scholarly journals Transcriptome-wide analysis of auxin-induced carotenoid accumulation in Chlorella microalgae

2018 ◽  
Author(s):  
Faisal Alsenani ◽  
Taylor J. Wass ◽  
Ruijuan Ma ◽  
Eladl Eltanahy ◽  
Michael E. Netzel ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Abscisic Acid ◽  
De Novo ◽  
Higher Plants ◽  
Abiotic Stress Tolerance ◽  
Auxin Signaling ◽  
Rna Seq ◽  
Carotenoid Accumulation ◽  
Close Relationship ◽  
Indole 3 Acetic Acid

AbstractMicroalgae are a commercially viable route for the production of carotenoids, including β-carotene and astaxanthin. In the current study, the commercially relevant microalga, Chlorella sp. BR2 was treated with four plant hormones: indole-3-acetic acid, salicylic acid, abscisic acid and methyl jasmonate, over a range of dosages and screened for enhanced carotenoid production. Indole-3-acetic acid was the only hormone with an inductive effect on carotenoid accumulation. As such, the transcriptome under the condition with the highest carotenoid increase was profiled using RNA-Seq and expressed sequences reconstructed with de novo assembly. This allowed for the profiling of transcriptome-wide changes following auxin treatment, revealing the active pathway components of auxininduced carotenogenesis. Data analysis specified the differentially expressed genes involved in auxin biosynthesis and signal transduction, which suggest a close relationship to equivalent pathways in higher plants. However unlike in plants, the ancient ABP1/SCFSKP2A/IBR5-mediated pathways for auxin response likely acted as the primary signaling route in Chlorella. As carotenoids are precursors for abscisic acid, the findings suggest a causative link between auxin signaling and abiotic stress tolerance.HighlightTranscriptomics of plant hormone-treated Chlorella revealed the active pathway components of auxin-induced carotenogenesis and included the ancient ABP1/SCFSKP2A/IBR5-mediated pathways. The manuscript presents the first documented transcriptomic data of auxin-treated microalgae.

scholarly journals Molecular machinery of auxin synthesis, secretion, and perception in the unicellular chlorophyte alga Chlorella sorokiniana UTEX 1230

2017 ◽  
Author(s):  
Maya Khasin ◽  
Rebecca R. Cahoon ◽  
Kenneth W. Nickerson ◽  
Wayne R. Riekhof
Keyword(s):  
Acetic Acid ◽  
Auxin Transport ◽  
Higher Plants ◽  
Chlorella Sorokiniana ◽  
Auxin Signaling ◽  
Seed Plants ◽  
Auxin Receptor ◽  
Auxin Binding ◽  
Auxin Synthesis ◽  
Indole 3 Acetic Acid

AbstractIndole-3-acetic acid is a ubiquitous small molecule found in all domains of life. It is the predominant and most active auxin in seed plants, where it coordinates a variety of complex growth and development processes. The potential origin of auxin signaling in algae remains a matter of some controversy. In order to clarify the evolutionary context of algal auxin signaling, we undertook a genomic survey to assess whether auxin acts as a signaling molecule in the emerging model chlorophyte Chlorella sorokiniana UTEX 1230. C. sorokiniana produces the auxin indole-3-acetic acid (IAA), which was present in both the cell pellet and in the supernatant at a concentration of ~ 1 nM, and its genome encodes orthologs of genes related to auxin synthesis, transport, and signaling in higher plants. Candidate orthologs for the canonical AUX/IAA signaling pathway were not found; however, auxin-binding protein 1 (ABP1), an alternate auxin receptor, is present and highly conserved at essential auxin binding and zinc coordinating residues. Additionally, candidate orthologs for PIN proteins, responsible for intercellular, vectorial auxin transport in higher plants, were not found, but PILs (PIN-Like) proteins, a recently discovered family that mediates intracellular auxin transport, were identified. The distribution of auxin related gene in this unicellular chlorophyte demonstrates that a core suite of auxin signaling components was present early in the evolution of plants. Understanding the simplified auxin signaling pathways in chlorophytes will aid in understanding phytohormone signaling and crosstalk in seed plants, and in understanding the diversification and integration of developmental signals during the evolution of multicellular plants.

scholarly journals Changes in endogenous levels of IAA and PAA in rice seed during development, prediction of auxin signaling proteins and docking for its binding efficiency with auxin molecules

1970 ◽  
Vol 29 (3) ◽  
pp. 325-337
Author(s):  
V.M. Jhala ◽  
K.S. Chudasama ◽  
K.S. Chudasama ◽  
V.S. Thaker
Keyword(s):  
Acetic Acid ◽  
Higher Plants ◽  
Rice Genome ◽  
Auxin Signaling ◽  
Signaling Proteins ◽  
Rice Seed ◽  
Genome Data ◽  
Sink Size ◽  
Rice Seeds ◽  
Indole 3 Acetic Acid

Indole-3-acetic acid (IAA) and phenyl acetic acid (PAA) are two major physiologically active auxins, found in higher plants. The objective of this study was to evaluate changes in the endogenous levels of IAA and PAA in rice (Oryza sativa) seed and predict probable transporter and efflux (signaling) proteins for docking with auxins from the available rice genome data. IAA and PAA were estimated during rice seed development using sensitive and specific antibodies against each hormone. The levels of IAA and PAA were higher when sink size development was at its peak, suggesting an important role played by the auxin in sink size development of rice seed. Furthermore, auxin binding transporters proteins of rice were confirmed for docking purpose and compared with Arabidopsis and maize proteins. In this context, PAA docked with known IAA proteins; although, docking score of IAA was higher than that of PAA in rice seed. Twelve genes of the auxin efflux carrier (PIN family) of rice were also analysed for phylogeny relatedness with Arabidopsis and maize, to further understanding auxin concentrations and efflux within rice seeds. The predicted PIN proteins for IAA efflux of rice showed docking affinities with PAA, revealed its role in maintaining physiological concentrations of auxins in rice seeds.

scholarly journals Studies on the Growth and Indole-3-Acetic Acid and Abscisic Acid Content of Zea mays Seedlings Grown in Microgravity

1992 ◽  
Vol 100 (2) ◽  
pp. 692-698 ◽  
Author(s):  
Aga Schulze ◽  
Philip J. Jensen ◽  
Mark Desrosiers ◽  
J. George Buta ◽  
Robert S. Bandurski
Keyword(s):  
Zea Mays ◽  
Acetic Acid ◽  
Abscisic Acid ◽  
Acid Content ◽  
Abscisic Acid Content ◽  
Indole 3 Acetic Acid

THE DEVELOPMENT OF ELISA TO DETERMINE ABSCISIC ACID AND INDOLE-3-ACETIC ACID IN BULBOUS CROPS

1986 ◽  
pp. 563-567
Author(s):  
J.M. Franssen ◽  
B.E. Snaar-Jagalska ◽  
C.Th.C. van der Hulst
Keyword(s):  
Acetic Acid ◽  
Abscisic Acid ◽  
Indole 3 Acetic Acid
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