scholarly journals Topical cream with live lactobacilli modulates the skin microbiome and reduce acne symptoms

2018 ◽  
Author(s):  
Sarah Lebeer ◽  
Eline Oerlemans ◽  
Ingmar Claes ◽  
Sander Wuyts ◽  
Tim Henkens ◽  
...  
Keyword(s):  
Therapeutic Option ◽  
Antibiotic Use ◽  
Skin Microbiome ◽  
Body Site ◽  
Skin Microbiota ◽  
Inflammatory Lesions ◽  
Commensal Microbiota ◽  
Topical Cream ◽  
Oil In Water ◽  
Human Vagina

SummaryThe skin is home to an important part of our commensal microbiota, despite it being a cool, acidic and desiccated environment. Tailored microbiome modulation approaches with, for example probiotics, are highly challenging for this body site. Here we show by next-generating sequencing that Lactobacillus taxa -especially those known to be dominant in the human vagina- are underestimated members of the skin microbiota. Specific Lactobacillus strains were selected in the lab and formulated in a viable form in an oil in water-based topical cream. Facial application by patients with mild-to-moderate acne symptoms was able to reduce inflammatory lesions and comedone formation. This was associated with a temporary modulation of the skin microbiome, including a reduction in relative abundance of staphylococci and an increase in lactobacilli. Skin microbiome modulation by addition of carefully formulated lactobacilli seems to be new therapeutic option to reduce antibiotic use for common acne symptoms.

scholarly journals Structural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from India

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nitin Bayal ◽  
Sunil Nagpal ◽  
Mohammed Monzoorul Haque ◽  
Milind S. Patole ◽  
Yogesh Shouche ◽  
...  
Keyword(s):  
Denaturing Gradient Gel Electrophoresis ◽  
Therapeutic Option ◽  
Normal Skin ◽  
Skin Microbiome ◽  
Skin Microbiota ◽  
Nested Polymerase Chain Reaction ◽  
Lesional Skin ◽  
Leprosy Patients ◽  
Gradient Gel Electrophoresis

AbstractAlthough skin is the primary affected organ in Leprosy, the role of the skin microbiome in its pathogenesis is not well understood. Recent reports have shown that skin of leprosy patients (LP) harbours perturbed microbiota which grants inflammation and disease progression. Herein, we present the results of nested Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) which was initially performed for investigating the diversity of bacterial communities from lesional skin (LS) and non-lesional skin (NLS) sites of LP (n = 11). Further, we performed comprehensive analysis of 16S rRNA profiles corresponding to skin samples from participants (n = 90) located in two geographical locations i.e. Hyderabad and Miraj in India. The genus Staphylococcus was observed to be one of the representative bacteria characterizing healthy controls (HC; n = 30), which in contrast was underrepresented in skin microbiota of LP. Taxa affiliated to phyla Firmicutes and Proteobacteria were found to be signatures of HC and LS, respectively. Observed diversity level changes, shifts in core microbiota, and community network structure support the evident dysbiosis in normal skin microbiota due to leprosy. Insights obtained indicate the need for exploring skin microbiota modulation as a potential therapeutic option for leprosy.

scholarly journals Microbiome and Metabolome Analyses Reveal Novel Interplay Between the Skin Microbiota and Plasma Metabolites in Psoriasis

2021 ◽  
Vol 12 ◽  
Author(s):  
Dongmei Chen ◽  
Jingquan He ◽  
Jinping Li ◽  
Qian Zou ◽  
Jiawei Si ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Metabolic Pathways ◽  
Pathogenic Bacteria ◽  
Plasma Metabolites ◽  
Skin Microbiome ◽  
Inflammatory Skin Disease ◽  
Skin Microbiota ◽  
Plasma Metabolome ◽  
Pathological Mechanism ◽  
New Evidence

