scholarly journals Phylogenetic relationships in the genus Avena based on the nuclear pgk1 gene

2018 ◽  
Author(s):  
Yuanying Peng ◽  
Pingping Zhou ◽  
Jun Zhao ◽  
Junzhuo Li ◽  
Shikui Lai ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
Diploid Species ◽  
Phosphoglycerate Kinase ◽  
Kinase Gene ◽  
D Genome ◽  
C Genome ◽  
Structural Divergence ◽  
Close Relationship ◽  
Phosphoglycerate Kinase Gene

AbstractThe phylogenetic relationships among 76 Avena taxa, representing 14 diploids, eight tetraploids, and four hexaploids were investigated by using the nuclear plastid 3-phosphoglycerate kinase gene(pgk1). A significant deletion (131 bp) was detected in all the C genome homoeologues which reconfirmed a major structural divergence between the A and C genomes. Phylogenetic analysis indicated the Cp genome is more closely related to the polyploid species than is the Cv genome. Two haplotypes of pgk1 gene were obtained from most of the AB genome tetraploids. Both types of the barbata group showed a close relationship with the As genome diploid species, supporting the hypothesis that both the A and B genomes are derived from an As genome. Two haplotypes were also detected in A. agadiriana, which showed close relationships with the As genome diploid and the Ac genome diploid, respectively, emphasizing the important role of the Ac genome in the evolution of A. agadiriana. Three homoeologues of thepgK1 gene were detected in five hexaploid accessions. The homoeologues that might represent the D genome were tightly clustered with the tetraploids A. marrocana and A. murphyi, but did not show a close relationship with any extant diploid species.

Demethylation of specific sites in the 5' region of the inactive X-linked human phosphoglycerate kinase gene correlates with the appearance of nuclease sensitivity and gene expression

1988 ◽  
Vol 8 (11) ◽  
pp. 4692-4699
Author(s):  
R S Hansen ◽  
N A Ellis ◽  
S M Gartler
Keyword(s):  
Cell Lines ◽  
X Chromosome ◽  
Transcriptional Activation ◽  
Hybrid Cell ◽  
Methylation Status ◽  
Phosphoglycerate Kinase ◽  
Kinase Gene ◽  
Inactive X Chromosome ◽  
Phosphoglycerate Kinase Gene ◽  
Hypoxanthine Guanine Phosphoribosyltransferase

X8/6T2, a hamster-human hybrid cell line which contains an inactive human X chromosome, was treated with 5-azacytidine and selected for derepression of hypoxanthine-guanine phosphoribosyltransferase. Clones were examined for coreactivation of the phosphoglycerate kinase gene (Pgk). Of 68 of these hybrids, approximately 20% expressed measurable human phosphoglycerate kinase (PGK) activity. A 600-base-pair region of the Pgk 5' CpG cluster was examined for the methylation status of eight CCGG sites (site 1 being 5'-most) in a number of PGK-negative and PGK-positive cell lines. The inactive X chromosome is normally methylated at all eight sites, and this was also true for the majority of X8/6T2 cells. However, several PGK-negative hybrids were demethylated in the site 3 to site 6 region. PGK activity correlated with demethylation at both sites 6 and 7. The data for PGK-positive and -negative hybrids indicate that demethylation at or near site 7 was necessary for reactivation of Pgk. Chromatin sensitivity to MspI digestion in the nuclei of male lymphoblastoid cells and several PGK-positive and PGK-negative hybrids was examined. PGK-positive cell lines were hypersensitive to digestion, while PGK-negative hybrids were resistant. Cleavage at sites 6 and 7 was observed in all PGK-positive cell lines at each MspI concentration examined. Sites 7 and 8 were less accessible to digestion than site 6. Cleavage in the site 2 to site 5 region was observable at the lowest MspI concentration. In most PGK-positive hybrids, a nonspecific endogenous nuclease detected the presence of a hypersensitive region spanning at least 450 base pairs, bounded at the 3' end near HpaII site 6. Nuclease hypersensitivity appears to be related to promoter activity, because sites 7 and 8 are in transcribed regions of the gene. These data indicate that specific sites within the CpG cluster have a dominant controlling influence over the Pgk promoter conformation and the transcriptional activation of Pgk.

scholarly journals Nucleotide Sequence of a Wheat Chloroplastic Phosphoglycerate Kinase Gene

1995 ◽  
Vol 107 (4) ◽  
pp. 1483-1484 ◽  
Author(s):  
P. G. Jones ◽  
C. A. Raines ◽  
J. C. Lloyd
Keyword(s):  
Nucleotide Sequence ◽  
Phosphoglycerate Kinase ◽  
Kinase Gene ◽  
Phosphoglycerate Kinase Gene

Stimulation of Yeast 3-Phosphoglycerate Kinase Gene Promoter by Paraquat

2000 ◽  
Vol 270 (3) ◽  
pp. 1036-1040 ◽  
Author(s):  
Neville Vassallo ◽  
Dolores R. Galea ◽  
William H. Bannister ◽  
Rena Balzan
Keyword(s):  
Phosphoglycerate Kinase ◽  
Gene Promoter ◽  
Kinase Gene ◽  
Phosphoglycerate Kinase Gene ◽  
Stimulation Of

The testis-specific phosphoglycerate kinase gene pgk-2 is a recruited retroposon

1987 ◽  
Vol 7 (9) ◽  
pp. 3107-3112
Author(s):  
P H Boer ◽  
C N Adra ◽  
Y F Lau ◽  
M W McBurney
Keyword(s):  
Nucleotide Sequence ◽  
Gene Duplication ◽  
X Chromosome ◽  
Phosphoglycerate Kinase ◽  
Sperm Cells ◽  
Kinase Gene ◽  
Evolutionary Diversification ◽  
Key Enzyme ◽  
Phosphoglycerate Kinase Gene ◽  
Kinase A

In both humans and mice, two genes encode phosphoglycerate kinase, a key enzyme in the glycolytic pathway. The pgk-1 gene is expressed in all somatic cells, is located on the X chromosome, and contains 10 introns. The pgk-2 gene is expressed only in sperm cells, is located on an autosome, and has no introns. The nucleotide sequence of the pgk-2 gene suggests that it arose from pgk-1 more than 100 million years ago by RNA-mediated gene duplication. The pgk-2 gene may, then, be a transcribed retroposon. Thus, gene duplication by retroposition may have been used as a mechanism for evolutionary diversification.

Immune responses in rats and sheep induced by a DNA vaccine containing the phosphoglycerate kinase gene of Fasciola hepatica and liver fluke infection

2016 ◽  
Vol 61 (2) ◽  
Author(s):  
Agnieszka Wesołowska ◽  
Anna Zawistowska-Deniziak ◽  
Luke J. Norbury ◽  
Przemysław Wilkowski ◽  
Kamil Januszkiewicz ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Phosphoglycerate Kinase ◽  
Kinase Gene ◽  
Phosphoglycerate Kinase Gene ◽  
Fluke Infection ◽  
Liver Fluke Infection

AbstractImmune responses of rats and sheep following vaccination with cDNA encoding phosphoglycerate kinase of

Sign in / Sign up

Export Citation Format

Share Document