scholarly journals Human Cytomegalovirus Genomes Sequenced Directly from Clinical Material: Variation, Multiple-Strain Infection, Recombination and Mutation

2018 ◽  
Author(s):  
Nicolás M. Suárez ◽  
Gavin S. Wilkie ◽  
Elias Hage ◽  
Salvatore Camiolo ◽  
Marylouisa Holton ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
High Throughput Sequencing ◽  
Clinical Pathology ◽  
Congenital Infection ◽  
Population Based ◽  
Single Strain ◽  
Natural Mutation ◽  
Future Population

ABSTRACTThe genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination and natural mutation. A total of 207 datasets generated in this and previous studies using target enrichment and high-throughput sequencing were analysed, in the process facilitating the determination of genome sequences for 91 strains. Key findings were that (i) it is important to monitor the quality of sequencing libraries in investigating diversity, (ii) many recombinant strains have been transmitted during HCMV evolution, and some have apparently survived for thousands of years without further recombination, (iii) mutants with non-functional genes (pseudogenes) have been circulating and recombining for long periods and can cause congenital infection and resulting clinical sequelae, and (iv) intrahost diversity in single-strain infections is much less than that in multiple-strain infections. Future population-based studies are likely to continue illuminating the evolution, epidemiology and pathogenesis of HCMV.

scholarly journals Human Cytomegalovirus Genomes Sequenced Directly From Clinical Material: Variation, Multiple-Strain Infection, Recombination, and Gene Loss

2019 ◽  
Vol 220 (5) ◽  
pp. 781-791 ◽  
Author(s):  
Nicolás M Suárez ◽  
Gavin S Wilkie ◽  
Elias Hage ◽  
Salvatore Camiolo ◽  
Marylouisa Holton ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
High Throughput Sequencing ◽  
Gene Loss ◽  
Clinical Pathology ◽  
Congenital Infection ◽  
Population Based ◽  
Single Strain ◽  
Future Population

AbstractThe genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total of 207 datasets generated in this and previous studies using target enrichment and high-throughput sequencing were analyzed, in the process enabling the determination of genome sequences for 91 strains. Key findings were that (i) it is important to monitor the quality of sequencing libraries in investigating variation; (ii) many recombinant strains have been transmitted during HCMV evolution, and some have apparently survived for thousands of years without further recombination; (iii) mutants with nonfunctional genes (pseudogenes) have been circulating and recombining for long periods and can cause congenital infection and resulting clinical sequelae; and (iv) intrahost variation in single-strain infections is much less than that in multiple-strain infections. Future population-based studies are likely to continue illuminating the evolution, epidemiology, and pathogenesis of HCMV.

scholarly journals Performance of self-reported measures of alcohol use and of harmful drinking patterns against ethyl glucuronide hair testing among young Swiss men

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244336
Author(s):  
Katia Iglesias ◽  
Séverine Lannoy ◽  
Frank Sporkert ◽  
Jean-Bernard Daeppen ◽  
Gerhard Gmel ◽  
...  
Keyword(s):  
Alcohol Use ◽  
Psychometric Properties ◽  
Empirical Studies ◽  
Population Based ◽  
Ethyl Glucuronide ◽  
Drinking Patterns ◽  
Hair Testing ◽  
Risky Single Occasion Drinking

Background There is a need for empirical studies assessing the psychometric properties of self-reported alcohol use as measures of excessive chronic drinking (ECD) compared to those of objective measures, such as ethyl glucuronide (EtG). Objectives To test the quality of self-reported measures of alcohol use and of risky single-occasion drinking (RSOD) to detect ECD assessed by EtG. Methods A total of 227 samples of hair from young Swiss men were used for the determination of EtG. Self-reported measures of alcohol use (previous twelve-month and previous-week alcohol use) and RSOD were assessed. Using EtG (<30 pg/mg) as the gold standard of ECD assessment, the sensitivity and specificity were computed, and the AUROC were compared for alcohol use measures and RSOD. Logistic regressions were used to test the contribution of RSOD to the understanding of ECD after controlling for alcohol use. Results A total of 23.3% of participants presented with ECD. Previous twelve-month alcohol use with a cut-off of >15 drinks per week (sensitivity = 75.5%, specificity = 78.7%) and weekly RSOD (sensitivity = 75.5%, specificity = 70.1%) yielded acceptable psychometric properties. No cut-off for previous-week alcohol use gave acceptable results. In the multivariate logistic regression, after controlling for the previous twelve months of alcohol use, RSOD was still significantly associated with EtG (p = .016). Conclusion Self-reported measures of the previous twelve months of alcohol use and RSOD were acceptable measures of ECD for population-based screening. Self-reported RSOD appeared to be an interesting screening measure, in addition to the previous twelve months of alcohol use, to understand ECD among young people.

