scholarly journals FIFO ATP synthase responds to glycolysis inhibition by localization into the inner boundary membrane

2018 ◽  
Author(s):  
K. Zalyevskiy ◽  
F. Hager ◽  
C. P. Richter ◽  
K. Psathaki ◽  
T. Appelhans ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Significant Proportion ◽  
Tca Cycle ◽  
Longitudinal Axis ◽  
Temporal Organization ◽  
Live Cells ◽  
Mitochondrial Atp Synthase ◽  
Key Enzyme ◽  
Spatio Temporal ◽  
Boundary Membrane

ABSTRACTMitochondrial F1F0ATP synthase is the key enzyme to fuel the cell with essential ATP. Strong indications exist that the respiratory chain and the ATP synthase are physically separated within cristae. How static this organization is, is largely unknown. Here, we investigated the effect of substrate restriction on mitochondrial respiration and the spatio-temporal organization of ATP synthase. By superresolution microscopy, the localization and mobility of single labelled mitochondrial ATP synthase was determined in live cells. We found, that the ATP synthase under oxidative respiration displayed a clear localization and confined mobility in cristae. Trajectories of individual ATP synthase proteins show a perpendicular course to the longitudinal axis of the respective mitochondrion, exactly following the ultrastructure of cristae. When substrate for TCA cycle and respiration was limited, a significant proportion of ATP synthase localized from cristae to the inner boundary membrane, and only less mobile ATP synthase remained in cristae. These observations showing the plasticity of the spatio-temporal organisation of ATP synthase can explain why ATP synthase show interactions with proteins in distinct mitochondrial subcompartments such as inner boundary membrane, cristae junctions and cristae.

scholarly journals The spatio-temporal organization of mitochondrial F1FO ATP synthase in cristae depends on its activity mode

2020 ◽  
Vol 1861 (1) ◽  
pp. 148091 ◽  
Author(s):  
Kirill Salewskij ◽  
Bettina Rieger ◽  
Frances Hager ◽  
Tasnim Arroum ◽  
Patrick Duwe ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Temporal Organization ◽  
F1fo Atp Synthase ◽  
Activity Mode ◽  
Spatio Temporal

scholarly journals G1/S transcription factors assemble in discrete clusters that increase in number as cells grow

2019 ◽  
Author(s):  
Labe Black ◽  
Sylvain Tollis ◽  
Guo Fu ◽  
Jean-Bernard Fiche ◽  
Savanna Dorsey ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Super Resolution ◽  
Temporal Organization ◽  
Stochastic Simulations ◽  
Live Cells ◽  
Number Of Clusters ◽  
Localization Microscopy ◽  
Spatio Temporal ◽  
Cluster Behavior ◽  
Target Promoters

AbstractThe spatio-temporal organization of transcription factor (TF)-promoter interactions is critical for the coordination of transcriptional programs. In budding yeast, the main G1/S transcription factors, SBF and MBF, are limiting with respect to target promoters in small G1 phase cells and accumulate as cells grow, raising the question of how SBF/MBF are dynamically distributed across the G1/S regulon. Super-resolution Photo-Activatable Localization Microscopy (PALM) mapping of the static positions of SBF/MBF subunits revealed that 85% were organized into discrete clusters containing ∼8 copies regardless of cell size, while the number of clusters increased with growth. Stochastic simulations with a mathematical model based on co-localization of promoters in clusters recapitulated observed cluster behavior. A prediction of the model that SBF/MBF should exhibit both fast and slow dynamics was confirmed in PALM experiments on live cells. This spatio-temporal organization of the TFs that activate the G1/S regulon may help coordinate commitment to division.

scholarly journals The Spatio-Temporal Organization of Mitochondrial F1FO-ATP Synthase Is Determined by its Activity and Controlled by IF1

