scholarly journals Anticlastogenic Effect of Ulva Fasciata, Against Cyclophosphamide and Mitomycin C Induced Chromosomal Damage in Swiss Albino Mice

2018 ◽  
Author(s):  
Jayashree Dolpady ◽  
K.K. Vijayalaxmi
Keyword(s):  
Mitomycin C ◽  
Chromosomal Aberrations ◽  
Human Cancer ◽  
Ethanol Extract ◽  
Biologically Active ◽  
Chromosomal Damage ◽  
Oral Gavage ◽  
Ulva Fasciata ◽  
Green Seaweed ◽  
Anticlastogenic Effect

ABSTRACTChemoprevention is a strategy to reduce the incidence of human cancer either by inhibiting initiation of carcinogenesis or by preventing exposure to carcinogens, by the use of plant or animal derived ingredients. In the present study we investigated the anticlastogenic effect of ethanol extract of Ulva fasciata, a green seaweed, against the chromosomal aberration and micronucleus induced by the anticancer drugs cyclophosphamide and mitomycin C. Three doses of extract (25, 50 and 75 mg/kg.b.w) was given by oral gavage for 5 days at 24 hr. intervals and on the 5th day, CP (25 and 50mg/kg.b.w) or MMC (1 and 2mg/kg.b.w) were intraperitoneally injected and 24hrs. later micronucleus and chromosomal aberrations assays were performed. Our results show that Ulva extract gave significant protection against the CP and MMC induced damages by reducing micronucleus and chromosomal aberrations. The protection imparted by Ulva could be due to the synergistic and/or additive effects of biologically active ingredients present in the seaweed.

A food ingredient based on green seaweed in the composition of bakery products. Risks and potential

2021 ◽  
Vol 30 (6) ◽  
pp. 39-43
Author(s):  
V.A. Lyakh ◽  
◽  
L.N. Fedyanina ◽  
E.S. Smertina ◽  
A.A. Shamanskaya ◽  
...  
Keyword(s):  
Russian Federation ◽  
Ethanol Extract ◽  
Biologically Active ◽  
Wheat Bread ◽  
Bakery Products ◽  
Food Ingredient ◽  
Green Seaweed ◽  
The Russian Federation ◽  
Processed Products

The paper presents the results of the development wheat bread using a new ingredient – a water-ethanol extract of the sea green alga Ulva lactuca, which contains biologically active substances, minerals, trace elements, and has a proven positive effect on the human body. Rational dosages of the extract have been selected, eliminating the technological risks of its use in the recipe for bakery products. The safety and quality of a new food ingredient and bakery products with its addition is shown in accordance with the requirements of the regulatory documents of the Russian Federation and the EAEU. This work was supported by the Council for Grants of the President of the Russian Federation for State support of young Russian scientists. Grant number is MK-3686.2021.4. The topic of the scientific research is «Rational use of processed products of non-fish objects of water industry in the technology of safe and high-quality food»

Ecological and physiological as well as biochemical properties of representatives of the Genus sedum L.

2014 ◽  
Vol 25 (3-4) ◽  
pp. 24-33
Author(s):  
O. I. Dzjuba ◽  
M. V. Yatsenko
Keyword(s):  
Nitric Oxide ◽  
Cell Line ◽  
Methanol Extract ◽  
Hepatoma Cell ◽  
Ethanol Extract ◽  
Biochemical Properties ◽  
Biologically Active ◽  
Hepatoma Cell Line ◽  
Dependent Manner ◽  
Dose Dependent

