scholarly journals Haematological parameters and plasma levels of 8-iso-prostaglandin F2α in malaria-sickle cell co-morbidity: A cross sectional study

2018 ◽  
Author(s):  
Enoch Aninagyei ◽  
Emmanuel Doku Tetteh ◽  
Josephine Banini ◽  
Emmanuel Nani ◽  
Patrick Adu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sickle Cell ◽  
Parasite Density ◽  
Plasma Levels ◽  
Prostaglandin F2α ◽  
Inflammatory Biomarkers ◽  
Haematological Parameters ◽  
Co Morbidity ◽  
Haemoglobin Type ◽  
Haematological Profile

AbstractIntroductionMalaria and sickle cell disease (SCD) co-morbidity have previously been reported in Ghana. However, there is paucity of data on haematological profiles and oxidative stress in comorbidity states. This study identified novel inflammatory biomarkers associated with malaria in SCD and analyzed the levels of 8-iso-prostaglandin F2α oxidative stress biomarker in malaria-SCD co-morbidity in Ghanaian patients.MethodsBlood (5ml) was collected from malaria patients into K3-EDTA tube. Malaria parasites speciation and quantification were then done according WHO guidelines. All eligible samples were assayed for haematological profile, sickle cell phenotyping, infectious markers (hepatitis B, hepatitis C, syphilis and HIV 1&2) and plasma levels of 8-epi-prostaglandin F2α..ResultsPrevalence of malaria in SCD (malaria-SCD) was 13.4% (45/335). Male: female ratio was 0.8:1 (X2=1.43, p=0.231). Mean ages for malaria in normal haemoglobin type (malaria-HbAA) and malaria-SCD were 12.79±4.91 and 11.56±3.65 years respectively (p=0.048). Geometric mean of parasite density was higher in malaria-HbAA (20394 parasites/μl vs. 9990 parasites/μl, p=0.001) whilst mean body temperature was higher in malaria-SCD (39.0±0.87°C vs. 37.9±1.15°C, p=0.001). Mean leukocytes, lymphocytes, eosinophils, monocytes, platelets and platelet indices values were significantly elevated in malaria-SCD. Significant reduction in RBC and RBC indices in malaria-SCD were also observed. Eosinophils-to-basophils ratio (EBR) and monocytes-to-basophils ratio (MBR) were novel cellular inflammatory biomarkers which could predict malaria in SCD. The sensitivities of cut-off values of EBR>14, MBR>22 and combined use of EBR>14 and MBR>22 were 79.55%, 84.09% and 91.11% respectively. Mean 8-iso-prostaglandin F2α was 338.1pg/ml in malaria-HbAA and 643.8pg/ml in malaria-SCD (p=0.001). 8-iso-prostaglandin F2α correlated with parasite density (r=0.787, p=0.001), temperature (r=0.566, p=0.001) and leucocytes (r=0.573, p=0.001) and negatively correlated with RBC (r=−0.476, p=0.003), haemoglobin (r=−0.851, p=0.001) and haematocrit (r=−0.735, p=0.001).ConclusionPlasmodium falciparum parasitaemia increases oxidative damage and causes derangement haematological parameters. Cut of values of EBR>14 and MBR>22 could predict malaria in SCD.

scholarly journals Study the Haematological parameters and Oxidative stress before and after disbudding in Calves

2021 ◽  
pp. 62-69
Author(s):  
Shiv Swaroop ◽  
Pramanik PS ◽  
Singh KD ◽  
Subodh Kumar ◽  
Verma AK
Keyword(s):  
Oxidative Stress ◽  
Animal Husbandry ◽  
Lipid Peroxides ◽  
Haematological Parameters ◽  
Veterinary Science ◽  
Treatment Groups ◽  
Significant Difference ◽  
Haematological Profile ◽  
Before And After ◽  
And Oxidative Stress

The aim of the present investigation was to examine the haematological profile and oxidative stress before and after disbudding in calves. The study was carried out at Livestock Farm Complex, College of Veterinary Science and Animal Husbandry, ANDUAT, Kumarganj, Ayodhya and nearby villages. Twenty four cattle calves were utilized to study haematological parameters that are Haemoglobin (Hb (gm/dl), Neutrophil (%), Basophil (%), PCV (%), Lymphocyte (%), Monocyte (%), TLC (103/ µl), Eosinophil (%), N/L ratio) and oxidative stress before and after disbudding in to four groups viz. control (T0), lignocaine (TL), tremadol (TT) and lignocaine+tremadol (TL-T). After medication calves were disbudded by hot iron method. The blood samples were collected for haematological parameters & for estimation of LPO (lipid peroxides) as per method described by [1] just before (0h) and after disbudding on 6h, 24h and 72h. The results showed that, at 24h, T0 group showed significantly lowest Hb (10.66±0.49%) which was statistically similar to TT and TL-T groups. At 0h, 6h and 72h means of PCV% were significantly different among treatment groups. The lowest lymphocytes (64.00±1.00) was observed in TT group at 6h. The T0 group showed significantly lowest basophil (0.00±0.00) which was statistically similar to TT and TL-T groups but significantly different from TL group at 6h. At the same interval, T0 group showed significantly lowest monocytes (4.66±0.61) which was significantly different with TL, TT and TL-T groups. At 6h, T0 group showed significantly lowest N/ L ratio (0.15±0.01) which was statistically similar to TT and TL-T groups but significantly different from TL group. At 0h, 6h and 24h LPO was significantly different among groups. At 0h, T0 group showed significantly highest (106.59±0.63) which was significantly different from TL, TT and TL-T groups. It may be concluded that no significant difference was observed in eosinophil%, TLC, neutrophil% and monocyte% counts between control and treatment groups. However, Hb%, basophil%, PCV% and lymphocyte% were significantly different between control and treatment groups. N/L ratio was significant only at 6h. Most of the cases TL-T group showed comparatively better performance. At 0h, 6h and 24h LPO was significantly different among groups. At 0h, T0 group showed significantly highest (106.59±0.63) which was significantly different from TL, TT and TL-T groups.

