scholarly journals Evidence for absence of bilateral transfer of olfactory learned information in Apis dorsata and Apis mellifera

2018 ◽  
Author(s):  
Meenakshi Vijaykumar ◽  
Sandhya Mogily ◽  
Aparna Dutta-Gupta ◽  
Joby Joseph
Keyword(s):  
Apis Mellifera ◽  
Olfactory System ◽  
Contralateral Side ◽  
Apis Dorsata ◽  
Associative Memories ◽  
Olfactory Memory ◽  
Conditioning Paradigm ◽  
Present Evidence ◽  
Summary Statement ◽  
Honeybee Brain

AbstractCapacity and condition under which lateral transfer of olfactory memory is possible in insects are still debated. Here we present evidence consistent with lack of ability to transfer olfactory associative memory in two species of honeybees, Apis mellifera and Apis dorsata in a PER associative conditioning paradigm where the untrained antenna is blocked by an insulating coat. We show that the olfactory system on each side of the bee can learn and retrieve independently and the retrieval using the antenna on the side contralateral to the trained one is not affected by the training. Recreating the paradigm in which the memory on the contralateral side has been reported at three hours after training we see that the memory is available on the contralateral side immediately after training and moreover, training with trained side antenna coated with insulator does not prevent learning, pointing to a possible insufficiency of block of odor stimuli in this paradigm. Bee does not learn the odor stimuli applied to one side alone as a stimulus different from odor presented to both sides. Moreover the behaviour of the bee as a whole can be predicted if the sides are assumed to learn and store independently and the organism as a whole is able to retrieve the memory if either of the sides have learned.Summary StatementThe two halves of honeybee brain store and retrieve olfactory associative memories independently.

scholarly journals Characterization of the olfactory system in Apis dorsata, an Asian honey bee

2018 ◽  
Author(s):  
Sandhya Mogily ◽  
Meenakshi VijayKumar ◽  
Sunil Kumar Sethy ◽  
Joby Joseph
Keyword(s):  
Apis Mellifera ◽  
Learning And Memory ◽  
Honey Bee ◽  
Olfactory System ◽  
Mushroom Body ◽  
Antennal Lobe ◽  
Equivalent Model ◽  
Memory Retention ◽  
Apis Dorsata ◽  
Lateral Protocerebrum

AbstractThe European honeybee, Apis mellifera is the most common insect model system for studying learning and memory. We establish that the olfactory system of Apis dorsata, an Asian species of honeybee as an equivalent model to Apis mellifera to study physiology underlying learning and memory. We created an Atlas of the antennal lobe and counted the number of glomeruli in the antennal lobe of Apis dorsata to be around 165 which is similar to the number in the other honey bee species Apis mellifera and Apis florea. Apis dorsata was found to have five antenno-cerebral tracts namely mACT, lACT and 3 mlACTS which appear identical to Apis mellifera. Intracellular recording showed that the antennal lobe interneurons exhibit temporally patterned odor-cell specific responses. The neuritis of Kenyon cells with cell bodies located in a neighborhood in calyx retain their relative neighborhoods in the peduncle and alpha lobe forming a columnar organization in the mushroom body. Alpha lobe and the calyx of the mushroom body were innervated by extrinsic neurons with cell bodies in the lateral protocerebrum. A set of GABA positive cells in the lateral protocerebrum send their neurites towards alpha-lobe. Apis dorsata was amenable to olfactory conditioning and showed good learning and memory retention at 24 hours. They were amenable to massed and spaced conditioning and could distinguish trained odor from an untrained novel odor.

Differential involvement of glutamate-gated chloride channel splice variants in the olfactory memory processes of the honeybee Apis mellifera

2014 ◽  
Vol 124 ◽  
pp. 137-144 ◽  
Author(s):  
Fabien Démares ◽  
Florian Drouard ◽  
Isabelle Massou ◽  
Cindy Crattelet ◽  
Aurore Lœuillet ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Chloride Channel ◽  
Splice Variants ◽  
Olfactory Memory ◽  
Memory Processes ◽  
Differential Involvement

Effect of a thymol application on olfactory memory and gene expression levels in the brain of the honeybee Apis mellifera

2014 ◽  
Vol 22 (11) ◽  
pp. 8022-8030 ◽  
Author(s):  
Elsa Bonnafé ◽  
Florian Drouard ◽  
Lucie Hotier ◽  
Jean-Luc Carayon ◽  
Pierre Marty ◽  
...  
Keyword(s):  
Gene Expression ◽  
Apis Mellifera ◽  
Olfactory Memory ◽  
Expression Levels ◽  
The Brain ◽  
Gene Expression Levels

Biochemical properties of heterologously expressed and native adenylyl cyclases from the honeybee brain (Apis mellifera L.)