Psoriasis is a chronic inflammatory skin disease that affects millions of people worldwide. There is still no effective approach for the clinical treatment of psoriasis. This is largely due to the lack of understanding of the pathological mechanism. Here, we comprehensively characterized the skin microbiome and plasma metabolome alterations of psoriasis patients. We observed that some pathogenic bacteria, including Vibrio, were significantly increased in psoriasis patients. The metabolomics results showed alterations in some metabolic pathways, especially pathways for lipid metabolism. In addition, microbiome-specific metabolites, including bile acids and kynurenine, were significantly changed. Correlation analysis revealed the interplay between the skin microbiota and plasma metabolites, especially between Vibrio and several lipids. Our results provide new evidence for the interplay between the skin microbiome and plasma metabolites, which is dramatically disrupted in psoriasis patients. This study also revealed the mechanism underlying the pathogenesis of psoriasis.

scholarly journals Efficacy of Phototherapy With 308-nm Excimer Light for Skin Microbiome Dysbiosis and Skin Barrier Dysfunction in Canine Atopic Dermatitis

2021 ◽  
Vol 8 ◽  
Author(s):  
Ju-Yong Park ◽  
Seon-Myeong Kim ◽  
Jung-Hyun Kim
Keyword(s):  
Atopic Dermatitis ◽  
Relative Abundance ◽  
Barrier Function ◽  
Therapeutic Option ◽  
Clinical Signs ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Skin Microbiome ◽  
Canine Atopic Dermatitis ◽  
Allergic Skin

The management of canine atopic dermatitis, an allergic skin disorder, is challenging. To investigate the effect of phototherapy using a 308-nm excimer light as a topical treatment for canine atopic dermatitis, 10 dogs with canine atopic dermatitis and 10 with non-allergic skin were enrolled in this study. Phototherapy was applied every 7 days for a total of 2 months. The skin microbiome, skin barrier function, and clinical outcomes were evaluated after phototherapy. Phototherapy significantly changed the composition of the skin microbiome of dogs with atopic dermatitis and significantly increased the relative abundance of the phyla Actinobacteria and Cyanobacteria. It significantly alleviated the clinical signs of canine atopic dermatitis without serious adverse effects. Transepidermal water loss, as a measure of skin barrier function, significantly decreased after phototherapy. In addition, phototherapy increased microbial diversity and decreased the relative abundance of Staphylococcus pseudintermedius associated with the severity of canine atopic dermatitis. These results suggest that the excimer light therapy is a suitable and safe therapeutic option for canine atopic dermatitis, which is also a spontaneous animal model of atopic dermatitis.

scholarly journals Rhodomyrtus tomentosa Fruit Extract and Skin Microbiota: A Focus on C. acnes Phylotypes in Acne Subjects

Cosmetics ◽  
2020 ◽  
Vol 7 (3) ◽  
pp. 53 ◽  
Author(s):  
Sandie Gervason ◽  
Isabelle Metton ◽  
Elodie Gemrot ◽  
Edwige Ranouille ◽  
Gilbert Skorski ◽  
...  
Keyword(s):  
Active Ingredient ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Skin Microbiome ◽  
Skin Microbiota ◽  
Cutibacterium Acnes ◽  
Culture Independent ◽  
Rrna Gene Sequencing ◽  
Abundant Genus ◽  
Rhodomyrtus Tomentosa

Knowing that Rhodomyrtus tomentosa is known to have antibacterial effects, this study investigated the skin microbiota with a focus on Cutibacterium acnes (C. acnes) phylotypes in subjects with acne, and determined microbiota changes after 28 days of treatment with berries Rhodomyrtus tomentosa as an active ingredient (RT). Skin swabs from seventeen acne subjects were collected and the skin microbiome was analyzed using 16S rRNA gene sequencing. A culture-independent next-generation sequencing (NGS)-based SLST (single-locus sequence typing) approach was aimed at evaluating RT extract effects on C. acnes phylotype repartition. Clinical evaluations (lesion counts) were performed at baseline (D0) and after 28 days (D28) of twice-daily application of the RT active ingredient. We determined: (1) the skin microbiota at D0 was dominated by Actinobacteria followed by Firmicutes and Proteobacteria; (2) at the genus level, Cutibacterium was the most abundant genus followed by Staphylococcus and Corynebacterium; (3) C. acnes was the major species in terms of mean abundance, followed by Staphylococcus epidermidis (S. epidermidis) and Staphylococcus hominis (S. hominis); and (4) phylotype IA1 was most represented, with a predominance of SLST type A1, followed by phylotypes II, IB, IA2, IC, and III. After 28 days of RT extract treatment, phylotype repartition were modified with a decrease in abundance (approximately 4%) of phylotype IA1 and an increase in phylotype II and III. Cutibacterium granulosum (C. granulosum) abundance also decreased. Reduction of retentional and inflammatory lesions was also noted only after RT treatment; thus, RT extract acts as a microbiota-regulating agent.

