scholarly journals Antimicrobial Activity of Tetrabromobisphenol A (TBBPA) against Staphylococcus aureus Skin Infections

2018 ◽  
Author(s):  
Chang Wang ◽  
Fang Ji ◽  
Fengjie Chen ◽  
Bolei Chen ◽  
Zhen Zhou ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Wound Closure ◽  
Tetrabromobisphenol A ◽  
Gram Positive ◽  
Inflammatory Infiltration ◽  
Gram Positive Bacteria ◽  
Bactericidal Effects ◽  
Selective Antimicrobial Activity

AbstractTetrabromobisphenol A (TBBPA) is a brominated flame retardant with selective antimicrobial activity against Gram-positive bacteria. We show that TBBPA exerts bactericidal effects by damaging the cell wall and membrane of Staphylococcus aureus (SA) without inducing antimicrobial resistance. In vivo skin infection assays indicate that a low dose of TBBPA could contribute to wound closure and attenuate SA infection and inflammatory infiltration. TBBPA has potential for use as an antimicrobial agent against Gram-positive pathogens.

scholarly journals In Vitro and In Vivo Activities of DA-7867, a New Oxazolidinone, against Aerobic Gram-Positive Bacteria

2005 ◽  
Vol 49 (6) ◽  
pp. 2498-2500 ◽  
Author(s):  
Eun Jeong Yoon ◽  
Yeong Woo Jo ◽  
Sung Hak Choi ◽  
Tae Ho Lee ◽  
Jae Keol Rhee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Gram Positive ◽  
Methicillin Resistant ◽  
Gram Positive Bacteria ◽  
Vancomycin Resistant Enterococci ◽  
Infection Models ◽  
Vancomycin Resistant

ABSTRACT In vitro and in vivo activities of DA-7867 were assessed against methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and penicillin-resistant Streptococcus pneumoniae. All isolates were inhibited by DA-7867 at ≤0.78 μg/ml, a four-times-lower concentration than that of inhibition by linezolid. For murine infection models, DA-7867 also exhibited greater efficacy than linezolid against all isolates tested.

scholarly journals Tetrabromobisphenol A (TBBPA) exhibits specific antimicrobial activity against Gram-positive bacteria without detectable resistance

2017 ◽  
Vol 53 (25) ◽  
pp. 3512-3515 ◽  
Author(s):  
Fang Ji ◽  
Chang Wang ◽  
Huimin Wang ◽  
Guangliang Liu ◽  
Bolei Chen ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Tetrabromobisphenol A ◽  
Gram Positive ◽  
Gram Positive Bacteria

We report here the antimicrobial ability of tetrabromobisphenol A (TBBPA) against Gram-positive bacteria without detectable resistance.

scholarly journals RING finger E3 ligase PPP1R11 regulates TLR2 signaling and innate immunity

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Alison C McKelvey ◽  
Travis B Lear ◽  
Sarah R Dunn ◽  
John Evankovich ◽  
James D Londino ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Innate Immunity ◽  
Ring Finger ◽  
E3 Ligase ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Toll Like Receptor 2 ◽  
Ligand Stimulation

Toll-like receptor 2 (TLR2) is a pattern recognition receptor that recognizes many types of PAMPs that originate from gram-positive bacteria. Here we describe a novel mechanism regulating TLR2 protein expression and subsequent cytokine release through the ubiquitination and degradation of the receptor in response to ligand stimulation. We show a new mechanism in which an uncharacterized RING finger E3 ligase, PPP1R11, directly ubiquitinates TLR2 both in vitro and in vivo, which leads to TLR2 degradation and disruption of the signaling cascade. Lentiviral gene transfer or knockdown of PPP1R11 in mouse lungs significantly affects lung inflammation and the clearance of Staphylococcus aureus. There is a negative correlation between PPP1R11 and TLR2 levels in white blood cell samples isolated from patients with Staphylococcus aureus infections. These results suggest that PPP1R11 plays an important role in regulating innate immunity and gram-positive bacterial clearance by functioning, in part, through the ubiquitination and degradation of TLR2.

