scholarly journals Inactivation of the core cheVAWY chemotaxis genes disrupts chemotactic motility and organised biofilm formation in Campylobacter jejuni

2018 ◽  
Author(s):  
Mark Reuter ◽  
Eveline Ultee ◽  
Yasmin Toseafa ◽  
Andrew Tan ◽  
Arnoud H.M. van Vliet
Keyword(s):  
Campylobacter Jejuni ◽  
Biofilm Formation ◽  
Foodborne Pathogen ◽  
Flagellar Motility ◽  
The Core ◽  
Transmission Modes ◽  
Strain Nctc ◽  
Flagellar Assembly ◽  
Flagellar Proteins ◽  
Chemotaxis System

ABSTRACTFlagellar motility plays a central role in the bacterial foodborne pathogen Campylobacter jejuni, as flagellar motility is required for reaching the intestinal epithelium and subsequent colonisation or disease. Flagellar proteins also contribute strongly to biofilm formation during transmission. Chemotaxis is the process directing flagellar motility in response to attractant and repellent stimuli, but its role in biofilm formation of C. jejuni is not well understood. Here we show that inactivation of the core chemotaxis genes cheVAWY in C. jejuni strain NCTC 11168 affects both chemotactic motility and biofilm formation. Inactivation of any of the core chemotaxis genes (cheA, cheY, cheV or cheW) impaired chemotactic motility but did not affect flagellar assembly or growth. The ΔcheY mutant swam in clockwise loops, while complementation restored normal motility. Inactivation of the core chemotaxis genes interfered with the ability to form a discrete biofilm at the air-media interface, and the ΔcheY mutant displayed reduced dispersal/shedding of bacteria into the planktonic fraction. This suggests that while the chemotaxis system is not required for biofilm formation per se, it is necessary for organized biofilm formation. Hence interference with the Campylobacter chemotaxis system at any level disrupts optimal chemotactic motility and transmission modes such as biofilm formation.

scholarly journals Inactivation of the core cheVAWY chemotaxis genes disrupts chemotactic motility and organised biofilm formation in Campylobacter jejuni

2020 ◽  
Author(s):  
Mark Reuter ◽  
Eveline Ultee ◽  
Yasmin Toseafa ◽  
Andrew Tan ◽  
Arnoud H M van Vliet
Keyword(s):  
Campylobacter Jejuni ◽  
Biofilm Formation ◽  
Foodborne Pathogen ◽  
Flagellar Motility ◽  
The Core ◽  
Transmission Modes ◽  
Strain Nctc ◽  
Flagellar Assembly ◽  
Flagellar Proteins ◽  
Chemotaxis System

Abstract Flagellar motility plays a central role in the bacterial foodborne pathogen Campylobacter jejuni, as flagellar motility is required for reaching the intestinal epithelium and subsequent colonisation or disease. Flagellar proteins also contribute strongly to biofilm formation during transmission. Chemotaxis is the process directing flagellar motility in response to attractant and repellent stimuli, but its role in biofilm formation of C. jejuni is not well understood. Here we show that inactivation of the core chemotaxis genes cheVAWY in C. jejuni strain NCTC 11168 affects both chemotactic motility and biofilm formation. Inactivation of any of the core chemotaxis genes (cheA, cheY, cheV or cheW) impaired chemotactic motility but did not affect flagellar assembly or growth. The ∆cheY mutant swam in clockwise loops, while complementation restored normal motility. Inactivation of the core chemotaxis genes interfered with the ability to form a discrete biofilm at the air-media interface, and the ∆cheY mutant displayed reduced dispersal/shedding of bacteria into the planktonic fraction. This suggests that while the chemotaxis system is not required for biofilm formation per se, it is necessary for organized biofilm formation. Hence interference with the Campylobacter chemotaxis system at any level disrupts optimal chemotactic motility and transmission modes such as biofilm formation.

scholarly journals Modulation of Campylobacter jejuni Motility, Adhesion to Polystyrene Surfaces, and Invasion of INT407 Cells by Quorum-Sensing Inhibition

