scholarly journals A normalized template matching method for improving spike detection in extracellular voltage recordings

2018 ◽  
Author(s):  
Keven Laboy-Juarez ◽  
Sei Ahn ◽  
Daniel E. Feldman
Keyword(s):  
Template Matching ◽  
Signal To Noise Ratio ◽  
Single Units ◽  
Detection Methods ◽  
Spike Detection ◽  
Matching Method ◽  
Voltage Threshold ◽  
Threshold Detection ◽  
High Firing

Spike sorting is the process of detecting and clustering action potential waveforms from extracellular voltage recordings to identify spikes of putative single neurons. Typically, spike detection is done using a fixed voltage threshold and shadow period, but this approach can lead to missed spikes during high firing rate epochs or noisy conditions. We developed a novel spike detection method utilizing a computationally simple form of template matching that efficiently detects spikes from candidate single units and is tolerant of high firing rates and electrical noise without a whitening filter. Template matching was based on a sliding cosine similarity between mean spike waveforms of candidate single units and the extracellular voltage signal. Performance was tested in whisker somatosensory cortex (S1) of anesthetized mice in vivo. The method consistently detected whisker-evoked spikes that were missed by a standard fixed voltage threshold. Detection was most improved for spikes evoked by strong stimuli (40-70% increase), less improved for weaker stimuli, and unchanged for spontaneous spiking. This reflected the failure of standard detection during spatiotemporally dense spiking. Template-based detection revealed higher signal-to-noise ratio for sensory responses and sharper sensory tuning. Thus, this template matching method (and other model-based spike detection methods) critically improve the quantification of single-unit spiking activity.

scholarly journals Spike Sorting of Muscle Spindle Afferent Nerve Activity Recorded with Thin-Film Intrafascicular Electrodes

2010 ◽  
Vol 2010 ◽  
pp. 1-13 ◽  
Author(s):  
Milan Djilas ◽  
Christine Azevedo-Coste ◽  
David Guiraud ◽  
Ken Yoshida
Keyword(s):  
Thin Film ◽  
Muscle Spindle ◽  
Signal To Noise Ratio ◽  
Muscle Length ◽  
Detection Scheme ◽  
Threshold Detection ◽  
Complex Wavelets ◽  
Intrafascicular Electrodes ◽  
Stimulation System

Afferent muscle spindle activity in response to passive muscle stretch was recorded in vivo using thin-film longitudinal intrafascicular electrodes. A neural spike detection and classification scheme was developed for the purpose of separating activity of primary and secondary muscle spindle afferents. The algorithm is based on the multiscale continuous wavelet transform using complex wavelets. The detection scheme outperforms the commonly used threshold detection, especially with recordings having low signal-to-noise ratio. Results of classification of units indicate that the developed classifier is able to isolate activity having linear relationship with muscle length, which is a step towards online model-based estimation of muscle length that can be used in a closed-loop functional electrical stimulation system with natural sensory feedback.

A Detection Method for Overlapped Spikes

2011 ◽  
Vol 267 ◽  
pp. 530-535
Author(s):  
Jia Qi ◽  
Min Dai ◽  
Gang Zheng ◽  
Tong Tong Liu
Keyword(s):  
Detection Method ◽  
Signal To Noise Ratio ◽  
False Negative ◽  
False Negative Rate ◽  
Energy Operator ◽  
Detection Methods ◽  
Signal To Noise ◽  
Threshold Detection ◽  
Noise Ratio ◽  
Nonlinear Energy

A new spike detection method is proposed in order to detect the overlapped spikes. In order to avoid missing overlapped spikes, the method adds threshold detection based on window detection method. Moreover, nonlinear energy operator is introduced to make the method strong even under low signal-to-noise ratio situation. In addition, the method solves the repeated detection problem by estimating slopes. Experiments show that the method is good for any occasion whatever the low signal-to-noise ratio or baseline wander. Especially for the overlapped spikes detection, it has much lower false-negative-rate than other traditional detection methods.

scholarly journals Author Correction: A normalized template matching method for improving spike detection in extracellular voltage recordings

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Keven J. Laboy-Juárez ◽  
Seoiyoung Ahn ◽  
Daniel E. Feldman
Keyword(s):  
Template Matching ◽  
Spike Detection ◽  
Matching Method

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Detectivity optimization to detect of ultraweak light fluxes with an EM-CCD as binary photon counter array

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ibtissame Khaoua ◽  
Guillaume Graciani ◽  
Andrey Kim ◽  
François Amblard
Keyword(s):  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
Photon Counting ◽  
Detection Methods ◽  
Strong Nonlinearity ◽  
Wide Range ◽  
Depth Analysis ◽  
Spatially Extended ◽  
Extended Sources ◽  
Photon Counting Mode

