scholarly journals The molecular signatures of compatible and incompatible pollination

2018 ◽  
Author(s):  
Chie Kodera ◽  
Jérémy Just ◽  
Martine Da Rocha ◽  
Antoine Larrieu ◽  
Lucie Riglet ◽  
...  
Keyword(s):  
Global Analysis ◽  
Pollen Grains ◽  
Mitogen Activated Protein Kinase ◽  
Molecular Signatures ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Incompatible Pollination ◽  
Mitogen Activated Protein ◽  
Dependent Cell ◽  
Compatible Pollination

AbstractFertilization in flowering plants depends on the early contact and recognition of pollen grains by the receptive papilla cells of the stigma. To identify the associated molecular pathways, we developed a transcriptomic analysis based on single nucleotide polymorphisms (SNPs) present in twoArabidopsis thalianaaccessions, one used as female and the other as male. We succeeded in distinguishing 80 % of transcripts according to their parental origins and drew up a catalog of genes whose expression is modified after pollen-stigma interaction. Global analysis of our data reveals that pattern-triggered immunity (PTI)-associated transcripts are upregulated after compatible pollination. From our analysis, we predicted the activation of the Mitogen-activated Protein Kinase 3 on the female side after compatible pollination, which we confirmed through expression and mutant analysis. Our work defines the molecular signatures of compatible and incompatible pollination, highlights the active status of incompatible stigmas, and unravels a new MPK3-dependent cell wall feature associated with stigma-pollen interaction.

scholarly journals The molecular signatures of compatible and incompatible pollination in Arabidopsis

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Chie Kodera ◽  
Jérémy Just ◽  
Martine Da Rocha ◽  
Antoine Larrieu ◽  
Lucie Riglet ◽  
...  
Keyword(s):  
Pollen Grains ◽  
Molecular Signatures ◽  
Nucleotide Polymorphisms ◽  
Female Side ◽  
Specific Expression ◽  
Transcriptomic Response ◽  
Single Nucleotide ◽  
Incompatible Pollination ◽  
Important Challenge ◽  
Female Specific

Abstract Background Fertilization in flowering plants depends on the early contact and acceptance of pollen grains by the receptive papilla cells of the stigma. Deciphering the specific transcriptomic response of both pollen and stigmatic cells during their interaction constitutes an important challenge to better our understanding of this cell recognition event. Results Here we describe a transcriptomic analysis based on single nucleotide polymorphisms (SNPs) present in two Arabidopsis thaliana accessions, one used as female and the other as male. This strategy allowed us to distinguish 80% of transcripts according to their parental origins. We also developed a tool which predicts male/female specific expression for genes without SNP. We report an unanticipated transcriptional activity triggered in stigma upon incompatible pollination and show that following compatible interaction, components of the pattern-triggered immunity (PTI) pathway are induced on the female side. Conclusions Our work unveils the molecular signatures of compatible and incompatible pollinations both at the male and female side. We provide invaluable resource and tools to identify potential new molecular players involved in pollen-stigma interaction.

scholarly journals Association of single-nucleotide polymorphisms in <i>NAT9 </i>and <i>MAP3K3</i> genes with litter size traits in Berkshire pigs

2018 ◽  
Vol 61 (4) ◽  
pp. 379-386
Author(s):  
Jung Hye Hwang ◽  
Sang Mi An ◽  
Go Eun Yu ◽  
Da Hye Park ◽  
Deok Gyeong Kang ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Litter Size ◽  
Reproductive Traits ◽  
Mitogen Activated Protein Kinase ◽  
Chromosome 12 ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Pcr Rflp ◽  
Mitogen Activated Protein ◽  
Berkshire Pigs

Abstract. Litter size is an economically important trait in the pig industry. We aimed to identify genetic markers associated with litter size, which can be used in breeding programs for improving reproductive traits. Single-nucleotide polymorphisms (SNPs) of Berkshire pigs in the N-acetyltransferase 9 (NAT9) and Mitogen-activated protein kinase kinase kinase 3 (MAP3K3) genes were from RNA sequencing results, and already exist in the databank (NCBI), and were confirmed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). A total of 272 Berkshire sows were used to examine the genotype, and their association with litter size traits was analyzed. The NAT9 SNP was located in chromosome 12 exon 640 mRNA (A > G) and the MAP3K3 SNP was located in chromosome 12 intron 11 (80, C > T). Association analysis indicated that the GG genotype of NAT9 and the CT genotype of MAP3K3 had the highest values for litter size traits. The GG genotype expressed higher levels of NAT9 mRNA in the endometrium than the other genotypes did, and a positive correlation was found between litter size traits and NAT9, but not MAP3K3 expression level. These results indicate that the NAT9 and MAP3K3 can be used as candidate genes applicable in breeding program for the improvement of litter size traits in Berkshire pigs.

