scholarly journals Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

2018 ◽  
Author(s):  
Maria-Dimitra Tsolakidou ◽  
Ioannis A. Stringlis ◽  
Natalia Fanega-Sleziak ◽  
Stella Papageorgiou ◽  
Antria Tsalakou ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Growth Parameters ◽  
Tomato Plants ◽  
Beneficial Effects ◽  
Negative Effect ◽  
Tomato Growth ◽  
Selection Of

AbstractComposts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, forin vitroantifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters inArabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genusBacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth ofArabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect onArabidopsisand tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect onArabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn’t affectArabidopsisgrowth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

scholarly journals Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

2019 ◽  
Author(s):  
Maria-Dimitra Tsolakidou ◽  
Ioannis A Stringlis ◽  
Natalia Fanega-Sleziak ◽  
Stella Papageorgiou ◽  
Antria Tsalakou ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Growth Parameters ◽  
Tomato Plants ◽  
Beneficial Effects ◽  
Negative Effect ◽  
Tomato Growth ◽  
In Vitro Antifungal Activity

Abstract Composts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, for in vitro antifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters in Arabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genus Bacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth of Arabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect on Arabidopsis and tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect on Arabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn't affect Arabidopsis growth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

scholarly journals Trichomes Morphology and Essential Oils Characterization of Field-Growing and In Vitro Propagated Plants of Lavandula pedunculata

2008 ◽  
Vol 14 (S3) ◽  
pp. 148-149 ◽  
Author(s):  
M. Zuzarte ◽  
A.M. Dinis ◽  
C. Cavaleiro ◽  
J. Canhoto ◽  
L. Salgueiro
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oils ◽  
Vegetative Propagation ◽  
Leaf Trichomes ◽  
Economic Exploitation ◽  
Selection Of

The selection of native Lavandula species and their economic exploitation have increased in the last few years. Micropropagation techniques have been used as an alternative for vegetative propagation allowing the multiplication of selected genotypes and chemotypes. Our previous studies showed that the essential oils of Lavandula pedunculata have an important antifungal activity against dermatophyte strains. Therefore, a new line of investigation concerning the in vitro culture of this species is justified. In the present study we compare the morphology of the leaf trichomes and the chemical composition of their essential oils in both field-growing and in vitro propagated plants.

scholarly journals Fenugreek extract application improves plant performance of Alternaria solani infected tomato plants

2021 ◽  
Vol 58 (04) ◽  
pp. 1263-1275
Author(s):  
Rashid Iqbal Khan
Keyword(s):  
Plant Extracts ◽  
Fungal Pathogens ◽  
Early Blight ◽  
Growth Parameters ◽  
Alternaria Solani ◽  
Plant Performance ◽  
Total Phenolic ◽  
Tomato Plants ◽  
Chlorophyll Pigments

Plant extracts (PE’s) has emerged as a safer alternative to manage the fungal pathogens affecting tomato productivity. The current study aimed to evaluate the antimicrobial potential of methanolic fenugreek extract against Alternaria solani, a causal agent of early blight disease in tomato. Fenugreek extract was used at different concentrations of 5%, 10%, 15%, 20% and 25% under in vitro conditions. Results concluded that 25% fenugreek extract significantly reduced the radial growth (2.5 cm) of A. solani under in vitro conditions. Based on in vitro results, three concentrations (5%, 15% and 25%) of fenugreek extract was examined under greenhouse and field conditions. The outcomes expressed that 5% fenugreek extract reduced the disease severity up to 30.19% under greenhouse conditions and up to 40.53% under field trials. Although, application of fenugreek extract had exhibited non-significant impact on vegetative and reproductive growth parameters. However, its application had proved better results as compared to those plants which are infected with A. solani but received zero application of fenugreek extract. Furthermore, the effectiveness of plant extracts was evaluated by variant photosynthetic, antioxidative, polyphenolic and hypersensitive response of A. solani affected tomato plants. The 25% fenugreek extract application had augmented the chlorophyll pigments along with the significant increment of superoxide dismutase (174.16 U mg-1 protein), peroxidase (7.61 µmol min-1 g-1 protein) and catalase activity (4.73 nmol min-1 g-1 protein). Similar outcomes were observed regarding phenolic compounds, where 5% fenugreek extract application had enhanced flavonoid levels (26.62 mg QuE g-1), tannins (1.28 mg TE g-1 extract) and total phenolic contents (2.39 mg GAE g-1) in tomato leaves demonstrating its protective effect against early blight. In dose response, 25% fenugreek extract was most effective in reducing lipid peroxidation and enhancing H2O2 levels. The outcomes of study exhibited the fenugreek extract as an effective strategy to be used against A. solani to control early blight infection in tomato plants. Thus, it can serve as suitable fungicide alternative for resource-poor agriculture areas mainly in developing countries.