scholarly journals Antibacterial Properties of Propolis

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2047 ◽  
Author(s):  
Izabela Przybyłek ◽  
Tomasz M. Karpiński
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Properties ◽  
Bacterial Strains ◽  
Complex Composition ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
The World

Researchers are continuing to discover all the properties of propolis due to its complex composition and associated broad spectrum of activities. This review aims to characterize the latest scientific reports in the field of antibacterial activity of this substance. The results of studies on the influence of propolis on more than 600 bacterial strains were analyzed. The greater activity of propolis against Gram-positive bacteria than Gram-negative was confirmed. Moreover, the antimicrobial activity of propolis from different regions of the world was compared. As a result, high activity of propolis from the Middle East was found in relation to both, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) strains. Simultaneously, the lowest activity was demonstrated for propolis samples from Germany, Ireland and Korea.

scholarly journals An in vivo reporter assay for sRNA-directed gene control in Gram-positive bacteria: identifying a novel sRNA target in Staphylococcus aureus

2017 ◽  
Vol 45 (8) ◽  
pp. 4994-5007 ◽  
Author(s):  
Lorraine Ivain ◽  
Valérie Bordeau ◽  
Alex Eyraud ◽  
Marc Hallier ◽  
Stéphane Dreano ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gene Control ◽  
Reporter Assay ◽  
Gram Positive ◽  
Gram Positive Bacteria

scholarly journals Severity of Staphylococcus aureus Infection of the Skin Is Associated with Inducibility of Human β-Defensin 3 but Not Human β-Defensin 2

2010 ◽  
Vol 78 (7) ◽  
pp. 3112-3117 ◽  
Author(s):  
Philipp Zanger ◽  
Johannes Holzer ◽  
Regina Schleucher ◽  
Helmut Scherbaum ◽  
Birgit Schittek ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Clinical Evidence ◽  
Skin Infection ◽  
Mrna Levels ◽  
Surgical Drainage ◽  
Gram Positive ◽  
Positive Skin ◽  
Innate Defense ◽  
Gram Positive Bacteria

ABSTRACT Gram-positive bacteria are the predominant cause of skin infections. Antimicrobial peptides (AMPs) are believed to be of major importance in skin's innate defense against these pathogens. This study aimed at providing clinical evidence for the contribution of AMP inducibility to determining the severity of Gram-positive skin infection. Using real-time PCR, we determined the induction of human β-defensin 2 (HBD-2), HBD-3, and RNase 7 by comparing healthy and lesional mRNA levels in 32 patients with Gram-positive skin infection. We then examined whether AMP induction differed by disease severity, as measured by number of recurrences and need for surgical drainage in patients with Staphylococcus aureus-positive lesions. We found that HBD-2 and -3, but not RNase 7, mRNA expression was highly induced by Gram-positive bacterial infection in otherwise healthy skin. Less induction of HBD-3, but not HBD-2, was associated with more-severe S. aureus skin infection: HBD-3 mRNA levels were 11.4 times lower in patients with more than 6 recurrences (P = 0.01) and 8.8 times lower in patients reporting surgical drainage (P = 0.01) than in the respective baseline groups. This suggests that inducibility of HBD-3 influences the severity of Gram-positive skin infection in vivo. The physiological function of HBD-2 induction in this context remains unclear.

scholarly journals Evaluation of Extracts of Qualea paraensis Ducke for their Antimicrobial, Toxic and Anticholinesterase Activities

