scholarly journals Improved G-AgarTrap: A highly efficient transformation method for intact gemmalings of the liverwortMarchantia polymorpha

2018 ◽  
Author(s):  
Shoko Tsuboyama ◽  
Satoko Nonaka ◽  
Hiroshi Ezura ◽  
Yutaka Kodama
Keyword(s):  
Plant Species ◽  
Transformation Efficiency ◽  
Simple Procedure ◽  
Low Cost ◽  
Transformation Method ◽  
Plant Evolution ◽  
Biological Study ◽  
Marchantia Polymorpha ◽  
Molecular Biological Study ◽  
Key Species

AbstractLiverworts are key species for studies of plant evolution, occupying a basal position among the land plants.Marchantia polymorphahas emerged as a highly studied model liverwort, and many relevant techniques, including genetic transformation, have been established for this species.Agrobacterium-mediated transformation is widely used in many plant species because of its low cost. Recently, we developed a simplifiedAgrobacterium-mediated method for transformingM. polymorpha, known as AgarTrap (agar-utilized transformation with pouring solutions). The AgarTrap procedure, which involves culturing the liverwort tissue in various solutions on a single solid medium, yields up to a hundred independent transformants. AgarTrap is a simple procedure, requiring minimal expertise, cost, and time.Here, we investigated four factors that influence AgarTrap transformation efficiency: (1) humidity, (2) surfactant in the transformation buffer, (3)Agrobacteriumstrain, and (4) light/dark condition. We adapted the AgarTrap protocol for transforming intact gemmalings, achieving an exceptionally high transformation efficiency of 97%. The improved AgarTrap method will enhance the molecular biological study ofM. polymorpha. The present study also provides new possibilities for improving transformation techniques for a variety of plant species.

scholarly journals Phytoremediation Potential of Native Herbaceous Plant Species Growing on a Paradigmatic Brownfield Site

2021 ◽  
Vol 232 (7) ◽  
Author(s):  
N. Matanzas ◽  
E. Afif ◽  
T. E. Díaz ◽  
J. R. Gallego
Keyword(s):  
Plant Species ◽  
Contaminated Soils ◽  
Native Species ◽  
Low Cost ◽  
Plantago Lanceolata ◽  
Herbaceous Plant ◽  
Plant Interactions ◽  
Aerial Parts ◽  
Medicago Lupulina ◽  
Icp Ms

AbstractPhytomanagement techniques using native species allow the recovery of contaminated soils at low cost and circumvent the ecological risks associated with the use of non-native species. In this context, a paradigmatic brownfield megasite highly contaminated by As and Pb was sampled in order to analyze soil–plant interactions and identify plant species with phytoremediation potential. A survey was first carried out in a 20-ha area to obtain an inventory of species growing spontaneously throughout the site. We then performed another survey in the most polluted sub-area (1 ha) within the site. Pseudototal concentrations of contaminants in the soil, aerial parts of the plants, and roots were measured by ICP-MS. A detailed habitat classification was done, and a specific index of coverage was applied by means of a 1-year quadrat study in various sampling stations. Results converged in the selection of six herbaceous species (Dysphania botrys, Lotus corniculatus, Lotus hispidus, Plantago lanceolata, Trifolium repens, Medicago lupulina). All of these plants are fast-growing, thereby making them suitable for use in phytostabilization strategies. Furthermore, they are all easy to grow and propagate and are generally self-sustaining. All six plants showed accumulation factors below 1, thus revealing them as pseudomethallophytes and excluders. However, L. hispidus and M. lupulina showed translocation capacity and are considered worthy of further study.

scholarly journals Peculiar Evolutionary History of miR390-Guided TAS3-Like Genes in Land Plants

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Maria S. Krasnikova ◽  
Denis V. Goryunov ◽  
Alexey V. Troitsky ◽  
Andrey G. Solovyev ◽  
Lydmila V. Ozerova ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Physcomitrella Patens ◽  
Land Plant ◽  
Plant Evolution ◽  
Marchantia Polymorpha ◽  
History Of ◽  
Molecular Machinery ◽  
Specific Mrnas ◽  
Land Plant Evolution ◽  
Phylogenetic Profiling

