scholarly journals Real time portable genome sequencing for global food security

2018 ◽  
Author(s):  
Laura M. Boykin ◽  
Ammar Ghalab ◽  
Bruno Rossitto De Marchi ◽  
Anders Savill ◽  
James M. Wainaina ◽  
...  

AbstractThe United Nations has listed Zero Hunger as one of the 17 global sustainable development goals to end extreme poverty by 2030. Plant viruses are a major constraint to crop production globally causing an estimated $30 billion in damage 1 leaving millions of people food insecure 2. In Africa, agriculture employs up to 50% of the workforce, yet only contributes 15% to the GDP on average 3, suggesting that there is low productivity and limited value addition. This can be addressed through continued innovation in the fields of science and technology as suggested in the Science Agenda for Agriculture in Africa (S3A) 4. Sustainable management of plant viruses and their associated vectors must include efficient diagnostics for surveillance, detection and identification to inform disease management, including the development and strategic deployment of virus resistant varieties. To date, researchers have been utilizing conventional methods such as; PCR, qPCR, high throughput sequencing (RNA-Seq, DNA-Seq) and Sanger sequencing for pathogen identification. However, these methods are both costly and time consuming, delaying timely control actions. The emergence of new tools for real-time diagnostics, such as the Oxford Nanopore MinION, have recently proven useful for early detection of Ebola 6 and Zika 7,8, even in low resourced laboratories. For the first time globally, the MinION portable pocket DNA sequencer was used to sequence whole plant virus genomes. We used this technology to identify the begomoviruses causing the devastating CMD which is ravaging smallholder farmers’ crops in sub-Saharan Africa. Cassava, a carbohydrate crop from which tapioca originates, is a major source of calories for over eight hundred (800) million people worldwide. With this technology, farmers struggling with diseased crops can take immediate, restorative action to improve their livelihoods based on information about the health of their plants, generated using a portable, real-time DNA sequencing device.

2021 ◽  
Author(s):  
Arab Msume ◽  
Giulio Castelli ◽  
Faidess Mwale

<p>Agriculture is critical for human welfare and economic growth in Sub-Saharan Africa (SSA). However, especially rainfed agriculture remains vulnerable to the impacts of climate change in the region. This has generated increasing interest in practices such as Flood Based Farming Systems (FBFS) which enable turning flood water into an opportunity for crop production for smallholder farmers living in flood plains. Despite this interest, there is limited knowledge about farmers’ preference in terms of choices about a specific FBFS and therefore about which specific FBFS needs improvements for realizing its full benefits. The present study characterizes FBFS in Balaka District, Eastern Malawi, in order to develop a pilot approach for gaining knowledge and insights about farmers’ preferences. Data were collected from a sample of 398 questionnaires, direct observations, focus group discussions and key informant interviews, and they were analyzed through SPSS. Results show that Flood Recession Agriculture (FRA) was predominantly practiced (54%), together with other FBFS such as Depression Agriculture (DA), Spate Irrigation (SI) and Dug Outs (DO). Low capital investment and low level of awareness of farmers were referred to be critical for FRA adoption with (p<0.00003) and (p<0.004) respectively. Therefore, investing on FRA, which has already proven to be used in the area, could be a key to improve food security in the area. </p>


Author(s):  
Magnus Jirström ◽  
Maria Archila Bustos ◽  
Sarah Alobo Loison

This chapter provides a broad descriptive background of central aspects of smallholder agriculture in six countries in sub-Saharan Africa (SSA). It offers an up-to-date picture of the current trends of crop production, area productivity, levels of commercialization, and sources of cash incomes among 2,500 farming households. Structured around smallholder production, commercialization, and diversification in the period 2002–15, the chapter points on the one hand at persistent challenges such as low crop yields, low levels of output per farm, and a high degree of subsistence farming, and on the other hand at positive change over time in terms of growth in crop production and increasing levels of commercialization. It points at large variations not only between countries and time periods but also at the village levels, where gaps in crop productivity between farms remain large. Implicitly it points at the potential yet to be exploited in the SSA smallholder sector.


F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1101 ◽  
Author(s):  
Laura Boykin ◽  
Ammar Ghalab ◽  
Bruno Rossitto De Marchi ◽  
Anders Savill ◽  
James M. Wainaina ◽  
...  

Crop losses due to viral diseases and pests are major constraints on food security and income for millions of households in sub-Saharan Africa (SSA). Such losses can be reduced if plant diseases and pests are correctly diagnosed and identified early. Currently, accurate diagnosis for definitive identification of plant viruses and their vectors in SSA mostly relies on standard PCR and next generation sequencing technologies (NGS). However, it can take up to 6 months before results generated using these approaches are available. The long time taken to detect or identify viruses impedes quick, within-season decision-making necessary for early action, crop protection advice and disease control measures by farmers. This ultimately compounds the magnitude of crop losses and food shortages suffered by farmers. The MinION portable pocket DNA sequencer was used, to our knowledge globally for the first time, to sequence whole plant virus genomes. We used this technology to identify the begomoviruses causing the devastating cassava mosaic virus, which is ravaging smallholder farmers’ crops in sub-Saharan Africa.


