scholarly journals StPOPA, encoding an anionic peroxidase in Solanum tuberosum, enhances resistance against Phytophthora infestans

2018 ◽  
Author(s):  
Yu Yang ◽  
Rui Jiang ◽  
Hongyang Wang ◽  
Zhendong Tian ◽  
Conghua Xie
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Early Stage ◽  
Mechanical Damage ◽  
Potato Late Blight ◽  
Oxygen Species ◽  
Limited Information ◽  
Gene Encoding ◽  
Signal Molecule ◽  
Anionic Peroxidase

AbstractPotato late blight, caused by Phytophthora infestans, is one of the major threats affecting the quality and output of potato all over the world. Reactive oxygen species (ROS) acted as a signal molecule to transmit signals in plants at the early stage of disease infection, and it could induce disease resistance of the plant, including potato late blight. Anionic peroxidases in many plants were reported to be involved in defense to disease. However, limited information about anionic peroxidase genes is available for the potato. Here, we reported that the expression of the StPOPA, a gene encoding a suberization-associated anionic peroxidase, was associated with resistance in potato against P. infestans. The StPOPA gene was induced by P. infestans infection, mechanical damage, jasmonic acid and ethylene treatment. Overexpression of the StPOPA gene in potato enhanced the resistance against P. infestans via promoting the accumulation of callose in the cell wall and ROS in the cytoplasm, which restricted the infection and spreading of the disease possibly by purposeful programmed cell death. Taken together, our results suggested that the StPOPA gene contributed to potato immunity against P. infestans and this gene could be used for the genetic improvement of resistance against potato late blight.

Discerning strains of Phytophthora infestans the causative agent of potato late blight by SSCP analysis of virulence genes

2018 ◽  
Vol 34 (6) ◽  
pp. 4-11
Author(s):  
V.K. Chizhik ◽  
◽  
E.A. Sokolova ◽  
V.V. Martynov ◽  
M.A. Kuznetsova ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Causative Agent ◽  
Virulence Genes ◽  
Potato Late Blight ◽  
Sscp Analysis

scholarly journals Modeling of Yield Losses Caused by Potato Late Blight on Eight Cultivars with Different Levels of Resistance to Phytophthora infestans

Plant Disease ◽  
2012 ◽  
Vol 96 (7) ◽  
pp. 935-942 ◽  
Author(s):  
Toky Rakotonindraina ◽  
Jean-Éric Chauvin ◽  
Roland Pellé ◽  
Robert Faivre ◽  
Catherine Chatot ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Relative Yield ◽  
Weather Conditions ◽  
Potato Late Blight ◽  
Yield Losses ◽  
Predictive Quality ◽  
Wide Range ◽  
Cultivar Susceptibility

The Shtienberg model for predicting yield loss caused by Phytophthora infestans in potato was developed and parameterized in the 1990s in North America. The predictive quality of this model was evaluated in France for a wide range of epidemics under different soil and weather conditions and on cultivars different than those used to estimate its parameters. A field experiment was carried out in 2006, 2007, 2008, and 2009 in Brittany, western France to assess late blight severity and yield losses. The dynamics of late blight were monitored on eight cultivars with varying types and levels of resistance. The model correctly predicted relative yield losses (efficiency = 0.80, root mean square error of prediction = 13.25%, and bias = –0.36%) as a function of weather and the observed disease dynamics for a wide range of late blight epidemics. In addition to the evaluation of the predictive quality of the model, this article provides a dataset that describes the development of various late blight epidemics on potato as a function of weather conditions, fungicide regimes, and cultivar susceptibility. Following this evaluation, the Shtienberg model can be used with confidence in research and development programs to better manage potato late blight in France.

scholarly journals Complete mitogenome mapping of potato late blight pathogen, Phytophthora infestans A2 mating type

2017 ◽  
Vol 2 (1) ◽  
pp. 90-91
Author(s):  
Virupaksh U. Patil ◽  
G. Vanishree ◽  
Debasis Pattanayak ◽  
Sanjeev Sharma ◽  
Vinay Bhardwaj ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Mating Type ◽  
Potato Late Blight ◽  
Complete Mitogenome ◽  
Late Blight Pathogen

scholarly journals EIN2-mediated signaling is involved in pre-invasion defense in Nicotiana benthamiana against potato late blight pathogen, Phytophthora infestans

2017 ◽  
Vol 12 (4) ◽  
pp. e1300733 ◽  
Author(s):  
Soriya Rin ◽  
Yuri Mizuno ◽  
Yusuke Shibata ◽  
Mayuka Fushimi ◽  
Shinpei Katou ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Nicotiana Benthamiana ◽  
Potato Late Blight ◽  
Late Blight Pathogen

scholarly journals Respuesta de biosurfactantes producidos por Pseudomonas fluorescens para el control de la gota de la papa Phytophthora infestans (Mont) de Bary, bajo condiciones controlada.