Global analysis of single nucleotide polymorphisms in the exons of human deoxyribonuclease I-like 1 and 2 genes

2010 ◽  
Vol 31 (21) ◽  
pp. 3552-3557 ◽  
Author(s):  
Junko Fujihara ◽  
Toshihiro Yasuda ◽  
Reiko Iida ◽  
Kaori Kimura-Kataoka ◽  
Mikiko Soejima ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Global Analysis ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Deoxyribonuclease I

scholarly journals SHP-2 phosphatase activity is required for PECAM-1-dependent cell motility

2010 ◽  
Vol 299 (4) ◽  
pp. C854-C865 ◽  
Author(s):  
Jing-Xu Zhu ◽  
Gaoyuan Cao ◽  
James T. Williams ◽  
Horace M. DeLisser
Keyword(s):  
Endothelial Cell ◽  
Cell Motility ◽  
Phosphatase Activity ◽  
Tyrosine Phosphorylation ◽  
Mapk Pathway ◽  
Tube Formation ◽  
Mitogen Activated Protein Kinase ◽  
Mitogen Activated Protein ◽  
Catalytically Active ◽  
Dependent Cell

Platelet endothelial cell adhesion molecule-1 (PECAM-1) has been implicated in endothelial cell motility during angiogenesis. Although there is evidence that SHP-2 plays a role in PECAM-1-dependent cell motility, the molecular basis of the activity of SHP-2 in this process has not been defined. To investigate the requirement of SHP-2 in PECAM-1-dependent cell motility, studies were done in which various constructs of SHP-2 were expressed in cell transfectants expressing PECAM-1. We observed that the levels of PECAM-1 tyrosine phosphorylation and SHP-2 association with PECAM-1 were significantly increased in cells expressing a phosphatase-inactive SHP-2 mutant, suggesting that the level of PECAM-1 tyrosine phosphorylation, and thus SHP-2 binding are regulated in part by bound, catalytically active SHP-2. We subsequently found that expression of PECAM-1 stimulated wound-induced migration and the formation of filopodia (a morphological feature of motile cells). These activities were associated with increased mitogen-activated protein kinase (MAPK) activation and the dephosphorylation of paxillin (an event implicated in the activation of MAPK). The phosphatase-inactive SHP-2 mutant, however, suppressed these PECAM-1-dependent phenomena, whereas the activity of PECAM-1 expressing cells was not altered by expression of wild-type SHP-2 or SHP-2 in which the scaffold/adaptor function had been disabled. Pharmacological inhibition of SHP-2 phosphatase activity also suppressed PECAM-1-dependent motility. Furthermore, PECAM-1 expression also stimulates tube formation, but none of the SHP-2 constructs affected this process. These findings therefore suggest a model for the involvement of SHP-2 in PECAM-1-dependent motility in which SHP-2, recruited by its interaction with PECAM-1, targets paxillin to ultimately activate the MAPK pathway and downstream events required for cell motility.

scholarly journals Hyaluronan Activates Cell Motility of v-Src-transformed Cells via Ras-Mitogen–activated Protein Kinase and Phosphoinositide 3-Kinase-Akt in a Tumor-specific Manner

2001 ◽  
Vol 12 (6) ◽  
pp. 1859-1868 ◽  
Author(s):  
Yasuyoshi Sohara ◽  
Naoki Ishiguro ◽  
Kazuya Machida ◽  
Hisashi Kurata ◽  
Aye Aye Thant ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cell Motility ◽  
Mitogen Activated Protein Kinase ◽  
Transformed Cells ◽  
Cell Locomotion ◽  
Specific Manner ◽  
Mitogen Activated Protein ◽  
Dependent Cell ◽  
Phosphoinositide 3 Kinase ◽  
Kinase Pathway

We investigated the production of hyaluronan (HA) and its effect on cell motility in cells expressing the v-src mutants. Transformation of 3Y1 by v-src virtually activated HA secretion, whereas G2A v-src, a nonmyristoylated form of v-src defective in cell transformation, had no effect. In cells expressing the temperature-sensitive mutant of v-Src, HA secretion was temperature dependent. In addition, HA as small as 1 nM, on the other side, activated cell motility in a tumor-specific manner. HA treatment strongly activated the motility of v-Src–transformed 3Y1, whereas it showed no effect on 3Y1- and 3Y1-expressing G2A v-src. HA-dependent cell locomotion was strongly blocked by either expression of dominant-negative Ras or treatment with a Ras farnesyltransferase inhibitor. Similarly, both the MEK1 inhibitor and the kinase inhibitor clearly inhibited HA-dependent cell locomotion. In contrast, cells transformed with an active MEK1 did not respond to the HA. Finally, an anti-CD44–neutralizing antibody could block the activation of cell motility by HA as well as the HA-dependent phosphorylation of mitogen-activated protein kinase and Akt. Taken together, these results suggest that simultaneous activation of the Ras-mitogen-activated protein kinase pathway and the phosphoinositide 3-kinase pathway by the HA-CD44 interaction is required for the activation of HA-dependent cell locomotion in v-Src–transformed cells.

