scholarly journals Charting the emergent organotypic landscape of the mammalian gut endoderm at single-cell resolution

2018 ◽  
Author(s):  
Sonja Nowotschin ◽  
Manu Setty ◽  
Ying-Yi Kuo ◽  
Vincent Lui ◽  
Vidur Garg ◽  
...  
Keyword(s):  
Developmental Trajectories ◽  
Organ System ◽  
Mammalian Development ◽  
Spatial Convergence ◽  
Organ Specific ◽  
Embryonic Origin ◽  
Spatio Temporal ◽  
Gut Endoderm ◽  
Anterior Posterior

AbstractTo comprehensively delineate the ontogeny of an organ system, we generated 112,217 singlecell transcriptomes representing all endoderm populations within the mouse embryo until midgestation. We employed graph-based approaches to model differentiating cells for spatio-temporal characterization of developmental trajectories. Our analysis reveals the detailed architecture of the emergence of the first (primitive or extra-embryonic) endodermal population and pluripotent epiblast. We uncover an unappreciated relationship between descendants of these lineages, before the onset of gastrulation, suggesting that mixing of extra-embryonic and embryonic endoderm cells occurs more than once during mammalian development. We map the trajectories of endoderm cells as they acquire embryonic versus extra-embryonic fates, and their spatial convergence within the gut endoderm; revealing them to be globally similar but retaining aspects of their lineage history. We observe the regionalized localization of cells along the forming gut tube, reflecting their extra-embryonic or embryonic origin, and their coordinate patterning into organ-specific territories along the anterior-posterior axis.

scholarly journals Fluorescence Fluctuation Spectroscopy enables quantification of potassium channel subunit dynamics and stoichiometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Giulia Tedeschi ◽  
Lorenzo Scipioni ◽  
Maria Papanikolaou ◽  
Geoffrey W. Abbott ◽  
Michelle A. Digman
Keyword(s):  
Plasma Membrane ◽  
Protein Diffusion ◽  
Ion Diffusion ◽  
Fluorescence Fluctuation Spectroscopy ◽  
Biophysical Characterization ◽  
Kv Channels ◽  
Subunit Stoichiometry ◽  
Spatio Temporal ◽  
Voltage Sensing Domain

AbstractVoltage-gated potassium (Kv) channels are a family of membrane proteins that facilitate K+ ion diffusion across the plasma membrane, regulating both resting and action potentials. Kv channels comprise four pore-forming α subunits, each with a voltage sensing domain, and they are regulated by interaction with β subunits such as those belonging to the KCNE family. Here we conducted a comprehensive biophysical characterization of stoichiometry and protein diffusion across the plasma membrane of the epithelial KCNQ1-KCNE2 complex, combining total internal reflection fluorescence (TIRF) microscopy and a series of complementary Fluorescence Fluctuation Spectroscopy (FFS) techniques. Using this approach, we found that KCNQ1-KCNE2 has a predominant 4:4 stoichiometry, while non-bound KCNE2 subunits are mostly present as dimers in the plasma membrane. At the same time, we identified unique spatio-temporal diffusion modalities and nano-environment organization for each channel subunit. These findings improve our understanding of KCNQ1-KCNE2 channel function and suggest strategies for elucidating the subunit stoichiometry and forces directing localization and diffusion of ion channel complexes in general.

SPATIO-TEMPORAL CHARACTERIZATION of litter at a touristic sandy beach in south Brazil

2021 ◽  
pp. 116927
Author(s):  
Bruna de Ramos ◽  
Melanie Vianna Alencar ◽  
Fábio Lameiro Rodrigues ◽  
Ana Luzia de Figueiredo Lacerda ◽  
Maíra Carneiro Proietti
Keyword(s):  
Sandy Beach ◽  
Spatio Temporal ◽  
South Brazil

scholarly journals Spatio-temporal characterization of landscape fire in relation to anthropogenic activity and climatic variability over the Western Himalaya, India

2021 ◽  
Vol 58 (2) ◽  
pp. 281-299
Author(s):  
Somnath Bar ◽  
Bikash Ranjan Parida ◽  
Gareth Roberts ◽  
Arvind Chandra Pandey ◽  
Prasenjit Acharya ◽  
...  
Keyword(s):  
Climatic Variability ◽  
Western Himalaya ◽  
Anthropogenic Activity ◽  
Spatio Temporal

Characterization of a gadolinium-labeled cholesterol derivative as an organ-specific contrast agent for adrenal MR imaging

1995 ◽  
Vol 5 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Andreas Muhler ◽  
Johannes Platzek ◽  
Bernd Radüchel ◽  
Thomas Frenzel ◽  
Hanns-Joachim Weinmann
Keyword(s):  
Mr Imaging ◽  
Contrast Agent ◽  
Organ Specific ◽  
Cholesterol Derivative ◽  
Adrenal Mr

Damage Detection in Flexible Propeller Beam Structures by Exploiting Impact-Induced Coupled Acceleration Signals

2011 ◽  
Author(s):  
Ioannis T. Georgiou
Keyword(s):  
Data Driven ◽  
Distributed Information ◽  
Modal Expansion ◽  
Shape Difference ◽  
Spatio Temporal ◽  
Temporal Sample ◽  
High Level ◽  
Proper Orthogonal ◽  
Acceleration Signals

A local damage at the tip of a composite propeller is diagnosed by properly comparing its impact-induced free coupled dynamics to that of a pristine wooden propeller of the same size and shape. This is accomplished by creating indirectly via collocated measurements distributed information for the coupled acceleration field of the propellers. The powerful data-driven modal expansion analysis delivered by the Proper Orthogonal Decomposition (POD) Transform reveals that ensembles of impact-induced collocated coupled experimental acceleration signals are underlined by a high level of spatio-temporal coherence. Thus they furnish a valuable spatio-temporal sample of coupled response induced by a point impulse. In view of this fact, a tri-axial sensor was placed on the propeller hub to collect collocated coupled acceleration signals induced via modal hammer nondestructive impacts and thus obtained a reduced order characterization of the coupled free dynamics. This experimental data-driven analysis reveals that the in-plane unit components of the POD modes for both propellers have similar shapes-nearly identical. For the damaged propeller this POD shape-difference is quite pronounced. The shapes of the POD modes are used to compute indices of difference reflecting directly damage. At the first POD energy level, the shape-difference indices of the damaged composite propeller are quite larger than those of the pristine wooden propeller.

scholarly journals Characterization of spatio-temporal epidural event-related potentials for mouse models of psychiatric disorders

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Xin Wang ◽  
António Pinto-Duarte ◽  
M. Margarita Behrens ◽  
Xianjin Zhou ◽  
Terrence J. Sejnowski
Keyword(s):  
Psychiatric Disorders ◽  
Mouse Models ◽  
Event Related Potentials ◽  
Related Potentials ◽  
Spatio Temporal
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