2021 ◽  
Vol 120 (3) ◽  
pp. 193a-194a
Author(s):  
Karin B. Busch ◽  
Kirill Salewskij ◽  
Bettina Rieger ◽  
Verena Weissert ◽  
Frances Hager ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Temporal Organization ◽  
F1fo Atp Synthase ◽  
Spatio Temporal

scholarly journals Nanoscale spatio-temporal diffusion modes measured by simultaneous confocal and STED imaging

2018 ◽  
Author(s):  
Falk Schneider ◽  
Dominic Waithe ◽  
Silvia Galiani ◽  
Jorge Bernadino de la Serna ◽  
Erdinc Sezgin ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Plasma Membranes ◽  
Molecular Diffusion ◽  
Correlation Spectroscopy ◽  
Stimulated Emission ◽  
Temporal Organization ◽  
Live Cells ◽  
Apparent Diffusion Coefficients ◽  
Diffusion Dynamics ◽  
Spatio Temporal

AbstractThe diffusion dynamics in the cellular plasma membrane provides crucial insights into the molecular interactions, organization and bioactivity. Fluorescence correlation spectroscopy combined with super-resolution stimulated emission depletion nanoscopy (STED-FCS) measures such dynamics with high spatial and temporal resolution and reveals nanoscale diffusion characteristics by measuring the molecular diffusion in conventional confocal mode and super-resolved STED mode sequentially. However, to directly link the spatial and the temporal information, a method that simultaneously measures the diffusion in confocal and STED modes is needed. Here, to overcome this problem, we establish an advanced STED-FCS measurement method; line interleaved excitation scanning STED-FCS (LIESS-FCS) which discloses the molecular diffusion modes at different spatial positions with a single measurement. It relies on fast beam-scanning along a line with alternating laser illumination that yields, for each pixel, the apparent diffusion coefficients for two different observation spot sizes (conventional confocal and super-resolved STED). We demonstrate the potential of the LIESS-FCS approach with simulations and experiments on lipid diffusion in model and live cell plasma membranes. We also apply LIESS-FCS to investigate the spatio-temporal organization of GPI-anchored proteins in the plasma membrane of live cells which interestingly show multiple diffusion modes at different spatial positions.

scholarly journals Nuclear Genetic Defects of Mitochondrial ATP Synthase

2014 ◽  
pp. S57-S71 ◽  
Author(s):  
K. HEJZLAROVÁ ◽  
T. MRÁČEK ◽  
M. VRBACKÝ ◽  
V. KAPLANOVÁ ◽  
V. KARBANOVÁ ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Mitochondrial Disease ◽  
Early Onset ◽  
Metabolic Diseases ◽  
The Other ◽  
Genetic Defects ◽  
Biochemical Phenotype ◽  
Mitochondrial Atp Synthase ◽  
Key Enzyme ◽  
Nuclear Mutations

Disorders of ATP synthase, the key enzyme of mitochondrial energy provision belong to the most severe metabolic diseases presenting as early-onset mitochondrial encephalo-cardiomyopathies. Up to now, mutations in four nuclear genes were associated with isolated deficiency of ATP synthase. Two of them, ATP5A1 and ATP5E encode enzyme’s structural subunits α and ε, respectively, while the other two ATPAF2 and TMEM70 encode specific ancillary factors that facilitate the biogenesis of ATP synthase. All these defects share a similar biochemical phenotype with pronounced decrease in the content of fully assembled and functional ATP synthase complex. However, substantial differences can be found in their frequency, molecular mechanism of pathogenesis, clinical manifestation as well as the course of the disease progression. While for TMEM70 the number of reported patients as well as spectrum of the mutations is steadily increasing, mutations in ATP5A1, ATP5E and ATPAF2 genes are very rare. Apparently, TMEM70 gene is highly prone to mutagenesis and this type of a rare mitochondrial disease has a rather frequent incidence. Here we present overview of individual reported cases of nuclear mutations in ATP synthase and discuss, how their analysis can improve our understanding of the enzyme biogenesis.

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