scholarly journals Anti-inflammatory properties of natural ingredients used in combinations on adjuvant induced arthritis in rats

2018 ◽  
Author(s):  
Amélie St-Pierre ◽  
Véronique Desrosiers ◽  
France Dufresne ◽  
Pierre U. Blier
Keyword(s):  
Rheumatoid Arthritis ◽  
Antioxidant Activity ◽  
Total Antioxidant Activity ◽  
Salix Alba ◽  
Boswellia Serrata ◽  
Freund’S Complete Adjuvant ◽  
Adjuvant Induced Arthritis ◽  
Freund's Complete Adjuvant ◽  
Total Antioxidant ◽  
Anti Inflammatory

AbstractBackground: Rheumatoid arthritis has seen a significant increase in both incidence and prevalence and its treatments show limited efficiency due to their undesirable effects on patient health. Therefore, major interests lie in the development of treatments with drugs derived from plants or other natural sources with little adverse effects as an alternative to current treatments.Hypothesis/Purpose: The present study evaluates the therapeutic effects of glucosamine against rheumatoid arthritis in combination with hyaluronic acid, resin extract of Boswellia serrata or a bark extract of Salix alba on an animal model. We suggest that combinations with plants could improve the attenuation of arthritis symptoms and articular inflammation.Study design: We used Freund’s complete adjuvant on rats as models of rheumatoid arthritis. Individuals were separated into eight experimental groups: a control group without arthritis, one with arthritis and without treatment, and six other groups receiving a daily therapeutic treatment from days 14 to 29.Methods: Hind-paw thickness and arthritis scores were measured at days 0, 3, 6 and 9 post-induction, and then every day from days 12 to 29 with a digital caliper and a score system respectively. At the end of the treatment, the mRNA content of three pro-inflammatory cytokines from cartilage was measured using real-time PCR. The total antioxidant activity was evaluated with an Antioxidant Assay Kit.Results: Treatments with Boswellia serrata and Salix alba (Glu+Hyal A+Bosw, Glu+Bosw+Sal, Glu+Bosw and Glu+Hyal A+Sal) saw significant reductions in hind-paw thickness and arthritis scores at the end of the experiment when compared to the untreated group. Expression of pro-inflammatory gene IL 17A was also reduced, but only the Glu+Hyal A+Sal combination significantly decreased the expression of IL-1β and TNF-α. The total antioxidant activity in blood plasma significantly increased in groups treated with plant extracts.Conclusion: The addition of Boswellia serrata and/or Salix alba attenuates clinical signs of rheumatoid arthritis in Freund’s complete adjuvant-induced arthritis in rats likely due to both their anti-inflammatory and antioxidant properties.

scholarly journals Effect of Disease Modifying Agent on Oxidant and Antioxidant Status in Rheumatoid Arthritis

2018 ◽  
Vol 3 (2) ◽  
pp. 11-15
Author(s):  
Ramprasad Kowsalya ◽  
Ashok Prabhu
Keyword(s):  
Rheumatoid Arthritis ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Antioxidant Status ◽  
Total Antioxidant Activity ◽  
Group I ◽  
Total Antioxidant ◽  
Disease Modifying ◽  
Anti Inflammatory

Background: Rheumatoid Arthritis is a chronic disease and a leading cause of disability in people with advancing age. Disease modifying antirheumatic drugs have become the mainstay of management in rheumatoid arthritis. Among these drugs, methotrexate the most frequently used agent has a proven anti-inflammatory action and is known to slow down the progression of the disease. Hence the present study is undertaken to evaluate the effect of methotrexate on the oxidant and antioxidant status in patients with rheumatoid arthritis on methotrexate.Methods: A total of 40 rheumatoid arthritis patients and 40 controls were enrolled for the study. The rheumatoid arthritis patients were further divided into patients on methotrexate (group I) and patients without methotrexate (group II). The lipid peroxidation index -Malondialdehyde and total antioxidant activity, superoxide dismutase and glutathione levels were analyzed in all the participants.Results: A significant increase in total antioxidant activity (p = 0.048) and decrease in lipid peroxidation (p = 0.04) was found in methotrexate treated arthritis patients compared to patients without methotrexate. A significant negative correlation (p=0.01) was found between MDA and total antioxidant activity in both groups of patients whereas, the antioxidant enzyme superoxide dismutase was positively correlated with lipid peroxide levels. Conclusion: In conclusion low dose methotrexate seems to act as an effective anti-inflammatory drug and may also play an important role in minimizing the oxidative stress in rheumatoid arthritis. Serum total antioxidant activity is significantly raised in subjects with altered liver function profile.

Diurnal changes in the total antioxidant activity of human saliva

2007 ◽  
Vol 33 (3) ◽  
pp. 375-376 ◽  
Author(s):  
M. F. Borisenkov ◽  
L. A. Erunova ◽  
E. M. Lyuseva ◽  
N. V. Pozdeeva
Keyword(s):  
Antioxidant Activity ◽  
Human Saliva ◽  
Diurnal Changes ◽  
Total Antioxidant Activity ◽  
Total Antioxidant
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