Tingkat Pemahaman Kompetensi Guru Pada Mahasiswa Pendidikan Geografi Sebagai Calon Guru Geografi

2020 ◽  
Vol 21 (1) ◽  
pp. 102-117
Author(s):  
Novia Zalmita ◽  
Muhajirah Muhajirah ◽  
Abdul Wahab Abdi
Keyword(s):  
Prospective Teachers ◽  
Prospective Teacher ◽  
Quantitative Description ◽  
Population Data ◽  
Geography Education ◽  
Teacher Competence ◽  
Education Department ◽  
Long Time

One that influences human resource indicators is education. The teacher is a profession as a job of academic specialization in a relatively long time in college. Understanding related to teacher competence is very important to have by a prospective teacher because it can affect the quality of performance as a professional teacher. The teacher's competence is known as pedagogic, professional, social and personality competencies. The issue in this study is how the competency of the teacher of the Department of Geography Education FKIP Unsyiah as a prospective teacher of geography? The purpose of this study was to determine the competence of teachers in the Department of Geography Education FKIP Unsyiah as prospective geography teachers. Quantitative description approach is used in this study to find answers to the issue. The population in this study were students of the Department of Geography Education FKIP Unsyiah class of 2015 and 2016 who had been declared to have passed the Micro Teaching and Magang Kependidikan 3 course totaling 50 people. Because the population is small and can be reached, the determination of the sample using total sampling techniques so that the sample in this study is the whole population. Data collection is done by distributing test questions to respondents. The data was analyzed using the descriptive statistics percentage formula. The results of the study indicate that the level of teacher competence of Geography Education Department students as prospective teachers is in the moderate category, namely as many as 22 respondents (44%). A total of 12 respondents (24%) were in the high category, 15 respondents (30%) were in the low category and 1 respondent (2%) were in the very low category.

Fluctuations of Annual Precipitation and Water Resources Quality in Ukraine

2016 ◽  
Vol 10 (4s) ◽  
pp. 621-629
Author(s):  
Valentina Pidlisnyuk ◽  
◽  
John Harrington JR ◽  
Yulia Melnyk ◽  
Yuliya Vystavna ◽  
...  
Keyword(s):  
Water Resources ◽  
Surface Waters ◽  
Precipitation Amount ◽  
Ion Concentration ◽  
Annual Precipitation ◽  
Resource Quality ◽  
Phosphate Ion ◽  
Regional Trends

The article focuses on examining the influence of fluctuations in annual precipitation amount on the quality of surface waters. Water quality was estimated with data on BOD, COD and phosphate–ion concentration within five selected regions of Ukraine. Analysis of the precipitation data (1991 – 2010) showed different regional trends. Using the statistics, determination of the interconnection between precipitation amount and water resources quality were done. The obtained regularities and associated uncertainties can be used for prediction of changes in water resource quality and as a guide for future adaptation to possible climate change.

POTENSI PENGEMBANGAN SADE SEBAGAI DESA WISATA LOMBOK

2016 ◽  
Vol 20 (2) ◽  
pp. 69-82
Author(s):  
W. Suprihatin ◽  
H. Hailuddin
Keyword(s):  
Analytical Hierarchy Process ◽  
Environmental Aspect ◽  
Priority Level ◽  
Highest Weight ◽  
Local Tradition ◽  
Tourist Village ◽  
Hierarchy Process ◽  
Initial Pattern

The background of the problems in this study is the decreasing quality of Sade hamlet amid rising tourist arrivals. From the environmental aspect, the conditions of the hamlet began to decline, in which the initial pattern of Sade has started a lot of changes towards the deficient and began to leave the local tradition. One effort to improve the condition of Sade hamlet in social, cultural and the environmental aspect is through the formulation of a sustainable structuring, the presence and identity maintaining and making a sustainable Tourism Village. Through analysis of AHP (Analytical Hierarchy Process) by collecting the perceptions of some experts through interviews and questionnaires, obtained by weighting the priority of the experts, namely the preservation of culture as an element of priority-level goals to be achieved in the development of Sade Hamlet as a tourist village at 0,476. While the determination of the level of the main criteria in the achievement of these objectives is the highest weight while maintaining a typical village environment at 0.319. Priority strategies that get the highest weight of the experts is that Sade Hamlet Revitalization with a priority weighting of 0.583. The second priority is the relocation of Hamlet at 0.235. Lowest weighting or last priority is Replication Sade Hamlet at 0.182.

