scholarly journals Exploration of miRNA-mediated fertility regulation network of cytoplasmic male sterility during flower bud development in soybean

2018 ◽  
Author(s):  
Ding Xianlong ◽  
Zhang Hao ◽  
Ruan Hui ◽  
Li Yanwei ◽  
Chen Linfeng ◽  
...  
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Small Rna ◽  
High Throughput Sequencing ◽  
Expression Patterns ◽  
Integrated Analysis ◽  
Flower Bud ◽  
Bud Development ◽  
Cms Line ◽  
Flower Bud Development

ABSTRACTCytoplasmic male sterility (CMS) plays an important role in the production of soybean hybrid seeds. MicroRNAs (miRNAs) are a class of non-coding endogenous ~21 nt small RNAs that play crucial roles in flower and pollen development by targeting genes in plants. Here, two small RNA libraries and two degradome libraries were constructed from the flower buds of the soybean CMS line NJCMS1A and its restorer (Rf) line NJCMS1C. Following high-throughput sequencing, 558 known miRNAs, 103 novel miRNAs on the other arm of known pre-miRNAs, 10 novel miRNAs, and a number of base-edited miRNAs were identified. Among the identified miRNAs, 76 differentially expressed miRNAs were discovered with greater than two-fold changes between NJCMS1A and NJCMS1C. By degradome analysis, a total of 466 distinct transcripts targeted by 200 miRNAs and 122 distinct transcripts targeted by 307 base-edited miRNAs were detected. Further integrated analysis of transcriptome and small RNA found some miRNAs and their targets’ expression patterns showing a negative correlation, such as miR156b-GmSPL and miR4413b-GmPPR. Previous reports showed that these targets might be related to flower bud development, suggesting that miRNAs might act as regulators of soybean CMS fertility. These findings may provide a better understanding of the miRNA-mediated regulatory networks in CMS mechanisms of soybean.

scholarly journals Cytoplasmic Male Sterility-Associated Mitochondrial Gene orf312 Derived from Rice (Oryza sativa L.) Cultivar Tadukan

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ayumu Takatsuka ◽  
Tomohiko Kazama ◽  
Kinya Toriyama
Keyword(s):  
Oryza Sativa ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Oryza Sativa L ◽  
Mitochondrial Gene ◽  
Expression Patterns ◽  
Promising Candidate ◽  
Illumina Hiseq ◽  
Reading Frame ◽  
Cms Line

Abstract Background Cytoplasmic male sterility (CMS) is a trait associated with non-functional pollen or anthers, caused by the interaction between mitochondrial and nuclear genes. Findings A Tadukan-type CMS line (TAA) and a restorer line (TAR) were obtained by successive backcrossing between the Oryza sativa cultivars Tadukan (a cytoplasmic donor) and Taichung 65 (a recurrent pollen parent). Using Illumina HiSeq, we determined whole-genome sequences of the mitochondria of TAA and screened the mitochondrial genome for the presence of open reading frame (orf) genes specific to this genome. One of these orf genes, orf312, showed differential expression patterns in TAA and TAR anthers at the meiotic and mature stages, with transcript amounts in TAR being less than those in TAA. The orf312 gene is similar to the previously described orf288, a part of which is among the components comprising WA352, a chimeric CMS-associated gene of wild-abortive-type CMS. Conclusions The orf312 gene is a promising candidate for CMS-associated gene in TAA.

scholarly journals Metabolomics Studies on Cytoplasmic Male Sterility during Flower Bud Development in Soybean

2019 ◽  
Vol 20 (12) ◽  
pp. 2869 ◽  
Author(s):  
Xianlong Ding ◽  
Xuan Wang ◽  
Qiang Li ◽  
Lifeng Yu ◽  
Qijian Song ◽  
...  
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activities ◽  
Ros Scavenging ◽  
Pollen Abortion ◽  
Flower Buds ◽  
Flower Bud ◽  
Cms Line ◽  
Metabolite Content

Abnormal reactive oxygen species (ROS) may mediate cytoplasmic male sterility (CMS). To observe the effect of ROS on soybean CMS, metabolite content and antioxidant enzyme activity in the flower buds between soybean N8855-derived CMS line and its maintainer were compared. Of the 612 metabolites identified, a total of 74 metabolites were significantly differentiated in flower buds between CMS line and its maintainer. The differential metabolites involved 32 differential flavonoids, 13 differential phenolamides, and 1 differential oxidized glutathione (GSSG) belonging to a non-enzymatic ROS scavenging system. We observed lower levels of flavonoids and antioxidant enzyme activities in flower buds of the CMS line than in its maintainer. Our results suggest that deficiencies of enzymatic and non-enzymatic ROS scavenging systems in soybean CMS line cannot eliminate ROS in anthers effectively, excessive accumulation of ROS triggered programmed cell death and ultimately resulted in pollen abortion of soybean CMS line.

scholarly journals Discovery of Four Novel ORFs Responsible for Cytoplasmic Male Sterility (CMS) in Cotton (Gossypium hirsutum L.) through Comparative Analysis of the Mitochondrial Genomes of Four Isoplasmic Lines

