scholarly journals A Novel Root-Knot Nematode Resistance QTL on Chromosome Vu01 in Cowpea

2018 ◽  
Author(s):  
Arsenio D. Ndeve ◽  
Jansen R. P. Santos ◽  
William. C. Matthews ◽  
Bao L. Huynh ◽  
Yi-Ning Guo ◽  
...  
Keyword(s):  
Genetic Resistance ◽  
Nematode Resistance ◽  
Egg Mass ◽  
Resistance Locus ◽  
Root Knot Nematode ◽  
Resistance Qtl ◽  
Cowpea Cultivars ◽  
Degree Of Dominance ◽  
Allelism Tests ◽  
Production Areas

ABSTRACTThe root-knot nematode (RKN) species Meloidogyne incognita and M. javanica cause substantial root system damage and suppress yield of susceptible cowpea cultivars. The narrow-based genetic resistance conferred by the Rk gene, present in some commercial cultivars, is not effective against Rk-virulent populations found in several cowpea production areas. The dynamics of virulence within RKN populations require a broadening of the genetic base of resistance in elite cowpea cultivars. As part of this goal, F1 and F2 populations from the cross CB46-Null (susceptible) x FN-2-9-04 (resistant) were phenotyped for M. javanica induced root-galling (RG) and egg-mass production (EM) in controlled growth chamber and greenhouse infection assays. In addition, F2:3 families of the same cross were phenotyped for RG on field sites infested with Rk-avirulent M. incognita and M. javanica. The response of F1 to RG and EM indicated that resistance to RKN in FN-2-9-04 is partially dominant, as supported by the degree of dominance in the F2 and F2:3 populations. Two QTLs associated with both RG and EM resistance were detected on chromosomes Vu01 and Vu04. The QTL on Vu01 was most effective against aggressive M. javanica, whereas both QTLs were effective against avirulent M. incognita. Allelism tests with CB46 x FN-2-9-04 progeny indicated that these parents share the same RKN resistance locus on Vu04, but the strong, broad-based resistance in FN-2-9-04 is conferred by the additive effect of the novel resistance QTL on Vu01. This novel resistance in FN-2-9-04 is an important resource for broadening RKN resistance in elite cowpea cultivars.

Genetic mapping of a root-knot nematode resistance locus in Trifolium

Euphytica ◽  
2005 ◽  
Vol 143 (1-2) ◽  
pp. 85-92 ◽  
Author(s):  
Brent Barrett ◽  
Chris Mercer ◽  
Derek Woodfield
Keyword(s):  
Genetic Mapping ◽  
Nematode Resistance ◽  
Resistance Locus ◽  
Root Knot Nematode

Identification and Evaluation of Additional Sources of Resistance to Peanut Root-knot Nematode in Arachis hypogaea L.1

1996 ◽  
Vol 23 (2) ◽  
pp. 91-94 ◽  
Author(s):  
C. Corley Holbrook ◽  
James P. Noe ◽  
Michael G. Stephenson ◽  
William F. Anderson
Keyword(s):  
Arachis Hypogaea ◽  
Field Experiments ◽  
Germplasm Collection ◽  
Economic Losses ◽  
Egg Mass ◽  
Root Knot Nematode ◽  
Sources Of Resistance ◽  
Nematode Reproduction ◽  
Plant Introductions ◽  
Production Areas

Abstract The root-knot nematode (Meloidogyne arenaria race 1) causes significant economic losses throughout the peanut (Arachis hypogaea) production areas of the southern U.S. Chemicals for control of this pest are becoming increasingly limited, and there are no peanut cultivars with resistance. Seven moderately resistant plant introductions have been identified; however, less than 25% of the germplasm collection has been examined for resistance based on nematode reproduction. The objectives of this work were to examine an additional 1000 plant introductions for resistance to the peanut root-knot nematode and to compare the most resistant introductions to previously reported sources of resistance. Preliminary greenhouse screening trials were conducted to rate severity of root galling and amount of egg mass production. Seventeen accessions were selected based on a mean egg mass rating of less than or equal to three. These selections were reevaluated in additional greenhouse and field experiments to quantify levels of resistance and to directly compare these sources of resistance to those previously reported. Eight accessions had a significantly higher level of resistance (lower egg mass rating) than Florunner; however, none had a significantly higher level of resistance than those previously reported. Results of this study identified additional sources of resistance which may provide unique genes for resistance. In addition, two of these new sources of resistance (PI 298848 and PI 311265) exhibited significantly higher yield than those previously identified when grown in soil heavily infested with M. arenaria.

