scholarly journals Emergence and rapid global dissemination of CTX-M-15-associated Klebsiella pneumoniae strain ST307

2018 ◽  
Author(s):  
Kelly L. Wyres ◽  
Jane Hawkey ◽  
Marit A.K. Hetland ◽  
Aasmund Fostervold ◽  
Ryan R. Wick ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Carbapenem Resistance ◽  
Beta Lactamase ◽  
First Report ◽  
Extended Spectrum Beta Lactamase ◽  
Esbl Gene ◽  
Resistance Determinants ◽  
Extended Spectrum ◽  
Globally Distributed

AbstractRecent reports indicate the emergence of a new carbapenemase producing Klebsiella pneumoniae clone, ST307. Here we show that ST307 emerged in the mid-1990s (nearly 20 years prior to its first report), is already globally distributed and is intimately associated with a conserved plasmid harbouring the blaCTX-M-15 extended-spectrum beta-lactamase (ESBL) gene plus other antimicrobial resistance determinants. Our findings support the need for enhanced surveillance of this widespread ESBL clone in which carbapenem resistance is now emerging.

scholarly journals Incidence and Antimicrobial Susceptibility of Escherichia coli and Klebsiella pneumoniae with Extended-Spectrum β-Lactamases in Community- and Hospital-Associated Intra-Abdominal Infections in Europe: Results of the 2008 Study for Monitoring Antimicrobial Resistance Trends (SMART)

2010 ◽  
Vol 54 (7) ◽  
pp. 3043-3046 ◽  
Author(s):  
Stephen P. Hawser ◽  
Samuel K. Bouchillon ◽  
Daryl J. Hoban ◽  
Robert E. Badal ◽  
Rafael Cantón ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Susceptibility ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Abdominal Infections

ABSTRACT From 2002 to 2008, there was a significant increase in extended-spectrum beta-lactamase (ESBL)-positive Escherichia coli isolates in European intra-abdominal infections, from 4.3% in 2002 to 11.8% in 2008 (P < 0.001), but not for ESBL-positive Klebsiella pneumoniae isolates (16.4% to 17.9% [P > 0.05]). Hospital-associated isolates were more common than community-associated isolates, at 14.0% versus 6.5%, respectively, for E. coli (P < 0.001) and 20.9% versus 5.3%, respectively, for K. pneumoniae (P < 0.01). Carbapenems were consistently the most active drugs tested.

scholarly journals PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF FIVE ANTIMICROBIAL RESISTANCE GENES ASSOCIATED WITH KLEBSIELLA PNEUMONIAE ISOLATED FROM BURN INFECTION PATIENTS

2021 ◽  
Vol 9 (3) ◽  
pp. 378-387
Author(s):  
Mohsin Razzaq Azeez ◽  
◽  
Ahmed Abduljabbar Jaloob Aljanaby ◽  
Ilkay Corak Ocal ◽  
◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Resistance Genes ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Genotypic Characterization ◽  
Antimicrobial Resistance Genes ◽  
Phenotypic And Genotypic Characterization

The current study was carried out for the phenotypic and genotypic characterization of five antimicrobial resistance-associated genes in Klebsiella pneumoniae isolated from burn infection patients. Total one hundred three (103) bacterial samples (strains) were isolated from the 103 burn infection patients admitted at Middle Euphrates Burns Center of AL-Kufa City Iraq. Out of total isolated bacterial samples (103), there were 31 isolates (30%) identified as Pseudomonas sp., 23 isolates (22.3%) as K. pneumonia, 22 isolates as Staphylococcus sps. (21.4%), 11 isolates as E. coli (10.6%), 8 isolates as Acinetobacter sps. (7.8%), 5 isolates as Enterobacter sps. (4.9%), while the lowest prevalence (3 isolates) was reported for the Proteus spp. (3%). The antimicrobial sensitivity test indicated that all isolated K. pneumoniae have resistant (100%) against standard antibiotic Amoxicillin. While Imipenem is the only antibiotic that can inhibit the growth of all 23 isolates. Further, according to the phenotypic detection method, there were 14 isolates (61%) capable of production of extended spectrum beta lactamase (ESBL). Genotypic method to detect the presence of five antibiotic resistance genes by polymerase chain reaction proved that 13 isolates (56.5%) were Tem gene, 18 isolates (78.2%) were positive for Shv gene, 8 isolates (34.7%) were positive Ctxm gene, three isolates (13%) were positive for Oxa gene and 10 isolates (43.7%) positive for AmpC gene. Results of the study can be concluded that K. pneumoniae is the second causative agent that causes burn infection and has higher antibiotics resistance. Extended spectrum beta lactamase of K. pneumoniae was higher prevalence in burn infection and harbored many beta lactamase genes.

scholarly journals Population Genomic Analysis of 1,777 Extended-Spectrum Beta-Lactamase-Producing Klebsiella pneumoniae Isolates, Houston, Texas: Unexpected Abundance of Clonal Group 307

mBio ◽  
2017 ◽  
Vol 8 (3) ◽  
Author(s):  
S. Wesley Long ◽  
Randall J. Olsen ◽  
Todd N. Eagar ◽  
Stephen B. Beres ◽  
Picheng Zhao ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Population Based ◽  
Beta Lactamase ◽  
Clonal Group ◽  
Extended Spectrum Beta Lactamase ◽  
Population Genomic ◽  
Extended Spectrum ◽  
Experimental Mouse ◽  
Human Infections