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 765
Author(s):  
Min Li ◽  
Li Chen ◽  
Danfeng Tang ◽  
Xiaofang Liao ◽  
Xiangjun Kong ◽  
...  
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Ribosomal Proteins ◽  
High Throughput Sequencing ◽  
Open Reading Frames ◽  
Maintainer Line ◽  
Protein Coding ◽  
Synonymous Mutations ◽  
Protein Coding Genes ◽  
Cms Line

Cytoplasmic male sterility (CMS) is an important feature for achieving heterosis in the development of hybrid crops. Mitochondria contribute to CMS, especially via mitochondrial DNA (mtDNA) rearrangements and chimeric genes. However, the mechanisms of CMS have not been fully elucidated, and the isonuclear alloplasmic lines used in previous studies have limited utility in cotton CMS research. In this study, three CMS lines (J4A-1, J4A-2 and J4A-3) and their isoplasmic maintainer line (J4B) were analyzed for mtDNA structural differences via high-throughput sequencing. The results showed that mtDNA was conserved (with similarities higher than 99%) among the three CMS lines and their isoplasmic maintainer line. All lines harbored 36 known protein-coding genes, 3 rRNAs, and 15 tRNAs. The protein-coding genes with non-synonymous mutations mainly encoded two types of proteins: ATPase and ribosomal proteins. Four new open reading frames (ORFs) (orf116b, orf186a-1, orf186a-2 and orf305a) were identified as candidate ORFs responsible for CMS. Two of the ORFs (orf186a-1 and orf186a-2) were identified as orf4 and orf4-2 of the upland cotton CMS line 2074A (a line with Gossypium harknessii Brandegee CMS-D2-2 cytoplasm), respectively. These findings provide a reference for CMS research in cotton or other crops.

scholarly journals The tuberization signal StSP6A represses flower bud development in potato

2018 ◽  
Vol 70 (3) ◽  
pp. 937-948 ◽  
Author(s):  
Faline D M Plantenga ◽  
Sara Bergonzi ◽  
José A Abelenda ◽  
Christian W B Bachem ◽  
Richard G F Visser ◽  
...  
Keyword(s):  
Flower Bud ◽  
Bud Development ◽  
Flower Bud Development

scholarly journals Storage Temperature and Duration Affect Flower Bud Development, Shoot Emergence, and Flowering of Leucocoryne coquimbensis F. Phil.

1998 ◽  
Vol 123 (4) ◽  
pp. 586-591 ◽  
Author(s):  
Kiyoshi Ohkawa ◽  
Hyeon-Hye Kim ◽  
Emiko Nitta ◽  
Yukinori Fukazawa
Keyword(s):  
No Reduction ◽  
Storage Temperature ◽  
Cut Flower ◽  
Flower Bud ◽  
Bud Development ◽  
Dry Storage ◽  
Flower Stem ◽  
Shoot Emergence ◽  
Flower Bud Development

Leucocoryne, a native to Chile, has violet, blue, or white flowers and is increasing in popularity as a cut flower. The effects of storage temperature and duration on flower bud development, shoot emergence, and anthesis were investigated. Bulbs stored at 20 to 30 °C for 22 weeks produced 3.4 flower stems per bulb between March and April. Bulbs stored at 20 °C flowered earliest, followed by those stored at 25 °C. Bulbs stored at 30 °C flowered last. After 16 weeks of storage at 20 °C, a further 2 weeks dry storage at 15 °C before planting resulted in 1 month earlier flowering with no reduction of the number of flowering stems. As dry storage at 20 °C increased to 11 months, the time to emergence and flowering decreased. After dry storage at 20 °C for 12 months, the primary flower stems aborted and secondary stems then developed. Secondary and tertiary flower stems tend to commence flower bud development after the flower bud on the primary flower stem has reached the gynoecium or anther and ovule stage of initiation.

scholarly journals Sequence analysis of the mtDNA region correlated with Shaan 2A cytoplasmic male sterility in rapeseed (Brassica napus L.)

2012 ◽  
Vol 48 (No. 3) ◽  
pp. 139-142 ◽  
Author(s):  
L. Havlíčková ◽  
V. Čurn ◽  
E. Jozová ◽  
V. Kučera ◽  
M. Vyvadilová ◽  
...  
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Molecular Level ◽  
Brassica Napus L ◽  
Cms Line ◽  
Breeding Programmes ◽  
Improved Cultivars ◽  
Hybrid Cultivar ◽  
Fertility Restorers ◽  
Selection Of

Until now in Europe has not been cultivated any hybrid cultivar of oilseed rape based on the Shaan 2A cytoplasmic male sterility (CMS), a widely used CMS system in China. The aim of Czech breeders now is to produce new, improved cultivars of rapeseed based on this CMS system. Sterile Shaan 2A CMS line (S; rf/rf), its corresponding maintainers (N; rf/rf) and fertility restorers (S; Rf/Rf) were analyzed on molecular level for the presence of functional CMS cytoplasm. Two new primer pairs covering CMS-associated gene (so called orf224-1) present in Shaan 2A CMS line were developed and selection capability of the developed primers was successfully evaluated. These primers can be used for early selection of plants with functional Shaan 2A CMS system in breeding programmes.

Sign in / Sign up

Export Citation Format

Share Document