2018 ◽  
Vol 10 (4) ◽  
Author(s):  
J. A. Melo1 ◽  
K. M. M. Aroucha1 ◽  
L. P. M. Santos ◽  
C. M. Moraes ◽  
J A. Takahashi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimalarial Activity ◽  
Biological Activities ◽  
Skin Lesions ◽  
Artemia Salina ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Popularly known as red mandioqueira, ‘mandioqueira vermelha’, Qualea paraensis Ducke is a plant species belonging to the family Vochysiaceae, with a natural distribution in the Amazon region. It is used in traditional medicine, by native communities of the Amazon and Bolivia, for the treatment of skin lesions caused by microorganisms. Previous studies of the species have found antimalarial activity in vivo assays. However, studies involving the investigation of numerous biological activities of Q. paraensis are incipient. Biological assays already performed with plants of other species of the genus Qualea have shown promising biological activities. Therefore, this study describes the evaluation of the biological activities (bactericide, fungicide, toxicity, and anticholinesterase) of an ethanolic extract of the bark of Q. paraensis from the state of Roraima, Brazil. For the evaluation of the toxicity of the extract, a system with microcrustacean Artemia salina was used. Antimicrobial activity was tested for the pathogenic groups of fungi (Aspergillus flavus and Fusarium proliferatum), Gram-negative bacteria (Escherichia coli and Salmonella tiphymurium), and Gram-positive bacteria (Staphylococcus aureus and Streptococcus sanguinis). The potential of the extract for the inhibition of the enzyme acetylcholinesterase (AChE) was also evaluated. The assays for determining the antimicrobial activity for Gram-positive bacteria revealed satisfactory IC50 (29.98μg/mL) inhibition values for S. sanguinis strains, showing inhibition of 64.6% of their growth. The assay for S. aureus, however, presented low inhibition. For Gram-negative bacteria, there was moderate inhibition of E. coli strains. The extract showed low toxicity to A. salina and inhibition of 23.66% of the AChE enzyme.

scholarly journals Pharmacodynamics of Telavancin (TD-6424), a Novel Bactericidal Agent, against Gram-Positive Bacteria

2004 ◽  
Vol 48 (8) ◽  
pp. 3043-3050 ◽  
Author(s):  
Sharath S. Hegde ◽  
Noe Reyes ◽  
Tania Wiens ◽  
Nicole Vanasse ◽  
Robert Skinner ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gram Positive ◽  
Time Curve ◽  
Once Daily ◽  
Gram Positive Bacteria ◽  
Resistant Strains ◽  
Vancomycin Resistant ◽  
Dose Dependent

ABSTRACT Telavancin (TD-6424) is a novel lipoglycopeptide that produces rapid and concentration-dependent killing of clinically relevant gram-positive organisms in vitro. The present studies evaluated the in vivo pharmacodynamics of telavancin in the mouse neutropenic thigh (MNT) and mouse subcutaneous infection (MSI) animal models. Pharmacokinetic-pharmacodynamic studies in the MNT model demonstrated that the 24-h area under the concentration-time curve (AUC)/MIC ratio was the best predictor of efficacy. Telavancin produced dose-dependent reduction of thigh titers of several organisms, including methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA), penicillin-susceptible and -resistant strains of Streptococcus pneumoniae, and vancomycin-resistant Enterococcus faecalis. The 50% effective dose (ED50) estimates for telavancin ranged from 0.5 to 6.6 mg/kg of body weight (administered intravenously), and titers were reduced by up to 3 log10 CFU/g from pretreatment values. Against MRSA ATCC 33591, telavancin was 4- and 30-fold more potent (on an ED50 basis) than vancomycin and linezolid, respectively. Against MSSA ATCC 13709, telavancin was 16- and 40-fold more potent than vancomycin and nafcillin, respectively. Telavancin, vancomycin, and linezolid were all efficacious and more potent against MRSA ATCC 33591 in the MSI model compared to the MNT model. This deviation in potency was, however, disproportionately greater for vancomycin and linezolid than for telavancin, suggesting that activity of telavancin is less affected by the immune status. The findings of these studies collectively suggest that once-daily dosing of telavancin may provide an effective approach for the treatment of clinically relevant infections with gram-positive organisms.

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