scholarly journals 897. Prevalence of Candida auris at Body Sites, Characterization of Skin Microbiota, and Relation of Chlorhexidine Gluconate (CHG) Skin Concentration to C. auris Detection Among Patients at a High-Prevalence Ventilator-Capable Skilled Nursing Facility (vSNF) with Established CHG Bathing

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S25-S26 ◽  
Author(s):  
Mary K Hayden ◽  
Thelma E Dangana ◽  
Rachel D Yelin ◽  
Michael Schoeny ◽  
Pamela B Bell ◽  
...  
Keyword(s):  
Skilled Nursing Facility ◽  
Skin Microbiome ◽  
Body Site ◽  
Candida Auris ◽  
Candida Spp ◽  
Nursing Facility ◽  
Skilled Nursing ◽  
Mechanically Ventilated ◽  
High Prevalence ◽  
High Concentrations

Abstract Background vSNF patients are at high risk of colonization and infection with C. auris. CHG bathing has been used as an intervention to reduce nosocomial transmission of multi-drug-resistant organisms, but its effect on C. auris is unclear. Methods We studied a 70-bed ventilator ward in a 300-bed vSNF in Chicago, IL with a high prevalence of C. auris and established CHG bathing. Swab samples were collected from patients for culture, microbiome analysis, and CHG skin concentration testing (Table 1). Results We collected 2,467 samples (950 culture, 950 microbiome, 567 CHG) from 57 patients during 2 surveys conducted January–March 2019. Forty-six (81%) patients had C. auris cultured from ≥1 body site. Mean (±SD) age was 59 (±14) years, 40% were women, 70% were African American, mean (±SD) Charlson score was 3 (±2). Patients colonized with C. auris were more likely to be mechanically ventilated (50% vs. 0%, P < 0.001), have a gastrostomy tube (78% vs. 27%, P < 0.001) or have urinary catheter (72% vs. 23%, P = 0.01) than noncolonized patients. Frequency of C. auris isolation varied among 10 body sites tested (P < 0.001); colonization of anterior nares (41%) and hands (40%) was detected most often (Figure 1). By ITS1 analysis, all isolates were members of the C. auris South American clade. Skin microbiome sequencing confirmed culture Results. While Malassezia is the dominant genera observed in healthy volunteers and patients in this vSNF, C. auris was observed to dominate the fungal community of multiple skin sites, including nares, hands, inguinal, toe web (Figure 2). Other Candida spp. were also identified on the skin of patients in the current study, but at lower relative abundance. CHG was detected on skin of 52 (91%) patients (median CHG concentration 19.5 µg/mL; IQR 4.9–78.1 µg/mL). In a mixed-effects model controlling for body site and multiple measurements per patient, odds of C. auris detection by culture were less at CHG concentrations ≥625 µg/mL than at lower concentrations (Figure 3; OR 0.25, 95% CI: 0.10–0.66; P = 0.005). Conclusion Frequent C. auris colonization of vSNF patients’ anterior nares and hands suggests that nasal decolonization and patient hand hygiene are potential options to reduce C. auris transmission. High concentrations of CHG may be needed to suppress C. auris on skin. Disclosures All Authors: No reported Disclosures.