AbstractFor a wide range of purposes, one faces the challenge to detect light from extremely faint and spatially extended sources. In such cases, detector noises dominate over the photon noise of the source, and quantum detectors in photon counting mode are generally the best option. Here, we combine a statistical model with an in-depth analysis of detector noises and calibration experiments, and we show that visible light can be detected with an electron-multiplying charge-coupled devices (EM-CCD) with a signal-to-noise ratio (SNR) of 3 for fluxes less than $$30\,{\text{photon}}\,{\text{s}}^{ - 1} \,{\text{cm}}^{ - 2}$$ 30 photon s - 1 cm - 2 . For green photons, this corresponds to 12 aW $${\text{cm}}^{ - 2}$$ cm - 2 ≈ $$9{ } \times 10^{ - 11}$$ 9 × 10 - 11 lux, i.e. 15 orders of magnitude less than typical daylight. The strong nonlinearity of the SNR with the sampling time leads to a dynamic range of detection of 4 orders of magnitude. To detect possibly varying light fluxes, we operate in conditions of maximal detectivity $${\mathcal{D}}$$ D rather than maximal SNR. Given the quantum efficiency $$QE\left( \lambda \right)$$ Q E λ of the detector, we find $${ \mathcal{D}} = 0.015\,{\text{photon}}^{ - 1} \,{\text{s}}^{1/2} \,{\text{cm}}$$ D = 0.015 photon - 1 s 1 / 2 cm , and a non-negligible sensitivity to blackbody radiation for T > 50 °C. This work should help design highly sensitive luminescence detection methods and develop experiments to explore dynamic phenomena involving ultra-weak luminescence in biology, chemistry, and material sciences.

Weak Transient Electromagnetic Radiation Signal Detection Method Considering the New Watershed Image Segmentation Algorithm

2019 ◽  
Vol 34 (03) ◽  
pp. 2054009
Author(s):  
Wenjun Huo ◽  
Peng Chu ◽  
Kai Wang ◽  
Liangting Fu ◽  
Zhigang Niu ◽  
...  
Keyword(s):  
Electromagnetic Radiation ◽  
Cross Correlation ◽  
Detection Efficiency ◽  
Signal To Noise Ratio ◽  
Estimation Method ◽  
Detection Methods ◽  
Signal To Noise ◽  
Long Distance ◽  
Transient Electromagnetic ◽  
Noise Ratio

In order to study the detection methods of weak transient electromagnetic radiation signals, a detection algorithm integrating generalized cross-correlation and chaotic sequence prediction is proposed in this paper. Based on the dual-antenna test and cross-correlation information estimation method, the detection of aperiodic weak discharge signals under low signal-to-noise ratio is transformed into the estimation of periodic delay parameters, and the noise is reduced at the same time. The feasibility of this method is verified by simulation and experimental analysis. The results show that under the condition of low signal-to-noise ratio, the integrated method can effectively suppress the influence of 10 noise disturbances. It has a high detection probability for weak transient electromagnetic radiation signals, and needs fewer pulse accumulation times, which improves the detection efficiency and is more suitable for long-distance detection of weak electromagnetic radiation sources.

scholarly journals High-speed high-resolution laser diode-based photoacoustic microscopy for in vivo microvasculature imaging

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Xiufeng Li ◽  
Victor T C Tsang ◽  
Lei Kang ◽  
Yan Zhang ◽  
Terence T W Wong
Keyword(s):  
High Resolution ◽  
High Speed ◽  
High Performance ◽  
Continuous Wave ◽  
Signal To Noise Ratio ◽  
Cost Effective ◽  
High Signal ◽  
Photoacoustic Microscopy ◽  
Mouse Ear

AbstractLaser diodes (LDs) have been considered as cost-effective and compact excitation sources to overcome the requirement of costly and bulky pulsed laser sources that are commonly used in photoacoustic microscopy (PAM). However, the spatial resolution and/or imaging speed of previously reported LD-based PAM systems have not been optimized simultaneously. In this paper, we developed a high-speed and high-resolution LD-based PAM system using a continuous wave LD, operating at a pulsed mode, with a repetition rate of 30 kHz, as an excitation source. A hybrid scanning mechanism that synchronizes a one-dimensional galvanometer mirror and a two-dimensional motorized stage is applied to achieve a fast imaging capability without signal averaging due to the high signal-to-noise ratio. By optimizing the optical system, a high lateral resolution of 4.8 μm has been achieved. In vivo microvasculature imaging of a mouse ear has been demonstrated to show the high performance of our LD-based PAM system.

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