scholarly journals Genetic variation in 1253 immune and inflammation genes and risk of non-Hodgkin lymphoma

Blood ◽  
2007 ◽  
Vol 110 (13) ◽  
pp. 4455-4463 ◽  
Author(s):  
James R. Cerhan ◽  
Stephen M. Ansell ◽  
Zachary S. Fredericksen ◽  
Neil E. Kay ◽  
Mark Liebow ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Hodgkin Lymphoma ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Nucleotide Polymorphisms ◽  
Logistic Regression Models ◽  
Replication Studies ◽  
Non Hodgkin Lymphoma ◽  
Mitogen Activated Protein ◽  
And Migration

Smaller-scale evaluations suggest that common genetic variation in candidate genes related to immune function may predispose to the development of non-Hodgkin lymphoma (NHL). We report an analysis of variants within genes associated with immunity and inflammation and risk of NHL using a panel of 9412 single-nucleotide polymorphisms (SNPs) from 1253 genes in a study of 458 patients with NHL and 484 frequency-matched controls. We modeled haplotypes and risk of NHL, as well as the main effects for all independent SNPs from a gene in multivariate logistic regression models; we separately report results for nonsynonymous (ns) SNPs. In gene-level analyses, the strongest findings (P ≤ .001) were for CREB1, FGG, MAP3K5, RIPK3, LSP1, TRAF1, DUSP2, and ITGB3. In nsSNP analyses, the strongest findings (P ≤ .01) were for ITGB3 L59P (odds ratio [OR] = 0.66; 95% confidence interval [CI] 0.52-0.85), TLR6 V427A (OR = 5.20; CI 1.77-15.3), SELPLG M264V (OR = 3.20; CI 1.48-6.91), UNC84B G671S (OR = 1.50; CI 1.12-2.00), B3GNT3 H328R (OR = 0.74; CI 0.59-0.93), and BAT2 V1883L (OR = 0.64; CI 0.45-0.90). Our results suggest that genetic variation in genes associated with immune response (TRAF1, RIPK3, BAT2, and TLR6), mitogen-activated protein kinase (MAPK) signaling (MAP3K5, DUSP2, and CREB1), lymphocyte trafficking and migration (B3GNT3, SELPLG, and LSP1), and coagulation pathways (FGG and ITGB3) may be important in the etiology of NHL, and should be prioritized in replication studies.

scholarly journals A novel case of an infantile fibrosarcoma-like tumor with KIAA1549-BRAF translocation and an oncogenic NF2p.Q459* SNV with potential clinical significance

2021 ◽  
Vol 5 (1) ◽  
pp. 016-019
Author(s):  
Nagy Anita ◽  
Sergi Consolato M ◽  
de Nanassy Joseph ◽  
Abbot Lesleigh S ◽  
Arnoldo Anthony ◽  
...  
Keyword(s):  
Clinical Significance ◽  
Fusion Gene ◽  
Mapk Pathway ◽  
Mitogen Activated Protein Kinase ◽  
Single Nucleotide ◽  
Infantile Fibrosarcoma ◽  
Congenital Mesoblastic Nephroma ◽  
Mitogen Activated Protein ◽  
Infant Girl ◽  
Pan Cancer

We report a case of a right gluteal mass from the sacroiliac joint to the knee of an infant girl. Biopsy showed histopathological features similar to infantile fibrosarcoma (IFS). However, unlike most IFS, no ETV6-NTRK3 fusion gene abnormality was detected. Molecular analysis with TruSight RNA Pan-Cancer Panel detected the presence of KIAA1549-BRAF translocation and an oncogenic NF2p.Q459* SNV with potential clinical significance. A review revealed that the combination of this patient’s tumor site with the presence of a KIAA1549-BRAF translocation abnormality and an accompanying single nucleotide variant has not been previously described. The detection of this translocation abnormality raises the possibility that the spindle cell tumors in infants with an absence of the ETV6-NTRK3 fusion gene abnormality might have a distinct pathogenetic mechanism different from the previously known IFS and congenital mesoblastic nephroma. Furthermore, the discovery of BRAF translocation and its aberrant signaling of the mitogen-activated protein kinase (MAPK) pathway in this tumor contributes to the promise of clinical benefit of using the MEKi trametinib for the treatment of progressive disease that is refractory to conventional chemotherapy.

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