculosis (TB). This study reports on an integrated genome analysis workflow for BCG, resulting in the completely assembled genome sequence of BCG Danish 1331 (07/270), one of the WHO reference strains for BCG vaccines. We demonstrate how this analysis workflow enables the resolution of genome duplications and of the genome of engineered derivatives of this vaccine strain.

scholarly journals Genome analysis of a MDR Streptococcus pneumoniae 23F serotype causing meningoencephalitis in a 10-months refugee infant

2018 ◽  
Vol 12 (03) ◽  
pp. 196-203
Author(s):  
Tamara Salloum ◽  
Elie Tannous ◽  
Samar Merheb-Ghoussoub ◽  
Elie Ghoussoub ◽  
Sima Tokajian
Keyword(s):  
Streptococcus Pneumoniae ◽  
Virulence Factors ◽  
Genome Analysis ◽  
Reference Strain ◽  
Clinical Diagnostics ◽  
Penicillin Binding Proteins ◽  
Multiple Components ◽  
Genes Encoding ◽  
Resistant Strains ◽  
Reference Strains

Purpose: Streptococcus pneumoniae is an important human pathogen causing invasive pneumococcal diseases (IPD). The re-emergence of eradicated S. pneumoniae-associated meningoencephalitis in Lebanon is a major point of concern. Methods: We aimed at conducting a comparative genome analysis of a multi-drug resistant S. pneumoniae, LAU-23F, linked to meningoencephalitis and fatality in a 10-months Syrian refugee infant in Lebanon, and 24 related publically available genome sequences. Serotype, capsular genes, MLST, SNPs, phylogenetic relatedness and repertoire of resistance genes were investigated. Genes encoding penicillin binding proteins (PBPs) were examined for mosaicity. Virulence factors were screened for SNPs as compared to reference strains. Results: The isolate belonged to ST-277 and was of serotype 23F. It showed an intermediate resistance to ciprofloxacin, cefuroxime and penicillin and carried multiple components of different efflux pumps. Gene mosaicity was observed in pbp2x, it was also distinct from other penicillin-resistant strains; pbp1a and pbp2b appeared to be conserved between LAU-23F and the reference strain SP49. The arrangement of capsular gene loci was similar to ATCC 700669 though polymorphism was detected in the cpsABCD region, believed to be conserved among different Streptococcus species. Amplitude of virulence factors was detected showing varying degrees of conservation compared to reference strains. Observed zones of high heterogeneity were associated with phage encoded regions. Conclusions: The fine levels of diversity throughout the genome could account for the pronounced invasiveness of this isolate. The genomics-based methods used support the importance of implementing WGS in routine clinical diagnostics and surveillance of streptococcal diseases.

scholarly journals Updated functional annotation of the Mycobacterium bovis AF2122/97 reference genome

2019 ◽  
Author(s):  
Damien Farrell ◽  
Joseph Crispell ◽  
Stephen V. Gordon
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Genome Annotation ◽  
Functional Annotation ◽  
Reference Strain ◽  
Reference Genome ◽  
Coding Sequences ◽  
New Gene ◽  
New Protein

AbstractMycobacterium bovis AF2122/97 is the reference strain for the bovine tuberculosis bacillus. We here report an update to the M. bovis AF2122/97 genome annotation to reflect 616 new protein identifications which replace many of the old hypothetical coding sequences and proteins of unknown function in the genome. These changes integrate information from functional assignments of orthologous coding sequences in the Mycobacterium tuberculosis H37Rv genome. We have also added 69 additional new gene names.

scholarly journals Updated functional annotation of the Mycobacterium bovis AF2122/97 reference genome

2020 ◽  
Vol 2 (7) ◽  
Author(s):  
Damien Farrell ◽  
Joseph Crispell ◽  
Stephen V. Gordon
Keyword(s):  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Type Species ◽  
Reference Strain ◽  
Reference Genome ◽  
Coding Sequences ◽  
Content Type ◽  
Link Type ◽  
New Gene ◽  
New Protein

Mycobacterium bovis AF2122/97 is the reference strain for the bovine tuberculosis bacillus. Here we report an update to the M. bovis AF2122/97 genome annotation to reflect 616 new protein identifications that replace many of the old hypothetical coding sequences and proteins of unknown function in the genome. These changes integrate information from functional assignments of orthologous coding sequences in the Mycobacterium tuberculosis H37Rv genome. We have also added 69 additional new gene names.

Mycobacterium bovis Bacillus Calmette-Guérin Killed by Extended Freeze-Drying Reduces Colitis in Mice

2011 ◽  
Vol 141 (2) ◽  
pp. 642-652.e4 ◽  
Author(s):  
Micheline Lagranderie ◽  
Christoph Kluge ◽  
Helene Kiefer–Biasizzo ◽  
Mohammad Abolhassani ◽  
Marie–Anne Nahori ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Freeze Drying ◽  
Bacillus Calmette Guerin

scholarly journals Impact of in vitro evolution on antigenic diversity of Mycobacterium bovis bacillus Calmette-Guerin (BCG)

Vaccine ◽  
2014 ◽  
Vol 32 (45) ◽  
pp. 5998-6004 ◽  
Author(s):  
Richard Copin ◽  
Mireia Coscollá ◽  
Efstratios Efstathiadis ◽  
Sebastien Gagneux ◽  
Joel D. Ernst
Keyword(s):  
Mycobacterium Bovis ◽  
Bacillus Calmette Guerin ◽  
In Vitro Evolution ◽  
Antigenic Diversity

scholarly journals The double-sided effects of Mycobacterium Bovis bacillus Calmette–Guérin vaccine

npj Vaccines ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Junli Li ◽  
Lingjun Zhan ◽  
Chuan Qin
Keyword(s):  
Literature Review ◽  
Experimental Evidence ◽  
Mycobacterium Bovis ◽  
Virus Disease ◽  
Bcg Vaccine ◽  
Bacillus Calmette Guerin ◽  
Mycobacterial Infections ◽  
Specific Effects ◽  
Mycobacterial Diseases ◽  
Protection Mechanisms

AbstractBacillus Calmette–Guérin (BCG), the only vaccine proven to be effective against tuberculosis (TB), is the most commonly used vaccine globally. In addition to its effects on mycobacterial diseases, an increasing amount of epidemiological and experimental evidence accumulated since its introduction in 1921 has shown that BCG also exerts non-specific effects against a number of diseases, such as non-mycobacterial infections, allergies and certain malignancies. Recent Corona Virus Disease 2019 (COVID-19) outbreak has put BCG, a classic vaccine with significant non-specific protection, into the spotlight again. This literature review briefly covers the diverse facets of BCG vaccine, providing new perspectives in terms of specific and non-specific protection mechanisms of this old, multifaceted, and controversial vaccine.

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