scholarly journals Experimental infection characteristics ofBordetellapertussis via aerosol challenge on rhesus macaques

2018 ◽  
Author(s):  
Dachao Mou ◽  
Peng Luo ◽  
Jiangli Liang ◽  
Qiuyan Ji ◽  
Lichan Wang ◽  
...  
Keyword(s):  
Rhesus Macaque ◽  
Rhesus Macaques ◽  
Seroconversion Rate ◽  
Geometric Mean ◽  
Human Beings ◽  
Pertussis Infection ◽  
Cell Counts ◽  
Baseline Levels ◽  
Group 2 ◽  
Infant Rhesus

AbstractThe effect of aerosol challenge of rhesus macaques withBordetella pertussisand the feasibility of using rhesus monkeys as an animal model for pertussis infection were evaluated in this study. Four 1-year old rhesus macaques were aerosol challenged withB. pertussisat the concentration of 105CFU/mL for 30 min (group 1) or 60 min (group 2). Rectal temperature was found slightly increased at days 3 and 5 and returned to baseline levels at day 21 after challenge. White blood cell counts peaked at day 7, with a 4.7~6.1-fold increase and returned to baseline levels at day 45. Bacteria colonization of nasopharyngeal swabs was observed, and the number of colonies was gradually increased and peaked at day 14, reaching 5.4-8.1 × 106/mL. The seroconversion rate of anti-pertussis toxin (PT), pertactin (PRN), and filamentous hemagglutinin(FHA) antibodies was 100%, with an increase in geometric mean titers after challenge. Analysis of cytokines revealed that the levels of cytokines including IL-2, IL-6, IL-8, IL-10, IL-17A, IL-13, IL-12, and IL-18 were significantly increased at days 5 to 14 in group 2. These results demonstrate that the characteristic of pertussis infection in infant rhesus macaque was similar as in human beings, which provide a clue to using infant rhesus macaque as a candidate model for pertussis infection in the future studies

scholarly journals Phase I Evaluation of ΔvirG Shigella sonnei Live, Attenuated, Oral Vaccine Strain WRSS1 in Healthy Adults

2002 ◽  
Vol 70 (4) ◽  
pp. 2016-2021 ◽  
Author(s):  
Karen L. Kotloff ◽  
David N. Taylor ◽  
Marcelo B. Sztein ◽  
Steven S. Wasserman ◽  
Genevieve A. Losonsky ◽  
...  
Keyword(s):  
Phase I ◽  
Oral Vaccine ◽  
Geometric Mean ◽  
Healthy Adults ◽  
Shigella Sonnei ◽  
Low Grade ◽  
Double Blind ◽  
Antibody Secreting Cell ◽  
Cell Counts ◽  
Group 2

ABSTRACT We conducted a phase I trial with healthy adults to evaluate WRSS1, a live, oral ΔvirG Shigella sonnei vaccine candidate. In a double-blind, randomized, dose-escalating fashion, inpatient volunteers received a single dose of either placebo (n = 7) or vaccine (n = 27) at 3 × 103 CFU (group 1), 3 × 104 CFU (group 2), 3 × 105 CFU (group 3), or 3 × 106 CFU (group 4). The vaccine was generally well tolerated, although a low-grade fever or mild diarrhea occurred in six (22%) of the vaccine recipients. WRSS1 was recovered from the stools of 50 to 100% of the vaccinees in each group. The geometric mean peak anti-lipopolysaccharide responses in groups 1 to 4, respectively, were 99, 39, 278, and 233 for immunoglobulin (IgA) antibody-secreting cell counts; 401, 201, 533, and 284 for serum reciprocal IgG titers; and 25, 3, 489, and 1,092 for fecal IgA reciprocal titers. Postvaccination increases in gamma interferon production in response to Shigella antigens occurred in some volunteers. We conclude that WRSS1 vaccine is remarkably immunogenic in doses ranging from 103 to 106 CFU but elicits clinical reactions that must be assessed in further volunteer trials.