PCR-based approach was used as a phylogenetic profiling tool to probe genomic DNA samples from representatives of evolutionary distant moss taxa, namely, classes Bryopsida, Tetraphidopsida, Polytrichopsida, Andreaeopsida, and Sphagnopsida. We found relatives of allPhyscomitrella patensmiR390 and TAS3-like loci in these plant taxa excluding Sphagnopsida. Importantly, cloning and sequencing ofMarchantia polymorphagenomic DNA showed miR390 and TAS3-like sequences which were also found among genomic reads ofM. polymorphaat NCBI database. Our data suggest that the ancient plant miR390-dependent TAS molecular machinery firstly evolved to target AP2-like mRNAs in Marchantiophyta and only then both ARF- and AP2-specific mRNAs in mosses. The presented analysis shows that moss TAS3 families may undergone losses of tasiAP2 sites during evolution toward ferns and seed plants. These data confirm that miR390-guided genes coding for ARF- and AP2-specific ta-siRNAs have been gradually changed during land plant evolution.

scholarly journals Toxoplasmosis serology: an efficient hemagglutination procedure to detect IgG and IgM antibodies

1989 ◽  
Vol 31 (4) ◽  
pp. 279-285 ◽  
Author(s):  
M.E. Camargo ◽  
Maria Emilia G. Moura ◽  
P.G. Leser
Keyword(s):  
Simple Procedure ◽  
Low Cost ◽  
Protein A ◽  
Developed Countries ◽  
Serum Samples ◽  
Serum Dilution ◽  
Specific Antibodies ◽  
Igm Antibodies ◽  
Diagnostic Capability ◽  
Acute Toxoplasmosis

In search of an efficient but simple, low cost procedure for the serodiagnosis of Toxoplasmosis, especially suited for routine laboratories facing technical and budget limitations as in less developed countries, the diagnostic capability of Hematoxo® , an hemagglutination test for toxoplasmosis, was evaluated in relation to a battery of tests including IgG- and IgM-immunofluorescence tests, hemagglutination and an IgM-capture enzymatic assay. Detecting a little as 5 I.U. of IgG antitoxoplasma antibodies, Hematoxo® showed a straight agreement as to reactivity and non-reactivity for the 443 non-reactive and the 387 reactive serum samples, included in this study. In 23 cases presenting a serological pattern of acute toxoplasmosis and showing IgM antibodies, Hematoxo® could detect IgM antibodies in 18, indicated by negativation or a significant decrease in titers as a result of treating samples with 2-mercapto-ethanol. However, a neat increase in sensitivity for IgM specific antibodies could be achieved by previously removing IgG from the sample, as demonstrated in a series of acute toxoplasmosis sera. A simple procedure was developed for this purpose, by reconstituting a lyophilized suspension of Protein A - rich Staphylococcus with the lowest serum dilution to be tested. Of low cost and easy to perform, Hematoxo® affords not only a practical qualitative procedure for screening reactors and non-reactors, as in prenatal services, but also quantitative assays that permit to titrate antibodies as well as to identify IgM antibodies.

Using existing site surveillance cameras to automatically measure the installation speed in prefabricated timber construction

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Alireza Ahmadian Fard Fini ◽  
Mojtaba Maghrebi ◽  
Perry John Forsythe ◽  
Travis Steven Waller
Keyword(s):  
Time Window ◽  
Low Cost ◽  
Transformation Method ◽  
Digital Design ◽  
Spatial Transformation ◽  
Exact Matching ◽  
Productivity Measurement ◽  
Content Type ◽  
Surveillance Camera ◽  
Automated Method

PurposeMeasuring onsite productivity has been a substance of debate in the construction industry, mainly due to concerns about accuracy, repeatability and unbiasedness. Such characteristics are central to demonstrate construction speed that can be achieved through adopting new prefabricated systems. Existing productivity measurement methods, however, cannot cost-effectively provide solid and replicable evidence of prefabrication benefits. This research proposes a low-cost automated method for measuring onsite installation productivity of prefabricated systems.Design/methodology/approachFirstly, the captured ultra-wide footages are undistorted by extracting the curvature contours and performing a developed meta-heuristic algorithm to straighten these contours. Then a preprocessing algorithm is developed that could automatically detect and remove the noises caused by vibrations and movements. Because this study aims to accurately measure the productivity the noise free images are double checked in a specific time window to make sure that even a tiny error, which have not been detected in the previous steps, will not been amplified through the process. In the next step, the existing side view provided by the camera is converted to a top view by using a spatial transformation method. Finally, the processed images are compared with the site drawings in order to detect the construction process over time and report the measured productivity.FindingsThe developed algorithms perform nearly real-time productivity computations through exact matching of actual installation process and digital design layout. The accuracy and noninterpretive use of the proposed method is demonstrated in construction of a multistorey cross-laminated timber building.Originality/valueThis study uses footages of an already installed surveillance camera where the camera's features are unknown and then image processing algorithms are deployed to retrieve accurate installation quantities and cycle times. The algorithms are almost generalized and versatile to be adjusted to measure installation productivity of other prefabricated building systems.

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