Automation of selected brazes' dynamic properties in high-temperatures' measurement process

2013 ◽  
Vol 18 (2-3) ◽  
pp. 33-41
Author(s):  
Dominik Sankowski ◽  
Marcin Bakala ◽  
Rafał Wojciechowski
Keyword(s):  
Surface Tension ◽  
High Temperatures ◽  
Dynamic Properties ◽  
Automatic Measurement ◽  
Measurement Methodology ◽  
Joining Process ◽  
Wetting Force ◽  
Research Grant

Abstract The good quality of several manufactured components frequently depends on solidliquid interactions existing during processing. Nowadays, the research in material engineering focuses also on modern, automatic measurement methods of joining process properties, i.a. wetting force and surface tension, which allows for quantitative determination of above mentioned parameters. In the paper, the brazes’ dynamic properties in high-temperatures’ measurement methodology and the stand for automatic determination of braze’s properties, constructed and implmented within the research grant nr KBN N N519 441 839 - An integrated platform for automatic measurement of wettability and surface tension of solders at high temperatures, are widely described

Assessing the quality of public health in the Russian Federation and Israel: Main problems and solutions

2020 ◽  
Vol 19 (4) ◽  
pp. 618-632
Author(s):  
A.S. Panchenko
Keyword(s):  
Public Health ◽  
Russian Federation ◽  
Population Based ◽  
The Public ◽  
Disability Adjusted Life ◽  
Problems And Solutions ◽  
The Russian Federation ◽  
Disability Adjusted Life Year ◽  
Practical Recommendations

Subject. The article addresses the public health in the Russian Federation and Israel. Objectives. The focus is on researching the state of public health in Russia and Israel, using the Global Burden of Disease (GBD) project methodology, identifying problem areas and searching for possible ways to improve the quality of health of the Russian population based on the experience of Israel. Methods. The study draws on the ideology of the GBD project, which is based on the Disability-Adjusted Life-Year (DALY) metric. Results. The paper reveals the main causes of DALY losses and important risk factors for cancer for Russia and Israel. The findings show that the total DALY losses for Russia exceed Israeli values. The same is true for cancer diseases. Conclusions. Activities in Israel aimed at improving the quality of public health, the effectiveness of which has been proven, can serve as practical recommendations for Russia. The method of analysis, using the ideology of the GBD project, can be used as a tool for quantitative and comparative assessment of the public health.

X-ray fluorescence determination of zinc sulfate in acidic zinc plating electrolyte

2020 ◽  
Vol 86 (10) ◽  
pp. 18-22
Author(s):  
K. N. Vdovin ◽  
K. G. Pivovarova ◽  
N. A. Feoktistov ◽  
T. B. Ponamareva
Keyword(s):  
Zinc Sulfate ◽  
Zinc Coating ◽  
Electrolyte Composition ◽  
Complexometric Titration ◽  
X Ray ◽  
Fluorescence Determination ◽  
Zinc Plating ◽  
Zinc Determination

Zinc sulfate is the main component in the composition of the acidic zinc plating electrolyte. Deviation in the electrolyte composition from the optimum content leads to destabilization of the electrolysis process and deteriorate the quality of the resulting zinc coating. The proper quality of a zinc coating obtained by galvanic deposition can be ensured only with timely monitoring and adjustment of the electrolyte composition. A technique of X-ray fluorescence determination of zinc (in terms of zinc sulfate) in an acidic zinc plating electrolyte is proposed. The study was carried out using an ARL Quant’X energy dispersive spectrometer (Thermo Fisher Scientific, USA) with a semiconductor silicon-lithium detector. The features of the spectrometer design are presented. The optimal parameters of excitation and detection of zinc radiation were specified when the electrolyte sample was diluted 1:1000. The ZnKα1 line was used as an analytical line. The plotted calibration graph is linear, the correlation coefficient being 0.999234. The results of zinc determination according to the developed method were compared with the data of the reference method of complexometric titration to prove the reliability of the procedure. The results are characterized by good convergence and accuracy. The proposed method of X-ray fluorescence zinc determination in a zinc plating electrolyte equals complexometric titration in the limiting capabilities and even exceeds the latter in terms of the simplicity of sample preparation and rapidity. The developed method of X-ray fluorescence determination of zinc is implemented in analysis of the electrolyte used in the continuous galvanizing unit at «METSERVIS LLC».

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