scholarly journals Infant Rhesus Macaques as a Nonhuman Primate Model of Bordetella Pertussis

2020 ◽  
Author(s):  
Li Shi ◽  
Wenwen Jiang ◽  
Chen Wei ◽  
Dachao Mou ◽  
Weilun Zuo ◽  
...  
Keyword(s):  
Animal Model ◽  
Bordetella Pertussis ◽  
Whooping Cough ◽  
Rhesus Macaques ◽  
Public Health Problem ◽  
Primate Model ◽  
Pertussis Infection ◽  
Cellular Immune ◽  
Infant Rhesus ◽  
Human Primate

Abstract Background: The prevalent resurgence of pertussis recently creates a vital public health problem worldwide. To understand the pertussis pathogenesis and host response to both pathogen and vaccine, a suitable pertussis animal model, particularly a non-human primate model, is necessary. Recently, a successful non-human primate pertussis model of baboons have been established. However, though the rhesus macaques have been proven to be ideal animal models for several infectious diseases, the infectious model of pertussis has not been established on it. The previous studies on rhesus macaque models of pertussis were performed in 1920s-1930s with limited experimental details. Recent monkey pertussis models failed to be established because the typical clinic syndrome and transmission were not investigated.Methods: In the present study, infant rhesus macaques were challenged with Bordetella pertussis (B.p) using the aerosol method to evaluate the feasibility of using it as an animal model of pertussis infection.Results: Upon aerosol infection, monkeys infected with the recent clinically isolated B.p strain 2016-CY-41 developed typical whooping cough, leukocytosis, bacteria-positive nasopharyngeal wash (NPW), and inter-animal transmission. Both humoral and cellular immune responses were induced by B.pertussis.Conclusion: These results demonstrate that a model of pertussis infection was successfully established in infant rhesus macaques, which provides a valuable platform to study pertussis pathogenesis and evaluate vaccine candidates.

scholarly journals Infant rhesus macaques as a non-human primate model of Bordetella pertussis infection

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Wenwen Jiang ◽  
Chen Wei ◽  
Dachao Mou ◽  
Weilun Zuo ◽  
Jiangli Liang ◽  
...  
Keyword(s):  
Animal Model ◽  
Bordetella Pertussis ◽  
Clinical Symptoms ◽  
Whooping Cough ◽  
Rhesus Macaques ◽  
Public Health Problem ◽  
Primate Model ◽  
Pertussis Infection ◽  
Infant Rhesus ◽  
Human Primate

Abstract Background The prevalent resurgence of pertussis has recently become a critical public health problem worldwide. To understand pertussis pathogenesis and the host response to both the pathogen and vaccines, a suitable pertussis animal model, particularly a non-human primate model, is necessary. Recently, a non-human primate pertussis model was successfully established with baboons. Rhesus macaques have been shown to be ideal animal models for several infectious diseases, but a model of infectious pertussis has not been established in these organisms. Studies on rhesus macaque models of pertussis were performed in the 1920s–1930s, but limited experimental details are available. Recent monkey pertussis models have not been successful because the typical clinical symptoms and transmission have not been achieved. Methods In the present study, infant rhesus macaques were challenged with Bordetella pertussis (B.p) using an aerosol method to evaluate the feasibility of this system as an animal model of pertussis. Results Upon aerosol infection, monkeys infected with the recently clinically isolated B.p strain 2016-CY-41 developed the typical whooping cough, leukocytosis, bacteria-positive nasopharyngeal wash (NPW), and interanimal transmission of pertussis. Both systemic and mucosal humoral responses were induced by B.p. Conclusion These results demonstrate that a model of pertussis was successfully established in infant rhesus macaques. This model provides a valuable platform for research on pertussis pathogenesis and evaluation of vaccine candidates.