2020 ◽  
Vol 12 (17) ◽  
pp. 6828 ◽  
Author(s):  
Essegbemon Akpo ◽  
Gebrekidan Feleke ◽  
Asnake Fikre ◽  
Mekasha Chichaybelu ◽  
Chris O. Ojiewo ◽  
...  

Sustaining crop production and productivity in sub-Saharan Africa requires the availability and use of quality seed of improved varieties by smallholder farmers. The private sector has been considered as the best way to sustain seed supply and crop productivity. Unfortunately, the private sector’s share in the seed production and delivery in sub-Saharan Africa countries has not been very substantial for decades. As a consequence, farmer access to quality seed of recently released varieties remains very low. This manuscript analyzes the experiences of informal seed producers who graduated to formal private seed enterprises to understand the effectiveness of the support they receive to become viable seed ventures. We used comparative research methods to analyze the qualitative and quantitative data collected to understand the underlying mechanisms. The findings showed that the analyzed seed enterprises started with as little as about USD 300 and have already multiplied over tenfold their initial capital. They benefited from a wide variety of supports, e.g., quality seed production, marketing, partnerships, and value chain development trainings and infrastructures, from extension workers, research centers, national and international NGOs, and the other private seed enterprise operators like large public seed enterprises and agro-dealers. The seed enterprises are producing pre-basic, basic, and certified seed of cereals and self-pollinated legume crops delivered directly to farmers, institutional markets, and agro-dealers. The seed production data have been increasing for the past three years with an area expanding from about 30 ha to over 150 ha per year for chickpea. The seed production and delivery practices being employed are smallholder farmer-based practices that are environmentally friendly. For sustainable and reliable seed production and delivery systems in sub-Saharan Africa, a bold step is needed whereby the informal seed production entities are nurtured and upgraded into formal certified seed production ventures that deliver social and economic benefits to the promotors and the communities.


2021 ◽  
Author(s):  
Douglas Gumbo ◽  
Menas Wuta ◽  
Isaiah Nyagumbo

Abstract Smallholder farmers in sub-Saharan Africa are increasingly exposed to risks such as erratic rainfall, prolonged dry spells, and frequent droughts that threaten sustainable crop production. This study assessed the effectiveness of dead level contours with innovations (DLC INN), dead level contours with infiltration pits (DLC INFIL), dead level contours with open channels (DLC OPEN) and standard graded contours (SGCs) in harvesting and storing water in the channel, improving crop conditions during the critical stages of maize growth on different soil textural groups in the Zvishavane District of Zimbabwe. The DLC INFIL, DLC OPEN and DLC INN outperformed the SGC under medium- and heavy-textured soils, with yields ranging between 1.7 and 2.36 t/ha compared to 0.9 t/ha for the SGC. For light textured soils, the DLC INN had the highest maize yield, averaging 0.8 t/ha. On heavy textured soils using DLC INN, DLC INFIL and DLC OPEN, smallholder farmers may use a spacing interval of 24–27 m. On medium textured soils, DLC INN and DLC INFIL can be used at a spacing interval of 18–21 m and 12 to 15 m with DLC OPEN. On light textured soils, farmers are advised to invest in DLC INN only, using a spacing interval of 12–15 m.


2021 ◽  
Vol 13 (3) ◽  
pp. 1158
Author(s):  
Cecilia M. Onyango ◽  
Justine M. Nyaga ◽  
Johanna Wetterlind ◽  
Mats Söderström ◽  
Kristin Piikki

Opportunities exist for adoption of precision agriculture technologies in all parts of the world. The form of precision agriculture may vary from region to region depending on technologies available, knowledge levels and mindsets. The current review examined research articles in the English language on precision agriculture practices for increased productivity among smallholder farmers in Sub-Saharan Africa. A total of 7715 articles were retrieved and after screening 128 were reviewed. The results indicate that a number of precision agriculture technologies have been tested under SSA conditions and show promising results. The most promising precision agriculture technologies identified were the use of soil and plant sensors for nutrient and water management, as well as use of satellite imagery, GIS and crop-soil simulation models for site-specific management. These technologies have been shown to be crucial in attainment of appropriate management strategies in terms of efficiency and effectiveness of resource use in SSA. These technologies are important in supporting sustainable agricultural development. Most of these technologies are, however, at the experimental stage, with only South Africa having applied them mainly in large-scale commercial farms. It is concluded that increased precision in input and management practices among SSA smallholder farmers can significantly improve productivity even without extra use of inputs.


