scholarly journals FilmArray Respiratory Panel Integrated in a field Point Of Care dispositive for the diagnosis of respiratory tract infections in rural areas in Senegal

2018 ◽  
Author(s):  
Hubert Bassene ◽  
Sophie Edouard ◽  
Georges Diatta ◽  
Jean Christophe Lagier ◽  
Oleg Mediannikov ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Rural Areas ◽  
Respiratory Tract Infections ◽  
Point Of Care ◽  
Influenza B Virus ◽  
Influenza B ◽  
Respiratory Pathogens ◽  
Field Point ◽  
Nasal Swabs ◽  
Tract Infections

The development of molecular syndrome-based kits for the diagnosis of respiratory infections offers rapid and sensitive detection of common respiratory pathogens and will have a significant impact on the care of patients. In this study, we present the results obtained after the introduction of the FilmArray respiratory panel in a field Point of care (POC) for the diagnosis of virus and bacteria responsible of respiratory tract infections in Senegal rural area. From February to August 2017, we collected nasal swabs from febrile patients that presented symptoms of respiratory tract infections in three health posts located in Niakhar. Specimens were tested on site by multiplex Polymerase Chain Reaction (PCR), using the FilmArray respiratory panel® that targets 20 pathogens, including 17 virus and 3 bacteria (bioMérieux). Nasal swabs were collected from 113 patients. The median age was 4 years (ranging from 4 months to 60 years) and 51 (45%) were males. The prevalence of respiratory pathogens was 37.5% (12/32) during the dry season and 54.3% (44/81) in the rainy season (p=0.16). The prevalence of respiratory pathogen carriage was higher in children under 5 years of age (38/55, 69.1%). The most prevalent micro-organisms detected were influenza B virus (16/113, 14%), human rhinovirus/enterovirus (10/113, 9%), parainfluenzae virus (9/113, 8%), respiratory syncytial virus (8/113, 7%), adenovirus (5/113, 4%), human metapneumovirus (3/113, 3%), Chlamydia pneumoniae (2/113, 2%) and Coronavirus (2/113, 2%). The study has demonstrated that the integration of the FilmArray respiratory panel into a field POC could significantly improve the management of respiratory tract infections in rural areas.

scholarly journals Multiplex Tests for Respiratory Tract Infections: The Direct Utility of the FilmArray Respiratory Panel in Emergency Department

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Siyuan Yang ◽  
Hui Li ◽  
Yunxia Tang ◽  
Fengting Yu ◽  
Chengjie Ma ◽  
...  
Keyword(s):  
Emergency Department ◽  
Respiratory Tract ◽  
Influenza A ◽  
Respiratory Tract Infections ◽  
Pathogen Detection ◽  
Point Of Care ◽  
Respiratory Pathogens ◽  
Point Of Care Testing ◽  
Tract Infections ◽  
First Time

Background. The FilmArray Respiratory Panel with multiplex targets for respiratory pathogens has been widely used and verified in clinical trials in special test settings. However, it is necessary to evaluate the panel’s performance at the point of care directly, in nonspecific test settings. Methods. Patients with respiratory tract infections were enrolled from among emergency department visitors, and all steps, including the collection of specimens and testing, were performed by our clinicians. Results. Among 270 patients, 196 (72.6%) patients were found to have one or more pathogens. For signal pathogen detection, influenza A virus had the highest rate of detection; 45 (16.7%) of the patients had two or more respiratory pathogens codetected, and most of the multiplex pathogens were rhinovirus/enterovirus codetected with Bordetella pertussis (17.8%). The information provided by the FilmArray had an impact on the prescription of antimicrobials, and there were differences in the rates of antibiotic prescriptions and anti-influenza prescriptions among patients. Conclusions. Use of the FilmArray by clinical staff was successfully implemented in the emergency department for the first time in China. The FilmArray has the potential for point-of-care testing in nonspecific settings.

scholarly journals Molecular Detection and Clinical Characteristics of Bacterial and Viral Main Etiological Agents Causing Respiratory Tract Infections in Tehran, Iran

2021 ◽  
Author(s):  
Taher Azimi Sarikhanbaghloo ◽  
Ramin Hamidi-Farahani ◽  
Ali Asgari ◽  
Jalil Rajabi ◽  
Mousa Ahmadi ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Military Personnel ◽  
Influenza A ◽  
Respiratory Tract Infections ◽  
Influenza B Virus ◽  
Influenza B ◽  
Pcr Method ◽  
Etiological Agents ◽  
Tract Infections ◽  
The Military

Abstract Background: Respiratory tract infections are one of the most important infections among military personals, worldwide. The present study aimed to survey the prevalence of bacterial and viral main etiological agents causing respiratory tract infections among the military personnel in Iran. A cross-sectional study was performed from September 2020 to February 2021. Nasopharyngeal swabs were taken from patients, military personnel with respiratory tract infection symptoms. Detection of COVID-19 was performed with one step qRT-PCR method. TaqMan probe-based real-time PCR assay was used for the detection of influenza A and B viruses. The prevalence of adenovirus and M. pneumoniae were determined using nested PCR. Moreover, B. pertussis and S. pyogenes were identified by conventional PCR assay. The detection of H. influenzae was performed by multiplex PCR method. Results: Overall, 145 patients were included. Among viral pathogens, COVID-19, influenza A virus, and adenovirus were identified in 85.5%, 4.1%, and 1.4% of patients, respectively. Influenza B virus was not detected among military personnel. The frequency of bacteria etiological agents was as follows: S. pyogenes (2%), M. pneumoniae (0.7%), H. influenzae (0%), and B. pertussis (0%). Muscle aches (75.9%), headache (70.3%), lethargy (69%), cough (66.2%), stuffy nose (56.6%), fever (53.8%), and sore throat (53.1%) were amongst the most common clinical symptoms. Conclusions: Results showed that the military personals are the susceptible group to COVID-19 infection. Therefore, the accurate detection and implementation of control strategies such as vaccination are necessary.

