scholarly journals Single cell dynamics of embryonic muscle progenitor cells in zebrafish

2018 ◽  
Author(s):  
Priyanka Sharma ◽  
Tyler D. Ruel ◽  
Katrinka M. Kocha ◽  
Shan Liao ◽  
Peng Huang
Keyword(s):  
Single Cell ◽  
Progenitor Cells ◽  
Muscle Injury ◽  
Muscle Growth ◽  
Clonal Analysis ◽  
Muscle Stem Cells ◽  
Injury Repair ◽  
Embryonic Muscle ◽  
Muscle Progenitor Cells

ABSTRACTMuscle stem cells hold a great therapeutic potential in regenerating damaged muscles. However, the in vivo behavior of muscle stem cells during muscle growth and regeneration is still poorly understood. Using zebrafish as a model, we describe the in vivo dynamics and function of dermomyotome cells, a population of embryonic muscle progenitor cells. Dermomyotome cells are located in a superficial layer external to muscle fibers and express many extracellular matrix (ECM) genes including col1a2. Utilizing a new col1a2 transgenic line, we show that dermomyotome cells display a ramified morphology with dynamic cellular processes. Cell lineage tracing demonstrates that col1a2+ dermomyotome cells contribute to normal muscle growth as well as muscle injury repair. Combination of live imaging and single cell clonal analysis reveals a highly-choreographed process of muscle regeneration. Activated dermomyotome cells change from the quiescent ramified morphology to a polarized and elongated morphology and generate daughter cells that fuse with existing muscle fibers. Ablation of the dermomyotome severely compromises muscle injury repair. Our work provides a dynamic view of embryonic muscle progenitor cells during zebrafish muscle regeneration.Summary statementLive imaging and single cell clonal analysis reveal dynamic behaviors of zebrafish embryonic muscle progenitor cells in quiescence and activation.

scholarly journals Single cell dynamics of embryonic muscle progenitor cells in zebrafish

Development ◽  
2019 ◽  
Vol 146 (14) ◽  
pp. dev178400 ◽  
Author(s):  
Priyanka Sharma ◽  
Tyler D. Ruel ◽  
Katrinka M. Kocha ◽  
Shan Liao ◽  
Peng Huang
Keyword(s):  
Single Cell ◽  
Progenitor Cells ◽  
Cell Dynamics ◽  
Embryonic Muscle ◽  
Muscle Progenitor Cells

scholarly journals Quantitative Clonal Analysis and Single-Cell Transcriptomics Reveal Division Kinetics, Hierarchy, and Fate of Oral Epithelial Progenitor Cells

2019 ◽  
Vol 24 (1) ◽  
pp. 183-192.e8 ◽  
Author(s):  
Kyle B. Jones ◽  
Sachiko Furukawa ◽  
Pauline Marangoni ◽  
Hongfang Ma ◽  
Henry Pinkard ◽  
...  
Keyword(s):  
Single Cell ◽  
Progenitor Cells ◽  
Clonal Analysis ◽  
Epithelial Progenitor Cells ◽  
Oral Epithelial

scholarly journals Cell types and clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics

2021 ◽  
Author(s):  
Michael Ratz ◽  
Leonie von Berlin ◽  
Ludvig Larsson ◽  
Marcel Martin ◽  
Jakub Orzechowski Westholm ◽  
...  
Keyword(s):  
Single Cell ◽  
Progenitor Cells ◽  
Mouse Brain ◽  
High Throughput ◽  
Thin Sheet ◽  
Cell Types ◽  
Mammalian Brain ◽  
Fate Mapping ◽  
Cell Phenotypes

SummaryThe mammalian brain contains a large number of specialized cells that develop from a thin sheet of neuroepithelial progenitor cells1,2. Recently, high throughput single-cell technologies have been used to define the molecular diversity of hundreds of cell types in the nervous system3,4. However, the lineage relationships between mature brain cells and progenitor cells are not well understood, because transcriptomic studies do not allow insights into clonal relationships and classical fate-mapping techniques are not scalable5,6. Here we show in vivo barcoding of early progenitor cells that enables simultaneous profiling of cell phenotypes and clonal relations in the mouse brain using single-cell and spatial transcriptomics. We reconstructed thousands of clones to uncover the existence of fate-restricted progenitor cells in the mouse hippocampal neuroepithelium and show that microglia are derived from few primitive myeloid precursors that massively expand to generate widely dispersed progeny. By coupling spatial transcriptomics with clonal barcoding, we disentangle migration patterns of clonally related cells in densely labelled tissue sections. Compared to classical fate mapping, our approach enables high-throughput dense reconstruction of cell phenotypes and clonal relations at the single-cell and tissue level in individual animals and provides an integrated approach for understanding tissue architecture.

scholarly journals Srf controls satellite cell fusion through the maintenance of actin architecture

2017 ◽  
Vol 217 (2) ◽  
pp. 685-700 ◽  
Author(s):  
Voahangy Randrianarison-Huetz ◽  
Aikaterini Papaefthymiou ◽  
Gaëlle Herledan ◽  
Chiara Noviello ◽  
Ulduz Faradova ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Serum Response Factor ◽  
Control Cell ◽  
Muscle Growth ◽  
Muscle Stem Cells ◽  
Hypertrophic Growth ◽  
Proliferation And Differentiation ◽  
Muscle Homeostasis

