scholarly journals Endoreduplication in Drosophila melanogaster progeny after exposure to acute γ-irradiation

2018 ◽  
Author(s):  
Daria A. Skorobagatko ◽  
Alexey A. Mazilov ◽  
Volodymyr Yu. Strashnyuk
Keyword(s):  
Drosophila Melanogaster ◽  
Selection Pressure ◽  
Irradiation Dose ◽  
Γ Irradiation ◽  
Impact Power ◽  
Control Value

AbstractThe purpose of investigation was to study the effect of acute γ-irradiation of parent adults on the endoreduplication in Drosophila melanogaster progeny. As a material used Oregon-R strain. Virgin females and males of Drosophila adults at the age of 3 days were exposed at the doses of 8, 16 and 25 Gy. Giant chromosomes were studied at late 3rd instar larvae by cytomorphometric method. The average polyteny in males increased at the dose of 8 Gy by 10.6%, at the dose of 25 Gy by 7.4%, and did not change at the dose of 16 Gy. In females, the polyteny did not differ from the control value irrespective of the irradiation dose. The statistical impact power of sex on polyteny was 4,9%, the radiation impact was 26,8%. The enhancement of endoruplication is considered as a consequence of an increasing selection pressure after irradiation.

scholarly journals Selection index in Drosophila melanogaster Meig. progeny after exposure to acute γ-irradiation

2019 ◽  
Vol 25 ◽  
pp. 86-91
Author(s):  
D. O. Skorobagatko ◽  
V. Yu. Strashnyuk ◽  
A. A. Mazilov
Keyword(s):  
Drosophila Melanogaster ◽  
Linear Accelerator ◽  
Selection Pressure ◽  
First Generation ◽  
Selection Index ◽  
Control Level ◽  
Wild Type ◽  
Γ Irradiation ◽  
Two Generations ◽  
Selection Indexes

Aim. The purpose of investigation was to study the intensity of selection in two generations of Drosophila melanogaster Meig. after acute g-irradiation. Methods. Experiments were conducted on a wild-type Oregon-R strain. Adult flies in the age of 3 days were irradiated with bremsstrahlung gamma quanta at doses of 8 Gy, 16 Gy and 25 Gy on a linear accelerator of electrons LAE-10. Irradiated (O) and non-irradiated (K) flies were crossed in four different combinations: K×K (control), O×K, K×O, and O×O. The selection index were calculated from the Crow formula based on fertility, mortality/survival in pre-productive period of development. Results. Selection indexes in the generation F1 after irradiation grow in proportion to the dose received: at embryonal stage 2.0–7.2 times, at post-embryonic development – in 1.3–7.6 times. In the generation F2, the indexes of selection were significantly reduced. Conclusions. The selection pressure is substantially increased in the first generation after g-irradiation and weakens, approaching the control level and lower in F2. Keywords: Drosophila melanogaster Meig., fertility, embryonic mortality, pupae mortality, ionizing radiation.

scholarly journals MUTATIONAL ANALYSIS OF THE REGION SURROUNDING THE 93D HEAT SHOCK LOCUS OF DROSOPHILA MELANOGASTER

Genetics ◽  
1984 ◽  
Vol 106 (2) ◽  
pp. 249-265
Author(s):  
Jym Mohler ◽  
Mary Lou Pardue
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Mutational Analysis ◽  
Point Mutations ◽  
Γ Irradiation ◽  
Lethal Mutations ◽  
Complementation Groups ◽  
In Trans ◽  
Shock Locus ◽  
Shock Proteins

ABSTRACT The region containing subdivisions 93C, 93D and 93E on chromosome 3 of Drosophila melanogaster has been screened for visible and lethal mutations. Treatment with three mutagens, γ irradiation, ethyl methanesulfonate and diepoxybutane, has produced mutations that fall into 20 complementation groups, including the previously identified ebony locus. No point mutations affecting the heat shock locus in 93D were detected; however, a pair of deficiencies that overlap in the region of this locus was isolated. Flies heterozygous in trans for this pair of deficiencies are capable of producing all of the major heat shock puffs (except 93D) and the major heat shock proteins. In addition, these flies show recovery of normal protein synthesis following a heat shock.

scholarly journals Chemical and Physical Properties of Cassava Starch–CM-Chitosan–Acrylic Acid Gel Copolymerization by Gamma Irradiation