2010 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Hugo F. Rivera ◽  
Erika P. Martínez ◽  
Jairo A. Osorio ◽  
Edgar Martínez
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Pseudomonas Fluorescens ◽  
Negative Impact ◽  
Potato Late Blight ◽  
Pathogen Resistance ◽  
Production Costs ◽  
In Vitro Assays

<p>Phytophthora infestans (Mont.) de Bary, agente causal de la gota de la papa, es considerado la principal limitante de la producción de este cultivo en Colombia. El control habitual del patógeno se realiza con fungicidas de tipo sistémico, que incrementan los costos de producción, pueden inducir la resistencia del patógeno y tiene un impacto negativo en el ambiente. Por tanto, se llevó a cabo este estudio con el propósito de buscar alternativas amigables con el ambiente, que hagan parte de un paquete tecnológico eficaz de control. Dos cepas nativas de Psedomonas fluorescens (039T y 021V), provenientes de cultivos de papa, fueron evaluadas contra P. infestans. Las suspensiones bacterianas y los biosurfactantes parcialmente purificados (BPP), producidos por éstas (obtenidos en medio mínimo de sales con querosén), fueron aplicados sobre foliolos desprendidos en ensayos in vitro y experimentos in vivo en plantas de papa, en condiciones controladas en casa de malla. Los resultados demostraron la capacidad que tienen los biosurfactantes y las suspensiones bacterianas para controlar al patógeno, ya que el BPP 039T logró reducir el nivel de severidad de la enfermedad en 79,9% in vitro y 38,5% in vivo, mientras que el BPP 021V redujo en 78,7% in vitro y 30,2% in vivo. Las suspensiones bacterianas redujeron el nivel de severidad en 72,4% (039T) y 66,1% (021V) en las evaluaciones in vitro y 35% en los experimentos in vivo. Los resultados de esta investigación muestran el potencial que tienen los biosurfactantes para el control de la gota en Colombia.</p><p> </p><p><strong>Evaluation of Biosurfactants Produced by Pseudomonas fluorescens for Potato Late Blight Control (Phytophthora infestans (Mont) de Bary) Under Controlled Conditions</strong></p><p>Phytophthora infestans (Mont.) de Bary, causal agent of potato late blight is considered the main limiting pathogen for the production of this crop in Colombia. The usual control of the disease has been performed with systemic fungicides which increase production costs, can induce pathogen resistance and have a negative impact on the environment. Therefore, this study was carried out in order to find effective and environmentally friendly control alternatives for potato late blight. Two Pseudomonas fluorescens native strains (039T and 021V) isolated from potato crops were evaluated against P. infestans. Bacterial suspensions (obtained from minimal salts medium added with kerosene) and partially purified biosurfactants (BPP) were applied on detached leaflets for in vitro assays and on potato plants in greenhouse, for in vivo assays and the measure of inhibitory effect of the disease was assessed. The results showed the ability of P. fluorescens biosurfactants and bacterial suspensions to control the pathogen. BPP 039T was able to reduce the level of severity disease by 79.9% in vitro and 38.5% in vivo, whereas BPP 021V decreased 78.7% in vitro and 30.2% in vivo. Bacterial suspensions reduced the severity level in 72.4% (039T) and 66.1% (021V) in vitro assessments and 35% in the in vivo experiment. These results show the potential of P. fluorescens biosurfactants to control the potato late blight in Colombia.</p>

scholarly journals Field efficacy of mandipropamid for the control of potato late blight

2010 ◽  
Vol 25 (2) ◽  
pp. 157-161
Author(s):  
Emil Rekanovic ◽  
Milos Stepanovic ◽  
Milan Stevic ◽  
Ivana Potocnik ◽  
Biljana Todorovic ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Potato Late Blight ◽  
Field Trials ◽  
High Efficacy ◽  
Highly Effective ◽  
Field Efficacy ◽  
Disease Pressure

The efficacy of mandipropamid (Revus 250 SC) in controlling Phytophthora infestans in potato was evaluated in field trials. The efficacies of Revus 250 SC in comparison with standard fungicides Quadris (azoxystrobin) were tested in several localities in Serbia (Kasarske Livade, Valjevska Kamenica and Opovo) in 2007 and 2008. Both of the tested fungicides exhibited high efficacy in controlling potato late blight. The differencies in the efficacy of Revus 250 SC (96.3- 99.2%) and Quadris (94.1-95.5%) were insignificant. Our experiments showed that the investigated fungicide was highly effective against P. infestans even under high disease pressure.

The molecular mechanisms of Phytophthora infestans in response to reactive oxygen species (ROS) stress

2021 ◽  
Author(s):  
Xiumei Luo ◽  
Tingting Tian ◽  
Maxime Bonnave ◽  
Xue Tan ◽  
Xiaoqing Huang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Molecular Mechanisms ◽  
Reactive Oxygen ◽  
Microbial Pathogens ◽  
Dependent Manner ◽  
Oxygen Species ◽  
Tor Signaling ◽  
Potato Resistance

Reactive oxygen species (ROS) are critical for the growth, development, proliferation, and pathogenicity of microbial pathogens; however, excessive levels of ROS are toxic. Little is known regarding the signaling cascades in response to ROS stress in oomycetes such as Phytophthora infestans, the causal agent of potato late blight. Here, P. infestans was used as a model system to investigate the mechanism underlying the response to ROS stress in oomycete pathogens. Results showed severe defects in sporangium germination, mycelial growth, appressorium formation, and virulence of P. infestans in response to H2O2 stress. Importantly, these phenotypes mimic those of P. infestans treated with rapamycin, the inhibitor of target of rapamycin (TOR, 1-phosphatidylinositol-3-kinase). Strong synergism occurred when P. infestans was treated with a combination of H2O2 and rapamycin, suggesting that a crosstalk exists between ROS stress and the TOR signaling pathway. Comprehensive analysis of transcriptome, proteome and phosphorylation omics showed that H2O2 stress significantly induced the operation of the TOR-mediated autophagy pathway. Monodansylcadaverine (MDC) staining showed that in the presence of H2O2 and rapamycin, the autophagosome level increased in a dosage-dependent manner. Furthermore, transgenic potatoes containing double-stranded RNA of PiTOR (TOR in P. infestans) displayed high resistance to P. infestans. Taken together, TOR is involved in the ROS response and is a potential target for control of oomycete diseases, as host-mediated silencing of PiTOR enhances potato resistance to late blight.

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