scholarly journals Kilohertz frame-rate two-photon tomography

2018 ◽  
Author(s):  
Abbas Kazemipour ◽  
Ondrej Novak ◽  
Daniel Flickinger ◽  
Jonathan S. Marvin ◽  
Jonathan King ◽  
...  
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Glutamate Release ◽  
Single Particle Tracking ◽  
Frame Rate ◽  
Mammalian Brain ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Calcium Sensors ◽  
Structural Image

SummaryPoint-scanning two-photon microscopy enables high-resolution imaging within scattering specimens such as the mammalian brain, but sequential acquisition of voxels fundamentally limits imaging speed. We developed a two-photon imaging technique that scans lines of excitation across a focal plane at multiple angles and uses prior information to recover high-resolution images at over 1.4 billion voxels per second. Using a structural image as a prior for recording neural activity, we imaged visually-evoked and spontaneous glutamate release across hundreds of dendritic spines in mice at depths over 250 µm and frame-rates over 1 kHz. Dendritic glutamate transients in anaesthetized mice are synchronized within spatially-contiguous domains spanning tens of microns at frequencies ranging from 1-100 Hz. We demonstrate high-speed recording of acetylcholine and calcium sensors, 3D single-particle tracking, and imaging in densely-labeled cortex. Our method surpasses limits on the speed of raster-scanned imaging imposed by fluorescence lifetime.

scholarly journals The Power of Single and Multibeam Two-Photon Microscopy for High-Resolution and High-Speed Deep Tissue and Intravital Imaging

2007 ◽  
Vol 93 (7) ◽  
pp. 2519-2529 ◽  
Author(s):  
Raluca Niesner ◽  
Volker Andresen ◽  
Jens Neumann ◽  
Heinrich Spiecker ◽  
Matthias Gunzer
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Intravital Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Bridging scales in scattering tissues via multifocal two-photon microscopy

2020 ◽  
Author(s):  
David Chen ◽  
Fabian Segovia-Miranda ◽  
Noreen Walker ◽  
Jose I. Valenzuela ◽  
Marino Zerial ◽  
...  
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Large Scale ◽  
Fluorescent Proteins ◽  
Morphological Changes ◽  
Optical Techniques ◽  
Tissue Clearing ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Subcellular Resolution

Imaging biological systems at subcellular resolution and across scales is essential to under-standing how cells form tissues, organs, and organisms. However, existing large-scale optical techniques often require harsh tissue-clearing methods that cause significant morphological changes, compromise the integrity of cell membranes, and reduce the signal of fluorescent proteins. Here, we demonstrate multifocal two-photon microscopy that enables imaging mesoscopic scattering samples in their native tissue environment at high resolution and high speed.

scholarly journals Fast Objective Coupled Planar Illumination Microscopy

2018 ◽  
Author(s):  
Cody Greer ◽  
Timothy E. Holy
Keyword(s):  
Fluorescence Microscopy ◽  
High Speed ◽  
Imaging Techniques ◽  
Light Sheet ◽  
Frame Rate ◽  
Three Dimensions ◽  
Two Photon Microscopy ◽  
Image Sharing ◽  
Scanning Rate ◽  
Fast Light

Among optical imaging techniques light sheet fluorescence microscopy stands out as one of the most attractive for capturing high-speed biological dynamics unfolding in three dimensions. The technique is potentially millions of times faster than point-scanning techniques such as two-photon microscopy. However current-generation light sheet microscopes are limited by volume scanning rate and/or camera frame rate. We present speed-optimized Objective Coupled Planar Illumination (OCPI) microscopy, a fast light sheet technique that avoids compromising image quality or photon efficiency. We increase volume scanning rate to 40 Hz for volumes up to 700 µm thick and introduce Multi-Camera Image Sharing (MCIS), a technique to scale imaging rate by parallelizing acquisition across cameras. Finally, we demonstrate fast calcium imaging of the larval zebrafish brain and find a heartbeat-induced artifact that can be removed by filtering when the imaging rate exceeds 15 Hz. These advances extend the reach of fluorescence microscopy for monitoring fast processes in large volumes.

scholarly journals Phaseless Terahertz Coded-Aperture Imaging Based on Incoherent Detection

Sensors ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 226 ◽  
Author(s):  
Long Peng ◽  
Chenggao Luo ◽  
Bin Deng ◽  
Hongqiang Wang ◽  
Yuliang Qin ◽  
...  
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Phase Retrieval ◽  
Time Frame ◽  
Frame Rate ◽  
Future Research ◽  
Coded Aperture ◽  
Main Work ◽  
Coded Aperture Imaging ◽  
The Matrix

In this paper, we propose a phaseless terahertz coded-aperture imaging (PTCAI) method by using a single incoherent detector or an incoherent detection array. We at first analyze and model the system architecture, derive the matrix imaging equation, and then study the phase retrieval techniques to reconstruct the original target with high resolution. Numerical experiments are performed and the results show that the proposed method can significantly reduce the system complexity in the receiving process while maintaining high resolution imaging capability. Furthermore, the approach of using incoherent detection array instead of single detector is capable of decreasing the encoding and sampling times, and therefore helps to improve the imaging frame rate. In our future research, the method proposed in this paper will be experimentally tested and validated, and high-speed PTCAI at nearly real-time frame rates will be the main work.