Inflammatory Biomarkers in Atrial Fibrillation

2019 ◽  
Vol 26 (5) ◽  
pp. 837-854 ◽  
Author(s):  
Effimia Zacharia ◽  
Nikolaos Papageorgiou ◽  
Adam Ioannou ◽  
Gerasimos Siasos ◽  
Spyridon Papaioannou ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Plasma Levels ◽  
Large Scale ◽  
Inflammatory Biomarkers ◽  
Inflammatory Pathways ◽  
Inflammatory State ◽  
Anti Inflammatory ◽  
Underlying Mechanisms

During the last few years, a significant number of studies have attempted to clarify the underlying mechanisms that lead to the presentation of atrial fibrillation (AF). Inflammation is a key component of the pathophysiological processes that lead to the development of AF; the amplification of inflammatory pathways triggers AF, and, in tandem, AF increases the inflammatory state. Indeed, the plasma levels of several inflammatory biomarkers are elevated in patients with AF. In addition, the levels of specific inflammatory biomarkers may provide information regarding to the AF duration. Several small studies have assessed the role of anti-inflammatory treatment in atrial fibrillation but the results have been contradictory. Large-scale studies are needed to evaluate the role of inflammation in AF and whether anti-inflammatory medications should be routinely administered to patients with AF.

scholarly journals Sickle Cell Disease: Role of Oxidative Stress and Antioxidant Therapy

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 296
Author(s):  
Rosa Vona ◽  
Nadia Maria Sposi ◽  
Lorenza Mattia ◽  
Lucrezia Gambardella ◽  
Elisabetta Straface ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Globin Gene ◽  
Organ Damage ◽  
Cell Disease ◽  
Vessel Occlusion ◽  
Antioxidant Agents ◽  
Oxidant Status

Sickle cell disease (SCD) is the most common hereditary disorder of hemoglobin (Hb), which affects approximately a million people worldwide. It is characterized by a single nucleotide substitution in the β-globin gene, leading to the production of abnormal sickle hemoglobin (HbS) with multi-system consequences. HbS polymerization is the primary event in SCD. Repeated polymerization and depolymerization of Hb causes oxidative stress that plays a key role in the pathophysiology of hemolysis, vessel occlusion and the following organ damage in sickle cell patients. For this reason, reactive oxidizing species and the (end)-products of their oxidative reactions have been proposed as markers of both tissue pro-oxidant status and disease severity. Although more studies are needed to clarify their role, antioxidant agents have been shown to be effective in reducing pathological consequences of the disease by preventing oxidative damage in SCD, i.e., by decreasing the oxidant formation or repairing the induced damage. An improved understanding of oxidative stress will lead to targeted antioxidant therapies that should prevent or delay the development of organ complications in this patient population.

scholarly journals Inheritance of the Bantu/Benin haplotype causes less severe hemolytic and oxidative stress in sickle cell anemia patients treated with hydroxycarbamide

2016 ◽  
Vol 61 (7) ◽  
pp. 605-611 ◽  
Author(s):  
Jéssika V Okumura ◽  
Danilo G H Silva ◽  
Lidiane S Torres ◽  
Edis Belini-Junior ◽  
Willian M Barberino ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
And Oxidative Stress

scholarly journals The Effect of a Selective Inhibitor of Phosphodiesterase-9 on Oxidative Stress, Inflammation and Cytotoxicity in Neutrophils from Patients with Sickle Cell Anaemia

2015 ◽  
Vol 118 (4) ◽  
pp. 271-278 ◽  
Author(s):  
Maritza Cavalcante Barbosa ◽  
Talyta Ellen de Jesus dos Santos ◽  
Thayna Nogueira dos Santos ◽  
Alano Martins Pedrosa ◽  
Darcielle Bruna Dias Elias ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sickle Cell ◽  
Sickle Cell Anaemia ◽  
Selective Inhibitor

scholarly journals Urinary 1-Hydroxypyrene is Associated with Oxidative Stress and Inflammatory Biomarkers in Acute Myocardial Infarction

2014 ◽  
Vol 11 (9) ◽  
pp. 9024-9037 ◽  
Author(s):  
Fernando Freitas ◽  
Natália Brucker ◽  
Juliano Durgante ◽  
Guilherme Bubols ◽  
Rachel Bulcão ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Inflammatory Biomarkers

scholarly journals Oxidative stress in sickle cell disease; pathophysiology and potential implications for disease management

2011 ◽  
Vol 86 (6) ◽  
pp. 484-489 ◽  
Author(s):  
Erfan Nur ◽  
Bart J. Biemond ◽  
Hans-Martin Otten ◽  
Dees P. Brandjes ◽  
John-John B. Schnog ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sickle Cell Disease ◽  
Disease Management ◽  
Sickle Cell ◽  
Cell Disease
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