Spondyloarthritis in Taihangshan Macaques (Macaca mulatta tcheliensis)

2021 ◽  
pp. 1-8
Author(s):  
Ke-Li Pang ◽  
Qin-Qin Jin ◽  
Zan-An Yuan ◽  
Zhen-Jing Kuang ◽  
Ji-Qi Lu ◽  
...  
Keyword(s):  
Ankylosing Spondylitis ◽  
Rhesus Macaque ◽  
Macaca Mulatta ◽  
Nature Reserve ◽  
Rhesus Macaques ◽  
Pubic Symphysis ◽  
Primate Species ◽  
Human Beings ◽  
Captive Populations ◽  
Nonhuman Primate Species

The rhesus macaque (<i>Macaca mulatta</i>) is the most widely distributed nonhuman primate species, and captive populations play an important role in biomedical research due to close phylogenetic and physiological similarity to human beings. However, to our best knowledge, the spondyloarthritis (SpA) in rhesus macaques has been exclusively reported in captive or semicaptive populations rather than wild counterparts. In the present study, we report 2 cases of SpA observed in Taihangshan macaques (<i>Macaca mulatta tcheliensis</i>) inhabiting the Taihangshan Macaque National Nature Reserve, Henan Province, China. Among these 2 cases, one can be diagnosed as ankylosing spondylitis (AS) following accepted medical criteria, and another case showed evident fusion at the pubic symphysis which could be specific to rhesus macaque AS. We discuss the potential causes leading directly or indirectly to the development of SpA.

CLINICAL AND LABORATORY PECULIARITIES OF WHOOPING COUGH IN CHILDREN OF DIFFERENT AGE GROUPS

2020 ◽  
Vol 99 (6) ◽  
pp. 98-104
Author(s):  
I.V. Babachenko ◽  
◽  
Y.V. Nesterova ◽  
N.V. Skripchenko ◽  
◽  
...  
Keyword(s):  
Whooping Cough ◽  
Severe Disease ◽  
Respiratory Rhythm ◽  
Age Groups ◽  
Low Frequency ◽  
Pertussis Infection ◽  
Group 2 ◽  
Sick Children ◽  
Hematological Changes ◽  
Group 1

Objective of the research: to present the clinical and laboratory peculiarities of modern whooping cough in hospitalized children of different ages. Materials and methods: сlinical and laboratory characteristics of whooping cough were analyzed in 88 hospitalized sick children aged 1 month to 18 years in groups of children: group 1 – children under 1 year old; group 2 – children 1–6 years old; group 3 – children 7–17 years old. DNA of causative agents of pertussis infection was isolated by PCR in nasopharyngeal swabs using a commercial kit AmpliSens®Bordetella multi-FL (Moscow). Results: children of group 1 in 90% (n=43) of cases were not vaccinated against whooping cough, severe forms were recorded in 17% (n=8) of children of the 1st year of life, and in 15% (n=7) – due to respiratory rhythm disturbances. The diagnosis was confirmed by PCR in 94% (n=45) of children, leukocytosis with lymphocytosis was detected in 81,5% (n=101). Along with hematological changes typical for whooping cough, 79% (n=38) of patients in the first year of life had thrombocytosis (>400×109/l), which was most pronounced in severe disease course 511,5 [425; 568,5]×109/l vs 421 [347; 505,5]×109/l; p<0,05, which has no tendency to decrease throughout the entire observation period and correlates with the level of leukocytes (rs=0,69; p<0,001). Patients over 7 years old in 88% (n=21) of cases were vaccinated against whooping cough, but 79% (n=27) hemograms had no characteristic changes, which, along with a low frequency of confirmation of the diagnosis by PCR 22% (n=4), made it difficult to diagnose whooping cough. Conclusion: children over 7 years of age may not have characteristic hematological changes and PCR diagnostics are insufficiently effective, which contributes to the spread of whooping cough in family foci.