The article deals with the history of the study and the current state of research of physiological and biochemical properties of the plant genus Sedum that are useful for human and has been used in folk medicine for many years. It was noticed that antioxidant properties of extracts from plants S. sarmentosum, S. sempervivoides, S. takesimense were caused by the presence of phenolic compounds. Methanol extract of plants S. takesimense exhibited strong scavenging activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals as well as significant inhibitory effects on lipid peroxidation and low density lipoprotein (LDL) oxidation induced by a metal ion Cu2+. Various immunomodulatory activities of various fractions of plants extracts (S. dendroideum, S. kamtschaticum, S. sarmentosum, S. telephium) are observed. It was shown that the ethanol extract of S. sarmentosum and it’s fractions suppressed specific antibody and cellular responses to ovalbumin in mice. The methanol extract of plants S. sarmentosum reduced the levels of anti-inflammatory markers, such as volume of exudates, number of polymorphonuclear leukocytes, suppressed nitric oxide synthesis in activated macrophages via suppressed induction of inducible nitric oxide synthase (iNOS). Polysaccharides fractions from plants S. telephium inducing productions of tumor necrosis factor alpha (TNF-α), increasing the intensity of phagocytosis in vitro and in vivo. Methanol extract from the whole part of S. kamtschaticum strongly inhibit PGE2 production from lipopolysaccharide-induced RAW 264.7 cells, a mouse macrophage cell line via modulating activity in gene expression of the enzyme cyclooxygenase-2 (COX-2). The methanol extract of plants S. sarmentosum and the major kaempferol glycosides from S. dendroideum have antinociceptive activity. It was noticed that anti-adipogenic activity of extracts from plants S. kamtschaticum were caused by inhibition of peroxisome-proliferator-activated receptor γ (PPARγ) expression and it’s dependent target genes, such as genes encoding adipocyte protein 2 (аР2), lipoprotein lipase (LPL), adiponectin and CD36. Polysaccharides fractions from S. telephium cause inhibition of cell adhesion of human fibroblast (MRC5) to laminin and fibronectin via interfere with integrin-mediated cell behaviour and they contributed to the role of polysaccharides in cell-matrix interaction. The methanol extract of plants S. sarmentosum exhibited a significant inhibitory activity in the chick embryo chorioallantoic membrane angiogenesis in a dose-dependent manner. The crude alkaloid fraction of S. sarmentosum caused a dose-dependent inhibition of cell proliferation on murine hepatoma cell line BNL CL.2 and human hepatoma cell line HepG2 without necrosis or apoptosis. Alkaloids from plants S. sarmentosum may improve survival of hepatoma patients via the inhibition of excessive growth of tumor cells. Plant’s juices have antiviral activity (S. sarmentosum, S. spurium, S. stahlii). Crude ethanol extract S. praealtum have spermicidal activity of the in mice and a relevant inhibitory effect of aqueous extract on human spermatozoa motility as well as an anti-fertilizing activity in rats. Hepatoprotective triterpenes, e.g., δ-amyrone, 3-epi-δ-amyrin, δ-amyrin and sarmentolin were isolated from S. sarmentosum. 2- and 2,6-substituted piperidine alkaloids (e.g., norsedamine, allosedridine, sedamine, allosedamine) are observed in plants S. acre, which in the presence of data on the use of pyridine and piperidine derivatives for treating neurodegenerative diseases (e.g., Alzheimer's disease), points on the promising research in this area. Taking into account that biologically active compounds are accumulated in the aboveground vegetative organs of plants of Sedum, the prospects of further study of the use of Sedum for the purposes of biotechnology and in the pharmaceutical industry becomes apparent. This work extends the existing views regarding the use of plants Sedum.

Determination of nutritional and functional principles of the jujube pulp fruit, ecotype Zfisef from northeastern of Algeria

2015 ◽  
Vol 5 (3) ◽  
pp. 84-93
Author(s):  
Insaf Fatima Zohra Mansour ◽  
Mokhtaria Yasmina Boufadi ◽  
Fatima Zohra Elkadi ◽  
Amina Imène Benali ◽  
Mohammed Benali
Keyword(s):  
Antioxidant Activity ◽  
Iron Reduction ◽  
Ethanol Extract ◽  
Matter Content ◽  
Biologically Active ◽  
Dry Matter Content ◽  
Fruit Pulp ◽  
Total Carotenoids ◽  
Jujube Fruit ◽  
Total Anthocyanins