scholarly journals Root-knot Nematode Resistance in Cucumis Species

HortScience ◽  
1997 ◽  
Vol 32 (5) ◽  
pp. 880-881 ◽  
Author(s):  
Perry E. Nugent ◽  
P.D. Dukes
Keyword(s):  
Cucumis Melo ◽  
Gulf Coast ◽  
Nematode Resistance ◽  
The United States ◽  
Economic Losses ◽  
Egg Mass ◽  
Root Knot Nematode ◽  
Inoculum Level ◽  
Resistant Species ◽  
Nematode Eggs

The southern root-knot nematode, Meloidogyne incognita [(Kofoid & White) Chitwood], causes serious economic losses to melon (Cucumis melo L.) production in the United States. The present study was conducted to determine if separable differences in nematode resistance of Cucumis melo could be found at some inoculum level. Five C. melo lines were compared with Cucumis metuliferus Naud. (C701A), a highly resistant species, for root necrosis, galling, egg mass production, and reproduction when inoculated at 0, 500, 1000, 2000, or 5000 nematode eggs per plant. Using these criteria, melon line C880 inoculated with 1000 eggs per plant was highly susceptible, while PI140471, PI 183311, and the cultivars Chilton, Georgia 47, Gulf Coast, Planters Jumbo, and Southland were less susceptible. In greenhouse tests with an inoculum level of 1000 eggs per plant, low levels of resistance were evident. A thorough screening of the available germplasm against M. incognita may identify higher levels of root-knot nematode resistance for incorporation into improved melon cultivars.

scholarly journals Characterization of Resistance Conferred by the N gene to Meloidogyne arenaria Races 1 and 2, M. hapla, and M. javanica in Two Sets of Isogenic Lines of Capsicum annuum L.

2000 ◽  
Vol 125 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Judy A. Thies ◽  
Richard L. Fery
Keyword(s):  
Capsicum Annuum ◽  
Nematode Resistance ◽  
N Gene ◽  
Recurrent Parent ◽  
Resistance Locus ◽  
Meloidogyne Arenaria ◽  
Meloidogyne Hapla ◽  
Root Knot Nematode ◽  
Race 1 ◽  
Race 2

Two isogenic sets of bell pepper (Capsicum annuum L.) lines (differing at the N root-knot nematode resistance locus) were characterized for resistance to Meloidogyne arenaria (Neal) Chitwood races 1 and 2, M. hapla Chitwood, and M. javanica (Treub) Chitwood in greenhouse and growth chamber tests. The isogenic sets of C. annuum were `Charleston Belle' (NN) and `Keystone Resistant Giant' (nn-recurrent parent), and `Carolina Wonder' (NN) and `Yolo Wonder B' (nn-recurrent parent). Meloidogyne arenaria race 1 is pathogenic to C. annuum. `Charleston Belle' and `Carolina Wonder' exhibited high resistance to M. arenaria race 1. Their respective recurrent backcross parents, `Keystone Resistant Giant' and `Yolo Wonder B', were susceptible to M. arenaria. Meloidogyne arenaria race 2 and M. javanica are not highly pathogenic to pepper. However, `Charleston Belle' and `Carolina Wonder' both exhibited higher (P≤0.05) resistance to M. arenaria race 2 and M. javanica than `Keystone Resistant Giant' and `Yolo Wonder B'. Meloidogyne hapla is pathogenic to pepper. Both `Charleston Belle' and `Carolina Wonder' and their respective recurrent parents, `Keystone Resistant Giant' and `Yolo Wonder B', were susceptible to M. hapla. We concluded that the N gene confers resistance to M. arenaria races 1 and 2, and M. javanica in C. annuum, but the N gene does not condition resistance to M. hapla.

scholarly journals Characterization of New Sources of Resistance in Cowpea to the Southern Root-knot Nematode

