scholarly journals Microsatellite primer development for invasive perennial herb, Gypsophila paniculata (Caryophyllaceae)

2018 ◽  
Author(s):  
Hailee B. Leimbach-Maus ◽  
Syndell R. Parks ◽  
Charlyn G. Partridge
Keyword(s):  
Genetic Structure ◽  
Microsatellite Loci ◽  
Perennial Herb ◽  
List Type ◽  
Invasion History ◽  
Sequencing Technology ◽  
Gypsophila Paniculata ◽  
Nuclear Microsatellite ◽  
Nucleotide Repeats ◽  
Herbaceous Perennial

ABSTRACTPremise of the study: Gypsophila paniculata L. (baby’s breath) is an herbaceous perennial that has invaded much of northern and western United States and Canada, outcompeting and crowding out native and endemic species. Microsatellite primers were developed to analyze the genetic structure of invasive populations.Methods and Results: We have identified 16 polymorphic nuclear microsatellite loci for G. paniculata out of 73 loci that successfully amplified from a primer library created using Illumina sequencing technology. The developed primers amplified microsatellite loci in 3 invasive populations in Michigan. Primers amplified di-, tri-, and tetra-nucleotide repeats.Conclusions: These markers will be useful in characterizing the genetic structure of invasive populations throughout North America to aid targeted management efforts, and in native Eurasian populations to better understand invasion history. Five of these developed primers also amplified in G. elegans.

Genetic Structure and Differentiation of Relict Lime Populations Based on the Analysis of Variability of Nuclear Microsatellite Loci

2021 ◽  
Vol 57 (8) ◽  
pp. 920-927
Author(s):  
A. K. Ekart ◽  
A. Ya. Larionova ◽  
A. N. Kravchenko ◽  
S. A. Semerikova ◽  
M. I. Sedaeva
Keyword(s):  
Genetic Structure ◽  
Microsatellite Loci ◽  
Nuclear Microsatellite

The Complex Genetic Structure of Sugarcane Limits Identification of Additional SNP-Defined Simplex Alleles in Microsatellite Loci

2009 ◽  
Vol 2 (3-4) ◽  
pp. 133-142 ◽  
Author(s):  
Cathrine Lynne McIntyre ◽  
Mayling Goode ◽  
Thea Monks ◽  
Graham D. Bonnett
Keyword(s):  
Genetic Structure ◽  
Microsatellite Loci

The spatial genetic structure of bank vole (Myodes glareolus) and yellow-necked mouse (Apodemus flavicollis) populations: The effect of distance and habitat barriers

2009 ◽  
Vol 59 (2) ◽  
pp. 169-187 ◽  
Author(s):  
Michal Kozakiewicz ◽  
Alicja Gryczyńska–Siemiątkowska ◽  
Hanna Panagiotopoulou ◽  
Anna Kozakiewicz ◽  
Robert Rutkowski ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Bank Vole ◽  
Microsatellite Loci ◽  
Myodes Glareolus ◽  
Apodemus Flavicollis ◽  
Island Populations ◽  
Local Populations ◽  
Bank Vole Myodes Glareolus ◽  
Yellow Necked Mouse

AbstractHabitat barriers are considered to be an important factor causing the local reduction of genetic diversity by dividing a population into smaller sections and preventing gene flow between them. However, the “barrier effect” might be different in the case of different species. The effect of geographic distance and water barriers on the genetic structure of populations of two common rodent species – the yellow-necked mouse (Apodemus flavicollis) and the bank vole (Myodes glareolus) living in the area of a lake (on its islands and on two opposite shores) was investigated with the use of microsatellite fragment analysis. The two studied species are characterised by similar habitat requirements, but differ with regard to the socio-spatial structure of the population, individual mobility, capability to cross environmental barriers, and other factors. Trapping was performed for two years in spring and autumn in north-eastern Poland (21°E, 53°N). A total of 160 yellow-necked mouse individuals (7 microsatellite loci) and 346 bank vole individuals (9 microsatellite loci) were analysed. The results of the differentiation analyses (FST and RST) have shown that both the barrier which is formed by a ca. 300 m wide belt of water (between the island and the mainland) and the actual distance of approximately 10 km in continuous populations are sufficient to create genetic differentiation within both species. The differences between local populations living on opposite lake shores are the smallest; differences between any one of them and the island populations are more distinct. All of the genetic diversity indices (the mean number of alleles, mean allelic richness, as well as the observed and expected heterozygosity) of the local populations from the lakeshores were significantly higher than of the small island populations of these two species separated by the water barrier. The more profound “isolation effect” in the case of the island populations of the bank vole, in comparison to the yellow-necked mouse populations, seems to result not only from the lower mobility of the bank vole species, but may also be attributed to other differences in the animals' behaviour.

scholarly journals Body size and clonality consequences for sexual reproduction in a perennial herb of Brazilian rupestrian grasslands

2014 ◽  
Vol 74 (3) ◽  
pp. 744-749 ◽  
Author(s):  
GR Demetrio ◽  
FF Coelho ◽  
MEA Barbosa
Keyword(s):  
Body Size ◽  
Sexual Reproduction ◽  
Life Histories ◽  
Linear Regression Analysis ◽  
Clonal Plants ◽  
Flower Head ◽  
Perennial Herb ◽  
Stressful Environments ◽  
Herbaceous Perennial ◽  
Biology Research

Body size is one of the most important factors regarding herbaceous perennial plants life-histories, and several fitness components of these organisms are related to size. Clonal plants show distinct kinds of reproduction and can develop offspring by sexual or asexual ways. We aimed to understand how body size affects Comanthera nivea (Eriocaulaceae) sexual reproduction and to verify how clonal growth is related to flower head production in this species. We sampled 600 rosettes in rupestrian grasslands and performed linear regression analysis between body size and number of produced flower heads. We also compared the flower head production between isolated rosettes and rosettes within clones. Our results showed that body size was significantly related, but explained only a small part of flower head production. The flower head production was higher in rosettes within clones than in isolated ones. The clones presented a rosette or a small group of rosettes that concentrated the sexual reproduction. Clonality was positively associated with sexual reproduction. Clonality can represent an important way of allowing the persistence of plants by sexual reproduction in markedly seasonal stressful environments. The cases of clonality enhancing the sexual reproduction must be considered and put in focus on reproductive biology research.

scholarly journals Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci

2003 ◽  
Vol 26 (3) ◽  
pp. 355-364 ◽  
Author(s):  
Luciana Rossini Pinto ◽  
Maria Lucia Carneiro Vieira ◽  
Claudio Lopes de Souza Jr. ◽  
Anete Pereira de Souza
Keyword(s):  
Genetic Structure ◽  
Microsatellite Loci ◽  
Recurrent Selection ◽  
Selection Effects ◽  
Reciprocal Recurrent Selection ◽  
Tropical Maize ◽  
Maize Populations
Sign in / Sign up

Export Citation Format

Share Document