  The aim of the present work was to valorize the exotic fruit of jujube, Zfisef ecotype, growing in the western region of Algeria and highly appreciated by the population. The various analyzes of primary metabolites showed an interesting nutritional profile. The fruit pulp of jujube had a dry matter content of 81±0.21%. The amount of ash (6.1±0.16%) was high and demonstrated a wealth of minerals. Total sugars represented more than 23.20±1.85% while proteins and lipids represented only 2.88±0.32 SAB equivalent and 0.28±0.03% respectively. Vitamin C from the pulp of the fruit of the jujube tree was 194±4.36 mg/100 g of substrate and appeared significant compared to other varieties of other biotope and to fruit ripening stages. The fruit pulp of jujube (50g) gave an ethanol extract of 3.78±0.31%. The latter allowed us to determine secondary metabolites, in this case the total polyphenols (7.80±0.40 mg EAG/g) flavonoids (25.58±1.96 mg EQ/g) gallic tannins (0.15±0.025 μg EAG/100g) catechin tannins (3.48±0.28 μg ECT/mg) total anthocyanins (4.81±0.44 mg ECy3g/100g) and total carotenoids (5.02±0.19 mg E b-carotene/100g). These biologically active molecules conferred to the product good DPPH antioxidant activity (56.60±3.99% with PFJ extract concentration of 0.25±0.062 mg/ml). Concentration of product required for 50% inhibition was 0.22mg/ml. Iron reduction power of our jujube fruit extract exhibited a good antioxidant activity compared to ascorbic acid. Also the presence of many phenolic compounds determined by LC-MS/MS with var-ied identities and antioxidant characters generate interest in making this product a functional food.

scholarly journals Bifunctional Phenol Quinone Methide Precursors: Synthesis and Biological Activity

2019 ◽  
Vol 92 (1) ◽  
pp. 29-41 ◽  
Author(s):  
Matija Sambol ◽  
Katja Ester ◽  
Antonija Husak ◽  
Đani Škalamera ◽  
Ivo Piantanida ◽  
...  
Keyword(s):  
Biological Activity ◽  
Rational Design ◽  
Human Cancer ◽  
Conclusive Evidence ◽  
Biologically Active ◽  
Quinone Methide ◽  
Breast Adenocarcinoma ◽  
Cross Link ◽  
Human Cancer Cell Lines ◽  
Sequential Generation

New bifunctional quinone methide (QM) precursors, bisphenols 2a–2e, and monofunctional QM precursor 7 were synthesized. Upon treatment with fluoride, desilylation triggers formation of reactive intermediates, QMs, which was demonstrated by trapping QM with azide or methanol. The ability of QMs to alkylate and cross-link DNA was assayed by investigation of the effects of QMs to DNA denaturing, but without conclusive evidence. Furthermore, treatment of a plasmid DNA with compounds 2a–2e and KF, followed by the analysis by alkaline denaturing gel electrophoresis, did not provide evidence for the DNA cross-linking. MTT test performed on two human cancer cell lines (MCF7 breast adenocarcinoma and SUM159 pleomorphic breast carcinoma), with and without fluoride, indicated that 2a–2e or the corresponding QMs did not exhibit cytotoxic activity, in line with the lack of ability to cross-link DNA. The lack of reactivity with DNA and biological activity were explained by sequential formation of QMs where bifunctional cytotoxic reagent is probably never produced. Instead, the sequential generation of monofunctional QM followed by a faster hydrolysis leads to the destruction of biologically active reagent. The findings described here are particularly important for the rational design of new generation of QM precursor molecules that will attain desirable DNA reactivity and cytotoxicity.

scholarly journals Phytochemical Characterization of an Adaptogenic Preparation from Rhodiola heterodonta

2009 ◽  
Vol 4 (8) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Mary H. Grace ◽  
Gad G. Yousef ◽  
Anvar G. Kurmukov ◽  
Ilya Raskin ◽  
Mary Ann Lila
Keyword(s):  
Methyl Ether ◽  
Epigallocatechin Gallate ◽  
Ethanol Extract ◽  
Biologically Active ◽  
Etoh Extract ◽  
Phytochemical Constituents ◽  
First Time ◽  
Adaptogenic Activity ◽  
Oligomeric Compounds

The phytochemical constituents of a biologically active, standardized, 80% ethanol extract of Rhodiola heterodonta were characterized. The extract was fractionated over a Sephadex LH-20 column to afford two main fractions representing two classes of secondary metabolites: phenylethanoids and proanthocyanidins. This fractionation facilitated the identification and quantification of individual compounds in the fractions and sub-fractions using HPLC, and LC-MS. The major compounds in the phenylethanoid fraction were heterodontoside, tyrosol methyl ether, salidroside, viridoside, mongrhoside, tyrosol, and the cyanogenic glucoside rhodiocyanoside A. These seven compounds comprised 17.4% of the EtOH extract. Proanthocyanidins ranged from oligomers to polymers based on epigallocatechin and gallate units. The main identified oligomeric compounds in the proanthocyanidin fraction were epigallocatechin gallate, epigallocatechin-epigallocatechin-3-O-gallate and 3-O-galloyl-epigallocatechin-epigallocatechin-3-O-gallate, which constituted 1.75% of the ethanol extract. Tyrosol methyl ether, mongrhoside, and the two proanthocyanidin dimers were reported for the first time from this species in this study. Intra-peritoneal injection of the 80% ethanol extract increased survival time of mice under hypoxia by 192%, as an indication of adaptogenic activity.