scholarly journals Cross-Sectional Study of Varicella Zoster Virus Immunity in Healthy Korean Children Assessed by Glycoprotein Enzyme-Linked Immunosorbent Assay and Fluorescent Antibody to Membrane Antigen Test

Vaccines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 492
Author(s):  
Yunhwa Kim ◽  
Ji-Young Hwang ◽  
Kyung-Min Lee ◽  
Eunsil Lee ◽  
Hosun Park
Keyword(s):  
South Korea ◽  
Immunosorbent Assay ◽  
Fluorescent Antibody ◽  
Seroconversion Rate ◽  
Geometric Mean ◽  
Varicella Vaccine ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Children ◽  
Korean Children ◽  
Post Vaccination

The prevalence of varicella is especially high among children in the age group of 4–6 years in South Korea, regardless of vaccination. We investigated the immune status of healthy children enrolled in day-care centers and compared pre- and post-vaccination immunity. Antibody titers were measured using a glycoprotein enzyme-linked immunosorbent assay (gpEIA) kit, and the seroconversion rate was assessed using a fluorescent antibody to membrane antigen (FAMA) test. Among 541 vaccinated children, 109 (20.1%) had breakthrough varicella. However, 13 (72.2%) of the 18 unvaccinated children had a history of varicella. The gpEIA geometric mean titers (GMTs) of pre- and 5 weeks post-vaccination in 1-year-old children were 14.7 and 72 mIU/mL, respectively, and the FAMA seroconversion rate was 91.1%. The gpEIA GMTs of 2-, 3-, 4-, 5-, and 6-year-old children were 104.1, 133.8, 223.5, 364.1, and 353.0 mIU/mL, respectively. Even though the gpEIA GMT increased with age, the pattern of gpEIA titer distribution in 4- to 6-year-old vaccinees without varicella history represented both waning immunity and natural boosting immunity. These results suggest that some vaccinees are vulnerable to varicella infection. Therefore, it is necessary to consider a two-dose varicella vaccine regimen in South Korea.

scholarly journals Extensive Countrywide Field Investigation of Somatic Cell Counts and Total Bacterial Counts in Bulk-Tank Raw Milk in Sheep Flocks in Greece

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 268
Author(s):  
Daphne T. Lianou ◽  
Charalambia K. Michael ◽  
Natalia G. C. Vasileiou ◽  
Efthymia Petinaki ◽  
Peter J. Cripps ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Geometric Mean ◽  
Cell Counting ◽  
Lactation Period ◽  
Mechanical Ventilators ◽  
Bulk Tank Milk ◽  
Bacterial Counts ◽  
Somatic Cell Counts ◽  
Cell Counts ◽  
Bulk Tank

Objectives were to investigate somatic cell counts (SCC) and total bacterial counts (TBC) in the raw bulk-tank milk of sheep flocks in Greece, to study factors potentially influencing increased SCC and TBC in the bulk-tank milk of sheep and to evaluate possible associations of SCC and TBC with milk content. Throughout Greece, 325 dairy sheep flocks were visited for collection of milk sampling for somatic cell counting, microbiological examination and composition measurement. Geometric mean SCC were 0.488 × 106 cells mL−1; geometric mean TBC were 398 × 103 cfu mL−1; 228 staphylococcal isolates were recovered form 206 flocks (63.4%). Multivariable analyses revealed annual incidence risk of clinical mastitis, age of the farmer and month into lactation period (among 53 variables) to be significant for SCC > 1.0 × 106 cells mL−1 and month into lactation period at sampling and availability of mechanical ventilators (among 58 variables) to be significant for TBC > 1500 × 103 cfu mL−1. Negative correlation of SCC with fat, total protein and lactose and positive correlation of SCC with added water were found. With SCC > 1.0 × 106 cells mL−1, significant reduction of protein content (2%) was observed, whilst in flocks with SCC > 1.5 × 106 cells mL−1, significantly lower annual milk production per ewe (42.9%) was recorded.

Sign in / Sign up

Export Citation Format

Share Document