scholarly journals A complex symbiosis involving within species variation in the response ofDictyosteliumamoebae toBurkholderiabacteria

2018 ◽  
Author(s):  
Longfei Shu ◽  
Debra A. Brock ◽  
Katherine S. Geista ◽  
Jacob W. Miller ◽  
David C. Queller ◽  
...  
Keyword(s):  
Dictyostelium Discoideum ◽  
Species Variation ◽  
Bacterial Symbionts ◽  
Disease Dynamics ◽  
Fitness Costs ◽  
Social Amoeba ◽  
Host Fitness ◽  
Symbiont Type ◽  
The Social

AbstractRecent symbioses, particularly facultative ones, are well suited for unravelling the evolutionary give and take between partners. Here we look at variation in wild-collected samples of the social amoebaDictyostelium discoideumand their relationships with bacterial symbionts,Burkholderia hayleyellaandBurkholderia agricolaris. Only about a third of field-collected amoebae carry a symbiont. We cured and cross-infectedD. discoideumhosts with different symbiont association histories and then compared the responses of the amoebae to each symbiont type. Before curing, field-collected clones did not vary significantly in overall fitness, but infected hosts produced morphologically different multicellular structures. After curing and re-infecting, host fitness declined overall. However, naturalB. hayleyellahosts suffered fewer fitness costs when re-infected withB. hayleyella,indicating that they have evolved mechanisms to tolerate their naturally acquired symbiont. Exploring relationships between endosymbionts and hosts that vary within species may also reveal much about disease dynamics.

scholarly journals Symbiont location, host fitness, and possible coadaptation in a symbiosis between social amoebae and bacteria

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Longfei Shu ◽  
Debra A Brock ◽  
Katherine S Geist ◽  
Jacob W Miller ◽  
David C Queller ◽  
...  
Keyword(s):  
Dictyostelium Discoideum ◽  
Host Responses ◽  
Bacterial Symbionts ◽  
Disease Dynamics ◽  
Fitness Costs ◽  
Social Amoeba ◽  
Host Fitness ◽  
The Social ◽  
Natural Isolates ◽  
Social Amoebae

Recent symbioses, particularly facultative ones, are well suited for unravelling the evolutionary give and take between partners. Here we look at variation in natural isolates of the social amoeba Dictyostelium discoideum and their relationships with bacterial symbionts, Burkholderia hayleyella and Burkholderia agricolaris. Only about a third of field-collected amoebae carry a symbiont. We cured and cross-infected amoebae hosts with different symbiont association histories and then compared host responses to each symbiont type. Before curing, field-collected clones did not vary significantly in overall fitness, but infected hosts produced morphologically different multicellular structures. After curing and reinfecting, host fitness declined. However, natural B. hayleyella hosts suffered fewer fitness costs when reinfected with B. hayleyella, indicating that they have evolved mechanisms to tolerate their symbiont. Our work suggests that amoebae hosts have evolved mechanisms to tolerate specific acquired symbionts; exploring host-symbiont relationships that vary within species may provide further insights into disease dynamics.

scholarly journals Cooperation and conflict in the social amoeba Dictyostelium discoideum

2019 ◽  
Vol 63 (8-9-10) ◽  
pp. 371-382
Author(s):  
James M. Medina ◽  
P.M. Shreenidhi ◽  
Tyler J. Larsen ◽  
David C. Queller ◽  
Joan E. Strassmann
Keyword(s):  
Dictyostelium Discoideum ◽  
Fruiting Body ◽  
Sexual Cycle ◽  
Evolution Of Cooperation ◽  
Bacterial Symbionts ◽  
Spore Dispersal ◽  
Social Amoeba ◽  
The Social ◽  
The Relationship ◽  
Insight Into

The social amoeba Dictyostelium discoideum has provided considerable insight into the evolution of cooperation and conflict. Under starvation, D. discoideum amoebas cooperate to form a fruiting body comprised of hardy spores atop a stalk. The stalk development is altruistic because stalk cells die to aid spore dispersal. The high relatedness of cells in fruiting bodies in nature implies that this altruism often benefits relatives. However, since the fruiting body forms through aggregation there is potential for non-relatives to join the aggregate and create conflict over spore and stalk fates. Cheating is common in chimeras of social amoebas, where one genotype often takes advantage of the other and makes more spores. This social conflict is a significant force in nature as indicated by rapid rates of adaptive evolution in genes involved in cheating and its resistance. However, cheating can be prevented by high relatedness, allorecognition via tgr genes, pleiotropy and evolved resistance. Future avenues for the study of cooperation and conflict in D. discoideum include the sexual cycle as well as the relationship between D. discoideum and its bacterial symbionts. D. discoideum’s tractability in the laboratory as well as its uncommon mode of aggregative multicellularity have established it as a promising model for future studies of cooperation and conflict.

scholarly journals Fitness costs and benefits vary for two facultative Burkholderia symbionts of the social amoeba, Dictyostelium discoideum

