scholarly journals ‘In plaque-mass spectrometry imaging’ reveals a major metabolic shift towards odd-chain fatty acid lipids induced by host-virus interactions

2018 ◽  
Author(s):  
Guy Schleyer ◽  
Nir Shahaf ◽  
Carmit Ziv ◽  
Yonghui Dong ◽  
Roy A. Meoded ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid ◽  
Mass Spectrometry Imaging ◽  
Infection Process ◽  
Chain Fatty Acid ◽  
Model Systems ◽  
Metabolic Shift ◽  
Spatiotemporal Resolution ◽  
Host Virus Interactions ◽  
Virus Interactions

AbstractTapping into the metabolic cross-talk between a host and its virus can reveal unique strategies employed during infection. Viral infection is a dynamic process that generates an evolving metabolic landscape. Gaining a continuous view into the infection process is highly challenging and is limited by current metabolomics approaches, which typically measure the average of the entire population at various stages of infection. Here, we took a novel approach to study the metabolic basis of host-virus interactions between the bloom-forming algaEmiliania huxleyiand its specific virus. We combined a classical method in virology, plaque assay, with advanced mass spectrometry imaging (MSI), an approach we termed ‘in plaque-MSI’. Taking advantage of the spatial characteristics of the plaque, we mapped the metabolic landscape induced during infection in a high spatiotemporal resolution, unfolding the infection process in a continuous manner. Further unsupervised spatially-aware clustering, combined with known lipid biomarkers, revealed a systematic metabolic shift towards lipids containing the odd-chain fatty acid pentadecanoic acid (C15:0) induced during infection. Applying ‘in plaque-MSI’ might pave the way for the discovery of novel bioactive compounds that mediate the chemical arms race of host-virus interactions in diverse model systems.

scholarly journals In plaque-mass spectrometry imaging of a bloom-forming alga during viral infection reveals a metabolic shift towards odd-chain fatty acid lipids

2019 ◽  
Vol 4 (3) ◽  
pp. 527-538 ◽  
Author(s):  
Guy Schleyer ◽  
Nir Shahaf ◽  
Carmit Ziv ◽  
Yonghui Dong ◽  
Roy A. Meoded ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid ◽  
Viral Infection ◽  
Mass Spectrometry Imaging ◽  
Chain Fatty Acid ◽  
Metabolic Shift

scholarly journals MALDI Mass Spectrometry Imaging of Lipids and Gene Expression Reveals Differences in Fatty Acid Metabolism between Follicular Compartments in Porcine Ovaries

Biology ◽  
2015 ◽  
Vol 4 (1) ◽  
pp. 216-236 ◽  
Author(s):  
Svetlana Uzbekova ◽  
Sebastien Elis ◽  
Ana-Paula Teixeira-Gomes ◽  
Alice Desmarchais ◽  
Virginie Maillard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mass Spectrometry ◽  
Fatty Acid ◽  
Fatty Acid Metabolism ◽  
Mass Spectrometry Imaging ◽  
Acid Metabolism ◽  
Maldi Mass Spectrometry ◽  
Maldi Mass Spectrometry Imaging

scholarly journals Killer Archaea: Virus-Mediated Antagonism to CRISPR-Immune Populations Results in Emergent Virus-Host Mutualism

mBio ◽  
2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Samantha J. DeWerff ◽  
Maria A. Bautista ◽  
Matthew Pauly ◽  
Changyi Zhang ◽  
Rachel J. Whitaker
Keyword(s):  
Vertical Transmission ◽  
Natural Populations ◽  
Model Systems ◽  
Microbial Populations ◽  
Escape Mutation ◽  
Content Type ◽  
Sulfolobus Islandicus ◽  
Host Ecology ◽  
Host Virus Interactions ◽  
Virus Interactions

ABSTRACT Theory, simulation, and experimental evolution demonstrate that diversified CRISPR-Cas immunity to lytic viruses can lead to stochastic virus extinction due to a limited number of susceptible hosts available to each potential new protospacer escape mutation. Under such conditions, theory predicts that to evade extinction, viruses evolve toward decreased virulence and promote vertical transmission and persistence in infected hosts. To better understand the evolution of host-virus interactions in microbial populations with active CRISPR-Cas immunity, we studied the interaction between CRISPR-immune Sulfolobus islandicus cells and immune-deficient strains that are infected by the chronic virus SSV9. We demonstrate that Sulfolobus islandicus cells infected with SSV9, and with other related SSVs, kill uninfected, immune strains through an antagonistic mechanism that is a protein and is independent of infectious virus. Cells that are infected with SSV9 are protected from killing and persist in the population. We hypothesize that this infection acts as a form of mutualism between the host and the virus by removing competitors in the population and ensuring continued vertical transmission of the virus within populations with diversified CRISPR-Cas immunity. IMPORTANCE Multiple studies, especially those focusing on the role of lytic viruses in key model systems, have shown the importance of viruses in shaping microbial populations. However, it has become increasingly clear that viruses with a long host-virus interaction, such as those with a chronic lifestyle, can be important drivers of evolution and have large impacts on host ecology. In this work, we describe one such interaction with the acidic crenarchaeon Sulfolobus islandicus and its chronic virus Sulfolobus spindle-shaped virus 9. Our work expands the view in which this symbiosis between host and virus evolved, describing a killing phenotype which we hypothesize has evolved in part due to the high prevalence and diversity of CRISPR-Cas immunity seen in natural populations. We explore the implications of this phenotype in population dynamics and host ecology, as well as the implications of mutualism between this virus-host pair.

Identification of glycerophospholipid fatty acid remodeling by using mass spectrometry imaging in bisphenol S induced mouse liver

2018 ◽  
Vol 29 (8) ◽  
pp. 1281-1283 ◽  
Author(s):  
Chao Zhao ◽  
Peisi Xie ◽  
Ti Yang ◽  
Hailin Wang ◽  
Arthur Chi Kong Chung ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid ◽  
Mouse Liver ◽  
Mass Spectrometry Imaging ◽  
Bisphenol S
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