HortScience ◽  
1994 ◽  
Vol 29 (6) ◽  
pp. 678-679 ◽  
Author(s):  
Richard L. Fery ◽  
Philip D. Dukes ◽  
Judy A. Thies
Keyword(s):  
Egg Production ◽  
Nematode Resistance ◽  
Field Studies ◽  
Egg Mass ◽  
Root Knot Nematode ◽  
Sources Of Resistance ◽  
Dominant Type ◽  
Plant Introductions ◽  
Nematode Resistance Gene ◽  
Southern Root Knot Nematode

A series of greenhouse and field studies was conducted over 9 years to characterize three new sources of resistance in cowpea [Vigna unguiculata (L.) Walp.] to the southern root-knot nematode [Meloidogyne incognita (Kofoid & White) Chitwood] and to determine if the resistances are conditioned by genes allelic to the Rk root-knot nematode resistance gene in `Mississippi Silver'. Three plant introductions (PI), PI 441917, PI 441920, and PI 468104, were evaluated for reaction to M. incognita in four greenhouse tests, and in every test each PI exhibited less galling, egg mass formation, or egg production than `Mississippi Silver'. F2 populations of the crosses between `Mississippi Silver' and each of the three resistant PIs were also evaluated for root-knot nematode resistance in a greenhouse test. None of the F2 populations segregated for resistance, indicating that PI 441917, PI 441920, and PI 468104 each has a gene conditioning resistance that is allelic to the Rk gene in `Mississippi Silver'. Our observations on the superior levels of resistances exhibited by PI 441917, PI 441920, and PI 468104 suggest that the allele at the Rk locus in these lines may not be the Rk allele, but one or more alleles that condition a superior, dominant-type resistance. The availability of additional dominant alleles would broaden the genetic base for root-knot nematode resistance in cowpea.

scholarly journals A Technique for Screening Grape Germplasm for Resistance to Meloidogyne incognita

Plant Disease ◽  
2001 ◽  
Vol 85 (10) ◽  
pp. 1052-1054 ◽  
Author(s):  
P. M. Cousins ◽  
M. A. Walker
Keyword(s):  
Vitis Vinifera ◽  
Meloidogyne Incognita ◽  
Nematode Resistance ◽  
Egg Mass ◽  
Root Knot Nematode ◽  
Egg Masses ◽  
Nematode Eggs ◽  
Highly Correlated

A technique to evaluate the root-knot nematode resistance of grape seedlings was developed. Seedlings of rootstock crosses and nematode-susceptible Vitis vinifera cvs. Colombard and Carignane were inoculated with Meloidogyne incognita juveniles. Reproduction of nematodes on individual plants was measured by counting the number of egg masses stained with eosin and the number of eggs present. Egg mass counts were highly correlated with egg counts. Resistant and susceptible cultivars could be clearly distinguished by the number of egg masses produced on vegetatively propagated cuttings. It is concluded that egg mass counting can substitute for the more laborious and time-consuming methods of counting nematode eggs or juveniles in the evaluation of root-knot nematode resistance in Vitis.

Genetic and physical localization of the root-knot nematode resistance locus Mi in tomato

1998 ◽  
Vol 257 (3) ◽  
pp. 376-385 ◽  
Author(s):  
I. Kaloshian ◽  
J. Yaghoobi ◽  
T. Liharska ◽  
J. Hontelez ◽  
D. Hanson ◽  
...  
Keyword(s):  
Nematode Resistance ◽  
Resistance Locus ◽  
Root Knot Nematode

scholarly journals QTL-seq reveals a major root-knot nematode resistance locus on chromosome 11 in rice (Oryza sativa L.)

Euphytica ◽  
2019 ◽  
Vol 215 (7) ◽  
Author(s):  
Zobaida Lahari ◽  
Antonio Ribeiro ◽  
Partha Talukdar ◽  
Brennan Martin ◽  
Zeynab Heidari ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Nematode Resistance ◽  
Resistance Locus ◽  
Chromosome 11 ◽  
Root Knot Nematode

A Southern Root-Knot Nematode Resistance QTL Linked to the T -Locus in Soybean

Crop Science ◽  
2009 ◽  
Vol 49 (2) ◽  
pp. 467-472 ◽  
Author(s):  
Zachary P. Shearin ◽  
Steve L. Finnerty ◽  
E. Dale Wood ◽  
Richard S. Hussey ◽  
H. Roger Boerma
Keyword(s):  
Nematode Resistance ◽  
Root Knot Nematode ◽  
Resistance Qtl ◽  
Southern Root Knot Nematode ◽  
T Locus
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