2014 ◽  
Vol 14 (1) ◽  
pp. 37-42
Author(s):  
Gatot Trimulyadi Rekso
Keyword(s):  
Acrylic Acid ◽  
Irradiation Dose ◽  
Thermo Gravimetric Analysis ◽  
Gravimetric Analysis ◽  
Γ Irradiation ◽  
Equilibrium Degree ◽  
Preparation Conditions ◽  
Gel Fraction ◽  
Gelatinized Starch ◽  
Acid Gel

Starch is a renewable natural polymer that can be decomposed easily in the environment and can be modified to various applications such as biomedical, agricultural and pharmaceutical applications. Copolymerization of gelatinized starch–CM-chitosan and acrylic acid (AAc) in aqueous medium using γ-irradiation was carried out. The preparation conditions, such as irradiation dose and AAc concentration were investigated. The copolymers were characterized by FTIR spectroscopy, thermo-gravimetric analysis (TGA) and scanning electron microscopy (SEM). The results show that by increasing of irradiation dose the gel fraction increases till the dose of 15 kGy. Above the stating dose the gel fraction decreases. The Equilibrium Degree of Swelling (EDS) value slightly increases with increasing irradiation dose and after dose of 15 kGy is decreasing. The swelling of starch–CM-chitosan–AAc hydro gels reduced as the gel content increases. The results indicated that the optimum condition for obtaining hydro gels with desirable properties was irradiated at dose of 15 kGy. The results indicated that SEM revealed that the higher the dose, the lower the copolymer pore size. The starch–CM-chitosan–AAc copolymers have thermal stability higher than that for starch individually.

scholarly journals Endoreduplication in Drosophila melanogaster progeny after exposure to acute γ-irradiation

2020 ◽  
Vol 59 (2) ◽  
pp. 211-220 ◽  
Author(s):  
Daria A. Skorobagatko ◽  
Alexey A. Mazilov ◽  
Volodymyr Yu. Strashnyuk
Keyword(s):  
Drosophila Melanogaster ◽  
Γ Irradiation

Positron Lifetimes and Crystallinity of γ-Irradiated Polypropylenes

2012 ◽  
Vol 733 ◽  
pp. 163-166
Author(s):  
Zhe Chen ◽  
Wei Huang ◽  
Peng Fei Fang ◽  
Wen Yu ◽  
Shao Jie Wang
Keyword(s):  
Differential Scanning Calorimetry ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Positron Lifetime ◽  
Γ Irradiation ◽  
Scanning Calorimetry ◽  
Γ Ray ◽  
Positron Lifetimes ◽  
Dsc Method ◽  
Low Irradiation

The microstructure of the polypropylenes (PP) irradiated by gamma ray was studied by positron lifetime technique and differential scanning calorimetry (DSC). Lifetime measurement shows o-Ps intensity decreases with increasing γ irradiation dose. The crystallinity of irradiated samples was detected by DSC method. The correlation between o-Ps intensity and crystallinity indicates γ-ray can induce higher crystallinity in the polypropylene at low irradiation dose.

Effect of Buffer on the Optical Properties of Irradiated Hydroxyethyl Methacrylate Copolymer

2002 ◽  
Vol 17 (5) ◽  
pp. 1199-1207
Author(s):  
K. F. Chou ◽  
C. C. Han ◽  
Sanboh Lee
Keyword(s):  
Optical Properties ◽  
Irradiation Dose ◽  
Ph Value ◽  
Bathochromic Shift ◽  
Cutoff Wavelength ◽  
Hydroxyethyl Methacrylate ◽  
Γ Irradiation ◽  
Incident Wavelength ◽  
Γ Ray ◽  
Methacrylate Copolymer

The effect of buffer and γ irradiation on the optical properties of hydroxyethyl methacrylate (HEMA) copolymer was investigated. The transmission of HEMA copolymer decreased with the increase of irradiation dose and/or pH value of the buffer. The cutoff wavelength of HEMA copolymer exhibits a bathochromic shift as the γ-ray dose and/or pH value of buffer increases. The influence of atmosphere during γ-ray irradiation on the optical properties of HEMA copolymer was investigated. The change of optical properties of HEMA copolymer irradiated in air was more pronounced than that irradiated in vacuum. Light was scattered by holes in the polymer. The relationship between scattering intensity (Is) and incident wavelength (λ) can be described by the formula Is ∝ λ−n. The span of holes increases with the irradiation dose regardless of radiation atmosphere and pH value in the range of 4.1–6.5. A boundary between the inner and outer layers of HEMA copolymer irradiated in air was observed, separating two differential morphologies of holes.

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