scholarly journals Profiling Skin Microbiome in Healthy Young Adult Representing Javanese, Papuans, and Chinese Descent in Indonesia

2021 ◽  
Vol 28 (4) ◽  
pp. 249-261
Author(s):  
Stella Vania ◽  
Amarila Malik
Keyword(s):  
Skin Diseases ◽  
Geographical Location ◽  
Alpha Diversity ◽  
Ethnic Origin ◽  
Skin Microbiome ◽  
Microbiota Composition ◽  
Skin Microbiota ◽  
Pathogen Invasion ◽  
Chinese Descent ◽  
Microbiome Research

Skin serves as the first physical barrier and biological barrier by the colonization of commensal bacteria to prevent pathogen invasion. It was known that the disruption on normal commensal microbiota composition or dysbiosis causes skin diseases, while the skin microbiota diversity itself is influenced by several factors, one of them is ethnicity. This study shows the influence of ethnicity factor in Papuans, Javanese, and Chinese descent young adults living in Jakarta on skin microbiome profiles. The microbiota genomic DNA are extracted from the face skin samples and sequenced with Next Generation Sequencing method to be further analyzed. The result shows that individuals with the same ethnic background share similar skin microbiome characteristics. The greatest skin microbiome alpha diversity is shown by the Papuans and the Chinese descent the smallest. Ethnicity factor that shows statistically significant differences in interindividual dissimilarities are independent of other intriguing factors such as age, geographical location, etc. Therefore the ethnic origin of individuals especially from three ethnics above is a factor to be considered in skin microbiome research and the skin microbiota composition can be used for potential future applications.

scholarly journals Chronic Diseases Associated with Malassezia Yeast

2021 ◽  
Author(s):  
Abdourahim Abdillah ◽  
Stéphane Ranque
Keyword(s):  
Colorectal Cancer ◽  
Chronic Diseases ◽  
Normal Skin ◽  
Human Diseases ◽  
Skin Microbiome ◽  
Body Site ◽  
Inflammatory Bowel ◽  
New Perspective ◽  
Malassezia Spp ◽  
Shed Light

Malassezia are lipid-dependent basidiomycetous yeast of the normal skin microbiome, although Malassezia DNA has been recently detected in other body sites and has been associated with cer-tain chronic human diseases. This new perspective raises many questions. Are these yeasts truly present in the investigated body site or were they contaminated by other body sites, adjacent or not? Does this DNA contamination come from living or dead yeast? If these yeasts are alive, do they belong to the resident mycobiota or are they transient colonizers which are not permanently established within these niches? And, finally, are these yeasts associated with certain chronic diseases or not? In an attempt to shed light on this knowledge gap, we critically re-viewed the 31 published studies focusing on the association of Malassezia spp. with chronic human diseases, including psoriasis, atopic dermatitis (AD), chronic rhinosinusitis (CRS), asthma, cystic fibrosis (CF), HIV infection, inflammatory bowel disease (IBD), colorectal cancer (CRC), and neurodegenerative diseases.

scholarly journals Features of the Skin Microbiota in Common Inflammatory Skin Diseases

Life ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 962
Author(s):  
Iva Ferček ◽  
Liborija Lugović-Mihić ◽  
Arjana Tambić-Andrašević ◽  
Diana Ćesić ◽  
Ana Gverić Grginić ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Seborrheic Dermatitis ◽  
Bacterial Species ◽  
Clinical Manifestations ◽  
Skin Microbiome ◽  
Bacterial Strains ◽  
Skin Microbiota ◽  
Inflammatory Skin Diseases ◽  
Bacterial Microbiome ◽  
Inflammatory Skin