scholarly journals Preclinical Testing of Radiopharmaceuticals for the Detection and Characterization of Osteomyelitis: Experiences from a Porcine Model

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4221
Author(s):  
Aage Kristian Olsen Alstrup ◽  
Svend Borup Jensen ◽  
Ole Lerberg Nielsen ◽  
Lars Jødal ◽  
Pia Afzelius
Keyword(s):  
Animal Model ◽  
Animal Models ◽  
Porcine Model ◽  
Animal Experiments ◽  
Radioactive Tracers ◽  
The Individual ◽  
Selection Of

The development of new and better radioactive tracers capable of detecting and characterizing osteomyelitis is an ongoing process, mainly because available tracers lack selectivity towards osteomyelitis. An integrated part of developing new tracers is the performance of in vivo tests using appropriate animal models. The available animal models for osteomyelitis are also far from ideal. Therefore, developing improved animal osteomyelitis models is as important as developing new radioactive tracers. We recently published a review on radioactive tracers. In this review, we only present and discuss osteomyelitis models. Three ethical aspects (3R) are essential when exposing experimental animals to infections. Thus, we should perform experiments in vitro rather than in vivo (Replacement), use as few animals as possible (Reduction), and impose as little pain on the animal as possible (Refinement). The gain for humans should by far exceed the disadvantages for the individual experimental animal. To this end, the translational value of animal experiments is crucial. We therefore need a robust and well-characterized animal model to evaluate new osteomyelitis tracers to be sure that unpredicted variation in the animal model does not lead to a misinterpretation of the tracer behavior. In this review, we focus on how the development of radioactive tracers relies heavily on the selection of a reliable animal model, and we base the discussions on our own experience with a porcine model.

scholarly journals Selection of Trichoderma spp. strains for the control of Sclerotinia sclerotiorum in soybean

2017 ◽  
Vol 52 (12) ◽  
pp. 1140-1148 ◽  
Author(s):  
Patrícia Elias Haddad ◽  
Luis Garrigós Leite ◽  
Cleusa Maria Mantovanello Lucon ◽  
Ricardo Harakava
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Culture Medium ◽  
Harmful Effect ◽  
Soybean Seeds ◽  
Trichoderma Spp ◽  
Negative Effect ◽  
Soybean Plants ◽  
Selection Of

Abstract: The objective of this work was to evaluate, in vitro and in vivo, the potential of Trichoderma spp. strains to control Sclerotinia sclerotiorum in soybeans (Glycine max) and to perform the molecular identification of the best perfoming strains. The effect of 120 strains of Trichoderma spp. on the viability of S. sclerotiorum sclerotia was evaluated in vitro through immersion in suspension of conidia from the antagonists and plating in culture medium. The best performing strains were evaluated in vivo, in a greenhouse, for control of the pathogen inoculated on 'Pintado' soybean seeds and plants. Of the 120 strains tested in vitro, 22 strains of Trichoderma spp. caused 100% inhibition of sclerotia germination. In the greenhouse, five strains inhibited the negative effect of the pathogen on seed germination and two strains increased in up to 67% plant dry matter. The best performing strains were identified as T. koningiopsis (3 strains), T. asperelloides (3), T. atroviride (2), and T. virens (1). Trichoderma strains are able to protect soybean plants from the harmful effect of S. sclerotiorum and, at the same time, they can promote the growth of the aerial part in greenhouse conditions.

scholarly journals In Vitro and In Vivo Characterization of Novel Stable Peptidic Ghrelin Analogs: Beneficial Effects in the Settings of Lipopolysaccharide-Induced Anorexia in Mice

2018 ◽  
Vol 366 (3) ◽  
pp. 422-432 ◽  
Author(s):  
Martina Holubová ◽  
Miroslava Blechová ◽  
Anna Kákonová ◽  
Jaroslav Kuneš ◽  
Blanka Železná ◽  
...  
Keyword(s):  
Beneficial Effects ◽  
In Vivo Characterization

scholarly journals Management of Alternaria leaf blight in tomato plants by mentha essential oil

2020 ◽  
Vol 56 (No. 3) ◽  
pp. 191-196
Author(s):  
Kahkashan Perveen ◽  
Najat A. Bokhari
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Mycelial Growth ◽  
Leaf Blight ◽  
In Vitro Test ◽  
Tomato Plants ◽  
Conidia Germination ◽  
Alternaria Leaf Blight