scholarly journals Modulation of CAT-2B-Mediated l-Arginine Uptake and Nitric Oxide Biosynthesis in HCT116 Cell Line Through Biological Activity of 4′-Geranyloxyferulic Acid Extract from Quinoa Seeds

2019 ◽  
Vol 20 (13) ◽  
pp. 3262
Author(s):  
Sara Franceschelli ◽  
Daniela Maria Pia Gatta ◽  
Mirko Pesce ◽  
Alessio Ferrone ◽  
José Luis Quiles ◽  
...  
Keyword(s):  
Cell Line ◽  
Cancer Progression ◽  
Hct116 Cell ◽  
Chenopodium Quinoa ◽  
Ethanol Extract ◽  
Biologically Active ◽  
Inflammatory Status ◽  
Arginine Uptake ◽  
Cellular Environment ◽  
Hct 116

Chenopodium quinoa Wild is a “pseudocereal” grain which attracts a lot of attention in the scientific community as it has a positive effect on health. Here, we investigate the presence of biologically active O-prenylated phenylpropanoids in the ethanol extract of commercially available quinoa seeds. We claim that 4′-Geranyloxyferulic acid (GOFA) was the only phytochemical product found that belongs to quinoa’s group secondary metabolites. We studied the changes in the oxidative and inflammatory status of the cellular environment in HCT 116 cell line processed with quinoa extract and its component GOFA; the implementation was done through the analysis of the antioxidant enzymes (SOD and CAT), the pro-inflammatory components (iNOS, IL-6 and TNF-α), and the products of intermediary metabolism (ONOO−, O2−). Moreover, the l-arginine uptake was proposed as a target of the tested compounds. We demonstrated that the GOFA, through a decrease of the CAT-2B expression, leads to a reduction of the l-arginine uptake, downregulating the harmful iNOS and restoring the altered redox state. These results propose a new molecular target involved in the reduction of the critical inflammatory process responsible for the cancer progression.

Hydroxyurea induces chromosomal damage in G2 and enhances the clastogenic effect of mitomycin C in Fanconi anemia cells

2015 ◽  
Vol 56 (5) ◽  
pp. 457-467 ◽  
Author(s):  
Bertha Molina ◽  
Francesco Marchetti ◽  
Laura Gómez ◽  
Sandra Ramos ◽  
Leda Torres ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Fanconi Anemia ◽  
Chromosomal Damage ◽  
Clastogenic Effect

scholarly journals The efficiency of propolis in post-colostral dairy calves

2012 ◽  
Vol 55 (4) ◽  
pp. 315-324 ◽  
Author(s):  
R. Kupczyński ◽  
M. Adamski ◽  
D. Falta ◽  
A. Roman
Keyword(s):  
Health Status ◽  
Neonatal Period ◽  
Clinical Symptoms ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Influence ◽  
Biologically Active ◽  
Dairy Calves ◽  
Haematological Parameters ◽  
Fe Content

Abstract. The study aimed at an assessment of a possibility of calves’ health status improvement in neonatal period by preventive application of 10 % ethanol extract of propolis (EEP). An influence of EEP on selected biochemical and haematological parameters of blood, body weight gains and diarrhoea symptoms intensity was determined. Propolis contains a range of biologically active compounds and exhibits numerous beneficial properties. Ethanolic extract is a form of propolis that is usually used in prevention. Forty five calves were used in the experiment. The assessment of clinical symptoms of diarrhoea, dehydration and vitality was conducted and calves (without symptoms of diarrhoea) were divided into 3 groups (15 calves in each): control, and two experimental (2 and 4 ml of EEP/day). The results of the study point that EEP may be a useful mean improving health status of calves. After an application of propolis in a dose of 4 ml/day higher daily gains were noted when compared to the control calves. Although no obvious influence of EEP on haematological parameters was noted, the positive influence on erythropoiesis and Fe content was observed. Higher EEP dose caused a significant decrease in lactic acid (LA) level. No influence on macroelements and electrolytes in blood serum was noted.

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