2019 ◽  
Vol 9 (17) ◽  
pp. 9878-9890 ◽  
Author(s):  
Justine R. Garcia ◽  
Tyler J. Larsen ◽  
David C. Queller ◽  
Joan E. Strassmann
Keyword(s):  
Dictyostelium Discoideum ◽  
Costs And Benefits ◽  
Fitness Costs ◽  
Social Amoeba ◽  
The Social

scholarly journals Amino Acid Repeats Cause Extraordinary Coding Sequence Variation in the Social Amoeba Dictyostelium discoideum

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e46150 ◽  
Author(s):  
Clea Scala ◽  
Xiangjun Tian ◽  
Natasha J. Mehdiabadi ◽  
Margaret H. Smith ◽  
Gerda Saxer ◽  
...  
Keyword(s):  
Amino Acid ◽  
Dictyostelium Discoideum ◽  
Sequence Variation ◽  
Social Amoeba ◽  
Coding Sequence ◽  
Amino Acid Repeats ◽  
The Social

scholarly journals Microbe Profile: Dictyostelium discoideum: model system for development, chemotaxis and biomedical research

Microbiology ◽  
2021 ◽  
Author(s):  
Catherine J. Pears ◽  
Julian D. Gross
Keyword(s):  
Pattern Formation ◽  
Cell Motility ◽  
Dictyostelium Discoideum ◽  
Biomedical Research ◽  
Model System ◽  
Developmental Pattern ◽  
Social Amoeba ◽  
The Social ◽  
Host Pathogen ◽  
Soil Amoeba

The social amoeba Dictyostelium discoideum is a versatile organism that is unusual in alternating between single-celled and multi-celled forms. It possesses highly-developed systems for cell motility and chemotaxis, phagocytosis, and developmental pattern formation. As a soil amoeba growing on microorganisms, it is exposed to many potential pathogens; it thus provides fruitful ways of investigating host-pathogen interactions and is emerging as an influential model for biomedical research.

scholarly journals Role of epigenetics in unicellular to multicellular transition in Dictyostelium

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Simon Yuan Wang ◽  
Elizabeth Ann Pollina ◽  
I-Hao Wang ◽  
Lindsay Kristina Pino ◽  
Henry L. Bushnell ◽  
...  
Keyword(s):  
Dictyostelium Discoideum ◽  
Orthologous Gene ◽  
Chromatin Accessibility ◽  
Critical Event ◽  
H3k4 Methylation ◽  
Social Amoeba ◽  
The Social ◽  
Conserved Genes ◽  
Multicellular Organisms

Abstract Background The evolution of multicellularity is a critical event that remains incompletely understood. We use the social amoeba, Dictyostelium discoideum, one of the rare organisms that readily transits back and forth between both unicellular and multicellular stages, to examine the role of epigenetics in regulating multicellularity. Results While transitioning to multicellular states, patterns of H3K4 methylation and H3K27 acetylation significantly change. By combining transcriptomics, epigenomics, chromatin accessibility, and orthologous gene analyses with other unicellular and multicellular organisms, we identify 52 conserved genes, which are specifically accessible and expressed during multicellular states. We validated that four of these genes, including the H3K27 deacetylase hdaD, are necessary and that an SMC-like gene, smcl1, is sufficient for multicellularity in Dictyostelium. Conclusions These results highlight the importance of epigenetics in reorganizing chromatin architecture to facilitate multicellularity in Dictyostelium discoideum and raise exciting possibilities about the role of epigenetics in the evolution of multicellularity more broadly.

scholarly journals (Auto)Biographical reflections on the contributions of William F. Loomis (1940-2016) to Dictyostelium biology

2019 ◽  
Vol 63 (8-9-10) ◽  
pp. 343-357
Author(s):  
Adam Kuspa ◽  
Gad Shaulsky
Keyword(s):  
Cell Differentiation ◽  
Molecular Biology ◽  
Genetic Control ◽  
Dictyostelium Discoideum ◽  
University Of California ◽  
High Dimensional ◽  
Social Amoeba ◽  
The Social ◽  
The University ◽  
High Dimensional Datasets

William Farnsworth Loomis studied the social amoeba Dictyostelium discoideum for more than fifty years as a professor of biology at the University of California, San Diego, USA. This biographical reflection describes Dr. Loomis’ major scientific contributions to the field within a career arc that spanned the early days of molecular biology up to the present day where the acquisition of high-dimensional datasets drive research. Dr. Loomis explored the genetic control of social amoeba development, delineated mechanisms of cell differentiation, and significantly advanced genetic and genomic technology for the field. The details of Dr. Loomis’ multifaceted career are drawn from his published work, from an autobiographical essay that he wrote near the end of his career and from extensive conversations between him and the two authors, many of which took place on the deck of his beachfront home in Del Mar, California.

scholarly journals Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum

Cells ◽  
2017 ◽  
Vol 6 (3) ◽  
pp. 18 ◽  
Author(s):  
Eunice Domínguez-Martín ◽  
Elena Cardenal-Muñoz ◽  
Jason King ◽  
Thierry Soldati ◽  
Roberto Coria ◽  
...  
Keyword(s):  
Dictyostelium Discoideum ◽  
Social Amoeba ◽  
The Social
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