The essential oil obtained by the hydro-distillation of the leaves of Mentha arvensis Linnaeus was evaluated for its antifungal activity against the causal agent of the Alternaria blight of tomatoes, i.e., Alternaria alternata (Fries) Keissler. The antifungal activity of the mentha essential oil was assessed both in vitro and in vivo. The chemical composition of the mentha oil was also identified by GCMS analysis. The in vitro test revealed that the maximum inhibition in the mycelial growth (93.6%) and conidia germination (90.6%) was at the highest concentration (40 µL/mL), furthermore, it was found that the inhibition of the mycelial growth and conidia germination was dose dependent. The in vivo test proved that the application of the mentha essential oil (40 µL/mL) significantly increased the plant height (84.6%), fresh weight (81.5%) and dry weight (80.0%) when compared to the untreated tomato plants. The disease incidence was 3.5 in the untreated plants, while it was 0.93 for the mentha essential oil treated plants and was 0.08 in the carbendazim treated plants. The GC-MS analysis of the mentha essential oil identified 18 compounds in total, among which the percentage of menthol was the highest (69.2%). The mentha essential oil was successful in managing the Alternaria leaf blight in the tomato plants. Therefore, it can be explored further for the development of a natural fungicide.

scholarly journals Application of Plant Extracts to Control Postharvest Gray Mold and Susceptibility of Apple Fruits to B. cinerea from Different Plant Hosts

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1430 ◽  
Author(s):  
Lina Šernaitė ◽  
Neringa Rasiukevičiūtė ◽  
Alma Valiuškaitė
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Plant Protection ◽  
Gray Mold ◽  
Postharvest Disease ◽  
Cinnamon Extract ◽  
Wide Range ◽  
Plant Hosts ◽  
Apple Fruits

Sustainable plant protection can be applied on apples against fungal pathogens such as Botrytis cinerea (which is responsible for gray mold)—a significant global postharvest disease. This pathogen can affect a wide range of hosts; and fruits may have variable susceptibilities to B. cinerea from different plant hosts. New possibilities to control gray mold in food production are under demand due to the emergence of resistance against antifungal agents in fungal pathogens. Cinnamon, pimento, and laurel extracts were previously assessed for antifungal activities under in vitro conditions and were found to have the potential to be effective against postharvest gray mold. Therefore, this study aimed to investigate the antifungal activity of cinnamon, pimento, and laurel extracts in vitro and against postharvest gray mold on apples to determine the susceptibility of apple fruits to B. cinerea from different plant hosts, and to analyze the chemical composition of the extracts. Apples (cv. “Connell Red”) were treated with different concentrations of extracts and inoculated with B. cinerea isolates from apple and strawberry followed by evaluation of in vitro antifungal activity. The results reveal that most of the concentrations of the extracts that were investigated were not efficient enough when assessed in the postharvest assay, despite having demonstrated a high in vitro antifungal effect. Apples were less susceptible to B. cinerea isolated from strawberry. To conclude, cinnamon extract was found to be the most effective against apple gray mold; however, higher concentrations of the extracts are required for the efficient inhibition of B. cinerea in fruits during storage.

scholarly journals Antifungal Activity of Saponins from the Fruit Pericarp of Sapindus mukorossi against Venturia inaequalis and Botrytis cinerea

Plant Disease ◽  
2018 ◽  
Vol 102 (5) ◽  
pp. 991-1000 ◽  
Author(s):  
Franziska M. Porsche ◽  
Daniel Molitor ◽  
Marco Beyer ◽  
Sophie Charton ◽  
Christelle André ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Antifungal Activity ◽  
Botrytis Cinerea ◽  
Fungal Pathogens ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Venturia Inaequalis ◽  
Field Trials ◽  
Fruit Pericarp

The antifungal activity of an aqueous extract (AE) and the solid fraction of a chloroform-methanol fruit pericarp extract (CME) of Sapindus mukorossi resolved in water was tested for the first time against Venturia inaequalis and Botrytis cinerea—two important fungal pathogens worldwide. In the greenhouse, a CME (1% vol/vol) spray significantly reduced V. inaequalis symptoms and sporulation (99%) on apple seedling leaves (P ≤ 0.05). In field trials, applications of AE (1% vol/vol) reduced the disease severity of B. cinerea on grape, on average, by 63%. Extracts were fractionated by high-performance liquid chromatography and the bioefficacy of the fractions was tested in vitro. Some components of the most fungicidal fraction were identified by liquid chromatography-high resolution mass spectrometry as saponins: sapindoside B (accounting for ≥98% of the total constituents), hederagenin-pentosylhexoside, and oleanolic acid-hexosyl-deoxyhexosyl-hexoside. This fraction inhibited the mycelial growth of V. inaequalis and B. cinerea by 45 and 43%, respectively.

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