2020 ◽  
Vol 8 (1) ◽  
pp. 104 ◽  
Author(s):  
Katarina Šimunović ◽  
Dina Ramić ◽  
Changyun Xu ◽  
Sonja Smole Možina
Keyword(s):  
Quorum Sensing ◽  
Campylobacter Jejuni ◽  
Biofilm Formation ◽  
Vibrio Harveyi ◽  
Foodborne Pathogen ◽  
Wild Type ◽  
Natural Extracts ◽  
Ethanolic Extracts ◽  
Pure Compounds ◽  
Moderate Positive Correlation

Campylobacter jejuni is a major foodborne pathogen, and the LuxS-mediated quorum-sensing (QS) system influences its motility, biofilm formation, invasion, host colonization, and virulence. QS therefore represents a target for the control of C. jejuni. The aim of this study was to investigate the correlation of QS inhibition with changes in C. jejuni motility, adhesion to polystyrene surfaces, and adhesion to and invasion of INT407 cells. This was achieved by studying (i) the luxS-deficient mutant and (ii) treatment of C. jejuni with 20 natural extracts as six essential oils, 11 ethanolic extracts, and three pure compounds. Compared to the wild-type, the ΔluxS mutant showed decreased motility, adhesion to polystyrene surfaces, and invasion of INT407 cells. The anti-QS effects of the treatments (n = 15/20) were assayed using Vibrio harveyi BB170 bioluminescence. Moderate positive correlation was shown between C. jejuni QS reduction and reduced motility (τ = 0.492, p = 0.024), adhesion to polystyrene surfaces (τ = 0.419, p = 0.008), and invasion (r = 0.394, p = 0.068). The best overall effect was achieved with a Sedum rosea (roseroot) extract, with 96% QS reduction, a 1.41 log (96%) decrease in adhesion to polystyrene surfaces, and an 82% decrease in invasion. We show that natural extracts can reduce motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by C. jejuni through modulation of the LuxS (QS) system.

scholarly journals RNA Sequencing Data Sets Identifying Differentially Expressed Transcripts during Campylobacter jejuni Biofilm Formation

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Greg Tram ◽  
William P. Klare ◽  
Joel A. Cain ◽  
Basem Mourad ◽  
Stuart J. Cordwell ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Sequencing ◽  
Campylobacter Jejuni ◽  
Biofilm Formation ◽  
Foodborne Pathogen ◽  
Data Sets ◽  
Sequencing Data ◽  
Content Type ◽  
Differential Gene ◽  
Insight Into

Campylobacter jejuni is a foodborne pathogen and an important contributor to gastroenteritis in humans. C. jejuni readily forms biofilms which may play a role in the transmission of the pathogen from animals to humans. Herein, we present RNA sequencing data investigating differential gene expression in biofilm and planktonic C. jejuni. These data provide insight into pathways which may be important to biofilm formation in this organism.

scholarly journals Proteomic Analysis of Campylobacter jejuni 11168 Biofilms Reveals a Role for the Motility Complex in Biofilm Formation

2006 ◽  
Vol 188 (12) ◽  
pp. 4312-4320 ◽  
Author(s):  
Martin Kalmokoff ◽  
Patricia Lanthier ◽  
Tammy-Lynn Tremblay ◽  
Mary Foss ◽  
Peter C. Lau ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Stress Responses ◽  
Biofilm Formation ◽  
Expression Profiles ◽  
Flagellar Apparatus ◽  
Developed Countries ◽  
Solid Matrix ◽  
Flagellar Motility ◽  
Pellicle Formation ◽  
Insertional Inactivation

ABSTRACT Campylobacter jejuni remains the leading cause of bacterial gastroenteritis in developed countries, and yet little is known concerning the mechanisms by which this fastidious organism survives within its environment. We have demonstrated that C. jejuni 11168 can form biofilms on a variety of surfaces. Proteomic analyses of planktonic and biofilm-grown cells demonstrated differences in protein expression profiles between the two growth modes. Proteins involved in the motility complex, including the flagellins (FlaA, FlaB), the filament cap (FliD), the basal body (FlgG, FlgG2), and the chemotactic protein (CheA), all exhibited higher levels of expression in biofilms than found in stationary-phase planktonic cells. Additional proteins with enhanced expression included those involved in the general (GroEL, GroES) and oxidative (Tpx, Ahp) stress responses, two known adhesins (Peb1, FlaC), and proteins involved in biosynthesis, energy generation, and catabolic functions. An aflagellate flhA mutant not only lost the ability to attach to a solid matrix and form a biofilm but could no longer form a pellicle at the air-liquid interface of a liquid culture. Insertional inactivation of genes that affect the flagellar filament (fliA, flaA, flaB, flaG) or the expression of the cell adhesin (flaC) also resulted in a delay in pellicle formation. These findings demonstrate that the flagellar motility complex plays a crucial role in the initial attachment of C. jejuni 11168 to solid surfaces during biofilm formation as well as in the cell-to-cell interactions required for pellicle formation. Continued expression of the motility complex in mature biofilms is unusual and suggests a role for the flagellar apparatus in the biofilm phenotype.