Satellite cells (SCs) are adult muscle stem cells that are mobilized when muscle homeostasis is perturbed. Here, we show that serum response factor (Srf) is needed for optimal SC-mediated hypertrophic growth. We identified Srf as a master regulator of SC fusion required in both fusion partners, whereas it was dispensable for SC proliferation and differentiation. We show that SC-specific Srf deletion leads to impaired actin cytoskeleton and report the existence of finger-like actin–based protrusions at fusion sites in vertebrates that were notoriously absent in fusion-defective myoblasts lacking Srf. Restoration of a polymerized actin network by overexpression of an α-actin isoform in Srf mutant SCs rescued their fusion with a control cell in vitro and in vivo and reestablished overload-induced muscle growth. These findings demonstrate the importance of Srf in controlling the organization of actin cytoskeleton and actin-based protrusions for myoblast fusion in mammals and its requirement to achieve efficient hypertrophic myofiber growth.

scholarly journals Noggin inactivation affects the number and differentiation potential of muscle progenitor cells in vivo

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Domiziana Costamagna ◽  
Hendrik Mommaerts ◽  
Maurilio Sampaolesi ◽  
Przemko Tylzanowski
Keyword(s):  
Progenitor Cells ◽  
Differentiation Potential ◽  
Muscle Progenitor Cells

scholarly journals Characterization and generation of human definitive multipotent hematopoietic stem/progenitor cells

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Yanling Zhu ◽  
Tianyu Wang ◽  
Jiaming Gu ◽  
Ke Huang ◽  
Tian Zhang ◽  
...  
Keyword(s):  
Single Cell ◽  
Progenitor Cells ◽  
Immune Cells ◽  
Embryonic Stem ◽  
Hematopoietic Stem ◽  
Gene Expression Dynamics ◽  
Firm Basis ◽  
Rock Inhibition ◽  
Definitive Hematopoiesis

AbstractDefinitive hematopoiesis generates hematopoietic stem/progenitor cells (HSPCs) that give rise to all mature blood and immune cells, but remains poorly defined in human. Here, we resolve human hematopoietic populations at the earliest hematopoiesis stage by single-cell RNA-seq. We characterize the distinct molecular profiling between early primitive and definitive hematopoiesis in both human embryonic stem cell (hESC) differentiation and early embryonic development. We identify CD44 to specifically discriminate definitive hematopoiesis and generate definitive HSPCs from hESCs. The multipotency of hESCs-derived HSPCs for various blood and immune cells is validated by single-cell clonal assay. Strikingly, these hESCs-derived HSPCs give rise to blood and lymphoid lineages in vivo. Lastly, we characterize gene-expression dynamics in definitive and primitive hematopoiesis and reveal an unreported role of ROCK-inhibition in enhancing human definitive hematopoiesis. Our study provides a prospect for understanding human early hematopoiesis and a firm basis for generating blood and immune cells for clinical purposes.

scholarly journals In vivo clonal analysis reveals spatiotemporal regulation of thalamic nucleogenesis

2017 ◽  
Author(s):  
Samuel Z.H. Wong ◽  
Earl Parker Scott ◽  
Wenhui Mu ◽  
Xize Guo ◽  
Ella Borgenheimer ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Clonal Analysis ◽  
Cellular Heterogeneity ◽  
Thalamic Nuclei ◽  
Fate Specification ◽  
Cell Divisions ◽  
Spatiotemporal Regulation ◽  
Short Period ◽  
Organizational Principle

ABSTRACTThe thalamus, a crucial regulator of cortical functions, is composed of many nuclei arranged in a spatially complex pattern. Thalamic neurogenesis occurs over a short period during mammalian embryonic development. These features have hampered the effort to understand how regionalization, cell divisions and fate specification are coordinated and produce a wide array of nuclei that exhibit distinct patterns of gene expression and functions. Here, we performed in vivo clonal analysis to track the divisions of individual progenitor cells and spatial allocation of their progeny in the developing mouse thalamus. Quantitative analysis of clone compositions revealed evidence for sequential generation of distinct sets of thalamic nuclei based on the location of the founder progenitor cells. Furthermore, we identified intermediate progenitor cells that produced neurons populating more than one thalamic nuclei, indicating a prolonged specification of nuclear fate. Our study reveals an organizational principle that governs the spatial and temporal progression of cell divisions and fate specification, and provides a framework for studying cellular heterogeneity and connectivity in the mammalian thalamus.

scholarly journals Integrin Profile and In Vivo Homing of Human Smooth Muscle Progenitor Cells

Circulation ◽  
2004 ◽  
Vol 110 (17) ◽  
pp. 2673-2677 ◽  
Author(s):  
Arjun Deb ◽  
Kimberly A. Skelding ◽  
Shaohua Wang ◽  
Margo Reeder ◽  
David Simper ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Progenitor Cells ◽  
Muscle Progenitor Cells
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