2010 ◽  
Vol 40 (8) ◽  
pp. 573-580 ◽  
Author(s):  
Nadine Fuss ◽  
Samir Mujagic ◽  
Joachim Erber ◽  
Sebastian Wachten ◽  
Arnd Baumann
Keyword(s):  
Apis Mellifera ◽  
Biochemical Properties ◽  
Adenylyl Cyclases ◽  
Honeybee Brain

scholarly journals Honeybees ( Apis mellifera ) learn to discriminate the smell of organic compounds from their respective deuterated isotopomers

2014 ◽  
Vol 281 (1778) ◽  
pp. 20133089 ◽  
Author(s):  
Wulfila Gronenberg ◽  
Ajay Raikhelkar ◽  
Eric Abshire ◽  
Jennifer Stevens ◽  
Eric Epstein ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Receptor Protein ◽  
Sensory Cells ◽  
Molecular Vibrations ◽  
Conditioning Paradigm ◽  
Odour Discrimination ◽  
Odorant Molecule ◽  
Proboscis Extension ◽  
Structural Aspects ◽  
Primary Interaction

The understanding of physiological and molecular processes underlying the sense of smell has made considerable progress during the past three decades, revealing the cascade of molecular steps that lead to the activation of olfactory receptor (OR) neurons. However, the mode of primary interaction of odorant molecules with the OR proteins within the sensory cells is still enigmatic. Two different concepts try to explain these interactions: the ‘odotope hypothesis’ suggests that OR proteins recognize structural aspects of the odorant molecule, whereas the ‘vibration hypothesis’ proposes that intra-molecular vibrations are the basis for the recognition of the odorant by the receptor protein. The vibration hypothesis predicts that OR proteins should be able to discriminate compounds containing deuterium from their common counterparts which contain hydrogen instead of deuterium. This study tests this prediction in honeybees ( Apis mellifera ) using the proboscis extension reflex learning in a differential conditioning paradigm. Rewarding one odour (e.g. a deuterated compound) with sucrose and not rewarding the respective analogue (e.g. hydrogen-based odorant) shows that honeybees readily learn to discriminate hydrogen-based odorants from their deuterated counterparts and supports the idea that intra-molecular vibrations may contribute to odour discrimination.

scholarly journals Olfactory coding in honeybees

2021 ◽  
Vol 383 (1) ◽  
pp. 35-58 ◽  
Author(s):  
Marco Paoli ◽  
Giovanni C. Galizia
Keyword(s):  
Apis Mellifera ◽  
Molecular Mechanisms ◽  
Memory Formation ◽  
Behavioral Data ◽  
Olfactory Perception ◽  
Research Model ◽  
Ideal Model ◽  
Olfactory Memory ◽  
Olfactory Coding ◽  
Genetic Tools

Abstract With less than a million neurons, the western honeybee Apis mellifera is capable of complex olfactory behaviors and provides an ideal model for investigating the neurophysiology of the olfactory circuit and the basis of olfactory perception and learning. Here, we review the most fundamental aspects of honeybee’s olfaction: first, we discuss which odorants dominate its environment, and how bees use them to communicate and regulate colony homeostasis; then, we describe the neuroanatomy and the neurophysiology of the olfactory circuit; finally, we explore the cellular and molecular mechanisms leading to olfactory memory formation. The vastity of histological, neurophysiological, and behavioral data collected during the last century, together with new technological advancements, including genetic tools, confirm the honeybee as an attractive research model for understanding olfactory coding and learning.

scholarly journals Klasifikasi Madu Berdasarkan Jenis Lebah (Apis dorsata versus Apis mellifera) Menggunakan Spektroskopi Ultraviolet dan Kemometrika

2020 ◽  
Vol 25 (4) ◽  
pp. 564-573
Author(s):  
Diding Suhandy ◽  
Meinilwita Yulia ◽  
Kusumiyati Kusumiyati
Keyword(s):  
Apis Mellifera ◽  
Phenolic Compounds ◽  
Spectral Data ◽  
Uv Spectroscopy ◽  
Low Cost ◽  
Moving Average ◽  
Apis Dorsata ◽  
Classification Rate ◽  
Standard Normal Variate ◽  
Standard Normal

In this research, spectral data in UV region (200-400 nm) alongside PCA and SIMCA chemometrics were used to classify two types of honey obtained from different honeybees (Apis dorsata versus Apis mellifera). A total of 200 Durian monofloral honey samples from Apis dorsata and 120 samples for Longan monofloral honey from Apis mellifera were prepared. Therefore, spectral data were recorded based on the following parameters: range of acquisition 200-400 nm, transmittance mode, and interval 1 nm. In addition, the original spectra were transformed using three different algorithms: moving average smoothing with 11 segments, standard normal variate (SNV), and Savitzky-Golay 1st derivative with 11 segments and 2 ordos. The result of PCA using transformed spectra in the range of 250-400 nm explained the possibility of clearly separating Durian and Longan honey along the PC1 axis, with 98% variance, while the SIMCA showed a 100% proper classification rate for all prediction samples. In addition, several important wavelengths were identified alongside high x-loadings values at 270 and 300 nm. These results were closely related to the absorbance of important phenolic compounds in honey, including benzoic, salicylic, and aryl-alyphatic acids. The results demonstrate a probability to establish simple and low-cost honey authentication systems, using UV spectroscopy and chemometrics on free-chemical in sample preparations. Keywords: authentication, Apis dorsata, Apis mellifera, SIMCA, UV spectroscopy

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