scholarly journals Reduced Infection Efficiency of Phage NCTC 12673 on Non-Motile Campylobacter jejuni Strains Is Related to Oxidative Stress

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1955
Author(s):  
Jessica C. Sacher ◽  
Muhammad Afzal Javed ◽  
Clay S. Crippen ◽  
James Butcher ◽  
Annika Flint ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Surface ◽  
Campylobacter Jejuni ◽  
Diarrheal Disease ◽  
Foodborne Pathogen ◽  
Flagellar Motility ◽  
Phenotypic Analysis ◽  
Host Interactions ◽  
Host Interaction ◽  
Human Infections

Campylobacter jejuni is a Gram-negative foodborne pathogen that causes diarrheal disease and is associated with severe post-infectious sequelae. Bacteriophages (phages) are a possible means of reducing Campylobacter colonization in poultry to prevent downstream human infections. However, the factors influencing phage-host interactions must be better understood before this strategy can be predictably employed. Most studies have focused on Campylobacter phage binding to the host surface, with all phages classified as either capsule- or flagella-specific. Here we describe the characterization of a C. jejuni phage that requires functional flagellar glycosylation and motor genes for infection, without needing the flagella for adsorption to the cell surface. Through phage infectivity studies of targeted C. jejuni mutants, transcriptomic analysis of phage-resistant mutants, and genotypic and phenotypic analysis of a spontaneous phage variant capable of simultaneously overcoming flagellar gene dependence and sensitivity to oxidative stress, we have uncovered a link between oxidative stress, flagellar motility, and phage infectivity. Taken together, our results underscore the importance of understanding phage-host interactions beyond the cell surface and point to host oxidative stress state as an important and underappreciated consideration for future phage-host interaction studies.

scholarly journals The phosphorylated regulator of chemotaxis is crucial throughout biofilm biogenesis in Shewanella oneidensis

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Anne Boyeldieu ◽  
Amine Ali Chaouche ◽  
Moly Ba ◽  
Flora Ambre Honoré ◽  
Vincent Méjean ◽  
...  
Keyword(s):  
Solid Surface ◽  
Biofilm Formation ◽  
Essential Role ◽  
Shewanella Oneidensis ◽  
Swimming Direction ◽  
The Core ◽  
Diguanylate Cyclases ◽  
Biofilm Development ◽  
Chemotaxis System

AbstractThe core of the chemotaxis system of Shewanella oneidensis is made of the CheA3 kinase and the CheY3 regulator. When appropriated, CheA3 phosphorylates CheY3, which, in turn, binds to the rotor of the flagellum to modify the swimming direction. In this study, we showed that phosphorylated CheY3 (CheY3-P) also plays an essential role during biogenesis of the solid-surface-associated biofilm (SSA-biofilm). Indeed, in a ΔcheY3 strain, the formation of this biofilm is abolished. Using the phospho-mimetic CheY3D56E mutant, we showed that CheY-P is required throughout the biogenesis of the biofilm but CheY3 phosphorylation is independent of CheA3 during this process. We have recently found that CheY3 interacts with two diguanylate cyclases (DGCs) and with MxdA, the c-di-GMP effector, probably triggering exopolysaccharide synthesis by the Mxd machinery. Here, we discovered two additional DGCs involved in SSA-biofilm development and showed that one of them interacts with CheY3. We therefore propose that CheY3-P acts together with DGCs to control SSA-biofilm formation. Interestingly, two orthologous CheY regulators complement the biofilm defect of a ΔcheY3 strain, supporting the idea that biofilm formation could involve CheY regulators in other bacteria.