ABSTRACT Klebsiella pneumoniae is a major human pathogen responsible for high morbidity and mortality rates. The emergence and spread of strains resistant to multiple antimicrobial agents and documented large nosocomial outbreaks are especially concerning. To develop new therapeutic strategies for K. pneumoniae , it is imperative to understand the population genomic structure of strains causing human infections. To address this knowledge gap, we sequenced the genomes of 1,777 extended-spectrum beta-lactamase-producing K. pneumoniae strains cultured from patients in the 2,000-bed Houston Methodist Hospital system between September 2011 and May 2015, representing a comprehensive, population-based strain sample. Strains of largely uncharacterized clonal group 307 (CG307) caused more infections than those of well-studied epidemic CG258. Strains varied markedly in gene content and had an extensive array of small and very large plasmids, often containing antimicrobial resistance genes. Some patients with multiple strains cultured over time were infected with genetically distinct clones. We identified 15 strains expressing the New Delhi metallo-beta-lactamase 1 (NDM-1) enzyme that confers broad resistance to nearly all beta-lactam antibiotics. Transcriptome sequencing analysis of 10 phylogenetically diverse strains showed that the global transcriptome of each strain was unique and highly variable. Experimental mouse infection provided new information about immunological parameters of host-pathogen interaction. We exploited the large data set to develop whole-genome sequence-based classifiers that accurately predict clinical antimicrobial resistance for 12 of the 16 antibiotics tested. We conclude that analysis of large, comprehensive, population-based strain samples can assist understanding of the molecular diversity of these organisms and contribute to enhanced translational research. IMPORTANCE Klebsiella pneumoniae causes human infections that are increasingly difficult to treat because many strains are resistant to multiple antibiotics. Clonal group 258 (CG258) organisms have caused outbreaks in health care settings worldwide. Using a comprehensive population-based sample of extended-spectrum beta-lactamase (ESBL)-producing K. pneumoniae strains, we show that a relatively uncommon clonal type, CG307, caused the plurality of ESBL-producing K. pneumoniae infections in our patients. We discovered that CG307 strains have been abundant in Houston for many years. As assessed by experimental mouse infection, CG307 strains were as virulent as pandemic CG258 strains. Our results may portend the emergence of an especially successful clonal group of antibiotic-resistant K. pneumoniae .

scholarly journals Phenotypic and genotypic characterization of carbapenem resistance mechanisms in Klebsiella pneumoniae from blood culture specimens: A study from North India

2018 ◽  
Vol 10 (02) ◽  
pp. 125-129 ◽  
Author(s):  
Varsha Gupta ◽  
Ritu Garg ◽  
Karthikeyan Kumaraswamy ◽  
Priya Datta ◽  
Gursimran Kaur Mohi ◽  
...  
Keyword(s):  
Blood Culture ◽  
Klebsiella Pneumoniae ◽  
Carbapenem Resistance ◽  
North India ◽  
Beta Lactamase ◽  
Mechanisms Of Resistance ◽  
Beta Lactamases ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Geographical Regions

ABSTRACT BACKGROUND: Emergence of carbapenem resistance among Enterobacteriaceae in different geographical regions is of great concern as these bacteria are easily transmissible among patients. Carbapenem-resistance in Enterobacteriaceae is due to production of carbapenemases of various classes and hyper production of the ESBLs (Extended spectrum beta lactamases) and Amp C beta lactamases with reduced cell wall permeability mechanisms. Phenotypic detection and differentiation is important for proper infection control and appropriate patient management. This study was done to know the prescence of various beta lactamases and carbapenemases with other mechanisms of resistance in Klebsiella pneumoniae isolates. MATERIALS AND METHODS: 50 non-duplicate carbapenem resistant isolates of Klebsiella pneumoniae from blood culture specimens were included and various mechanisms of resistance were studied based on phenotypic and genotypic methods. RESULTS: Out of 50 isolates, 39 (78%) of K.pneumoniae isolates were Extended Spectrum Beta Lactamase (ESBL) producers based on CLSI guidelines. All 50 showed positive Modified Hodge Test (MHT ) and 32 showed Metallo Beta Lactamase (MBL) by Combined Disc Test (CDT). Four isolates showed AmpC production with porin loss . None of the isolates showed Class A KPC production by CDT . In our study all the 10 isolates evaluated by genotypic technique produced CTX-M group 1 enzyme by multiplex PCR. Seven out of 10 strains which showed positive MBL results were positive for NDM . CONCLUSIONS: Carbapenems are often considered last resort antibiotics in the treatment of infections due to multidrug-resistant organisms. It is therefore mandatory to maintain the clinical efficacy of carbapenems by early detection of various enzymes . For routine clinical laboratories both phenotypic and genotypic tests need to be followed to detect various mechanisms of carbapenem resistance and this is of epidemiological relevance also.

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