Many relatively common chronic inflammatory skin diseases manifest on the face (seborrheic dermatitis, rosacea, acne, perioral/periorificial dermatitis, periocular dermatitis, etc.), thereby significantly impairing patient appearance and quality of life. Given the yet unexplained pathogenesis and numerous factors involved, these diseases often present therapeutic challenges. The term “microbiome” comprises the totality of microorganisms (microbiota), their genomes, and environmental factors in a particular environment. Changes in human skin microbiota composition and/or functionality are believed to trigger immune dysregulation, and consequently an inflammatory response, thereby playing a potentially significant role in the clinical manifestations and treatment of these diseases. Although cultivation methods have traditionally been used in studies of bacterial microbiome species, a large number of bacterial strains cannot be grown in the laboratory. Since standard culture-dependent methods detect fewer than 1% of all bacterial species, a metagenomic approach could be used to detect bacteria that cannot be cultivated. The skin microbiome exhibits spatial distribution associated with the microenvironment (sebaceous, moist, and dry areas). However, although disturbance of the skin microbiome can lead to a number of pathological conditions and diseases, it is still not clear whether skin diseases result from change in the microbiome or cause such a change. Thus far, the skin microbiome has been studied in atopic dermatitis, seborrheic dermatitis, psoriasis, acne, and rosacea. Studies on the possible association between changes in the microbiome and their association with skin diseases have improved the understanding of disease development, diagnostics, and therapeutics. The identification of the bacterial markers associated with particular inflammatory skin diseases would significantly accelerate the diagnostics and reduce treatment costs. Microbiota research and determination could facilitate the identification of potential causes of skin diseases that cannot be detected by simpler methods, thereby contributing to the design and development of more effective therapies.

scholarly journals Bottom-up ecology of the human microbiome: from metagenomes to metabolomes

2016 ◽  
Author(s):  
Daniel R. Garza ◽  
Marcel C. Van Verk ◽  
Martijn A. Huynen ◽  
Bas E. Dutilh
Keyword(s):  
High Resolution ◽  
Microbial Communities ◽  
Human Skin ◽  
Human Microbiome ◽  
Skin Microbiome ◽  
Body Site ◽  
Essential Factor ◽  
Metabolomics Data ◽  
Bottom Up ◽  
Site Specific

AbstractThe environmental metabolome is a dominant and essential factor shaping microbial communities. Thus, we hypothesized that metagenomic datasets could reveal the quantitative metabolic status of a given sample. Using a newly developed bottom-up ecology algorithm, we predicted high-resolution metabolomes of hundreds of metagenomic datasets from the human microbiome, revealing body-site specific metabolomes consistent with known metabolomics data, and suggesting that common cosmetics ingredients are some of the major metabolites shaping the human skin microbiome.

scholarly journals YOGURT; A NOVEL EXCIPIENT (WITH ITS LYSED BACTERIA, AMINO ACIDS, VITAMINS, FATTY ACIDS, AND MINERALS) FOR “TOPICAL DERMATOLOGICAL PRODUCTS” AND FOR “SKIN MICROBIOTA”

2020 ◽  
pp. 94-99
Author(s):  
M. ORHAN VAIZOGLU
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Antimicrobial Peptides ◽  
Stratum Corneum ◽  
Human Body ◽  
Conventional Therapy ◽  
Starter Cultures ◽  
Skin Microbiome ◽  
Research Groups ◽  
Skin Microbiota

In recent years various Microbiomes (Skin, Gut Lumen) of the human body have attracted the attention of different research groups. In the meantime it has been shown that the conventional therapy of different diseases by making use of antibiotics and similar antibacterial treatments may disturb the harmony of the Skin Microbiome, resulting in dysbiosis. There are efforts of using “live” or “tyndallized (lysed)” probiotics in order to treat different diseases of the skin. It is also known that amino acids are one of the important key elements of the skin. In this paper, a hypothesis for the utilization of yogurt as an excipient for various topical dermatological products will be proposed. Yogurt contains significant amounts of; Probiotics (starter cultures), Amino Acids, Vitamins, Minerals and various Fatty Acids (saturated, monounsaturated and polyunsaturated). Besides, it has been shown that Antimicrobial Peptides (Bacteriocins) are also present in yogurt. Yogurt could eventually be used as an excipient for the production of various topical dermatological products in order to deliver some of the above-mentioned constituents to the Stratum Corneum (Skin) locally.

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