scholarly journals Listeria monocytogenes DNA Glycosylase AdlP Affects Flagellar Motility, Biofilm Formation, Virulence, and Stress Responses

2016 ◽  
Vol 82 (17) ◽  
pp. 5144-5152 ◽  
Author(s):  
Ting Zhang ◽  
Dongryeoul Bae ◽  
Chinling Wang
Keyword(s):  
Listeria Monocytogenes ◽  
Stress Responses ◽  
Transcriptional Repression ◽  
Biofilm Formation ◽  
Foodborne Pathogen ◽  
Dna Glycosylase ◽  
Flagellar Motility ◽  
Temperature Dependent ◽  
Bifunctional Protein ◽  
Content Type

ABSTRACTThe temperature-dependent alteration of flagellar motility gene expression is critical for the foodborne pathogenListeria monocytogenesto respond to a changing environment. In this study, a genetic determinant,L. monocytogenesf2365_0220(lmof2365_0220), encoding a putative protein that is structurally similar to theBacillus cereusalkyl base DNA glycosylase (AlkD), was identified. This determinant was involved in the transcriptional repression of flagellar motility genes and was namedadlP(encoding anAlkD-likeprotein [AdlP]). Deletion ofadlPactivated the expression of flagellar motility genes at 37°C and disrupted the temperature-dependent inhibition ofL. monocytogenesmotility. TheadlPnull strains demonstrated decreased survival in murine macrophage-like RAW264.7 cells and less virulence in mice. Furthermore, the deletion ofadlPsignificantly decreased biofilm formation and impaired the survival of bacteria under several stress conditions, including the presence of a DNA alkylation compound (methyl methanesulfonate), an oxidative agent (H2O2), and aminoglycoside antibiotics. Our findings strongly suggest thatadlPmay encode a bifunctional protein that transcriptionally represses the expression of flagellar motility genes and influences stress responses through its DNA glycosylase activity.IMPORTANCEWe discovered a novel protein that we namedAlkD-likeprotein (AdlP). This protein affected flagellar motility, biofilm formation, and virulence. Our data suggest that AdlP may be a bifunctional protein that represses flagellar motility genes and influences stress responses through its DNA glycosylase activity.

scholarly journals The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nicholas M. Negretti ◽  
Christopher R. Gourley ◽  
Prabhat K. Talukdar ◽  
Geremy Clair ◽  
Courtney M. Klappenbach ◽  
...  
Keyword(s):  
Host Cell ◽  
Campylobacter Jejuni ◽  
Foodborne Pathogen ◽  
Small Gtpase ◽  
Host Cells ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Cell Binding ◽  
Actin Reorganization ◽  
Cell Cytosol

AbstractCampylobacter jejuni is a foodborne pathogen that binds to and invades the epithelial cells lining the human intestinal tract. Maximal invasion of host cells by C. jejuni requires cell binding as well as delivery of the Cia proteins (Campylobacter invasion antigens) to the host cell cytosol via the flagellum. Here, we show that CiaD binds to the host cell protein IQGAP1 (a Ras GTPase-activating-like protein), thus displacing RacGAP1 from the IQGAP1 complex. This, in turn, leads to the unconstrained activity of the small GTPase Rac1, which is known to have roles in actin reorganization and internalization of C. jejuni. Our results represent the identification of a host cell protein targeted by a flagellar secreted effector protein and demonstrate that C. jejuni-stimulated Rac signaling is dependent on IQGAP1.

scholarly journals Mutagenic strategies against luxS gene affect the early stage of biofilm formation of Campylobacter jejuni

2021 ◽  
Author(s):  
Martin Teren ◽  
Ekaterina Shagieva ◽  
Lucie Vondrakova ◽  
Jitka Viktorova ◽  
Viviana Svarcova ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Biofilm Formation ◽  
Early Stage ◽  
Luxs Gene

The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168

2002 ◽  
Vol 269 (21) ◽  
pp. 5119-5136 ◽  
Author(s):  
Frank St. Michael ◽  
Christine M. Szymanski ◽  
Jianjun Li ◽  
Kenneth H. Chan ◽  
Nam Huan Khieu ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Strain Nctc
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