scholarly journals High throughput single cell sequencing of both T-cell-receptor-beta alleles

2018 ◽  
Author(s):  
Tomonori Hosoya ◽  
Hongyang Li ◽  
Chia-Jui Ku ◽  
Qingqing Wu ◽  
Yuanfang Guan ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
High Throughput ◽  
Single Cells ◽  
Cell Receptor ◽  
Allelic Exclusion ◽  
Vdj Recombination ◽  
T Cell Receptor Beta ◽  
B And T Lymphocytes ◽  
Receptor Beta

ABSTRACTAllelic exclusion is a vital mechanism for the generation of monospecificity to foreign antigens in B- and T-lymphocytes. Here we developed a high-throughput barcoded method to simultaneously analyze the VDJ recombination status of both mouse T cell receptor beta alleles in hundreds of single cells using Next Generation Sequencing.

scholarly journals Allelic exclusion at DNA rearrangement level is required to prevent coexpression of two distinct T cell receptor beta genes.

1991 ◽  
Vol 174 (4) ◽  
pp. 815-819 ◽  
Author(s):  
J P van Meerwijk ◽  
P Romagnoli ◽  
A Iglesias ◽  
H Bluethmann ◽  
M Steinmetz
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Allelic Exclusion ◽  
Thymic Selection ◽  
T Cell Receptor Beta ◽  
Receptor Beta

In mice double transgenic for functionally rearranged T cell receptor (TCR) V beta 2 and V beta 8.2 genes we found that most T lymphocytes express both TCR beta chains simultaneously. These T cells show no abnormality in thymic selection in vivo and their TCRs are capable of transducing activation signals in vitro. These results indicate that multispecific T cells may appear in the periphery if allelic exclusion of TCR beta genes is not established at the level of gene rearrangement.

scholarly journals The T Cell Receptor (TRB) Locus in Tursiops truncatus: From Sequence to Structure of the Alpha/Beta Heterodimer in the Human/Dolphin Comparison

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 571
Author(s):  
Giovanna Linguiti ◽  
Sofia Kossida ◽  
Ciro Leonardo Pierri ◽  
Joumana Jabado-Michaloud ◽  
Geraldine Folch ◽  
...  
Keyword(s):  
Amino Acids ◽  
T Cell ◽  
T Cell Receptor ◽  
Tursiops Truncatus ◽  
Cell Receptor ◽  
Sequence Alignments ◽  
T Cell Receptor Beta ◽  
Alpha Beta ◽  
Receptor Beta ◽  
Complementarity Determining Region

The bottlenose dolphin (Tursiops truncatus) belongs to the Cetartiodactyla and, similarly to other cetaceans, represents the most successful mammalian colonization of the aquatic environment. Here we report a genomic, evolutionary, and expression study of T. truncatus T cell receptor beta (TRB) genes. Although the organization of the dolphin TRB locus is similar to that of the other artiodactyl species, with three in tandem D-J-C clusters located at its 3′ end, its uniqueness is given by the reduction of the total length due essentially to the absence of duplications and to the deletions that have drastically reduced the number of the germline TRBV genes. We have analyzed the relevant mature transcripts from two subjects. The simultaneous availability of rearranged T cell receptor α (TRA) and TRB cDNA from the peripheral blood of one of the two specimens, and the human/dolphin amino acids multi-sequence alignments, allowed us to calculate the most likely interactions at the protein interface between the alpha/beta heterodimer in complex with major histocompatibility class I (MH1) protein. Interacting amino acids located in the complementarity-determining region according to IMGT numbering (CDR-IMGT) of the dolphin variable V-alpha and beta domains were identified. According to comparative modelization, the atom pair contact sites analysis between the human MH1 grove (G) domains and the T cell receptor (TR) V domains confirms conservation of the structure of the dolphin TR/pMH.

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