Ultrafast two-photon microscopy for high-speed brain imaging in awake mice (Conference Presentation)

2018 ◽  
Author(s):  
Radoslaw Chrapkiewicz ◽  
Tong Zhang ◽  
Oscar Hernandez ◽  
Adam S. Shai ◽  
Mark J. Wagner ◽  
...  
Keyword(s):  
Brain Imaging ◽  
High Speed ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals A platform for brain-wide imaging and reconstruction of individual neurons

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Michael N Economo ◽  
Nathan G Clack ◽  
Luke D Lavis ◽  
Charles R Gerfen ◽  
Karel Svoboda ◽  
...  
Keyword(s):  
Long Range ◽  
High Speed ◽  
Large Scale ◽  
Three Dimensional ◽  
Image Data ◽  
Mammalian Brain ◽  
Projection Neurons ◽  
Computational Tools ◽  
Two Photon ◽  
Axonal Arbors

The structure of axonal arbors controls how signals from individual neurons are routed within the mammalian brain. However, the arbors of very few long-range projection neurons have been reconstructed in their entirety, as axons with diameters as small as 100 nm arborize in target regions dispersed over many millimeters of tissue. We introduce a platform for high-resolution, three-dimensional fluorescence imaging of complete tissue volumes that enables the visualization and reconstruction of long-range axonal arbors. This platform relies on a high-speed two-photon microscope integrated with a tissue vibratome and a suite of computational tools for large-scale image data. We demonstrate the power of this approach by reconstructing the axonal arbors of multiple neurons in the motor cortex across a single mouse brain.

scholarly journals High speed functional imaging with source localized multifocal two-photon microscopy

2018 ◽  
Vol 9 (8) ◽  
pp. 3678 ◽  
Author(s):  
Peter Quicke ◽  
Stephanie Reynolds ◽  
Mark Neil ◽  
Thomas Knöpfel ◽  
Simon R. Schultz ◽  
...  
Keyword(s):  
Functional Imaging ◽  
High Speed ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Real-time high-resolution video stabilization using high-frame-rate jitter sensing

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Sushil Raut ◽  
Kohei Shimasaki ◽  
Sanjay Singh ◽  
Takeshi Takaki ◽  
Idaku Ishii
Keyword(s):  
High Resolution ◽  
Real Time ◽  
High Speed ◽  
Image Sequences ◽  
Frame Rate ◽  
Video Stabilization ◽  
Feature Points ◽  
Cmos Camera ◽  
Camera System ◽  
Digital Video Stabilization

AbstractIn this study, the novel approach of real-time video stabilization system using a high-frame-rate (HFR) jitter sensing device is demonstrated to realize the computationally efficient technique of digital video stabilization for high-resolution image sequences. This system consists of a high-speed camera to extract and track feature points in gray-level $$512\times 496$$512×496 image sequences at 1000 fps and a high-resolution CMOS camera to capture $$2048\times 2048$$2048×2048 image sequences considering their hybridization to achieve real-time stabilization. The high-speed camera functions as a real-time HFR jitter sensing device to measure an apparent jitter movement of the system by considering two ways of computational acceleration; (1) feature point extraction with a parallel processing circuit module of the Harris corner detection and (2) corresponding hundreds of feature points at the current frame to those in the neighbor ranges at the previous frame on the assumption of small frame-to-frame displacement in high-speed vision. The proposed hybrid-camera system can digitally stabilize the $$2048\times 2048$$2048×2048 images captured with the high-resolution CMOS camera by compensating the sensed jitter-displacement in real time for displaying to human eyes on a computer display. The experiments were conducted to demonstrate the effectiveness of hybrid-camera-based digital video stabilization such as (a) verification when the hybrid-camera system in the pan direction in front of a checkered pattern, (b) stabilization in video shooting a photographic pattern when the system moved with a mixed-displacement motion of jitter and constant low-velocity in the pan direction, and (c) stabilization in video shooting a real-world outdoor scene when an operator holding hand-held hybrid-camera module while walking on the stairs.

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