2011 ◽  
Vol 47 (2) ◽  
pp. 205-240 ◽  
Author(s):  
JAMES W. HANSEN ◽  
SIMON J. MASON ◽  
LIQIANG SUN ◽  
ARAME TALL

SUMMARYWe review the use and value of seasonal climate forecasting for agriculture in sub-Saharan Africa (SSA), with a view to understanding and exploiting opportunities to realize more of its potential benefits. Interaction between the atmosphere and underlying oceans provides the basis for probabilistic forecasts of climate conditions at a seasonal lead-time, including during cropping seasons in parts of SSA. Regional climate outlook forums (RCOF) and national meteorological services (NMS) have been at the forefront of efforts to provide forecast information for agriculture. A survey showed that African NMS often go well beyond the RCOF process to improve seasonal forecast information and disseminate it to the agricultural sector. Evidence from a combination of understanding of how climatic uncertainty impacts agriculture, model-based ex-ante analyses, subjective expressions of demand or value, and the few well-documented evaluations of actual use and resulting benefit suggests that seasonal forecasts may have considerable potential to improve agricultural management and rural livelihoods. However, constraints related to legitimacy, salience, access, understanding, capacity to respond and data scarcity have so far limited the widespread use and benefit from seasonal prediction among smallholder farmers. Those constraints that reflect inadequate information products, policies or institutional process can potentially be overcome. Additional opportunities to benefit rural communities come from expanding the use of seasonal forecast information for coordinating input and credit supply, food crisis management, trade and agricultural insurance. The surge of activity surrounding seasonal forecasting in SSA following the 1997/98 El Niño has waned in recent years, but emerging initiatives, such as the Global Framework for Climate Services and ClimDev-Africa, are poised to reinvigorate support for seasonal forecast information services for agriculture. We conclude with a discussion of institutional and policy changes that we believe will greatly enhance the benefits of seasonal forecasting to agriculture in SSA.


2017 ◽  
Vol 5 (1) ◽  
pp. 50
Author(s):  
Kalifa TRAORE ◽  
Daouda SIDIBE ◽  
Harouna COULIBALY

Climate variability and change are recognized as the greatest challenge to crop production and food security in sub-Saharan Africa. This work assesses farmers’ perception on the contribution of improved varieties of sorghum and millet in the search for food security in Cinzana rural commune of Mali in the current context of climate change.The methodology was based on focus group surveys with both, the decentralized technical services, administrative and municipal authorities, NGOs, farmer organizations and producers but also farmer exchanges visits on improved varieties tested in farmer’s field.The result shows that climate change is described by the majority of farmers (87%) as decrease in rainfall amount and length of rainy seasons, high increases in temperature and high deforestation and water scarcity. Unpredictability of climate, (80%), drought (70%) and heavy rain (65%) occurrence were identified as major perception of farmers on risks in climate for crop production and soil degradation. After farmers’ study tour, 80% of the participants mentioned a better growth of plants and increase of soil moisture with the use of contour ridges tillage as a water conservation technology. Adapted cycle (55%) and higher yield (37%) of improved varieties were farmer’s main drivers for adoption of improved millet and sorghum varieties.The study revealed that local farmers have substantial knowledge on climate variabilities and risks and also are aware of some adaptation strategies. However, for wide scale adoption of effective strategies, capacity strengthening appeared a prerequisite.


2021 ◽  
Vol 10 (6) ◽  
pp. 48
Author(s):  
David Mhlanga

The study intended to investigate the factors that are important in influencing the financial inclusion of smallholder farming households in Sub-Saharan Africa with a specific focus on Zimbabwe. Motivated by the fact that there is an increase in the evidence of the importance of financial inclusion in fighting poverty and the fact that by merely having a bank account, financial inclusion cannot be guaranteed, the study went further to interrogate factors that influence smallholder farmers to have a transaction account, to borrow and to have insurance. Since the dependent variable of financial inclusion had more than two categories, with three unordered categories, transaction account, savings/credit account, and insurance, the multinomial logistic regression was used to estimate the determinants of financial inclusion from these three categories of the dependent variable. The multinomial logit model results, with insurance as the reference category, indicated that the size of the household, transaction costs, gender and agricultural extension service were the factors influencing the demand for a household to open a transaction account. On the other hand, off-farm income and age of the household were the only two factors significantly influencing households to borrow. Therefore, it is imperative for, the government of Zimbabwe to come up with more policies that encourage farmers to participate in the formal financial market as financial inclusion can help to fight poverty and the general developments of societies.   Received: 28 April 2021 / Accepted: 31 August 2021 / Published: 5 November 2021


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