scholarly journals Multiplex tests for respiratory tract infections, direct utility of FilmArray respiratory panel in the emergency department

2020 ◽  
Author(s):  
Siyuan Yang ◽  
Hui Li ◽  
Yunxia Tang ◽  
Fengting Yu ◽  
Chengjie Ma ◽  
...  
Keyword(s):  
Emergency Department ◽  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Point Of Care ◽  
Winter Season ◽  
Diagnostic Methods ◽  
Respiratory Pathogens ◽  
Atypical Pathogens ◽  
B Virus ◽  
Tract Infections

Abstract BackgroundThe FilmArray respiratory panel with 20 targets for viral and atypical pathogens aimed at point of care tests, as multiplex tests for respiratory tract infections it has been widely used and verified in various clinical trials. The FilmArray respiratory panel (FilmArray RP) seems to be a reliable and rapid tool in special tests settings such as clinical laboratory, and it’s necessary to evaluate the panel’s performance in point of care directly with non-specific tests settings. MethodsIn this single-centre, open, observational study, we enrolled outpatients with respiratory tract infection from emergency department during 2016-2017 winter season, specimens from participants were detected using FilmArray RP. All steps including recruitment, collection of specimens and testing were performed by our clinical staff. The results of pathogens detected, clinical and demographic data were collected and analyzed. ResultsBetween October 2016 and May 2017, we enrolled 271 eligible patients, FilmArray RP was successful used in 270 patients: 196 (72.6 %) patients had been detected with one or more pathogens, 74 (27.4) patients got negative results; in the analysis of signal pathogen detected, influenza A virus had the highest rate of detection, and the rate in group ≤ 16 years, 16-49 years and ≥ 50 years was 22.8 %, 32.4 %, 31.1 % respectively; 45 (16.7 %) patients were co-detected with two or more respiratory pathogens, most multiplex pathogens were rhinovirus/enterovirus co-detected with Bordetella. pertussis (17.8 %). The outcomes provided by FilmArray RP had impact on the prescriptions of antimicrobials, there were difference in the rates of antibiotic prescriptions and anti-influenza prescriptions among patients with Flu A/B virus detected, non-Flu A/B virus pathogen detected and non-pathogen.ConclusionsThe performance of FilmArray RP using by clinical staffs was successfully implemented in emergency department for which is the first time in China. The multiplex diagnostic system significantly increased the rate of respiratory pathogens detected in diagnostic methods limited hospital. Despite this, FilmArray RP has a potential use as a tool for point of care testing in non-specific setting and operated by clinical staffs not in a laboratory.

Frequent respiratory pathogens of respiratory tract infections in children attending daycare centers

2011 ◽  
Vol 87 (5) ◽  
pp. 439-44 ◽  
Author(s):  
Caroline M. Bonfim ◽  
Maurício L. Nogueira ◽  
Paulo Vítor M. Simas ◽  
Luis Gustavo A. Gardinassi ◽  
Edison L. Durigon ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Respiratory Pathogens ◽  
Daycare Centers ◽  
Tract Infections

scholarly journals Point-of-Care C-Reactive Protein Testing to Reduce Antibiotic Prescribing for Respiratory Tract Infections in Primary Care: Systematic Review and Meta-Analysis of Randomised Controlled Trials

Antibiotics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 610
Author(s):  
Nahara Anani Martínez-González ◽  
Ellen Keizer ◽  
Andreas Plate ◽  
Samuel Coenen ◽  
Fabio Valeri ◽  
...  
Keyword(s):  
Primary Care ◽  
Respiratory Tract ◽  
Usual Care ◽  
Respiratory Tract Infections ◽  
Point Of Care ◽  
Antibiotic Prescribing ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Meta Analyses ◽  
Tract Infections

C-reactive protein (CRP) point-of-care testing (POCT) is increasingly being promoted to reduce diagnostic uncertainty and enhance antibiotic stewardship. In primary care, respiratory tract infections (RTIs) are the most common reason for inappropriate antibiotic prescribing, which is a major driver for antibiotic resistance. We systematically reviewed the available evidence on the impact of CRP-POCT on antibiotic prescribing for RTIs in primary care. Thirteen moderate to high-quality studies comprising 9844 participants met our inclusion criteria. Meta-analyses showed that CRP-POCT significantly reduced immediate antibiotic prescribing at the index consultation compared with usual care (RR 0.79, 95%CI 0.70 to 0.90, p = 0.0003, I2 = 76%) but not during 28-day (n = 7) follow-up. The immediate effect was sustained at 12 months (n = 1). In children, CRP-POCT reduced antibiotic prescribing when CRP (cut-off) guidance was provided (n = 2). Meta-analyses showed significantly higher rates of re-consultation within 30 days (n = 8, 1 significant). Clinical recovery, resolution of symptoms, and hospital admissions were not significantly different between CRP-POCT and usual care. CRP-POCT can reduce immediate antibiotic prescribing for RTIs in primary care (number needed to (NNT) for benefit = 8) at the expense of increased re-consultations (NNT for harm = 27). The increase in re-consultations and longer-term effects of CRP-POCT need further evaluation. Overall, the benefits of CRP-POCT outweigh the potential harms (NNTnet = 11).

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